Bipolaris Leaf Spot and Brown Patch Control in a Mixed Stand of Ryegrass and Tall Fescue with Heritage and Headway Using Lawn Applicator Technique, 2007 Materials and Methods: This study was conducted in front of the ‘Research Tee’ at Philadelphia Country Club, located in Gladwyne, Pennsylvania. The stand was a mix of tall fescue and perennial ryegrass and maintain similar to a high-end lawn. No fungicides were applied to the turf the spring prior to the initiation of the study. Site was supplied with 1.5 lb N/1000 ft2 on 19 May and again with 0.5 lb N/1000 ft2 from 19-25-5 Anderson’s Contec. Soil was a native clay-loam with a pH of 6.6 and 4.3% organic matter. Turf was mowed three times per week with a Z-turn rotary mower at three inches. Irrigation was applied to the area as needed to prevent drought stress and occurred in the early evening when preformed. On treatment days, materials were applied to a dry canopy using a lawn care simulation application technique. Mowing and irrigation was restricted for 48 hours following all applications. Plots were 7.5 ft x 5 ft and arranged in a randomized complete block with four replications. Fungicides were applied using an FMC spray gun calibrated to deliver 2.0 gallons 1000ft2, with relatively large droplets. Treatment application rates and dates are footnoted in table 1. Ratings were taken when disease and turf quality differences were observed within the study. Data were subjected to analysis of variance (ANOVA) using ARM and significantly different means were separated according to the Least Significant Difference (LSD) test (P ≤0.05). Results: Leaf spot, caused by Bipolaris species, was microscopically identified in the site on 14 June. All fungicide treated plots (0.0- 2.0%) had less blighting from the leaf spot, when compared to the untreated control (29%). Similar trend was observed on 29 June, in which all treated plots had significantly less blighting from leaf spot, than the untreated control. Also on that date, plots treated with Headway at 1.0 fl oz/1000ft2 had an average of 3.5% blighted while all other fungicide treated plots were disease-free. All plots were re-treated on 1 July. Brown patch blighting did not occur until 19 July. All treated plots remained disease-free until 16 August (approximately 45 days since last treatment). Brown patch blighting in the untreated control was 18.0, 27.0 and 41.0, on 26 July, 2 August and 10 August, respectively. On 16 August, all fungicide-treated plots had less brown patch blighting than the untreated control, however, the least amount of disease was observed in plots treated with Headway at 1.5 fl oz/1000ft2. Plots treated with Headway at 1.0 oz/1000ft2 had significantly more blighting, when compared to plots treated with Headway at 1.5 fl oz/1000ft2. In a previous study conducted with same application in 2006, close to 45 days of control was achieved with Headway applied at 1.5 fl oz/1000ft2. While other treated plots had minor levels of blighting. Data from this years’ trial and the pilot trial conducted in 2006 suggest that lawn care applicators could achieve acceptable and cost effective levels of brown patch control on a mixed stand of tall fescue, fine fescue and perennial ryegrass with two properly timed fungicide applications. Another benefit of these applications would be the control of the endemic disease, leaf spot. Overall turfgrass quality was rated three times over the course of the trial. On 29 June, while leaf spot blighting was occurring in the untreated plots, all fungicide treated plots had significantly higher and agronomically great quality. On 19 July, and when brown patch blighting occurred, a similar trend was observed. On the 10 August, rating date, the quality in the untreated control had dropped to 5.0 and was poor due to color and blighting by brown patch. All fungicide-treated plots had acceptable levels of quality and should be considered commercially acceptable for a high-end lawn care situation. No significant differences were observed among fungicide treatments for their level of quality. Table 1. Leaf spot and brown patch control in 3 inch mixed perennial ryegrass and tall fescue as influenced by various fungicide application % Plot Area Blight by Bipolaris y % Plot Area Blighted By R. Solani y Rate 14 June 29 June 19 July 26 July 2 August 10 August 16 August (oz/1000ft2) Headway 1.0 fl 2.0 b z 3.5 b 0.0 b 0.0 b 0.0 b 0.0 b 5.5 b Headway 1.5 fl 0.0 b 0.0 b 0.0 b 0.0 b 0.0 b 0.0 b 0.0 c Heritage WG 0.2 oz 2.0 b 0.0 b 0.0 b 0.0 b 0.0 b 0.0 b 1.5 bc Heritage TL 1.0 fl 0.0 b 0.0 b 0.0 b 0.0 b 0.0 b 0.0 b 1.5 bc Untreated 29.0 a 33.3 a 12.5 a 18.0 a 27.0 a 41.0 a 36.0 a P>F 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 x All treatments were applied on 25 May 2007. The 28-day sequential treatments were re-applied 1 July, 2007. All treatments were applied using a lawn-care applicator gun using large droplets in 2 gallons of water per 1000 ft2 at 38 psi. y Percent of plot area blighted was visually assessed on a 0 to 100 scale, with 0= no blighting and 100=entire plot area blighted. z Means in each column followed by different letters are significantly different (P ≤0.05) according to the Fischer’s Protected least significant difference test. Treatment x Table 2. Three Inch Turf Type Tall Fescue Quality as Influenced by Various Fungicides and Application Intervals, 2006. Treatment Headway Headway Heritage WG Heritage TL Untreated P>F x 2 Rate (oz/1000ft ) 1.0 fl 1.5 fl 0.2 oz 1.0 fl - 29 June 9.00 a 9.13 a 9.13 a 9.13 a 6.83 b 0.0001 Overall turfgrass quality (0-10) y 19 July 9.13 a 9.25 a 9.13 a 9.13 a 6.15 b 0.0001 10 August 9.00 a 9.00 a 9.00 a 9.08 a 5.00 b 0.0001 All treatments were applied on 25 May 2007. The 28-day sequential treatments were re-applied 1 July, 2007. All treatments were applied using a lawn-care applicator gun using large droplets in 2 gallons of water per 1000 ft2 at 38 psi. y Turfgrass quality was rated on a 1- 10 scale with 7.7= the minimal acceptable level for a tall fescue lawn in the mid-Atlantic and 10= optimal greenness, density and appearance. z Means in each column followed by different letters are significantly different (P ≤0.05) according to the Fischer’s Protected least significant difference test. Date 8/ 10 / 07 07 07 20 07 00 7 20 20 20 /2 27 / 20 / 13 / 8/ 3 7/ 7/ 7/ 07 07 00 7 20 20 /2 29 / 22 / 07 00 7 00 7 20 /2 /2 15 / 7/ 6 6/ 6/ 6/ 6/ 8 6/ 1 Degree F and % Average Ambient Air Temperature and Humidity, Gladwyne, PA 2007. 100 90 80 70 60 50 Ave temp %Humidity 40 30 20 10 0
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