From www.bloodjournal.org by guest on June 18, 2017. For personal use only.
Relation
of
Mastocytoina
Heparin,
By
Histamine
LTHOUGH
the
LENA
mtich
information
function
of these
leukocytes
has
staining
availability
about
The
debated
properties,
PAUL
of a readily
cells
relation
of tissue
their
Mast
AND
JACOB
has
regarded
the
transplantable,
well
available
as
about
to blood
of
blood
basophil
their
mast
differentiated,
in
is known
Because
latter
Cells
become
cells
of
FURTH
) , little
mast
discovery.
atid
to
Uvn#{228}s,2Riley1
since
Ehrlich4
Leukemia,
HAGEN
mast
by Padawer,’
cells.
been
Cell
Serotoniti
Z0MPETrI,
( reviews
years
Mast
and
ONo,
SABURO
recent
to
similar
cells.
The
slow-growing
mast
cell tumor
containing
large
quantities
of heparin,
histamine
and
serotonin5
has made
possible
studies
of the biosynthesis,
storage
and liberation
of these
pharmacologically
active
stibstances
and
of the growth
of mast
cells
both
in
vivo
and
This
in vitro.
paper
of mast
describes
cells
cell
leukemia
the
circulation.
grown
some
characteristics
in tisstie
in
mice
culture
by
the
of
and
the
introduction
A preliminary
of
report
of
the
tumor,
some
experimental
isolated
these
properties
production
tumor
of mast
mast
experiments
has
cells
into
already
been
Transplants
were
given.6
MATERIALS
Routine
made
transplantation-The
in the
strain
material
fragmented
Preparation
minced
Histamine
and
was obtained
by
volume
of normal
or
urine,
assay
pig
for
in
the
the
)
has
minutes
trypsin
at
the
or
cent
described.5
been
mincing
intrapcriton-eai
per
tumor
intravenous
in
and
tissue
injecting
injection-Fragments
Hank’s
( minus
saline
36#{176}C., centrifuged
at
1,000
clam
of
calciuiii,
rpm
for
in tumor,
from
animals
liver
under
ti.ssue,
deep
blood
ether
iimg-
10
and
minutes
urine--Blood
anesthesia.
An
acid was added
to the sample
of oxalated
sodium
hydroxide
and diluted
with
sea
water
heart,
or Locke’s
solution
for histamine
assay
with
the
medium.
puncture
hydrochloric
tunior
by
determinations
cardiac
METHODS
plasma,
for
on
equal
serum
serotonin
the
guinea
ileum.
A
one
in
acid
using
with
sodium
homogenate
ten
a
glass
plasma
was
added
for
trypsin,
From
partments
17
and
and
the
of
one
to
the
Children’s
Pathology
Supported
by
Submitted
Aug.
M
part
19
18,
assayed
of
as
2.5
solution
was
Cancer
C-2547
tissue
buffer
per
was
made
the
and
of
cent
C.,
37C
tissue
in
normal
supernatant
hydrochloric
was
neutralized
above.
tumor
at
tumor
centrifugation,
phosphate
hours
1958;
or
After
in liver,
and
Grant
liver
grinder.
hydroxide
incubation
of
tissue
Heparin
determination
genized
in 3 parts
of 0.1
the
for
0.25
basal
serotonin
isolated
muscle.
20
Eagle’s
neutralized
on
thigh
in
phosphate)7
resuspended
mice
tissue
stirred
of
( LAF1
the
tumor
were
and
origin
origin
into
of
tumor
nesium
and
of
AND
trypsin
10
N
netitralized
Research
Pharmacology,
plasma-One
pH
and
B-1470
for
To
in
sodiuni
and
from
publication
770
part
this
buffer
hy(IrOxi(le
was
The
Children’s
Oct.
Institutes
23,
of
1958.
was
to
pH
added
assay
Boston,
of
Center,
Mass.
Health.
homooxalated
8.
to
proce(lure
Medical
School,
National
liver
or
phosphate
Medical
the
of
homogenate
assaye(l.
Foundation,
Harvard
accepted
8.
After
destroy
was
Dc-
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MAST
CELL
based
on
used
only
771
STUDIES
ability
the
in
that
of heparin
bovine
to inhibit
fibrinogcn
clot
was
formation,
used
differed
and
instead
of
dog
from
that
previously
fibrinogen.
RESULTS
Character
tency
ctilar
of
period
grafts.
after
the
now
the
strain
( fig.
for
fast-growing
line.
No
of
passages.
the
siderable
growth
cell
with
( fig.
3)
cells
or
sections
are
loaded
marrow,
1 shows
from
mast
give
mast
obtained
normal
two
cells
spinal
as is not
to
was
shape
toluidine
in the
( Blutmastzellen
hemopoietic
in
which
( 1910)
) in
the
of
hemopoietic
had
the
mouse.
cells
tissties
of
obbone
derived
the
mouse
mastocytomas,
characterized
hepatomegaly
nodes.
Lung
nodules
injected
after
the
a latency
presence
of
were
rare.
paralysis
of
were
In
hind
a few
commonly
4b),
Infiltration
of the
mice.
nu-
( fig.
splenomegaly
metastases
catised
leukemias
tumor
by
and
frequently
were
developed,
legs,
cases
formed
the
in
the
tissues.
cells
or blood
prodticed
1.-Latency
TABLE
and
of mice
tumors
Duration
of
with
mast
at the
site
Mast
Cell
cell
of
Tumor
I
II
III
No.ofMice
11
24
12
54
Mean
103
main
line
fast line
main
line
fast line
0Days.
The
animals
here
225
listed
Mean
Range
84-114
169-284
either
Range
86-136
117
126-223
186
died
or
were
sacrificed
leukemia
injection
when
more
Grafts
Original
intramuscular.
cells,
tumor
of
in imprints
mast
Passage
Duration.0
Duration,
the
blue
tissues
whether
A letikemia
lymphatic
Small
Trypsinized
tumor
injected
intramuscularly
Latency.
Latency.
one
con-
stained
of
or an epithelial
is disclosed
or
course
any
been
and
trypsinized
months,
blood,
ganglia
infiltrated.
subcutaneous
three
the
and
uncommon
have
in a preparation
determine
the
mice.
of lymph
cord
must
MaximowTM
in
from
LAF1
in
enlargement
the
the
in
granules.
to
multiply
in
scarce
size
normal
out
in the first few
3 ) even
in the
cells
leukocytes
the
decreased,
basophils.
cells,
of about
in
there
Wright-Giemsa
carried
has
the
characteristic
rare
blood
in
withotit
skull
are
grams
( fig.
were
a monocytoma
of the
cells
Ia-
variants.
mast
of
leukemia-Although
could
to
mast
merotis
cell
been
tumors
rise
Isolated
period
with
the
the
of basophil
cells
intravenously
of
mast
have
solid
and
for either
The
character
that
the
intramustumor
( time
observed
figures
indicated
uniformity
development
Experiments
tumor
stained
was
mitotic
)
apparent
metastasize
the
with
of
the
to
following
of the
growing
attained
size
of 20 to 24
cells
were
well
granulated
Figtire
Note
Production
served
)
the
which
could
be mistaken
low-grade
malignancy.
all
1 presents
to become
to rapidly
Although
division.
passages.-Table
ttimor
rise
tendency
of
hematoxylin.
the
give
to
4a
The
of
a number
repeated
of our mastocytoma
in mice
of passages,
the latency
period
tends
Mastocytomas
passage
generations.
section,
with
required
inoculation
and
tumor
and duration
In the course
IV
79
Mean
Range
Mean
Range
Mean
Range
92
90-114
71
56
34
45-83
24-45
101-216
67
165
96
46-108
49-86
117-232
63-131
89
55
47-135
43-63
large
tumors.
inoculations
were
140
with
All
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772
ONO,
.
1 (at
FIG.
the
2.0
Fir..
or
cm.
grown
3
size
and
shape
( H&E
across.
2 (bottom-,
those
FIG.
of
on
(bottom,
glass
of
350x
left).-Mast
cells.
lUiIT1I
‘
of
the
ANI)
a
mast
cell
tnmior
grafted
thigh.
in
The
tumor
was
well
localiied
and
fluid.
They
are
round,
unlike
those
Note
rneaslmre(l
).
cells
which
IIAUIN
‘1
appearance
top).-Nlicroscopic
uniformity
about
.___
zoMl’I;’irl,
in
are
ascitic
polygonal
riglmt).-Imnprint
of
with
mast
cells
from
a
in
tumimors
1iO()x
( \Vriglit-Gicnisa
processes.
( \\right-Cienisa
niastocytomna.
1250x).
rapidly
the
than
same
from
spleen,
the
leukemias
number
site
kidney,
of cells.
of injection,
stibctitaneous
in
inice
Cells
that
from
pro(lucing
tissues
had
the
been
(lissenlinated
and
injected
intramuscular
bone
intravenously
graft.
tumor
marrow.
nodules
Blood
vith
however,
in
imtst
spl-ead
the
cell
liver,
counts
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MASI’
773
STUDIES
(;ELL
-S
.i--
.,1
I
V.
5-
5-
_
- S
-.
S
Fic.
4 (at
tell
ni;ist
lI(;.
5
(.iemmmsa
\%r(Cr(C
th
C
(at
PS-
S
bottoiii
a grafted
mast
hepaton)egaly
has
and
innumncral)le
tells
of
tuna)r
mammary
).-Smnear
of
cell
tunior
in
splenoniegaly.
mmotltiles
the
Arrow
in
the
lung
right
thigh.
points
Mouse
to
following
b has
paravertebral
intravenous
in-
carcimionia.
blood
froni
a mouse
with
mimast
( Wright-
(dl
leukemia.
iii
establishing
).
(l(\lt((l
This
vitli
1)100(1
Salient
in
with
trvl)sinized
(()()X
tuniors
illustratc(l
a has
top).-Mousc
Niotise
of
5Q
-
‘S
-
-
-
:-
leukemia
infiltration.
j(ctiOfl
C’-.
S
-
S-
1)100(1
in
1)100(1,
remnin(ls
of
findings
figure
lU
5.
contrast
of
Ol1(
vith
niice
are
s-iice
shown
vith
to
tliose
earlier
experiences
lyniphoid
in
tal)le
2,
and
mnast
the
rarely
l1laStOCytOflhits
vith
solid
leukemia.
cell
leukeinic
COlltaille(l
leumkemnia,
in
picture
mast
vhich
is
cells
mast
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774
ONO,
2.-Blood
TABLE
Changes
in
Mice
wit/i
ZOMPETTI,
Mast
Cell
HAGEN
Tumors
and
Leukemia
Mast
Normal
WBC
per
Mean
Range
Mean
Range
11,390
4,050-19,100
49-51
16.030
7,900-35,600
50
cell leukemia
line C
ce 11 leukemia
Range
38
26-44
16-81
1-10
13
37
4
11-62
2-9
7-79
39
7-68
1-11
0
7
Mean
Range
23
34
4
6-72
14-65
2-6
count
100 cells:t
cells
0
Neutrophiles
Lymphocytes.
large
Lymphocytes,
Eosinophiies
Monocytes
small
0
6-13
3-5
78-85
9
4
81
0
6
0
45
0
4
43
1
7
2-8
2-62
31
6-51
0
8
2-12
2-17
cases.
t4-14
cells
Mast
6,150-8,200
53-53
53
Four
tumor
7,110
Hematocrit’
Mast
cell
Mean
(cu.mm.)0
Differential
Mast
FURTH
AND
cases.
averaged
13
Hematocrit
per
values
decreased
in
mast
tensive
diffuse
The
ulated
circulating
than
the
cell
fluid
are
the
main
in
leukemic
cells
mast
appear
cells.
mtich
line
and
cell
tumor-bearing
This
bone
examined
in fluid
and
mast
hosts.
of the
mast
tissue
spherical
in
normal
infiltration
blood
mast
cells are
By way of comparison,
to the
cent
were
23
per
cent
in
subline
mice,
is readily
but
explained
C.
much
by
the
cx-
marrow.
to
A
be
valid
somewhat
comparison
in smears,
and
tisstie
ctiltures
smaller
than
smaller
is
and
difficult
less granbecatise
tumor
mast
cells
in imprints.
the mast
cells
floating
in the
the
polygonal
cells
which
adhere
glass.
Examples
of the microscopic
picture
of the hemopoietic
organs
with
mast
cell leukemia
are seen
in figtire
6 ( spleen
) and figtire
In the liver
there
is a massive
diffuse
intrasinusoidal
accumulation
7
cells
is densely
with
occasional
infiltrated
with
small,
being
bone
marrow
small
neoplastic
encroached
is
tumor-like
mast
upon
diffusely
spinal
injection
neoplasm
infiltration
peritoneal
lining.
Figure
viscera.
diffuse
Hepar-in,
Small
2 shows
histamine,
by
The
the
of the
masses
were
from
ascitic
246
serotonin
levels
pointed
heparin
,
8)
present
no
within
cell
In table
tumors,
held
sign
of
the
3 are given
the
in the livers
and mast
cell leukemia.
kemias
are also included
mast
an
of tumors,
units/gram
or
ascitic
the
blood
out that
the mast
cell
and serotonin.
Tumors
hemorrhage
pulp.
The
infiltration
structtires
on
although
the
cell
mast
cxtype
around
surfaces
of
the
of
the
fluid.
and
8 to 140 i.tg./gram
of serotonin.
quantities
of such
pharmacologically
exhibited
in
mast
and
contained
were
pulp
cells
mice
( liver).
of
preserve(l,
produced
tamine/gram
such
large
rials
mast
and
generations
mals
to 1,540
splenic
are
mesentery
tumor
and
of
cord
and ganglia.
of tumor
cells
a smear
original
description5
it was
large
quantities
of histamine,
The
follicles
mass
( fig.
infiltrated
tends
to the meninges,
The
intraperitoneal
with
nodtiles.
cells.
of
of heparin,
shock,
and
liver.-In
to 4.2
0.85
Despite
the
active
materials
indicating
the
tumors
contained
of the first few
that
mg.
presence
these
these
hisof
animate-
cells.
values
for
of normal
The values
as controls.
histamine,
mice
and
heparin
those
in livers
of mice
In general,
the
and
with
with
liver
serotonin
mast
cell
transplantable
histamine,
in mast
tumors
letiheparin
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MAST
CELL
6 (top,
Fic.
follicle
in
7 (top,
Fic.
8
and
left).-Leukemic
the
Fm.
tension
right
the
is
4 and
5. The
results
tomas,
the
active
are
the
present
urine.
urine
to
values
in
are
the
blood,
of
in
the
by
by
ganglia.
degree
of mast
materials
animals
even
marrow
spinal
active
retained
the spleen.
The
225x).
liver.
( H&E 225x).
bone
the
these
of
with
the
though
lymphoid
( H&E
infiltrating
parallel
that
pulp
size.
vertebral
and
of
indicate
materials
the
bone
appear
content
and
the
the
in
infiltration
of
along
filling
reduced
leukemic
infiltration
plasma
in
quadrant
bottom).-Replacement
serotonin
(luantities
creted
infiltration
tipper
right).-Massive
(at
of
The
775
STUDIES
are
large
mast
mast
cell
given
localized
cells,
significant
cells
( H&E
and
amounts
and
ex-
135x).
infiltration.
in
tables
mastocyonly
small
are
cx-
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776
ONO,
3.-Histamine,
TABLE
Heparin
Histamine
Types
of
Neoplasms
No. of
cases
Mean
5
2S9
Mastocytoma5
Liver
and
equiv.
Serotonin
zOMPEm,
Content
y/g.
of
Heparin
HAGEN
Mastocytomas
and
unts/g.
FURTH
Licers
Scrotonin
Range
No. of
cases
No. of
cases
Mean
Range
850-4200
6
664
247-1540
4
/g.
Mean
itange
326
8-714
of hosts:
Normal
Mastocytoma
Mast
cell leukemia
7
0.07
0.01-0.1
6
9.5
5.8-12.0
2
13
8
1.56
304.0
0.07-7.8
30-560
6
14.8
4.68-46.0
2
7
246
Mast cell leukemia
(line
C)
8
395.0
56-920
7
32
6.2-105
Viral
leukemiat
Reticulum
sarcoma
4
1
0.5-0.5
3
1
7.8
iO.5
6.0-10.0
Each
assay
of
Some
of these
tVirus
of
0.5
0.2
the same
kind
was made
values
were
included
in
Friend,
causing
reticulum
cell
No. of
cases
mice
Normal
Mast
cell
Mastcell
Friend
tumor-bearing
leukemia
leukemc
See
footnote
to
Serum
values.
table
a different
preliminary
188-372
equiv.
and
Serotonin
‘y/ml.
Values
Heparin
No.
Mean
Range
cases
Mean
0.4
0.04-0.73
4
3.3
5
0.74
0.3-1.62
4
6
4
1.3
0.5
0.6-2.9
0.5-0.5
7
In
Mast
cell
tumor
Mast
cell
leukemic
animals
eight
that
TABLE
5.-Histamine
bearing
table
with
2.1-4.9
4.31
out
Mast
mast
on
trypsinized
grown
( figs.
9 and
when
injected
10
Apparently
2.1-2.3
1
7.74
7.74
in
Urine
equiv.
y/ml.
Range
0.91
0.5(3-1.25
46.0
18.3-74.9
359.0
184.()-493.0
plasma
histamine
histamine
bearing
release
and
levels
mast
their
cell
active
of raised
proteins.
heparin
on
are
tumors.
materials.
values
the
average
This
suggests
The
lack
plasma
heparin
valtles
The
heparin
assays
were
of
may
car-
plasma.
cells
the
using
growth
The
cells
).
animals
blood
tLsue
mice
presence
the
Range
2.2
Mean
urinary
in the presence
of heparin
with
of 138
the
in
the
in
into
a latency
show
Mean
0.35-9.5
Values
leukemia,
the
than
in
celLs
dium
with
cell
and
cells
cell tumor
supported
-/ml.
2
17.5-288
4
mast
a hemorrhagic
state
be due to a binding
ned
No. of
cases
3.
greater
mast
<0.2
Serotonin5
101.3
4
elevated,
times
the
I
10
3.1-i
Plamia
Range
2
greatly
56.5
0.2-2.8
3.
Host
are
2
units/nil.
No. of
cases
to
of
of
5
Normal
footnote
<0.17
1.5
animal.
report.
Histamine
See
<0.17
sarcoma.
Heparin
Histamine
Host
on
the
4.-Histamine,
TABLE
not
AND
basophilic
culture.-Tissue
retained
35
days
of
ctiltures
Eagle’s
meditim.
of mast
cells
days
their
after
Without
with
the
capacity
growth
developed.
to
in
However,
histamine
in
granules
are
the
only
grow
vitro,
assays
tissue
culttire
a matrix,
prepared
were
supplements,
formation
and
typical
of tissue
fluid
probably
from
this megranules
of
form
ttimors;
mastocytomas
culttires
at
this
of
did
time.
protein,
From www.bloodjournal.org by guest on June 18, 2017. For personal use only.
MAST
CELL
777
STUDIES
;
. .- :.
-
.
S #{149} ‘‘1’$I
-.
‘I
‘-S
.
-
:t
.‘?
,,Y
.
4
-‘
.
..‘
S
S
(at
9
FIG.
Gienisa.
top).-Mast
The
Fir..
10
nummnt-romms
cell
(at
in
cells
the
center
.
-
.‘C:’
‘-
on
niast
tli-
and
glass
in
---
liquid
.5-.
medium
stained
with
Wright-
( 625x).
is a fibroblast.
/)ottOlfl).-UmThtaiIlc(I
I)semRlol)odia
\-:
grown
S
r.
.
,
S.-
.
,.
1
cells
gro\vn
char;m(teristie
on
granularity
in
glass
of
the
liquid
cells.
medium.
(
Phase
Note
the
contrast;
600).
for
storage
l)eri11e13ts
ing
the
of
to
level
the
deterniine
of
Schindler,
Day
iiitl
niaocyoni10
Potter
pharmiacologicallv
afl(l
time
active
of histamnine
the
forniation
corresponding
Fischer”
have
coiiipounds
amino
shovn
aci(ls
the
al)ility
to 1)ro(l mice serotonin
in
when
fornied.
Ex-
and serotonin
by increasare
in progress.
Recently
of mast
cells of the Dunn
tissue
culture.
From www.bloodjournal.org by guest on June 18, 2017. For personal use only.
778
ONO,
zoMpErrI,
HAGEN
AND
FURTH
DiscussioN
Two
mated
transplantable
strains
in mice
as solid
ttimors
described
That
that
by
by
isolated
functional,
Dunn
us3
known
are
does
as,
or other
reported
serotonin
Potter1#{176} is
slowly,
as much
tumor
Experiments
and
grows
producing
as any
of mast
cell neoplasm
and are transplantable
or
invasive
not
in
and
more,
grows
relatively
readily
heparin,
histamine
cell
that
granules
heparin,
which
histidine
decarboxylase
histamine
from
thesize
the
heparin
Mast
cell
tissue
mast
in vitro
leukemia
cells
has
ments
indicate
types
of
and
their
leukemias
has
that
functions
ing
the
mast
cells
with
usual
blood
injection
It was
cells
cells
of
essential
identity.
and
mice
the
character
have
been
with
and
basophilic
that
after
specific
store
when
small
numbers.
and
and
to mast
in
needed?
to be
connective
tissue
heparin
What
are
and
A highly
functional,
solitary
des
subcutaneous
and
Mast
cell
slowly
leukemia
it
growing
relation
lead
between
to sup-
us
type
possessing
special
occasionally
enter-
characterized
By subcutaneous
The
known
storage
in
at
time,
that
unknown.
is
believed
the
active
in premyelocytes
determined.
an
independent
the
These
cell
system
capacity
substances
to be explored
What
causes
with
to
synthesize
are
stored
a
in
are:
What
their
release
released?
CONCLUSIONS
neoplasm
tumors
when
so
may
of
localized
mast
at the
cells
site
is
of graft
described.
in mus-
tissues.
results
by
mast
They
that
or absence
when
transplantable
diverse
hemopoietic
and
remains
presence
serotonin.
AND
cells
to
experi-
with
the
was
cells.
not
possessing
functions
SUMMARY
It causes
in
leukemia.
The basic
problems
of these
substances?
their
and
syn-
also
localized
tumors
were
produced.
host
resistance
by irradiation
the
was
as constituting
specific
granules.
the
production
cell
granuilocytes
type.
Although
remains
histamine,
in
of
produce
their
serotonin
cells
of studies
tissues
serotonin
cells,
from
blood
mast
The
present
changes
myeloid
leukemia”1
of the mveloid
granulocytes,
reduction
conceived
habitat
mast
of
decades
of
blood
connective
spread
granules
are
two
in
those
basophilic
the
premyelocyte
cells
the cell
regulates
very
to
That
and
separable
synthesizing
relation
conjectural.
studies
independent
histamine
basophile
primary
an
serotonin
in a sucrose
decarboxylase
has indicated
a developmental
organs
of mice.
Present
sttidies
of
are
Mast
are
in
tended
of
materials
one
and
demonstrated.
In
in
transplantable
in the cytoplasm
heparin,
of
acids.’2”3
been
of
stream
of these
also noted
ttinIor
recently
residence
The
first reported
basophilic
granules
capable
amino
is rare
in man.15
The
thus
far been
largely
system
of the rodent
no
mast
cells
and
hemopoietic
pose
are
corresponding
is highly
histamine
are
mitochondria
and other
cytoplasmic
particles
by centrifugation
density
gradient.
The mast
cells contain
5-hydroxytryptophane
and
fast;
and
tissue.
indicate
mast
known.”#{176} Both
origin the strain
of origin.
metastasize
mammalian
elsewhere”
contained
are
isolated
cells
are
injected
intravenously.
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MAST
CELL
The
blood
of mice
injected
with
These
and
other
mast
cell
primary
and
produces
the
residence
mast
that
state.
The
liver
solid
otitside
mastocytomas
of infiltrating
mast
when
describite
tumores
tin
de
histos
lente
the
an
hemopoietic
plasma
of
blood
and
cell
type
in
urine.
great
raised
and
independent
organs.
levels
present
in the
are
slightly
S
be slightly
raised
in
in mast
cell leukemias
heparin
values
appear
mice
with
without
to
be
mast
cell
a hemorrhagic
related
to
the
tu-
num-
cells.
SUMMARIO
Es
identity
are
and
serotonin
are
and partly
excreted
histamine
and
essential
cells
histamine
is released
letlkemias,
histamine
the
mast
in tissties
heparin,
histamine
cell
suggest
suggest
tirine
levels
are highly
elevated.
Plasma
heparin
values
may
mors
and are greatly
increased
ber
leukemia
observations
cells
In mastocytomas,
quantities.
Some
In
mast
intramuscularly.
tissue
with
779
STUDIES
IN
INTERLINGUA
ftinctionalissime
crescentia
neoplasma
que
es
transplantabile.
localisate
al sito
del
Illo
graffo
in
causa
musculo
e
suhcutanee.
Leucemia
venose.
mastocytic
Le
cytomas
sanguine
(ltm1tn(1o
Iste
e
(Ic
con
per
via
observationes
es
dente
quando
muses
injicite
altere
mastocytos
resulta
de
cellulas
identic
que
isolate
es injicite
mastocytic
procltice
p1’
via
intra-
solide
masto-
intramuscular.
pare
essentialmente
cellulas
leucemia
reside
indicar
que
e que
hematomastocytos
mastocyots
primarimemite
in
es
histos
e
tin
typo
foras
histo-
indepen-
(id
organos
liematopoietic.
Craiide
quantitates
mastocytomas.
(Ic
Un
certe
in Ic urina.
In Ic leucemias
mente
In le urina,
valores
pro
tumores
absentia
Le
heparina
mastocytic
de
un
valores
stato
de
al numero
del
histamina,
e
(IC histalnina
mastocytic,
elevate.
Le
con
heparina,
amonta
Ic nivellos
es grandemente
del
plasma
e grandemente
(s
1)res’nte
e excernite
histamina
de
illos
serotonina
es liberate
in
le plisma
in
in
parte
es
love-
elevate.
pote
esser
elevate
levemente
elevate
in leimcemias
in
muses
mastocytic
in
le
hemorrhagic.
histamina
infiltrate
c heparina
hepatic
es
apparentemente
relationate
mastocytos.
REFERENCES
1.
J.:
Padawer,
mast
cells.
Trans.
19:690,
Sc.
2.
Studies
New
mammalian
York
4.
Acad.
mechanism
J.Pharm.&
Riley,
of
7:267,
Methodologische
Physiologic
Verschiedenen
of
histamine
Pharn3acol.
10:1,
1958.
3.
P. :
Ehrlich,
zur
1957.
Uvn#{228}s, B. : The
Iil)eration.
on
ten.
5.
Furth,
und
Formen
Z.Klin.Med.
J.,
J. F.:
Pharmacology
mast
cells.
1955.
and
Pharmacol.
functions
Rev.
mouse
and
der
1:553,
Hagen,
P.
Transplantable
the
Beitr#{228}ge
Pathologic
1880.
and
niastocytoma
containing
histamine,
5-hydroxytryptamiiine.
Exper.Biol.&
Med.
der
Leukocy-
95:824,
Hirsch,
E.:
in
heparin,
Proc.Soc.
1957.
the
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ONO,
Ono,
S.,
Zompetti,
Hagen,
P.:
cells
to
to
7.
T.
T.,
J.:
onies
single
der
III.
S#{228}ugetiere.
76:63,
1910.
R.,
Culture
their
Die
and
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Em-
M.
of neoplastic
5-hydroxytryptamine
content
1958.
in
vitro.
and
Entste-
L.,
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M.:
A
trans-
neoplasn4
Nat.Cancer
in
Inst.
Hagen,
P.
and
the
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as
H.
and
J.:
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heparin-containing
distinct
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Graham,
Hagen,
P.
Koon,
in
fromu
18:387,
T. :
and
mitoclion-
1958.
Personal
Lee,
communica-
E.
D.
cells
and
Fed.Proc.
mast
16.
Forth,
kemia
1935.
L. : Aniino
inouse
143:7P,
1958.
D. : The
synthesis
mouse
niast
Friedman,
F.
of
Am.Chem.Soc.
Fischer,
mast
F.
J.Phy5iol.
14.
Arch.milcr.Anat.
S.
Potter,
cell
(lecarbOxylases
15.
Day,
.
AND
tion.
Knochen-
Die
Lee,
granules
#{252}ber
des
and
mast
J
dna.
13.
Mastzellen.
Schindler,
histamine
in
J.Exper.Med.
Untersuchungen
B.
Histaniinecol-
with
HAGEN
1957.
Cieciura,
cells
cells
layer.
Histogenese
A.:
Am.J
of
Bindegewebe.
der
17:409,
Hela
A. :
und
and
mammalian
T.
mouse.
1956.
bryonale
G.
and
I.
characteristics
NIaximow,
hung
masto-
leukemia.
P.
a “feeder”
marks
of
Dunn,
plantable
11.
Marcus,
from
10.
mast
1958.
of
103:273,
9.
cell
and
blood
cells,
growth
without
Blut
of
Growth
citro;
8.
mast
.34:567,
Puck,
S.
mast
J.
Furth,
relation
tissue
cytoma
Path.
L.,
The
ZOMPETrI,
cell
of
tumor
80:1520,
B.
I.,
Will,
mice.
heparin
J.
slices.
1958.
J. J.,
G. and
Braunstein,
cell leukemia.
Blood
J. : Transmission
of
in
aci(l
cells.
mii:ust
J.Exper.Med.
Freiman,
H. :
13:70,
myeloid
Tissue
1958.
len61:423,
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1959 14: 770-780
Relation of Mastocytoma to Mast Cell Leukemia, and of Heparin, Histamine
and Serotonin to Mast Cells
SABURO ONO, LENA ZOMPETTI, PAUL HAGEN and JACOB FURTH
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