LIBRARY PREPARATION – DNA
DNA CLONING
DNA AMPLIFICATION & PCR
NEBNext for Illumina
DNA Product Selection Chart
®
®
Use the following chart to determine the best NEBNext products for your
Illumina DNA library prep needs.
Fragmentase
RNA ANALYSIS
LIBRARY PREP FOR NEXT GEN SEQUENCING
PROTEIN EXPRESSION & ANALYSIS
CELLULAR ANALYSIS
NEBNext dsDNA Fragmentase – #M0348
Kit
NEBNext Ultra II DNA Library Prep Kit – #E7645*
Oligos
Fast,
low input
EPIGENETICS
NEBNext Multiplex Oligos – #E7335/#E7500/#E7535/#E7600/#E6609**
NEBNext dsDNA Fragmentase – #M0348
NEBNext Ultra II End Repair/dA-Tailing Module – #E7546*
Modules
NEBNext Ultra II Ligation Module – #E7595*
NEBNext Ultra II Q5 Master Mix – #M0544*
Oligos
Genomic
Fragmentase
NEBNext dsDNA Fragmentase – #M0348
Kit
NEBNext DNA Library Prep Master Mix Set – #E6040***
Oligos
Standard
NEBNext Multiplex Oligos – #E7335/#E7500/#E7535/#E7600/#E6609**
NEBNext Multiplex Oligos – #E7335/#E7500/#E7535/#E7600/#E6609**
NEBNext dsDNA Fragmentase – #M0348
NEBNext End Repair Module – #E6050
Modules
NEBNext dA-Tailing Module – #E6053
NEBNext Quick Ligation Module – #E6056
NEBNext Ultra II Q5 Master Mix – #M0544
DNA
Oligos
Kit
NEBNext Ultra II DNA Library Prep Kit – #E7645*
Oligos
Fast,
low input
NEBNext Multiplex Oligos – #E7335/#E7500/#E7535/#E7600/#E6609**
NEBNext Multiplex Oligos – #E7335/#E7500/#E7600/#E6609**
NEBNext Ultra II End Repair/dA-Tailing Module – #E7546*
Modules
NEBNext Ultra II Ligation Module – #E7595*
NEBNext Ultra II Q5 Master Mix – #M0544*
Oligos
ChIP
Kit
NEBNext ChIP-Seq Library Prep Master Mix Set – #E6240**
Oligos
Standard
NEBNext Multiplex Oligos – #E7335/#E7500/#E7600/#E6609**
NEBNext Multiplex Oligos – #E7335/#E7500/#E7600/#E6609**
NEBNext End Repair Module – #E6050
NEBNext dA-Tailing Module – #E6053
Modules
NEBNext Quick Ligation Module – #E6056
NEBNext Ultra II Q5 Master Mix – #M0544*
Oligos
* Reagents for the original Ultra workflow are also available. See ordering information.
** Singleplex Oligos are also available. See ordering information.
*** Set of individual reagents is also available. See ordering information.
Ill DNA_Sel_MS
3/16
NEBNext Multiplex Oligos – #E7335/#E7500/#E7600/#E6609**
LIBRARY PREPARATION – RNA
DNA CLONING
DNA AMPLIFICATION & PCR
NEBNext for Illumina
RNA Product Selection Chart
EPIGENETICS
Use the following chart to determine the best NEBNext products for your
Illumina RNA sequencing needs.
PROTEIN EXPRESSION & ANALYSIS
®
Small RNA
®
RNA ANALYSIS
LIBRARY PREP FOR NEXT GEN SEQUENCING
CELLULAR ANALYSIS
NEBNext Multiplex Small RNA Library Prep Set (Set 1/Set 2) – #E7300/#E7580
NEBNext Small RNA Library Prep Set (Multiplex Compatible) – #E7330
NEBNext Ultra™ Directional RNA Library Prep Kit – #E7420
Kit
NEBNext rRNA Depletion Kit – #E6310
NEBNext Poly(A) mRNA Magnetic Isolation Module – #E7490
Oligos
NEBNext Multiplex Oligos – #E7335/#E7500/#E7600/#E6609*
NEBNext rRNA Depletion Kit – #E6310
Directional
Streamlined
NEBNext Poly(A) mRNA Magnetic Isolation Module – #E7490
NEBNext RNA First Strand Synthesis Module – #E7525
Modules
NEBNext Ultra Directional RNA Second Strand Synthesis Module – #E7550
NEBNext Ultra End Repair/dA-Tailing Module – #E7442
RNA
NEBNext Ultra Ligation Module – #E7445
Fast,
low input
NEBNext Q5 Hot Start HiFi PCR Master Mix – #M0543
Oligos
NEBNext Multiplex Oligos – #E7335/#E7500/#E7600/#E6609*
NEBNext Ultra RNA Library Prep Kit – #E7530
NEBNext rRNA Depletion Kit – #E6310
Kit
NEBNext Poly(A) mRNA Magnetic Isolation Module – #E7490
Oligos
Non-directional
Streamlined
Total RNA
mRNA
NEBNext Multiplex Oligos – #E7335/#E7500/#E7600/#E6609*
NEBNext rRNA Depletion Kit – #E6310
NEBNext Poly(A) mRNA Magnetic Isolation Module – #E7490
NEBNext RNA First Strand Synthesis Module – #E7525
Modules
NEBNext mRNA Second Strand Synthesis Module – #E6111
NEBNext Ultra End Repair/dA-Tailing Module – #E7442
NEBNext Ultra Ligation Module – #E7445
NEBNext Q5 Hot Start HiFi PCR Master Mix – #M0543
Oligos
Kit
NEBNext Multiplex Oligos – #E7335/#E7500/#E7600/#E6609*
NEBNext mRNA Library Prep Master Mix – #E6110**
NEBNext rRNA Depletion Kit – #E6310
NEBNext Poly(A) mRNA Magnetic Isolation Module – #E7490
Oligos
NEBNext Multiplex Oligos – #E7335/#E7500/#E7600/#E6609
NEBNext rRNA Depletion Kit – #E6310
Standard
NEBNext Poly(A) mRNA Magnetic Isolation Module – #E7490
NEBNext Magnesium RNA Fragmentation Module – #E6150
NEBNext RNase III RNA Fragmentation Module – #E6146
Modules
NEBNext RNA First Strand Synthesis Module – #E7525
NEBNext mRNA Second Strand Synthesis Module – #E6111
NEBNext End Repair Module – #E6050
NEBNext dA-Tailing Module – #E6053
NEBNext Quick Ligation Module – #E6056
NEBNext Q5 Hot Start HiFi PCR Master Mix – #M0543
Oligos
*Singleplex Oligos also available. See ordering information.
**Set of individual reagents is also available. See ordering information.
Illumina RNA_Sel_Chart_MS
3/16
NEBNext Multiplex Oligos (Index Primers Set 1/Set 2) – #E7335/#E7500/#E7600/#E6609*