Polysaccharide-based chiral HPLC
phases for better performance
Kromasil® AmyCoat™
Kromasil® CelluCoat™
Designed to stretch the limits.
Kromasil ® AmyCoat ™ and Kromasil ® CelluCoat ™ stretch the limits for analytical
and industrial chiral chromatography. The silica is based on an in-house developed
matrix and coated with a functionalized amylose or cellulose selector. High resolution,
excellent selectivity, no pressure limits* and stable performance when switching
between compatible mobile phases are some important benefits.
ANALYTICAL CHROMATOGRAPHY
N O P R E S S U R E L IM I T S
H I G H FL O W R A T E S
SMALL PARTICLES (3 μm)
FASTER ANALYSES
H I G H R E S O L U T IO N
U N I Q U E C O A T IN G M E T H O D
BETTER ANALYSES
E X C E L L E N T S E L E CT I V I T Y
BETTER ANALYSES
PREPARATIVE CHROMATOGRAPHY
U N I Q U E S IL I C A M A T R I X
M E C H A N I C A L L Y S T R ON G
LONGER LIFETIME
W I D E R A N G E OF P A R T I C L E S
C O N S I S T E NT P E R F O R M A N C E
U N I Q U E C O AT I N G M E T H O D
H I G H E R L OA D A B I L I T Y
* Kromasil AmyCoat and Kromasil CelluCoat can withstand flow rates equivalent to
pressures of up to 400 bar—i.e. the approximate limit for most HPLC systems.
EASIER TO SCALE UP
HIGHER PRODUCTIVITY
Peak performance. Expect nothing less.
To speed up and simplify method development, we have removed some of the
restrictions for coated polysaccharide phases. In analytical scale chromatography,
3 μm particles and the absence of pressure limits allow faster chromatography with
better results than ever before.
BETTER RESULTS
FASTER RESULTS
Both Kromasil AmyCoat and Kromasil CelluCoat
Not only can you get better results with Kromasil
show great enantioselectivity for many different
AmyCoat and Kromasil CelluCoat you also get
racemates. In a separate section of the brochure,
them faster. Thanks to a product characteristic
you will find an application guide showing exactly
like the absence of pressure limits, you can run
the performance levels you can expect.
analytical chromatography at very high flow
By having access to 3 μm particles, it enables
a higher plate count and resolution for analytical
rates and save time.
The high selectivity and 3 μm particles are also
chromatography. Combined with great selectivity,
important factors in speeding up the method. You
this facilitates the separation of the enantiomers.
achieve an improved resolution, which, in turn,
allows a higher flow rate.
Kromasil® AmyCoat™ and Kromasil® CelluCoat™
are available in particle sizes down to 3 μm.
Kromasil® AmyCoat™
Selectivity and resolution comparison—Kromasil AmyCoat 3 μm and Chiralpak AD-H (5 μm).
Kromasil AmyCoat, 3 μm
␣
Chiralpak AD-H
500
800 0
␣ = 1.5
Rs= 7.3
Rs= 5.8
605 0
000
400 0
0
50
205
0
0
0
5
10
15
[min.]
0
Common conditions
Solute: Carbinoxamine
Mobile phase: Heptane/2-Propanol/DEA (90/10/0.1)
Column size: 4.6 × 150 mm
5
10
.4
4
0
.3
0
.2
.5
␣ = 1.1
.4
Rs= 1.3
Particle size: 3 μm
Column length: 150 mm
Flow rate: 1 ml/min
0
AmyCoat 3 μm
Chiralpak AD-H*
Ambucetamide
1.4
1.4
4.8
4.2
Carbinoxamine
1.5
1.5
7.3
5.8
Ketoprofen
1.4
1.3
4.6
4.3
Naproxen
1.2
1.2
3.4
3.1
Oxamniquine
1.2
1.2
3.3
3.1
Proglumide
2.7
2.8
11.8
9.0
Sulindac
1.3
1.3
4.8
3.9
(* 5 μm)
Fast analytical chromatography
Particle size: 3 μm
Column length: 150 mm
Flow rate: 1 ml/min
␣ = 1.1
00
Rs= 2.1
00
Particle size: 3 μm
Column length: 150 mm
Flow rate: 2 ml/min
= 1.8
␣ =␣2.3
= 6.9
s
Rs=R4.7
.3
00
00
00
00
.1
00
00
0
0
␣ = 1.7
Rs= 3.5
00
Temperature: 25 °C
Detection: UV 220 nm
Baseline separation by
using 3 μm particles
00
0
0
Common conditions
Solute: trans-2-Phenyl-1-cyclohexanol
Mobile phase: Heptane/2-Propanol (95/5)
Column diameter: 4.6 mm
Particle size: 3 μm
Column length: 150 mm
Flow rate: 4.5 ml/min
00
␣␣= =2.21.8
R = 5.6
Rs=s 3.8
00
00
00
.2
0
.1
15
[min.]
Rs
Chiralpak AD-H*
Flow rate: 0.5 ml/min
Temperature: 22 °C
Detection: UV 226 nm
Difference in resolution—Kromasil AmyCoat
3 μm vs. 5 μm.
Particle size: 5 μm
Column length: 150 mm
Flow rate: 1 ml/min
AmyCoat 3 μm
␣ = 1.5
2
4
6
[min.]
Common conditions
Solute: Tröger’s base
Mobile phase: Heptane/2-Propanol/DEA (90/10/0.1)
Column diameter: 4.6 mm
0
0
1
2
3
[min.]
0 0.75 1.5
[min.]
Temperature: 25 °C
Detection: UV 220 nm
Baseline separation in
less than 1 minute
Kromasil® CelluCoat™
Selectivity and resolution comparison—Kromasil CelluCoat 3 μm and Chiralcel OD-H (5μm).
Kromasil CelluCoat, 3 μm
500
␣ = 2.3
00
0
50
␣
Chiralcel OD-H
Rs= 13.2
400 0
CelluCoat 3 μm
␣ = 2.0
Rs= 8.3
305 0
200 0
00
0
105
50
0
0
0
2
4
6
8
[min.]
Common conditions
Solute: trans-Stilbene oxide
Mobile phase: Heptane/2-Propanol (90/10)
Column size: 4.6 x 150 mm
0
2
4
6
8
[min.]
Rs
Chiralcel OD-H*
CelluCoat 3 μm
Chiralcel OD-H*
Trans-Stilbene oxide
2.3
2.0
13.2
8.3
Benzoin
1.5
1.5
8.6
5.7
TFAE
2.9
2.9
14.7
11
Tröger’s base
1.4
1.4
3.7
2.7
Oxprenolol
5.6
5.5
18.1
15.1
Naproxen
1.2
1.2
2.9
2.2
Proglumide
2.0
2.0
7.6
3.2
* (5 μm)
Flow rate: 1 ml/min
Temperature: 25 °C
Detection: UV 229 nm
Fast analytical chromatography
Particle size: 5 μm
Column length: 250 mm
Flow rate: 1 ml/min
Particle size: 3 μm
Column length: 150 mm
Flow rate: 1 ml/min
Particle size: 3 μm
Column length: 50 mm
Flow rate: 1 ml/min
Particle size: 3 μm
Column length: 50 mm
Flow rate: 3 ml/min
Particle size: 3 μm
Column length: 50 mm
Flow rate: 7 ml/min
0
50
␣ = 2.2
00
␣ = 2.3
00
Rs= 9.0
50
Rs= 10.8
␣ = 2.3
00
␣ = 2.2
Rs= 4.7
50
Rs= 3.8
00
00
00
50
0
5
10
[min.]
Common conditions
Solute: trans-Stilbene oxide
Mobile phase: Heptane/2-Propanol (90/10)
Column diameter: 4.6 mm
0
0
5
0
50
0
0
␣ = 2.2
Rs= 3.0
00
00
50
5
0
0
5
10
[min.]
0
0
1
2
3
[min.]
0
0
1
[min.]
0
1
[min.]
Temperature: 25 °C
Detection: UV 229 nm
Baseline separation in
less than 30 seconds
Kromasil® AmyCoat™ and Kromasil® CelluCoat™
—faster results, better results.
Makes everyday work so much easier.
Kromasil AmyCoat and Kromasil CelluCoat let you perform method development without interference from restrictive parameters. Forget pressure limits, long equilibration
times and degradation in performance when switching from one compatible mobile
phase to another—they simply do not exist anymore.
NO PRESSURE LIMITS
SHORT EQUILIBRATION TIMES
User-friendliness, as we see it, is the ability to
Column equilibration is a time-consuming activity
perform method development without being
when running chiral chromatography. Higher
restricted by various parameters. One well-known
flow rates allow Kromasil AmyCoat and Kromasil
restriction for coated polysaccharide phases is the
CelluCoat to show substantially shorter equilibration
general pressure limit over the bed. Kromasil
times than other coated phases on the market.
AmyCoat and Kromasil CelluCoat withstand flow
In general, long equilibration times are most
rates equivalent to pressures up to 400 bar—which
pronounced when switching mobile phases
is about the limit for the HPLC system itself. This
containing basic additives to acidic additives or
makes it possible to run analytical chromatography
the other way around. The test with a Kromasil
a lot faster.
CelluCoat 3 μm column switching between two
compatible mobile phases shows how short
STABLE PERFORMANCE
equilibration times you can get.
From a stability perspective, you can run Kromasil
There are also two tests illustrating short
AmyCoat and Kromasil CelluCoat using compatible
equilibration times and additive switch for both
normal, polar organic and reversed mobile phases.
Kromasil AmyCoat and Kromasil CelluCoat with
Switching between compatible normal to polar
absolutely no sign of memory effects.
organic mobile phases will not lead to any
degradation in performance, no need for solvent
dedicated columns.
To show the stability in column efficiency for
Kromasil CelluCoat, we have performed mobile
phase switches and visualized them after each
switch with a Knox plot.
Kromasil® AmyCoat™
Stable performance—No pressure limits—Freedom to switch solvents
Identical result
Identical result
After
Before
Test sequence
Solute: trans-Stilbene oxide
00
0
␣ = 3.1
Rs= 15.5
00
0
00
0
0
0
5
Mobile phase
Solute: trans-Stilbene oxide
Flow rate
[ml/min]
Run time
[h]
Total back-pressure
[bar]
1. Heptane/2-Propanol (90/10)
4.3
2
345
2. 2-Propanol
0.7
2
260
3. Acetonitrile
1
2
58
4. Ethanol
1
2
188
5. Heptane/2-Propanol (90/10)
1
2
72
00
00
00
0
10
[min.]
Common conditions
Stationary phase: Kromasil AmyCoat, 3 μm
Column size: 4.6 × 150 mm
Temperature: 25 °C
0
Mobile phase: Heptane/2-Propanol (90/10)
Flow rate: 1 ml/min
Detection: UV 229 nm
Short equilibration times—Freedom to switch additives
Identical result
Identical result
Mobile phase 1
Solute: Carbinoxamine
Detection: UV 228 nm
00
Mobile phase 2
Solute: Ketoprofen
Detection: UV 250 nm
00
␣ = 1.5
Mobile phase 1
Solute: Carbinoxamine
Detection: UV 228 nm
␣ = 1.2
50
Rs= 5.0
00
Rs= 3.4
00
␣ = 1.5
Rs= 5.0
00
00
Equilibration
Mobile phase 2 50
Duration: 18 min
0
00
0
0
2
4
[min.]
Common conditions
Stationary phase: Kromasil AmyCoat, 3 μm
Column size: 4.6 x 150 mm
␣ = 3.1
Rs= 15.6
Equilibration 00
Mobile phase 1
Duration: 12 min
0
0
2
4
6
8
[min.]
Mobile phase 1: Heptane/2-Propanol/DEA (90/10/0.1)
Mobile phase 2: Heptane/2-Propanol/TFA (90/10/0.1)
0
Flow rate: 2 ml/min
Temperature: 25 °C
2
4
[min.]
5
10
[min.]
Kromasil® CelluCoat™
8
120
selectivity
back−pressure
110
Reduced plate height
Change rel. start value [%]
Stable performance—No pressure limits—Freedom to switch solvents
100
90
7
6
5
Start
4
After 100% Acetonitrile (3h)
3
After 100% 2-propanol (3h)
2
1
0
80
0
10
20
30
40
0
50
1
time [h]
Conditions
Solute: trans-Stilbene oxide
Stationary phase: Kromasil CelluCoat, 3 μm
Column size: 4.6 x 50 mm
0.1
0.2
0.3
0.4
0.5
Common conditions
Solute: trans-Stilbene oxide
Stationary phase: Kromasil CelluCoat, 5 μm
Column size: 4.6 x 250 mm
Mobile phase: Heptane/2-Propanol (90/10)
Flow rate: 7 ml/min
Temperature: 25 °C
Identical result
Identical result
Mobile phase 1
Mobile phase 2
50
Mobile phase 1
50
50
= 2.3
00
= 1.4
00
Rs= 10.0
50
00
Equilibration
Mobile phase 1 50
Duration: 15 min
0
00
Equilibration
Mobile phase 2 50
Duration: 10 min
0
00
50
0
0
2
0
4
[min.]
Common conditions
Solute: trans-Stilbene oxide
Detection: UV 229 nm
Stationary phase: Kromasil CelluCoat, 3 μm
= 2.2
Rs= 9.9
00
Rs= 2.6
50
50
1
2
0
3
[min.]
Column size: 4.6 x 150 mm
Mobile phase 1: Heptane/2-Propanol (90/10)
Mobile phase 2: Ethanol
2
4
[min.]
Flow rate: 2 ml/min
Temperature: 25 °C
Short equilibration times—Freedom to switch additives
Identical result
Identical result
Mobile phase 1
Solute: Naproxen
Detection: UV 226 nm
Mobile phase 2
Solute: Tröger’s base
Detection: UV 220 nm
00
= 1.2
00
Rs= 2.7
00
00
Mobile phase 1
Solute: Naproxen
Detection: UV 226 nm
= 1.4
Rs= 3.4
00
00
Equilibration
Mobile phase 2
00
Duration: 20 min
00
Equilibration
00
Mobile phase 1
Duration: 20 min 00
00
0
2
4
6
8
10
[min.]
Common conditions
Stationary phase: Kromasil CelluCoat, 3 μm
Column size: 4.6 x 150 mm
= 1.2
Rs= 2.6
00
00
0
0.6
Reduced velocity
Short equilibration times—Freedom to switch solvents
0
= 2.2
= 2.1
= 2.1
0
0
2
4
6
8
[min.]
Mobile phase 1: Heptane/2-Propanol/TFA (90/10/0.1)
Mobile phase 2: Heptane/2-Propanol/DEA (90/10/0.1)
0
Flow rate: 1 ml/min
Temperature: 25 °C
2
4
6
8
10
[min.]
Mobile phase: Heptane/2-Propanol (90/10)
Flow rates: 0.1-1.2 ml/min
Temperature: 25 °C
Works all the way.
Kromasil products are well known for their ability to work along the whole spectrum
from analytical to industrial scale chromatography. Kromasil AmyCoat and Kromasil
CelluCoat are no exception.
SIMPLIFIES METHOD DEVELOPMENT
4000
With particle sizes from 3 μm to 10 μm giving
3500
identical selectivity, Kromasil AmyCoat and Kromasil
excellent performance.
As for all Kromasil products, you can perform
the required method development in analytical scale
columns and then scale up to a larger column.
You can, for example, use 3 μm particles in
an analytical scale column and translate all the
generated data to a larger column packed with
10 μm particles.
If you plan to scale up the process, we suggest
you use an analytical column packed with 10 μm
particles right from the start.
0
B a c k -p r e s s u r e i n c r e a s e [ % ]
CelluCoat make it easy to scale up while retaining
Kromasil CelluCoat, 10 μm (7.2 bar*)
3000
Kromasil AmyCoat, 10 μm (9 bar*)
2500
Chiralcel OD, 20 μm (0.8 bar*)
2000
Chiralpak AD, 20 μm (0.8 bar*)
1500
(* initial bed pressure)
1000
500
0
1
(70 bar)
2
(70 bar)
3
(70 bar)
4
(70 bar)
5
6
(100 bar) (100 bar)
Cycles of packing (piston pressure)
N.B. The relative back-pressure increase is a measure of the degree of
degradation of the material after repeated packings. Actual particle size for
Chiralcel and Chiralpak is about 3 times larger than that for Kromasil, which
explains the difference in initial back pressure (back-pressure is inversely
proportional to the square of the particle size).
The test was designed to exert greater than
0
normal mechanical stress on the chiral stationary
Easy to scale up
phases, and is performed at a packing pressure
0
above the maximum 50 bar recommended by the
manufacturer of Chiralcel OD and Chiralpak AD.
0
0
5
10
15
[min.]
3-CelluCoat
5-CelluCoat • Kromasil 10-CelluCoat
• ␣Kromasil
• ␣Kromasil
= 2.4 R = 13.9
= 2.4 R = 11.4
␣ = 2.4 R = 10.0
s
Common conditions
Column size: 4.6 × 150 mm
Mobile phase: Heptane/2-Propanol (90/10)
Flow rate: 0.5 ml/min
s
s
FULLY BACK-INTEGRATED
We manufacture the super wide pore silica for
Kromasil polysaccharide products and perform all
Temperature: 25 °C
Solute: trans-Stilbene oxide
Detection: UV 229 nm
subsequent steps leading to the final product. This
means you get a product with full traceability.
Every manufacturing step is ensured through our
MECHANICALLY STRONG
detailed quality system, and the final product is
Mechanical strength is an important product
never released until it has passed our rigorous
lifetime parameter. Kromasil AmyCoat and
quality control tests. Having all the manufacturing
Kromasil CelluCoat have a mechanically strong
steps in-house also gives us a strong position in
spherical silica which withstands repeated
terms of production capacity.
cycles of packing.
Kromasil® AmyCoat™
Carbinoxamine
Mobile phase: Heptane/2-Propanol/DEA (90/10/0.1)
Flow rate: 0.7 ml/min
Preparative applications
Analytical injection
Sample size: 10 μg
Detection: UV 254 nm
Preparative injection
Sample size: 7.5 mg
Detection: UV 280 nm
00
00
␣ = 1.5
00
00
00
00
00
00
0
0
0
5
10
0
15
[min.]
5
Purity [%]
Yield [%]
Warfarin
Mobile phase: Heptane/2-Propanol (85/15)
Flow rate: 1 ml/min
Equivalent loading on a
50 × 250 mm column:
Analytical injection
Sample size: 8.5 μg
Detection: UV 230 nm
10
Enantiomer 1
100
99.7
0.9 g
15
[min.]
Enantiomer 2
99.7
100
Preparative injection
Sample size: 17.1 mg
Detection: UV 325 nm
50
0
00
0
50
00
␣ = 3.3
00
00
00
50
0
0
0
10
20
30
[min.]
0
5
Purity [%]
Yield [%]
Common conditions
Stationary phase: Kromasil AmyCoat 10 μm
Column size: 4.6 x 250 mm
10
15
20
25
Enantiomer 1
99.7
99.4
2.0 g
30 35
[min.]
Enantiomer 2
99.5
99.7
Temperature: 25 °C
EASY TO SCALE UP
Conditions:
Column size: 4.6 × 150 mm
Flow rate: 0.5 ml/min
500
Conditions:
DAC system: NovaSep Pack-n-Sep, 50 mm i.d.
Bed length: 135 mm
Flow rate: 60 ml/min
200 0
␣ = 1.4
00
0
50
00
5 0
80
Rs= 2.6
␣ = 1.3
500
Rs= 2.4
00
0
50
600 0
00
0
0
5
10
15
20
[min.]
Common conditions
Stationary phase: Kromasil AmyCoat, 10 μm
Mobile phase: Heptane/2-Propanol (90/10)
Solute: Trifluoro-anthrylethanol
␣ = 2.9
Rs= 9.9
5
10
Detection: UV 254 nm
Temperature: 20 °C
15
20
[min.]
␣ = 3.0
Rs= 7.9
5
20
0
0
000 0
805 0
60
0 0
40
50
0
0
Conditions:
DAC system: NovaSep Pack-n-Sep, 50 mm i.d.
Bed length: 132 mm
Flow rate: 60 ml/min
00
0
40
5
20
50
Conditions:
Column size: 4.6 × 150
Flow rate: 0.5 ml/min
0
0
5
10
15
20
25
30 35
[min.]
Common conditions
Stationary phase: Kromasil CelluCoat, 10 μm
Mobile phase: Heptane/2-Propanol (90/10)
Solute: Trifluoro-anthrylethanol
0
5
10
15
Detection: UV 254 nm
Temperature: 20 °C
20
25
30 35
[min.]
Kromasil® CelluCoat™
Loadability comparison
Kromasil CelluCoat, 10 μm
Chiralcel OD, 10 μm
Analytical injection: 20 μg
Kromasil CelluCoat, 10 μm
Analytical injection: 20 μg
0
60
␣ = 1.2
00
00
40
0
Preparative injection: 5 mg
Collected fractions size: 0.5 min
00
␣ = 1.3
0
Chiralcel OD, 10 μm
Preparative injection: 5 mg
Collected fractions size: 0.5 min
00
00
00
0
20
00
0
00
00
0
0
0
5
10
15
20
25
[min.]
0
0
5
10
15
20
25
[min.]
0
0
5
10
15
20
25
[min.]
0
5
10
Enantiomer 1
Kromasil CelluCoat
Daicel Chiralcel OD
Common conditions
Solute: Naproxen
Mobile phase: Heptane/2-Propanol/TFA (90/10/0.1)
Column size: 4.6 x 250 mm
Purity
[%]
91.2
91.4
Purity
[%]
94.4
96.6
Binaphtol
Mobile phase: Heptane/2-Propanol (90/10)
Preparative applications
Preparative injection
Sample size: 1 mg
Detection: UV 350 nm
␣ = 1.2
00
0
0
00
0
0
0
0
0
5
10
15
20
25
[min.]
5
0
Purity [%]
Yield [%]
Metoprolol
Mobile phase: Heptane/2-Propanol/DEA (90/10/0.1)
Equivalent loading on a
50 × 250 mm column:
0
0
00
Analytical injection
Sample size: 10 μg
Detection: UV 223 nm
10
15
Enantiomer 1
95.4
91.7
20
25
[min.]
0.1 g
Enantiomer 2
97.8
72.8
Preparative injection
Sample size: 20 mg
Detection: UV 290 nm
00
0
00
␣ = 2.4
00
00
00
00
00
0
0
0
5
10
15
20
[min.]
0
Purity [%]
Yield [%]
Common conditions
Stationary phase: Kromasil CelluCoat, 10 μm
Column size: 4.6 x 250 mm
5
10
Enantiomer 1
100
97.9
Temperature: 25 °C
Flow rate: 1 ml/min
15
20
[min.]
Enantiomer 2
99.7
88.1
20
25
[min.]
Enantiomer 2
Yield
[%]
73.3
46.7
Temperature: 25 °C
Flow rate: 0.5 ml/min
Analytical injection
Sample size: 10 μg
Detection: UV 223 nm
15
2.4 g
Yield
[%]
50.1
24.9
Taking a closer look.
Kromasil® AmyCoat™
CHIRAL SELECTOR
Before testing conditions not mentioned,
The coated selector is tris-(3,5-dimethylphenyl)
please contact the Kromasil application group,
carbamoyl amylose.
[email protected]
PRODUCT ASSORTMENT*
3 μm - AmyCoat
5 μm - AmyCoat
10 μm - AmyCoat
4.6
x
50 mm
Kromasil 3-AmyCoat
4.6 x 50 mm
Kromasil 5-AmyCoat
4.6 x 50 mm
___
4.6
x
150 mm
Kromasil 3-AmyCoat
4.6 x 150 mm
Kromasil 5-AmyCoat
4.6 x 150 mm
Kromasil 10-AmyCoat
4.6 x 150 mm
4.6
x
250 mm
Kromasil 3-AmyCoat
4.6 x 250 mm
Kromasil 5-AmyCoat
4.6 x 250 mm
Kromasil 10-AmyCoat
4.6 x 250 mm
10
x
250 mm
___
Kromasil-5-AmyCoat
10 x 250 mm
Kromasil-10-AmyCoat
10 x 250 mm
21.2
x
250 mm
___
Kromasil 5-AmyCoat
21.2 x 250 mm
Kromasil 10-AmyCoat
21.2 x 250 mm
HN
O
O
HN
O
O
*
O
O
O
O
O
NH
HN
O
O
O
O
O
HN
NH
O O
*
n
tris-(3,5-dimethylphenyl)carbamoyl amylose
PARTICLE SIZES
* Other column dimensions available upon request.
PRODUCT CODES
Kromasil X-AmyCoat followed by column diameter
and length. X indicates particle size: 3, 5 or 10 μm.
For example: Kromasil 3-AmyCoat 4.6 x 150 mm
Kromasil AmyCoat is available in 3 and 5 μm as
pre-packed columns, and in 10 μm as pre-packed
column and bulk material.
COMPATIBLE MOBILE PHASES
0
10
20
30
40
50
60
70
80
90
2-Propanol in Alkane
Ethanol in Alkane
Methanol in Alkane*
2-Propanol in Acetonitrile
Methanol in Ethanol
Methanol in Acetonitrile
Methanol in CO2 (SFC)
*Due to limited miscibility of methanol in alkanes, ethanol should
be added as a mediator when exceeding 5% methanol.
Compatible conditions
Further investigations required
100 %
ORDERING INFORMATION
Order online at:
www.kromasil.com/online
[email protected]
Or contact:
Eka Chemicals, Separation
Products,
SE-445 80 Bohus, Sweden
Tel. +46 31 58 73 60
Fax. +31 58 77 27
Kromasil® CelluCoat™
CHIRAL SELECTOR
Before testing conditions not mentioned,
The coated selector is tris-(3,5-dimethylphenyl)
please contact the Kromasil application group,
carbamoyl cellulose.
[email protected]
PRODUCT ASSORTMENT*
3 μm - CelluCoat
5 μm - CelluCoat
10 μm - CelluCoat
4.6
x
50 mm
Kromasil 3-CelluCoat
4.6 x 50 mm
Kromasil 5-CelluCoat
4.6 x 50 mm
___
4.6
x
150 mm
Kromasil 3-CelluCoat
4.6 x 150 mm
Kromasil 5-CelluCoat
4.6 x 150 mm
Kromasil 10-CelluCoat
4.6 x 150 mm
4.6
x
250 mm
Kromasil 3-CelluCoat
4.6 x 250 mm
Kromasil 5-CelluCoat
4.6 x 250 mm
Kromasil 10-CelluCoat
4.6 x 250 mm
10
x
250 mm
___
Kromasil-5-CelluCoat
10 x 250 mm
Kromasil-10-CelluCoat
10 x 250 mm
21.2
x
250 mm
___
Kromasil 5-CelluCoat
21.2 x 250 mm
Kromasil 10-CelluCoat
21.2 x 250 mm
HN
O
*
NH
O
HN
O
O
O
O
O
O
O
O
O
*
O
O
NH
HN
O
O
n
O
HN
tris-(3,5-dimethylphenyl)carbamoyl cellulose
PARTICLE SIZES
* Other column dimensions available upon request.
PRODUCT CODES
Kromasil X-CelluCoat followed by column diameter
and length. X indicates particle size: 3, 5 or 10 μm.
For example: Kromasil 3-CelluCoat 4.6 x 150 mm
Kromasil CelluCoat is available in 3 and 5 μm as
pre-packed columns, and in 10 μm as pre-packed
column and bulk material.
COMPATIBLE MOBILE PHASES
0
10
20
30
40
50
60
70
80
90
2-Propanol in Alkane
Ethanol in Alkane
Methanol in Alkane*
MTBE in Alkane
MTBE in Ethanol
Methanol in Acetonitrile
Methanol in CO2 (SFC)
*Due to limited miscibility of methanol in alkanes, ethanol should
be added as a mediator when exceeding 5% methanol.
Compatible conditions
Further investigations required
100 %
The moment you adopt our Kromasil High Performance Concept, you join thousands
of chromatographers who share a common goal: to achieve better separations
when analyzing or isolating pharmaceuticals or other substances.
Not only will you benefit from our patented silica technology, but you gain a
strong partner with a reliable track record in the field of silica products. For the past
60 years, Eka Chemicals has pioneered new types of silica. Our long experience in
the field of silica chemistry is the secret behind the development of Kromasil, and
the success of our Separation Products Group.
Kromasil is available in bulk, or in high-pressure slurry-packed columns.
The development, production and marketing of Kromasil are ISO 9001 certified.
Eka Chemicals is a global company with 3,000 people in 30 countries. It is a
business unit within Akzo Nobel, one of the world’s largest chemical groups, with
more than 67,000 employees in 80 countries.
Eka Chemicals AB
Separation Products
SE-445 80 Bohus, Sweden
Telephone + 46 31 58 73 60
Fax + 46 31 58 77 27
For NAFTA countries:
Akzo Nobel/Kromasil
281 Fields Lane, Brewster
NY 10509-2676, USA
Telephone + 1 845 276 8223
[email protected]
www.kromasil.com
Kromasil®
® Eka Chemicals AB 2007
This publication may not be reproduced in any way without the consent of Eka Chemicals AB.