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e 1 of 2
eBiosc
cience™ Concana
avalin A (C
Con A) S
Solution (5
500X)
Catalog Number: 00-4
N
4978 For Rese
earch Use On
nly. Not for use
u in diagno
ostic proced
dures.
pheral blood m
mononuclear ce
ells
Lefft: Human perip
we
ere labeled with
h 10 uM Cell Prroliferation Dye
e
eF luor® 450 (cat . 65-0842) and
d cultured for 3 days
witth Concanavaliin A (Con A) So
olution (500X) at 2
uL per mL of culture medium. C
Cells were stain
ned
witth Anti-Human CD86 PE (cat.. 12-0869) and
Fixxable Viability D
Dye eFluor® 66
60 (cat. 65-086
64).
Tottal singlet-gate
ed, viable cells were used for
ana
alysis.
ght: Mouse sple
enocytes were
e labeled with 10 uM
Rig
Ce
ell Proliferation Dye eFluor® 4
450 (cat. 65-08
842)
and
d cultured for 3 days with Concanavalin A (C
Con
A) Solution (500X
X) at 2 uL per m
mL of culture
edium. Cells we
ere stained with Anti-Mouse C
CD86
me
PE
E-Cyanine7 (ca
at. 25-0862) and
d Fixable Viabiility
Dyye eFluor® 660
0 (cat. 65-0864)). Total singlet-gatted, viable cellss were used fo
or analysis.
Product In
nformation
Contents: eBios
C
science™ Concanavalin A
(C
Con A) Solution
n (500X) Catalog
C
Numbe
er: 00-4978 Concentration:
C
: 500X (1.25 mg/mL)
Handling
H
Cond
ditions: Use in sterile
environment.
Source:
S
Canavllia ensiformis
Formulation: Sterile aqueou
us buffer, no so
odium
azide
a
T
Temperature Limitation: Sto
ore at -20°C.
Batch Code: R
Refer to vial Use By: Referr to vial
Descriptio
on
The Conca
anavalin A (Con
n A) Solution (5
500X) is a read
dy-to-use solutiion of Con A in
n aqueous buffe
er. Con A is isolated
from the Ja
ack bean, Cana
avlia ensiformis
s. It is a lectin that
t
binds alph a-D-glucose an
nd alpha-D-ma
annose moietie
es
found in va
arious glycopro
oteins, glycolipid
ds, and sugars
s and is a poten
nt leukocyte miitogen. This rea
agent is intended for
use in in viitro activation of
o human and mouse
m
leukocyttes.
ons Reported
Applicatio
Concanava
alin A (Con A) Solution
S
(500X
X) has been rep
ported for use i n in vitro culturres.
ons Tested
Applicatio
The activity
y of the Concanavalin A (Con
n A) Solution (5
500X) has been
n tested by proliferation of mo
ouse splenocyttes as
measured by dilution of Cell
C Proliferation Dye eFluor®
® 450. This is a pre-titrated 50
00X solution an
nd can be dilute
ed to 2
ormance in the assay of intere
est.
μL per mL of culture medium. This reagent may be furrther-titrated fo r optimal perfo
t
conditio
ons listed above, no change in performance
e is observed affter 20 freeze-tthaw cycles. Fo
or
Under the testing
optimal perrformance, sma
aller aliquots may
m be prepare
ed to minimize tthe number of freeze-thaw cyycles.
es
Reference
Dwyer JM, Johnson C. Th
he use of conc
canavalin A to study
s
the immu
unoregulation o
of human T cellls. Clin Exp
Immunol. 1981
1
Nov;46(2)):237-49.
de Petris S.
S Concanavalin
n A receptors, immunoglobuliins, and theta a
antigen of the llymphocyte surrface. Interactio
ons
with concanavalin A and with Cytoplasm
mic structures. J Cell Biol. 197
75 Apr;65(1):12
23-46.
Not for further
f
distrib
bution withoutt written conse
ent.
Copyrightt © 2016 Thermo Fisher Scientific Inc. All
A rights rese
erved. All trademarks are p
property of Th
hermo
Fisher Scientiffic and its sub
bsidiaries unle
ess otherwise
e specified.
Tel: 888.999.1371
8
1 or 858.642.2
2058 • Fax
x: 858.642.204
46 • thermo
ofisher.com/e
ebioscience •
info@e
ebioscience.co
om
14 March 2017 Rev. 10
0
e 2 of 2
Page
eBiosc
cience™ Concana
avalin A (C
Con A) S
Solution (5
500X)
Catalog Number: 00-4
N
4978 For Rese
earch Use On
nly. Not for use
u in diagno
ostic proced
dures.
hydrate interacttion. On the mo
ode of bonding
g of aromatic m
moieties to
Poretz RD,, Goldstein IJ. Protein-carboh
concanava
alin A, the phyto
ohemagglutinin
n of the jack be
ean. Biochem P
Pharmacol. 197
71 Oct;20(10):2
2727-39.
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Not for further
f
distrib
bution withoutt written conse
ent.
Copyrightt © 2016 Thermo Fisher Scientific Inc. All
A rights rese
erved. All trademarks are p
property of Th
hermo
Fisher Scientiffic and its sub
bsidiaries unle
ess otherwise
e specified.
Tel: 888.999.1371
8
1 or 858.642.2
2058 • Fax
x: 858.642.204
46 • thermo
ofisher.com/e
ebioscience •
info@e
ebioscience.co
om
14 March 2017 Rev. 10
0