Figure S1. NH4Cl treatment does not influence the anti-apoptotic effect of CpGODN. Annexin V-FITC and PI binding were measured by flow cytometry in INA-6
cells treated for 48 hours in RPMI with 10% FCS and 1ng/ml IL-6 with BMP-6 (100
ng/ml) and CpG-ODN (4uM) as indicated. Percentages of viable non stained cells are
presented. Error bars represent 1 SD of duplicate measurements.
Figure S2. CpG-ODN does not inhibit Smad-signaling in HEK cells. Levels of
phosphorylated Smads 1/5/8 in total cell extracts from HEK 293-ELAM-luc (HEK)
cells and HEK TLR9-293-ELAM-luc (HEK-TLR9) cells treated as indicated with
BMP-2 (300 ng/ml) and CpG-ODN (4μM) for 30 minutes.
1
Block Type 96well
Chemistry SYBR_GREEN
ExperimentD:\Applied Biosystems\StepOne Software v2.1\experiments\Nikolai\Results\diff dag 1 supera
Experiment2008-07-04 12:03:26 PM CEST
Instrument steponeplus
Passive ReROX
Well
A1
A2
A3
A4
A5
A6
A7
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
Sample Na Sample Co Biogroup NBiogroup CTarget NamTarget ColoTask
Reporter
Target 1 RGB(139,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 2 RGB(208,2UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 3 RGB(244,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 4 RGB(255,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 5 RGB(243,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 6 RGB(192,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 7 RGB(249,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 8 RGB(253,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 9 RGB(139,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 10 RGB(208,2UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 11 RGB(244,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 12 RGB(255,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 13 RGB(243,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 14 RGB(192,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 15 RGB(249,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 16 RGB(253,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 17 RGB(139,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 18 RGB(208,2UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 19 RGB(244,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 20 RGB(255,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 21 RGB(243,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 22 RGB(192,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 23 RGB(249,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 24 RGB(253,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 25 RGB(139,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 26 RGB(208,2UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 27 RGB(244,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 28 RGB(255,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 29 RGB(243,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 30 RGB(192,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 31 RGB(249,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 32 RGB(253,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 33 RGB(139,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 34 RGB(208,2UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 35 RGB(244,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 36 RGB(255,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 37 RGB(243,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 38 RGB(192,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 39 RGB(249,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 40 RGB(253,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 41 RGB(139,1UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 42 RGB(208,2UNKNOWNSYBR
diff dag 1 RGB(132,1
Target 43 RGB(244,1UNKNOWNSYBR
diff dag 1 RGB(132,1
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
diff dag 1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
RGB(132,1
Target 44
Target 45
Target 46
Target 47
Target 48
Target 49
Target 50
Target 51
Target 52
Target 53
Target 54
Target 55
Target 56
Target 57
Target 58
Target 59
Target 60
Target 61
Target 62
Target 63
Target 64
Target 65
Target 66
Target 67
Target 68
Target 69
Target 70
Target 71
Target 72
Target 73
Target 74
Target 75
Target 76
Target 77
Target 78
Target 79
Target 80
Target 81
Target 82
Target 83
Target 84
Target 85
Target 86
Target 87
Target 88
Target 89
Target 90
Target 91
Target 92
Target 93
Target 94
RGB(255,1UNKNOWNSYBR
RGB(243,1UNKNOWNSYBR
RGB(192,1UNKNOWNSYBR
RGB(249,1UNKNOWNSYBR
RGB(253,1UNKNOWNSYBR
RGB(139,1UNKNOWNSYBR
RGB(208,2UNKNOWNSYBR
RGB(244,1UNKNOWNSYBR
RGB(255,1UNKNOWNSYBR
RGB(243,1UNKNOWNSYBR
RGB(192,1UNKNOWNSYBR
RGB(249,1UNKNOWNSYBR
RGB(253,1UNKNOWNSYBR
RGB(139,1UNKNOWNSYBR
RGB(208,2UNKNOWNSYBR
RGB(244,1UNKNOWNSYBR
RGB(255,1UNKNOWNSYBR
RGB(243,1UNKNOWNSYBR
RGB(192,1UNKNOWNSYBR
RGB(249,1UNKNOWNSYBR
RGB(253,1UNKNOWNSYBR
RGB(139,1UNKNOWNSYBR
RGB(208,2UNKNOWNSYBR
RGB(244,1UNKNOWNSYBR
RGB(255,1UNKNOWNSYBR
RGB(243,1UNKNOWNSYBR
RGB(192,1UNKNOWNSYBR
RGB(249,1UNKNOWNSYBR
RGB(253,1UNKNOWNSYBR
RGB(139,1UNKNOWNSYBR
RGB(208,2UNKNOWNSYBR
RGB(244,1UNKNOWNSYBR
RGB(255,1UNKNOWNSYBR
RGB(243,1UNKNOWNSYBR
RGB(192,1UNKNOWNSYBR
RGB(249,1UNKNOWNSYBR
RGB(253,1UNKNOWNSYBR
RGB(139,1UNKNOWNSYBR
RGB(208,2UNKNOWNSYBR
RGB(244,1UNKNOWNSYBR
RGB(255,1UNKNOWNSYBR
RGB(243,1UNKNOWNSYBR
RGB(192,1UNKNOWNSYBR
RGB(249,1UNKNOWNSYBR
RGB(253,1UNKNOWNSYBR
RGB(139,1UNKNOWNSYBR
RGB(208,2UNKNOWNSYBR
RGB(244,1UNKNOWNSYBR
RGB(255,1UNKNOWNSYBR
RGB(243,1UNKNOWNSYBR
RGB(192,1UNKNOWNSYBR
H11
H12
diff dag 1 RGB(132,1
diff dag 1 RGB(132,1
Target 95 RGB(249,1UNKNOWNSYBR
Target 96 RGB(253,1UNKNOWNSYBR
array bmp signalling pathway.eds
Quencher Comments
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
None
Results TGFbeta/BMP PCR Array Day 1
Well
A1
A2
A3
A4
A5
A6
A7
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
Sample Name
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Target name
ACVR1
ACVR2A
ACVRL1
AMH
AMHR2
BAMBI
BGLAP
BMP1
BMP2
BMP3
BMP4
BMP5
BMP6
BMP7
BMPER
BMPR1A
BMPR1B
BMPR2
CDC25A
CDKN1A
CDKN2B
CER1
CHRD
COL1A1
COL1A2
COL3A1
CST3
DLX2
ENG
EVI1
FKBP1B
FOS
Cт
26.90968132
28.92985916
29.91033554
36.93052292
37.09019852
25.92194939
30.91600037
24.91069794
35.95777512
37.08849335
30.90862274
Undetermined
29.92094803
Undetermined
29.90146446
30.91374779
32.9234314
28.92376518
32.91643143
24.91507912
28.90600014
37.08254623
32.9118576
19.90989876
17.91374397
21.93249321
22.9133606
30.91264725
23.91657257
29.92687988
30.90695572
30.92012215
ΔCт Mean
Ct Threshold
3.998828888
3.176115256
6.019006729
2.053926508
6.999483109
3.349820697
14.01967049
2.176589585
14.17934608
0.113045546
3.011096954
1.930018683
8.005147934
3.552605924
1.999845505
2.985632765
13.04692268
1.530735869
14.17764091
0.226612891
7.997770309
2.047800799
0.2
7.010095596
2.777601827
0.2
6.99061203
3.42964766
8.002895355
3.14065222
10.01257896
2.967865901
6.01291275
2.174038544
10.00557899
3.586613481
2.004226685
3.549786745
5.995147705
3.478201781
14.1716938
0.110431019
10.00100517
3.748689715
-3.00095367
3.074449464
-4.99710846
3.291820434
-0.97835922
2.382926286
0.002508163
2.782062342
8.001794815
2.403431496
1.005720139
2.844632521
7.016027451
2.827246919
7.996103287
3.853549951
8.009269714
4.874371042
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
FST
GDF2
GDF3
GDF5
GDF6
GDF7
GSC
HIPK2
ID1
ID2
IGF1
IGFBP3
IL6
INHA
INHBA
INHBB
ITGB5
ITGB7
JUN
JUNB
LEFTY1
LTBP1
LTBP2
LTBP4
MYC
NBL1
NODAL
NOG
NR0B1
PDGFB
PLAU
RUNX1
SERPINE1
SMAD1
SMAD2
SMAD3
SMAD4
25.91179276
Undetermined
Undetermined
28.90472031
31.91516876
36.91499329
36.90210342
28.91772652
26.92204285
26.91555595
34.90505981
22.91168594
27.91830254
30.89515495
25.90316391
32.89878845
23.91709518
33.9145813
29.91417122
28.91179466
34.90570068
23.90476418
23.92345047
27.92534637
30.91792107
26.91737747
33.91858292
28.91297913
Undetermined
37.13092804
26.91221619
28.92266655
22.91181564
29.91586304
28.92372131
26.90692139
28.90739632
3.000940323
5.993867874
9.00431633
14.00414085
13.99125099
6.006874084
4.011190414
4.004703522
11.99420738
0.000833511
5.007450104
7.984302521
2.992311478
9.98793602
1.006242752
11.00372887
7.003318787
6.00094223
11.99484825
0.993911743
1.012598038
5.014493942
8.007068634
4.00652504
11.00773048
6.002126694
14.22007561
4.001363754
6.011814117
0.000963211
7.005010605
6.012868881
3.996068954
5.996543884
3.281093019
0.2
0.2
2.995981816
2.965819572
2.721578901
3.85683661
3.465765153
3.17255579
2.897826045
2.066629963
4.408523062
2.699065745
3.153895919
4.03028356
1.922035507
5.165915874
2.718848723
3.728223313
4.389678542
3.032454821
2.373809871
3.726417762
2.668182844
2.815450981
4.013934651
2.386343492
2.761699657
0.2
0.221884899
3.698817868
4.985245629
3.966357439
3.789439727
2.492259377
2.411520517
1.544008184
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Untreated Day 1
Well
A1
A2
A3
A4
A5
A6
A7
A8
Sample Name
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
SMAD5
SMURF1
SOX4
STAT1
TGFB1
TGFB1I1
TSC22D1
TGFB2
TGFB3
TGFBI
TGFBR1
TGFBR2
TGFBR3
TGFBRAP1
TGIF1
B2M
HPRT1
RPL13A
GAPDH
ACTB
HGDC
RTC
RTC
RTC
PPC
PPC
PPC
Target name
ACVR1
ACVR2A
ACVRL1
AMH
AMHR2
BAMBI
BGLAP
BMP1
28.91734123
29.91036797
30.91592598
26.91628075
24.90494919
27.91897392
26.92902946
26.90179825
29.91448212
20.90619278
28.90450668
35.95287323
27.91184425
27.91841507
27.91428757
22.9256916
27.91573143
24.92050743
20.91269684
20.91451836
Undetermined
37.00564957
Undetermined
37.13140106
22.90882683
22.91085243
22.91629028
Cт
27.91022491
30.94096184
31.92284203
37.00554657
37.18777847
26.92633438
31.91384506
25.9144516
6.0064888
6.999515533
8.005073547
4.005428314
1.994096756
5.00812149
4.018177032
3.990945816
7.003629684
-2.00465965
5.993654251
13.0420208
5.000991821
5.007562637
5.003435135
0.014839172
5.004878998
2.009654999
-1.99815559
-1.99633408
14.09479713
14.22054863
-0.0020256
0.005437851
2.407843164
3.429161911
3.544100173
2.778516897
3.827049951
3.432840014
3.925877085
2.932452918
4.609105952
3.763836581
3.128436781
2.818178207
3.128666496
2.518461375
3.686570025
3.334425623
1.20187263
3.159124628
3.47877559
2.23497514
0.2
1.101050079
0.2
0.037027139
3.142495012
2.933734501
2.739568475
ΔCт Mean
Ct Threshold
4.998634338
2.437433429
8.029371262
2.776514364
9.01125145
4.33145524
14.09395599
1.144350789
14.2761879
0.007504132
4.014743805
1.81688367
9.002254486
3.207885662
3.002861023
2.523719954
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
D9
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
BMP2
BMP3
BMP4
BMP5
BMP6
BMP7
BMPER
BMPR1A
BMPR1B
BMPR2
CDC25A
CDKN1A
CDKN2B
CER1
CHRD
COL1A1
COL1A2
COL3A1
CST3
DLX2
ENG
EVI1
FKBP1B
FOS
FST
GDF2
GDF3
GDF5
GDF6
GDF7
GSC
HIPK2
ID1
ID2
IGF1
IGFBP3
IL6
36.92130661
37.16552734
32.90956879
Undetermined
30.91786003
Undetermined
30.91663742
31.90870285
32.92075729
29.92243004
34.92025375
25.91619682
29.90986633
Undetermined
33.90796661
20.90624237
18.91563988
23.93951416
24.92513275
31.91058159
25.92477417
30.92588806
31.90098953
31.90912056
26.9156723
Undetermined
Undetermined
30.90577888
33.92630768
37.03856659
36.93514252
29.9277668
27.91579247
27.9086895
36.9305954
23.90241623
29.91772652
14.00971603
14.25393677
9.99797821
8.006269455
8.005046844
8.997112274
10.00916672
7.010839462
12.00866318
3.004606247
6.998275757
10.99637604
-2.00534821
-3.9959507
1.027923584
2.013542175
8.998991013
3.013183594
8.014297485
8.989398956
8.997529984
4.004081726
7.994188309
11.0147171
14.12697601
14.02355194
7.016176224
5.004201889
4.997098923
14.01900482
0.990825653
7.006135941
2.270505296
0.00811429
2.751176904
0.2
2.976527182
0.2
2.189873949
3.046680895
2.222877772
1.957597355
2.217590083
3.475636098
3.708447648
0.2
3.660827561
3.366774843
2.437298442
2.84215775
3.96325518
2.659182394
3.980642694
2.5451502
3.904317326
3.78526804
3.011453524
0.2
0.2
2.771599431
3.469975589
0.902492343
2.388877989
3.479366564
2.916132689
3.16933884
1.294040904
3.454548711
3.702324646
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
INHA
INHBA
INHBB
ITGB5
ITGB7
JUN
JUNB
LEFTY1
LTBP1
LTBP2
LTBP4
MYC
NBL1
NODAL
NOG
NR0B1
PDGFB
PLAU
RUNX1
SERPINE1
SMAD1
SMAD2
SMAD3
SMAD4
SMAD5
SMURF1
SOX4
STAT1
TGFB1
TGFB1I1
TSC22D1
TGFB2
TGFB3
TGFBI
TGFBR1
TGFBR2
TGFBR3
31.89858437
26.90705872
34.90335083
24.90810204
35.91736603
30.91910934
29.91003799
36.97359467
25.90382767
24.91983414
28.92708397
32.91993713
27.91544151
34.91477966
29.91930962
37.04909897
Undetermined
27.90202332
29.91644859
23.90482903
30.90433693
29.92373657
27.90646744
30.91163445
29.92250824
30.89731979
31.91419411
28.91069984
26.91532707
28.92695808
27.91086197
28.90239716
30.90490532
22.90951347
29.90756226
36.979702
29.91254234
8.98699379
3.99546814
11.99176025
1.996511459
13.00577545
8.007518768
6.998447418
14.06200409
2.992237091
2.008243561
6.015493393
10.00834656
5.003850937
12.00318909
7.00771904
14.13750839
4.990432739
7.004858017
0.993238449
7.992746353
7.012145996
4.994876862
8.000043869
7.010917664
7.985729218
9.002603531
5.999109268
4.003736496
6.015367508
4.999271393
5.99080658
7.993314743
-0.0020771
6.99597168
14.06811142
7.000951767
2.690088873
4.152752834
2.367796162
4.091410618
3.792501246
4.368774365
4.533701117
1.535516962
2.922788554
2.917046791
2.581419692
2.73454891
3.789455105
2.237871003
2.611361363
0.755918417
0.2
3.289784941
3.73115118
3.428805476
3.074037627
2.174278927
2.032425713
2.271200265
2.264843692
2.50893168
3.517381782
3.005656252
4.736241761
2.865139412
3.665957286
3.260059235
3.911917193
3.875710957
2.401549412
1.961600588
3.450931441
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
CpG Day 1
TGFBRAP1
TGIF1
B2M
HPRT1
RPL13A
GAPDH
ACTB
HGDC
RTC
RTC
RTC
PPC
PPC
PPC
29.92649078
28.91666412
23.9241066
29.9152317
25.91753578
21.91293907
21.91828156
Undetermined
Undetermined
Undetermined
Undetermined
22.91176796
22.91159058
22.91250038
Well
A1
A2
A3
A4
A5
A6
A7
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
Sample Name
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
Target name
ACVR1
ACVR2A
ACVRL1
AMH
AMHR2
BAMBI
BGLAP
BMP1
BMP2
BMP3
BMP4
BMP5
BMP6
BMP7
BMPER
BMPR1A
BMPR1B
BMPR2
CDC25A
CDKN1A
CDKN2B
Cт
26.91287422
28.92421913
29.91112328
35.93698883
Undetermined
24.94927788
30.9241066
25.91661835
35.91916275
37.12503052
31.92014503
Undetermined
28.91017914
Undetermined
30.89986229
29.91556168
32.92647934
27.91428757
33.91455078
24.92231178
28.91001511
7.014900208
6.005073547
1.012516022
7.003641129
3.005945206
-0.9986515
-0.99330902
0.000177383
0.000909805
3.702378505
2.941493436
2.401235435
1.182007386
2.83168399
2.927651612
2.408122346
0.2
0.2
0.2
0.2
3.275207574
3.07906561
2.949639339
ΔCт Mean
Ct Threshold
4.004800797
3.219218421
6.016145706
2.020796124
7.00304985
2.532236694
13.02891541
1.957175877
0.2
2.041204453
2.323011522
8.016033173
2.786193527
3.008544922
3.922827817
13.01108932
2.480697984
14.21695709
0.142067115
9.012071609
3.301960046
0.2
6.002105713
2.474235202
0.2
7.991788864
2.55256431
7.007488251
2.432560881
10.01840591
3.078861397
5.006214142
1.532409098
11.00647736
3.097781132
2.014238358
3.790263109
6.001941681
3.032252109
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
CER1
CHRD
COL1A1
COL1A2
COL3A1
CST3
DLX2
ENG
EVI1
FKBP1B
FOS
FST
GDF2
GDF3
GDF5
GDF6
GDF7
GSC
HIPK2
ID1
ID2
IGF1
IGFBP3
IL6
INHA
INHBA
INHBB
ITGB5
ITGB7
JUN
JUNB
LEFTY1
LTBP1
LTBP2
LTBP4
MYC
NBL1
Undetermined
32.91520691
19.9069767
17.91167068
21.92803764
23.91311264
28.91373825
23.91764832
29.91020012
30.91089249
29.91200447
25.91640091
Undetermined
Undetermined
31.90870285
32.92554855
37.07022476
35.92084122
28.9154377
24.9188118
25.91355133
32.90822601
22.90353775
31.92134857
30.90125656
25.91112137
33.91283417
23.90943336
33.91505051
30.91415596
28.90751648
35.89551926
23.91410065
23.91874695
27.93000412
31.92028427
26.92002296
10.00713348
-3.00109673
-4.99640274
-0.98003578
1.005039215
6.005664825
1.00957489
7.002126694
8.002819061
7.003931046
3.008327484
9.000629425
10.01747513
14.16215134
13.01276779
6.007364273
2.010738373
3.005477905
10.00015259
-0.00453568
9.013275146
7.993183136
3.003047943
11.00476074
1.00135994
11.00697708
8.006082535
5.999443054
12.98744583
1.006027222
1.010673523
5.021930695
9.012210846
4.011949539
0.2
3.125580922
3.171573962
2.603935608
2.016481067
3.662744208
3.084787411
3.070933006
2.056827552
4.414369095
3.956409998
2.691286765
0.2
0.2
3.304875647
3.508820351
0.689790496
2.649002365
2.930632498
2.396499298
2.821161229
2.418541571
3.693028466
4.119574117
2.560452637
2.673301013
2.50826991
4.229361538
2.73471708
3.725779907
4.49076831
3.275621158
1.98281197
3.936527895
2.581441385
2.710544018
3.600788572
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
BMP Day 1
NODAL
NOG
NR0B1
PDGFB
PLAU
RUNX1
SERPINE1
SMAD1
SMAD2
SMAD3
SMAD4
SMAD5
SMURF1
SOX4
STAT1
TGFB1
TGFB1I1
TSC22D1
TGFB2
TGFB3
TGFBI
TGFBR1
TGFBR2
TGFBR3
TGFBRAP1
TGIF1
B2M
HPRT1
RPL13A
GAPDH
ACTB
HGDC
RTC
RTC
RTC
PPC
PPC
34.91054916
26.91560364
37.10988235
37.10096359
27.90843964
28.9173851
23.91061783
29.90580177
29.93912697
27.90966034
28.8975811
29.91663742
29.90494347
29.91272354
27.91121483
24.91443634
28.92496872
26.90901756
26.90779495
28.90012932
20.9067173
29.90267372
36.96798706
28.91057968
28.90716362
27.9189949
22.92194939
28.90722084
24.92043877
20.92370987
20.91905594
Undetermined
Undetermined
Undetermined
Undetermined
22.90782356
22.90807343
12.00247574
4.007530212
14.20180893
14.19289017
5.000366211
6.009311676
1.002544403
6.997728348
7.031053543
5.001586914
5.989507675
7.008563995
6.996870041
7.004650116
5.003141403
2.006362915
6.016895294
4.000944138
3.999721527
5.992055893
-2.00135612
6.994600296
14.05991364
6.002506256
5.999090195
5.010921478
0.013875961
5.999147415
2.012365341
-1.98436356
-1.98901749
-0.00024986
2.630002361
3.71860024
0.410184908
0.366177473
3.23575252
3.488916213
2.593308446
3.494397463
3.025416809
2.206281751
1.4878845
3.50474907
3.237215991
3.771431775
3.187344287
4.009134084
4.469559123
3.088933841
2.315783104
3.301403113
3.426266177
3.166723153
2.540037387
3.108279
2.793383166
2.849173302
2.444094598
1.387226232
2.24874944
2.427541291
2.295961514
0.2
0.2
0.2
0.2
3.175146078
2.971528699
H12
BMP Day 1
Well
A1
A2
A3
A4
A5
A6
A7
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
Sample Name
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
22.91523933 0.007165909
PPC
Target name
ACVR1
ACVR2A
ACVRL1
AMH
AMHR2
BAMBI
BGLAP
BMP1
BMP2
BMP3
BMP4
BMP5
BMP6
BMP7
BMPER
BMPR1A
BMPR1B
BMPR2
CDC25A
CDKN1A
CDKN2B
CER1
CHRD
COL1A1
COL1A2
COL3A1
CST3
DLX2
ENG
EVI1
FKBP1B
FOS
FST
GDF2
Cт
27.90838432
29.92850304
30.90711212
37.03230667
Undetermined
25.94823265
31.92345428
25.91089249
35.92169571
Undetermined
32.92193604
Undetermined
29.91387558
Undetermined
31.89844513
30.91596222
32.92238998
28.91483116
34.91173935
24.91330528
28.91825294
Undetermined
33.91755295
19.91369438
18.91056252
22.92554474
23.91117668
29.91604805
24.9207058
29.90859795
30.8999157
29.91336441
26.91773033
Undetermined
2.597942746
ΔCт Mean
Ct Threshold
4.998449326
3.400442718
7.018568039
2.305324444
7.997177124
3.010567793
14.12237167
0.876581953
0.2
3.038297653
2.499963719
9.013519287
4.240410009
3.000957489
3.117970186
13.01176071
1.99022573
0.2
10.01200104
3.394519078
0.2
7.003940582
2.871519779
0.2
8.988510132
3.04514394
8.006027222
2.566150507
10.01245499
2.651345293
6.004896164
1.560816968
12.00180435
2.209827909
2.003370285
3.144305825
6.008317947
2.589572848
0.2
11.00761795
4.119447163
-2.99624062
2.499402875
-3.99937248
3.336716482
0.015609741
2.17687618
1.001241684
3.109149518
7.006113052
3.089508792
2.010770798
3.948274635
6.998662949
1.629718017
7.989980698
3.095728904
7.003429413
2.937300119
4.007795334
4.007133185
0.2
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
GDF3
GDF5
GDF6
GDF7
GSC
HIPK2
ID1
ID2
IGF1
IGFBP3
IL6
INHA
INHBA
INHBB
ITGB5
ITGB7
JUN
JUNB
LEFTY1
LTBP1
LTBP2
LTBP4
MYC
NBL1
NODAL
NOG
NR0B1
PDGFB
PLAU
RUNX1
SERPINE1
SMAD1
SMAD2
SMAD3
SMAD4
SMAD5
SMURF1
Undetermined
31.90759468
32.91968918
37.01350403
35.91843796
28.91323853
25.92192841
26.91376114
34.9249115
22.90542221
30.91566086
30.90039444
25.9064827
32.90077591
23.91005898
33.91024399
30.91504097
28.91569328
37.03939056
24.90104675
24.9162674
28.93275261
32.92185211
26.92102051
34.91061783
27.91340256
Undetermined
37.1280899
27.90591431
29.91329956
23.90749168
29.90410805
29.92446327
27.9083519
29.89518738
29.9064045
29.89925385
8.997659683
10.00975418
14.10356903
13.00850296
6.003303528
3.011993408
4.003826141
12.0149765
-0.00451279
8.005725861
7.990459442
2.996547699
9.990840912
1.000123978
11.00030899
8.005105972
6.005758286
14.12945557
1.991111755
2.006332397
6.022817612
10.01191711
4.01108551
12.00068283
5.00346756
14.21815491
4.995979309
7.003364563
0.997556686
6.99417305
7.014528275
4.998416901
6.98525238
6.996469498
6.989318848
0.2
3.089797335
2.419009658
1.083769577
2.798893838
2.857738429
3.069187401
4.03788679
2.786929773
2.583875135
2.726844746
2.5580805
2.91847811
1.906298256
3.285827056
2.532063258
3.997251549
3.429298526
0.907126784
2.332372334
4.193332595
3.589922637
3.117625126
3.333717397
2.641056737
2.707622287
0.2
0.239173601
3.309060128
4.564795873
2.701798567
2.418558836
2.763110775
2.153218838
1.610847023
2.806665353
2.315162826
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
BMP/CpG Day 1
SOX4
STAT1
TGFB1
TGFB1I1
TSC22D1
TGFB2
TGFB3
TGFBI
TGFBR1
TGFBR2
TGFBR3
TGFBRAP1
TGIF1
B2M
HPRT1
RPL13A
GAPDH
ACTB
HGDC
RTC
RTC
RTC
PPC
PPC
PPC
30.9122467
28.91239357
25.91224861
28.91507721
26.90542793
27.90451813
29.90862274
21.90665817
29.90092659
36.96531677
28.91575623
28.91638184
28.91315079
23.92326927
29.92003441
24.91809464
21.91885948
20.92316246
Undetermined
Undetermined
Undetermined
Undetermined
22.90773582
22.909935
22.90537834
8.002311707
6.002458572
3.002313614
6.005142212
3.995492935
4.99458313
6.998687744
-1.00327682
6.990991592
14.05538177
6.005821228
6.006446838
6.00321579
1.013334274
7.010099411
2.008159637
-0.99107552
-1.98677254
-0.00219917
-0.00455666
3.878811198
3.300680873
4.531638849
3.85722681
2.533930814
2.583302779
4.320659418
3.61487706
2.51512414
2.440079997
2.501142453
2.361706105
3.685686707
3.080444567
1.640800958
2.124442518
3.76924695
1.71696708
0.2
0.2
0.2
0.2
3.366821126
3.008592787
3.294890468
Results TGFbeta/BMP PCR Array Day 3
Well
A1
A2
A3
A4
A5
Sample name
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Target name
ACVR1
ACVR2A
ACVRL1
AMH
AMHR2
Cт
ΔCт Mean
Ct Threshold
26.90986633 3.996873856
2.509868548
29.94871521 7.035722733
2.871555489
30.92320061 8.01020813
4.066546874
36.96560669 14.05261421
1.547861999
Undetermined
0.2
A6
A7
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
BAMBI
BGLAP
BMP1
BMP2
BMP3
BMP4
BMP5
BMP6
BMP7
BMPER
BMPR1A
BMPR1B
BMPR2
CDC25A
CDKN1A
CDKN2B
CER1
CHRD
COL1A1
COL1A2
COL3A1
CST3
DLX2
ENG
EVI1
FKBP1B
FOS
FST
GDF2
GDF3
GDF5
GDF6
GDF7
GSC
HIPK2
ID1
ID2
27.93361664
31.91731644
25.92528534
35.94216919
37.11650467
31.91350937
36.96411896
30.92293358
Undetermined
30.90221596
30.91775131
32.93107605
28.91706085
34.90523148
24.9103508
28.90938187
Undetermined
32.91299438
19.91039467
18.9177475
22.93094444
23.90937233
32.9114418
24.91796875
30.91696548
31.89914894
31.91494942
26.91492653
Undetermined
Undetermined
29.90360069
31.91735268
36.91287613
35.91047668
29.93217278
29.92893982
28.91123962
5.020624161
9.004323959
3.012292862
13.02917671
14.20351219
9.000516891
14.05112648
8.009941101
7.98922348
8.004758835
10.01808357
6.004068375
11.992239
1.997358322
5.996389389
10.00000191
-3.00259781
-3.99524498
0.017951965
0.996379852
9.998449326
2.004976273
8.003973007
8.986156464
9.00195694
4.001934052
6.990608215
9.004360199
13.99988365
12.99748421
7.019180298
7.015947342
5.998247147
2.2282856
4.138030041
3.914886575
1.444194227
0.203795654
2.996111971
1.190453427
1.942793045
0.2
3.432746893
3.101825562
3.070237918
1.850167531
3.127299264
2.683809063
4.108919255
0.2
3.620692383
2.69538639
3.665133099
2.267623267
3.361813583
2.633145863
3.685401457
2.463866753
3.54128518
4.211428528
3.417507949
0.2
0.2
2.978668723
2.497953714
3.579762265
2.928737865
3.76872228
3.44183102
4.047336286
D7
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
IGF1
IGFBP3
IL6
INHA
INHBA
INHBB
ITGB5
ITGB7
JUN
JUNB
LEFTY1
LTBP1
LTBP2
LTBP4
MYC
NBL1
NODAL
NOG
NR0B1
PDGFB
PLAU
RUNX1
SERPINE1
SMAD1
SMAD2
SMAD3
SMAD4
SMAD5
SMURF1
SOX4
STAT1
TGFB1
TGFB1I1
TSC22D1
TGFB2
TGFB3
TGFBI
35.90397263
22.90953636
28.91130447
30.9016571
26.90822601
31.90562439
23.90919685
33.91189194
29.91423416
28.90712547
34.89426041
24.8921051
23.91720772
28.9280777
31.91587639
26.92273331
34.91943741
29.90711784
36.95665359
Undetermined
26.90574455
28.91308022
22.90839577
29.9045105
29.9324913
26.90873528
29.9070015
29.91069603
29.90502167
30.91584015
27.91228676
24.91209221
28.92624855
26.91454506
27.90186882
30.91311646
22.91750526
12.99098015
-0.00345612
5.998311996
7.988664627
3.995233536
8.992631912
0.996204376
10.99889946
7.001241684
5.994132996
11.98126793
1.979112625
1.00421524
6.01508522
9.002883911
4.009740829
12.00644493
6.994125366
14.04366112
3.992752075
6.000087738
-0.00459671
6.991518021
7.019498825
3.995742798
6.994009018
6.997703552
6.99202919
8.002847672
4.999294281
1.999099731
6.013256073
4.001552582
4.988876343
8.000123978
0.004512787
2.232487322
2.724409144
3.141234539
3.636070199
4.285943745
1.471474743
3.233796262
2.963496506
2.884066946
4.05389044
3.078863299
2.444519065
2.805520456
3.274321572
2.818659848
2.780217412
1.952042675
3.398221364
1.531231881
0.2
2.795734842
3.798592211
2.855644284
3.117511927
2.984673523
2.035073227
2.304245209
3.25848592
2.123525059
3.902734298
3.01620201
2.76164019
3.841732455
3.549204259
2.57434824
4.124895488
3.272943268
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Untreated Day 3
Well
A1
A2
A3
A4
A5
A6
A7
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
Sample Name
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
TGFBR1
TGFBR2
TGFBR3
TGFBRAP1
TGIF1
B2M
HPRT1
RPL13A
GAPDH
ACTB
HGDC
RTC
RTC
RTC
PPC
PPC
PPC
29.89934731
36.94369888
29.91915321
28.91436195
27.91550064
22.92736244
28.91673279
24.91773224
20.91569519
21.91934395
Undetermined
Undetermined
Undetermined
Undetermined
22.91023445
22.91299248
22.90982819
Cт
ACVR1
ACVR2A
ACVRL1
AMH
AMHR2
BAMBI
BGLAP
BMP1
BMP2
BMP3
BMP4
BMP5
BMP6
BMP7
BMPER
BMPR1A
BMPR1B
BMPR2
26.91404152
28.93214226
30.92336655
36.93084335
37.09082031
26.92736244
30.91866875
25.92175484
35.94266129
36.94519043
30.90514755
Undetermined
30.91741753
Undetermined
30.90338898
30.91576004
31.92596436
28.93115044
6.986354828
14.03070641
7.006160736
6.001369476
5.002508163
0.014369965
6.003740311
2.004739761
-1.99729729
-0.99364853
-0.00275803
-0.00316429
2.968316232
3.083438266
4.115903139
2.886295767
2.93088812
2.221152749
1.565296787
2.969211949
2.492496223
2.257700751
0.2
0.2
0.2
0.2
3.229916144
2.943548936
3.11298144
ΔCт Mean
Ct Threshold
3.997215271
3.547527613
6.01531601
1.924076719
8.006540298
4.270410705
14.01401711
2.178050684
14.17399406
0.268611041
4.010536194
1.981876154
8.001842499
2.72095221
3.004928589
3.897904495
13.02583504
1.635983831
14.02836418
1.433426834
7.988321304
2.137546789
0.2
8.000591278
2.907283796
0.2
7.986562729
3.207502668
7.998933792
3.471450357
9.009138107
2.760577768
6.014324188
2.085784922
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CDC25A
CDKN1A
CDKN2B
CER1
CHRD
COL1A1
COL1A2
COL3A1
CST3
DLX2
ENG
EVI1
FKBP1B
FOS
FST
GDF2
GDF3
GDF5
GDF6
GDF7
GSC
HIPK2
ID1
ID2
IGF1
IGFBP3
IL6
INHA
INHBA
INHBB
ITGB5
ITGB7
JUN
JUNB
LEFTY1
LTBP1
LTBP2
35.91432953
24.92440414
27.90775681
Undetermined
31.91435623
19.91452026
17.91428375
22.94019699
22.91328049
31.90937424
24.92167854
29.90849495
31.90513039
31.91906357
25.91457367
Undetermined
37.17736435
30.90525627
31.91552734
36.91786957
34.92662048
28.91825485
28.92877769
26.91742897
35.89774323
22.91725731
28.92467499
30.89758301
25.90513611
30.90638924
23.91036606
33.91422272
29.91321373
28.90798759
34.91379166
24.89955521
23.91396141
12.99750328
2.007577896
4.990930557
8.997529984
-3.00230598
-5.0025425
0.023370743
-0.00354576
8.992547989
2.004852295
6.991668701
8.988304138
9.00223732
2.997747421
14.2605381
7.988430023
8.998701096
14.00104332
12.00979424
6.001428604
6.011951447
4.000602722
12.98091698
0.000431061
6.00784874
7.98075676
2.98830986
7.989562988
0.99353981
10.99739647
6.996387482
5.991161346
11.99696541
1.982728958
0.997135162
2.586646991
3.884171399
3.36354384
0.2
3.77946451
3.118927929
2.379753092
1.822680657
3.143959069
2.592947253
3.843491658
2.127001793
3.922018558
4.462023883
3.107166779
0.2
0.022170407
3.13599746
2.386965848
3.02395297
2.4019294
3.075399606
3.508938742
2.684551202
2.328624648
2.484466043
2.44437954
3.477960655
4.070986028
1.453344799
4.345438999
2.837025155
3.422246664
4.688744639
2.777695379
2.547349892
3.043153955
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
LTBP4
MYC
NBL1
NODAL
NOG
NR0B1
PDGFB
PLAU
RUNX1
SERPINE1
SMAD1
SMAD2
SMAD3
SMAD4
SMAD5
SMURF1
SOX4
STAT1
TGFB1
TGFB1I1
TSC22D1
TGFB2
TGFB3
TGFBI
TGFBR1
TGFBR2
TGFBR3
TGFBRAP1
TGIF1
B2M
HPRT1
RPL13A
GAPDH
ACTB
HGDC
RTC
RTC
28.92639351
31.91313934
25.92126846
33.9124527
29.91213608
36.97555923
37.08218765
26.9083271
28.92072105
23.91345406
29.90341568
28.92926788
26.91095352
29.91724014
28.92474365
29.89781189
29.91829491
27.91176987
24.90878105
28.92617226
25.91196442
27.90651512
29.91507912
22.9184761
28.9109993
36.93802643
28.91064644
27.91685867
27.9152832
22.93213844
28.93483925
24.92432213
20.91586304
21.92827988
Undetermined
37.03987885
Undetermined
6.009567261
8.996313095
3.004442215
10.99562645
6.99530983
14.05873299
14.1653614
3.991500854
6.003894806
0.996627808
6.986589432
6.012441635
3.994127274
7.000413895
6.007917404
6.980985641
7.001468658
4.994943619
1.991954803
6.009346008
2.995138168
4.989688873
6.998252869
0.001649857
5.99417305
14.02120018
5.99382019
5.000032425
4.998456955
0.015312195
6.018013
2.00749588
-2.00096321
-0.98854637
14.1230526
3.413483603
2.299217243
2.857302688
2.852842916
3.330878821
1.486678985
0.397080369
3.154778293
4.897206374
3.974459387
3.137703491
2.598952053
2.137797152
2.473247357
2.209699388
2.557149982
3.083385503
3.064298705
3.228245438
4.00398961
2.206704028
2.459342024
2.464459943
2.584126614
2.170934445
3.347723068
3.187145386
2.254979291
3.385910139
3.571586515
1.375115094
3.230023762
2.556155537
2.639039918
0.2
0.009088887
0.2
H9
H10
H11
H12
CpG Day 3
CpG Day 3
CpG Day 3
CpG Day 3
Well
A1
A2
A3
A4
A5
A6
A7
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
Sample Name
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
RTC
PPC
PPC
PPC
Undetermined
22.91355515
-0.0032711
22.91682625
22.92004013 0.003213882
Cт
ACVR1
ACVR2A
ACVRL1
AMH
AMHR2
BAMBI
BGLAP
BMP1
BMP2
BMP3
BMP4
BMP5
BMP6
BMP7
BMPER
BMPR1A
BMPR1B
BMPR2
CDC25A
CDKN1A
CDKN2B
CER1
CHRD
COL1A1
COL1A2
COL3A1
CST3
DLX2
ENG
EVI1
FKBP1B
26.91155624
30.95024872
30.91044044
36.92390442
Undetermined
24.92677116
31.91807556
26.91378784
35.92972946
Undetermined
31.90452957
Undetermined
29.90961647
Undetermined
32.89747238
30.92432594
34.92628479
28.91864586
34.91463089
24.91252518
29.90604591
Undetermined
32.91565704
20.91287422
18.90805817
23.93588257
23.90780258
29.92125702
23.91682243
30.90521049
31.91469765
0.2
2.92878513
2.817259447
2.540304898
ΔCт Mean
Ct Threshold
4.005199432
2.426012933
8.043891907
3.06879311
8.004083633
3.839950657
14.01754761
2.368661307
0.2
2.020414352
1.615257089
9.01171875
4.114322055
4.00743103
3.776268984
13.02337265
1.843337207
0.2
8.99817276
2.45885149
0.2
7.003259659
2.626359902
0.2
9.99111557
3.339383724
8.017969131
2.294386967
12.01992798
2.883011135
6.012289047
1.881208159
12.00827408
3.407373558
2.006168365
2.604369102
6.999689102
3.303498827
0.2
10.00930023
2.918185951
-1.99348259
2.944689535
-3.99829865
2.843100124
1.029525757
3.061291188
1.00144577
2.578672134
7.014900208
3.182971385
1.010465622
2.899569631
7.998853683
1.815607063
9.008340836
4.553365329
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
FOS
FST
GDF2
GDF3
GDF5
GDF6
GDF7
GSC
HIPK2
ID1
ID2
IGF1
IGFBP3
IL6
INHA
INHBA
INHBB
ITGB5
ITGB7
JUN
JUNB
LEFTY1
LTBP1
LTBP2
LTBP4
MYC
NBL1
NODAL
NOG
NR0B1
PDGFB
PLAU
RUNX1
SERPINE1
SMAD1
SMAD2
SMAD3
30.91270065
26.91368866
Undetermined
Undetermined
32.90131378
32.91111755
36.98396683
35.91360092
29.92276192
25.91524887
26.92047882
32.90332031
24.90755463
32.91189575
30.9040184
27.90794182
32.90487289
24.91052246
33.91016388
31.92362976
28.90230942
35.90419769
25.9000721
24.91542625
28.92594528
31.92501831
26.91616249
34.91312408
26.91028023
36.98078918
37.02585602
28.91169357
28.91631126
24.90895653
29.90335274
29.92209435
27.90901375
8.006343842
4.007331848
9.99495697
10.00476074
14.07761002
13.00724411
7.016405106
3.008892059
4.014122009
9.996963501
2.001197815
10.00553894
7.997661591
5.001585007
9.998516083
2.004165649
11.00380707
9.017272949
5.995952606
12.99784088
2.993715286
2.009069443
6.01958847
9.018661499
4.009805679
12.00676727
4.003923416
14.07443237
14.11949921
6.005336761
6.009954453
2.002599716
6.996995926
7.015737534
5.002656937
4.35402827
3.66508462
0.2
0.2
3.165090019
2.611072085
1.438200247
2.874302571
3.555740275
2.491601971
4.471650604
1.953351287
3.888197911
2.711121383
2.403107496
4.442151578
1.473608351
4.434910014
3.013209938
4.418461568
3.945098306
3.418231565
2.487646497
4.15172214
3.541434117
1.983782025
3.839129443
2.287603123
3.23415194
1.336275595
0.680197828
3.43863371
3.212802897
2.652837996
3.285583282
2.66367194
2.103459362
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
BMP Day 3
Well
A1
A2
A3
A4
A5
A6
A7
Sample Name
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
SMAD4
SMAD5
SMURF1
SOX4
STAT1
TGFB1
TGFB1I1
TSC22D1
TGFB2
TGFB3
TGFBI
TGFBR1
TGFBR2
TGFBR3
TGFBRAP1
TGIF1
B2M
HPRT1
RPL13A
GAPDH
ACTB
HGDC
RTC
RTC
RTC
PPC
PPC
PPC
29.89793587
29.91085625
29.90171814
29.91597176
28.91981888
24.90943527
28.93177414
27.91584015
28.89968109
29.89870262
22.90909195
30.89844322
36.99396133
29.91234589
29.92074966
28.91399384
23.91529274
29.93642235
24.91737175
21.91476059
21.91616631
Undetermined
Undetermined
Undetermined
Undetermined
22.90719604
22.90635681
22.91175652
Cт
ACVR1
ACVR2A
ACVRL1
AMH
AMHR2
BAMBI
BGLAP
26.91195297
29.93468094
29.91283989
36.93755341
37.08799362
25.94844246
31.91670418
6.991579056
7.004499435
6.995361328
7.009614944
6.013462067
2.003078461
6.025417328
5.009483337
5.99332428
6.99234581
0.002735138
7.992086411
14.08760452
7.005989075
7.014392853
6.007637024
1.008935928
7.030065536
2.011014938
-0.99159622
-0.99019051
0.000839233
0.005399704
1.821515607
3.039685026
2.207243383
4.050943818
2.349020302
3.316329786
2.759341156
3.515467234
3.041741318
3.396798141
3.802335724
2.932130239
1.329838083
2.595904397
3.659170191
3.086615893
2.663358841
1.885050125
2.699931787
3.484799372
2.081967392
0.2
0.2
0.2
0.2
3.775376822
3.418773568
2.775465227
ΔCт Mean
Ct Threshold
4.004951477
2.854930329
7.027679443
1.981476329
7.005838394
2.796039094
14.03055191
2.069619918
14.18099213
0.335270125
3.041440964
2.592019771
9.009702682
4.139738221
A8
A9
A10
A11
A12
B1
B2
B3
B4
B5
B6
B7
B8
B9
B10
B11
B12
C1
C2
C3
C4
C5
C6
C7
C8
C9
C10
C11
C12
D1
D2
D3
D4
D5
D6
D7
D8
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP1
BMP2
BMP3
BMP4
BMP5
BMP6
BMP7
BMPER
BMPR1A
BMPR1B
BMPR2
CDC25A
CDKN1A
CDKN2B
CER1
CHRD
COL1A1
COL1A2
COL3A1
CST3
DLX2
ENG
EVI1
FKBP1B
FOS
FST
GDF2
GDF3
GDF5
GDF6
GDF7
GSC
HIPK2
ID1
ID2
IGF1
IGFBP3
25.91504478
35.92279053
Undetermined
31.91039848
36.99066925
29.9324913
Undetermined
32.90099335
30.91948891
32.91895676
28.90703583
36.95022964
24.91514587
28.90414047
Undetermined
32.91453552
20.90647125
18.90664291
23.93585587
22.912323
29.91246033
23.91874123
29.91395187
30.90834427
30.91374397
26.92363739
Undetermined
Undetermined
32.90665436
33.91713333
37.1139679
36.90000916
29.9330616
25.91274071
25.9101181
33.90484238
24.90123749
3.008043289
13.01578903
9.003396988
14.08366776
7.025489807
9.993991852
8.012487411
10.01195526
6.000034332
14.04322815
2.008144379
5.997138977
10.00753403
-2.00053024
-4.00035858
1.02885437
0.005321503
7.005458832
1.011739731
7.006950378
8.001342773
8.006742477
4.016635895
9.999652863
11.01013184
14.2069664
13.99300766
7.026060104
3.005739212
3.003116608
10.99784088
1.994235992
2.421276452
2.200778093
0.2
2.938063725
1.004399729
1.783288185
0.2
2.991367239
2.969893739
2.21089585
1.832004274
1.488917719
3.308145675
3.152518109
0.2
3.228549635
3.078638574
3.095282812
2.919551002
2.603572518
2.371380575
2.752471564
1.683874204
2.591750598
4.326575511
4.155010034
0.2
0.2
3.398243738
2.791638915
0.428452616
3.872359826
4.011655179
2.780484337
3.148532993
1.81572133
3.609216241
D9
D10
D11
D12
E1
E2
E3
E4
E5
E6
E7
E8
E9
E10
E11
E12
F1
F2
F3
F4
F5
F6
F7
F8
F9
F10
F11
F12
G1
G2
G3
G4
G5
G6
G7
G8
G9
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
IL6
INHA
INHBA
INHBB
ITGB5
ITGB7
JUN
JUNB
LEFTY1
LTBP1
LTBP2
LTBP4
MYC
NBL1
NODAL
NOG
NR0B1
PDGFB
PLAU
RUNX1
SERPINE1
SMAD1
SMAD2
SMAD3
SMAD4
SMAD5
SMURF1
SOX4
STAT1
TGFB1
TGFB1I1
TSC22D1
TGFB2
TGFB3
TGFBI
TGFBR1
TGFBR2
31.91114235
30.9010334
25.9122963
32.89814377
23.91117859
33.91424561
30.91346741
28.90116692
34.91271591
25.90320396
24.91751099
28.91685486
32.92017746
25.9214325
34.92274857
27.91852951
37.06748199
37.09172058
27.90900993
28.90455818
24.90822411
29.9090004
28.91108131
27.90423203
29.90258217
29.91688347
29.90395737
29.92173386
28.91230392
24.91017151
28.9167366
26.91849899
28.89783478
29.90007973
22.9161911
29.90473747
36.93506622
9.004140854
7.994031906
3.0052948
9.991142273
1.004177094
11.00724411
8.006465912
5.994165421
12.00571442
2.996202469
2.010509491
6.009853363
10.01317596
3.014431
12.01574707
5.011528015
14.1604805
14.18471909
5.002008438
5.997556686
2.00122261
7.001998901
6.004079819
4.99723053
6.995580673
7.009881973
6.996955872
7.014732361
6.005302429
2.003170013
6.009735107
4.011497498
5.990833282
6.993078232
0.009189606
6.997735977
14.02806473
2.680480368
2.498630657
2.598501473
1.990673642
3.298392616
2.722675424
3.655759962
4.859991368
2.49482627
2.977915403
3.919887849
3.063259765
3.031982559
2.802456942
3.660172443
4.100242588
0.598930572
0.01070269
2.523466885
3.907068548
2.910327288
2.70452505
1.968003976
2.411208145
1.764775328
3.391852739
2.254755155
2.998411279
2.373834306
3.42234747
3.04669587
2.207375015
3.334837638
3.310445617
2.739520219
2.758139554
2.871550577
G10
G11
G12
H1
H2
H3
H4
H5
H6
H7
H8
H9
H10
H11
H12
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
BMP/CpG Day3
TGFBR3
TGFBRAP1
TGIF1
B2M
HPRT1
RPL13A
GAPDH
ACTB
HGDC
RTC
RTC
RTC
PPC
PPC
PPC
29.91222
28.90660477
28.91010094
22.92100716
28.90535545
24.91982079
21.92122269
21.92228127
Undetermined
37.12774658
37.16362381
Undetermined
22.90724182
22.9070015
22.90865517
7.005218506
5.999603271
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