International Journal of Mechanical & Mechatronics Engineering IJMME-IJENS Vol:14 No:06 39 Effect of Mesosphilic and Thermophilic Temperature on Floating drum anaerobic bio-digester S. Sathish1, ª* S. Vivekanandan2, b 1 Research Scholar, Department of Mechanical Engineering, AnnamalaiUniversity, India Associate Professor, Department of Mechanical Engineering, Annamalai University, India. Email: [email protected] , [email protected] 2 Abstract-- This work examines the biogas yield and methane production was performed using swine manure. The anaerobic digestion process was conducted in floating drum type biodigester with capacity of 1m3 made of fiber material. The codigestion of cow dung+swine manure in the ratio of 1:2 waste/water as 30:60 and waste by volume as 70:30 was added in the bio-digester. The main purpose of the research is to measure the biogas production and methane in mesosphilic and Thermophilic conditions. These results indicated that biogas yield 420 Liters and 460 Liters in mesosphilic and thermophilic conditions respectively. The maximum biogas production reported as 30min in thermophilic condition using inflammable time. Here thermophilic anaerobic digestion having high methane production. It’s found 62.8% and mesosphilic condition for 59% then pH value also higher for thermophilic condition. The biogas yield and methane is peak and quick at shorter retention time in thermophilic temperature 56°c. Index Term-Anaerobic Digestion, Floating Drum, Inflammable time, Thermophilic Condition, Mesophilic Condition, Retention Time, Fiber material. 1. I NTRODUCTION The anaerobic fermentation processes mainly used for organic wastes are eliminating to organic pollutants, and also eliminate the organic waste volume. So this biogas generation is very profitable one [1].Biogas is produced by anaerobic fermentation of organic wastes, manures and agricultural wastes and energy crops. This biogas is a type of biofuel [2]. Biogas is mainly based on microorganisms and other parameters like temperature, acid production (pH), HRT, Carbon\Nitrogen ratio, etc., [3]. Now a day’s continuously increases the amount of generated solid waste act to the large environment as impacts [4]. This anaerobic process is needed for biogas generation where methanogenic formed on swine manure (or) animal waste [5]. The composition of biogas various depending upon the origin of the anaerobic fermentation process and composition of biogas are 50-70% Methane (CH4), 30-40% Carbon dioxide (CO2), 0-1% Hydrogen (H2), 0-10% Nitrogen (N2), 0-3% Hydrogen Sulphide (H2s), 0-0 Oxygen (O2) [6]. In this process is commonly used in anaerobic bio digester and most popular type for floating drum anaerobic bio digester [7]. A possibility of analyzing and optimizing the biogas generation and both operating temperature’s from swine manure by serial fermentation process was studied. The co-digestion of cow manure + swine manure is creating an environmental benefit and biogas also used as a fuel in automobile for near future. It’s one of the renewable energy from biomass and biogas slurry is used to increase the fertilizer quality of animal waste [8]. The organic wastes are used to increase the quality of AD process. It’s mainly depends on good Carbon/Nitrogen ratio [9]. The swine manure is used mono substrate and low C/N balance in this condition AD process is Unstable [10]. Fig 1, illustrates the biogas generation mainly dependence on four different stages from the anaerobic digestion process. Conventional Anaerobic digestion (AD) is carried out at mesophilic temperature, which is 308-310 K. The composition of organic fraction of MSW is influenced by several factors, including regional differences, climate, and collection frequency as well as changes in composition [11]. Environmental LCA studies of AD of pig and cattle manure (raw or separated fraction) and energy crops such as maize and ryegrass focused on bio-energy production, greenhouse gas (GHG) emission reduction potentials, and various biogas end applications [12]. Main substrates for Anaerobic digestion include agricultural biomass in the form of animal manures and energy crops (e.g. Maize), organic residues from the processing industry (e.g. glycerin, beet tails, and gut and intestines from slaughtering houses) and other residues such as, road side grass or forest residues are used in this anaerobic digestion process of the experiment [13]. The thermophilic conditions resulted in a factor 1.3 higher biogas production rate than under mesophilic conditions for s.obliquus. The lower results of the digestion of s.obliquus at a mesophilic temperature can be species related. It has been reported that the strain can grow at temperatures up to 35°C optimized [14]. 142106-5959-IJMME-IJENS © December 2014 IJENS IJENS International Journal of Mechanical & Mechatronics Engineering IJMME-IJENS Vol:14 No:06 40 The main problem of thermophilic digestion (as a compared to the mesophilic digestion) include energy requirement and highly pollutant supernatant, all of which prevent this technique from being widely used and commercialized [17]. Generally biodigester can be operated in the temperature ranges a) Psychrophiles below 28°c b) Mesophilic medium temperature at 29°c to 45°c c) Thermophilic at 50 to 56°c [18]. Basically Mesophilic condition is more stable than thermophilic condition in anaerobic digestion process [19]. Pig manure and cow dung, the cheap and abundant animal wastes in Tamil nadu, so this experiment investigated to be applied as a raw material for the bio-energy production. In this research mainly focus on performance of the floating drum anaerobic biodigester in treating swine manure + cow manure. The biogas generation using two different temperature ‘s and then compared pH range ,biogas composition from both temperature ranges in four different stages like, hydrolysis, acidogenesis, acetogenesis and methanogenesis [20]. 2. METHODS AND M ATERIAL In this experiment were conducted using floating drum anaerobic bio- digester. The cow dung + swine manure feed as a bio-digester and it is one of the batch process and it’s used in two different temperatures mesophilic temperature(36°c) and thermophilic temperature(56°c) here cow dung is used as a inoculums’. The composition of biogas was carried out with a help of gas chromatography GA45. Then pH was measured weekly using pH redox meter and cumulative gas was measured with help of gas meter. The daily gas production was measured inflammation time using gas burner. Fig. 1. Different stages of anaerobic digestion process Separating the acidogenic and methanogenic steps in the anaerobic digestion process, provides enhanced stability to the different groups of microorganisms and better process control for the digestion process [15]. The effect of various parameters in general and total ammonia (free ammonia and ammonia – N) accumulation in particular upon thermophilic dry anaerobic digestion systems here ammonia – N and were accumulation in particular upon thermophilic dry anaerobic digestion system, which can affect the overall CH4 [16]. 3. EXPERIMENTAL AND METHODS Fig 2.shows the schematic view of the Experimental setup while Fig 3. Illustrate the Photo graphic view of the Experimental setup. The bio-digester with a capacity of 1m3 made up of fiber material floating drum type. The raw material cow dung and Fresh swine manure as taken from rural areas near Chidambaram town and this feeds are dried 1 week in sun light and crushed mechanically. 142106-5959-IJMME-IJENS © December 2014 IJENS IJENS International Journal of Mechanical & Mechatronics Engineering IJMME-IJENS Vol:14 No:06 41 GAS PILLOR PRESSURE GAUGE VALU 700 LITRES GAS COLLELTING GAS HOLDER BIOGAS GAS OUTLET BIOGAS DOME BURNER INLET PH PROBE HEATER WATRER TANK FEED STOCK INLET GAS FLOW METER STIRRER PUMP BIOGAS MEASUREMENT BY INFLAMMABLE TIME HOT WATER ( THERMO PHILLIC CONDITION ) (SLURY) FEEDSTOCIC PH OUTLET PROBE 700 LITRES GAS COLLELTING GAS HOLDER FEET STOCK OUTLET Fig. 2. Schematic view of the experimental setup Fig. 3. Photographic view of the experimental setup Then feed to cow dung swine manure mixed in the ratio of 1:2 waste/water as 30:60 and waste by volume as 70:30 was added in the same quantity of water added in the bio-digester. The bio digester was operated in two temperature ranges mesophilic temperature range 36°c and thermophilic temperature range 56°c. Hot water is used to the thermophilic condition with the help of water jacket. The produced biogas is collected in airbag then composition was analyzed using gas chromatography GA45. Biogas production is monitored weekly one day. Pneumatic stirrer was used to agitate digester slurry and digested slurry was collected from inside bio digester then we used as an organic fertilizer here waste is convert to useful energy. Here both temperature conditions were measured using thermocouples device monitored daily. A digital Redox pH meter was used to determine the pH of the bio digester. The initial pH was adjusted to the optimum limit for the digester. In this case gas burner provided to measure using inflammation time with both condition of the experiment. Initial and final Pressure was measured using pressure gauge. Biogas production was calculated weekly 142106-5959-IJMME-IJENS © December 2014 IJENS IJENS International Journal of Mechanical & Mechatronics Engineering IJMME-IJENS Vol:14 No:06 42 from by gas flow meter. The digester inside temperature was maintained in electric heater which circulate the hot water in bio-digester and it is observed by the digester slurry in this process water jacket used as a heat exchanger. The final digested slurry was collected and used as an organic fertilizer. 4. RESULTS AND DISCUSSION Fig. 6. Cumulative Biogas production in seven weeks of digestion Fig. 4. Biogas production in seven weeks of digestion In Fig.6.Shows the productions of cumulative biogas in mesophilic and thermophilic condition was measured during seven weeks of digestion. The cumulative biogas production at different time interval for both temperature ranges. The cumulative biogas production in mesophilic condition at 49th day was 1770 liters and maximum cumulative biogas produced in thermophilic condition at 49th day was 2400 liters achieved, then retention time for cowdung+swine manure is last 15 days best for maximum production of biogas. Fig.4. Shows the productions of biogas yield in mesophilic and thermophilic condition was measured during seven-weeks of digestion. In thermophilic condition after fifth week of digestion gas production tends to increase then drops in the seventh week and mesophilic condition the gas formation was lower than thermophilic condition. The high quantity of biogas yield was produced in thermophilic temperature in sixth week of digestion. Major difference between the mesophilic and thermophilic condition biogas production occurs in last ten days of the experiment. Fig. 7. Acid production in 7weeks of digestion Fig. 5. Quantity of Biogas with inflammable time Fig.5. shows the digester performance of cow dung + swine manure was investigated under mesophilic and thermophilic condition based on the inflammable time of the Gas production. It was observed that biogas production was actually slow at starting and the end of observation. It’s observed that the maximum gas production reported as 30min in thermophilic condition. The peak value of daily gas production differs for both temperature conditions. The acid production mainly based on acetogenesis and methogenesis process. Here Fig.7.shows the acid production (pH) in mesophilic and thermophilic temperature from cowdung + swine manure, the pH tends to increase then drops from 7.5 to 7.4 in thermophilic condition and mesophilic condition where maximum pH formation occur at seventh week of digestion. The optimum pH for the production of biogas was found to be 7.4 to 7.5 in thermophilic temperature. This means pH tents to increase on the 35th day of digestion. Then the production of biogas reduced and process stopped at some point. Table I Biogas composition of co-digestion process Mesophilic temperature CH4 59% CO2 32.% O2 0.30% 142106-5959-IJMME-IJENS © December 2014 IJENS Thermophilic temperature CH4 62.8% CO2 37.50% O2 0.50% IJENS International Journal of Mechanical & Mechatronics Engineering IJMME-IJENS Vol:14 No:06 [6] [7] [8] [9] [10] [11] [12] Fig. 8. Biogas composition in mesophilic and thermophilic condition Fig.8.shows the biogas composition (CH4, CO2 & O2) was analyzed in course of the seven weeks of digestion with gas analyzed GA45.Table 1 represent the biogas composition of cowdung + swine manure in mesophilic and thermophilic temperature range. The average methane content ranged from 59% and 32% of CO2 content in mesophilic temperature range. The maximum level of CH4 was 62.8% and 37.50% of CO2 obtained in the seventh of digestion in thermophilic condition. Then the oxygen was under 1% of digestion in both conditions. The high quantity of biogas (methane) is generated in thermophilic temperature range is compare to mesophilic temperature range. 5. CONCLUSION The results show that the optimum temperature observed from experiment is at thermophilic range 56°c was achieving a higher biogas yield and high methane content compare to mesophilic temperature range and maximum burning time of gas production was 30min. The biogas yields ranged between 420 and 460 liters in mesophilic and thermophilic anaerobic digestion has operated at constant temperature 36°c and 56°c. The percentage of CH4 in biogas produced in thermophilic range is higher on mesophilic range. Generally the generation of biogas from anaerobic digestion process is dependent on the amount of acid formation and temperature. Thermophilic condition of energy plant at 56°c is truly more economical one. 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