From www.bloodjournal.org by guest on June 18, 2017. For personal use only.
An
Unidentified
Reticuloendothelial
Marrow
Report
By
T
ARTHUR
HE
study
parable
cell
Mflschlin’
pigment
a marrow
in which
in
HYIAN
AND
workers
in separate
of
the
here
literature
with
assOciatiols
routine
hematologic
remained
in good
were
youmig
au
HYMAN
institutions
of an
of tw’o patiemsts,
disclosed
in bone
cells
were
B.
.JULIAX
homse marrow
previously
idemitical
reported
Of symptoms
its the
A review
mentioned
Bone
Studies
marrow
a similar
cell in splenic
pumscture
as
Later,’7
how’ever,
he stated
that
film
patients
A.
cell
cases.
been
Histochemical
itsdepetsdemst.
blue
in
Spleen
with
GEORGE
two
of these
had
and
Cases
of large
described
makrophage.”
Both
The
have
by
type
detailed
Two
SAWITSKY,
FINDING
unusual
seen
of
Cell
led to a
that
mso (‘om-
studies
although
“em so geisamsust blauhe had more
recemstly
observed.
adults
who
appreciable
showed
a relative
enlargement
findings
in both
patietits
health
during
a follow-up
paucity
of liver
amid
spleeus.
were tiormal.
Both
patients
period
of three
arsd a half
years.
CASE
Case
1
S.
D.,
a 34
year
Presbyteriams
This
three
had
day
casionally
Physical
examination
findings
examination
agai n revealed
summarized
ulcerations
ansd
schistosoma
nsaussoni
repeat
Six
test.
The
stools
shortly.
were
and
July
which
required
spontaneous
Platelet count
From
the
Jamaica,
N.
Submitted
The
Blunt,
no
was
authors
M.D.
for
ss
essentially
two
and
parasites.
The
found,
the
Departments
and
November
of
the
for three
23,
are indebted
anud A. Kritzler,
1953;
to Miss
M.D.
to
dimtrrhea.
liver
Physical
laborat
ory
no mucosal
schistosomes.
occasion
A
but
vomiting
a ten
direct
negative
test
and
and
Presbyterian
The
.
year
subsided
without
received
day episodes
in
oti
a
negative.
was
disappeared
s!a’
was
for
publication
of
1947
related
week
to 300,000),
Presbyterian
of diarrhea
December
a two
Pathology
of the
accepted
the
admitted
described
disclosed
oc-
one
diarrhea
Mazzini
nausea
patient
vomiting
anud
Service
Juan
of
a two
were
course
of
asymptomatic.
of
Medicinue
Medical
the
over
San
thse
watery
symptonis
left
for
anud
continues!
The
ons one
of
of
enlargement
negative
1:1000
no treatmetit.
Her only
new
complaint
ecchymoses
on the arms
and legs for
was
150,000
percu.
nsm.
(normal
200,000
Y.,
had
l)reviouslY
examination
was
at
except
episodes
12,
These
vomitinig
positive
discharged
well
mild
ever
of
5 cm.
and
anspulla
were
age
Clinuic
complained
daily.
subsequently
of nsausea
negative
ova
Vaisderbilt
patient
patient
firm,
immediately
was
amehae
arsd
and
rectal
was
test
atsayoidits
1948,
the
test
negative
Although
emetine
and
The patient
in
Mantoux
the
stools
he hepatosplenonsegaly
table
1. Sigmoidoscopic
t
skini
since
The
was
days
The
mso lymphadenopathy.
patient
three
of
biopsy
was
ins the
1946.
four
a smooth,
There
the
after
to
vomiting.
disclosed
but
1946,
a year
three
and
of the spleen.
therapy
are
of
usausea
wn-ss seen
February
or twice
consisted
wit-h
in August
housewife,
in
once
and
symptomatic
Hospital
Rican
York,
occurred
period
cm. enlargement
on
Puerto
New
associated
before.
3
old
Hospital,
diarrhea.
or
REPORTS
the
to
period
red
March
the
in
atid
Queens
Hospital,
in March
atid
977
1949,
appearance
of
November
white
General
New
1948.
cell
counsts
Hospital,
York,
N.
V.
1, 1954.
LillianiLesser for the microphotographs
and
their
help with the histochemical
studies.
for
1947
August
to
J. W.
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978
RETICULOENDOTHELIAL
CELL
TAB LE
(Grn.)/cunnn.
Year
1946
(%)
14.2
MARROW
SPLEEN
Ceph.floc.
: S.
4
AND
Findings
Lymphs
74
7400
BONE
I -Laboratory
Patient
1947
1948
IN
D.
Remarks
(Case
22
1)
4 plus
3 plus
12.7
67
6900
2
31
Platelets-150,000;
2 plus
-2’lO”;
1949
l3.68800
1950
14.5
13.8
1953
8000
8400
67
2
76
57
1
1
I
31
3 plus
A/G
23
2 plus
Prothrombin
=
42
3 plus
Platelets-160,000;
Patietst:
J. A. (Case
Gm.
%
time
=
13 sec.
Prothromsi-
16 sec.
=
2)
Normal
1943-1946
1949
13.5
80
8600
20
01
1 plus
Platelets-175,000;
time-2’;
.
Bleeding
time-lO’;
Coag.
Prothrombini-15”
5.1/3.0
were
normal
She
table
(see
spontaneous
1),
bruising
was
only
timsse
time-5’15”
5.0/3.8
time
bits
Bleed.
Coag.
laboratory
were
the
bleeding
and
clot-timig
%
The
tendency
times.
A/G
=
toward
continued.
readmitted
additional
as
;
Gm.
to
finding
findings
are
Presbyterian
Hospital
on physical
examination
summarized
in
table
ons May
4,
was that
1. A stumdy
for
1950,
of chronic
of
the
re-evaluation.
cystic
films
The
mastitis.
obtained
by
The
repeated
bone marrow aspirations
revealed
two abnormal
cell types,
similar
to those
of case 2 (fig. 1)
described
below. The other formed elements
of the marrow
were
normal
as listed
in table
2.
In November
1953,
a liver aspiration
biopsy revealed
nornial liver parenchyma.
None of
the above cell types could be found after careful study
A marrow
aspirate
was found
to be
unchanged
from
that
reported
previously
The
patient
has
now
been
followed
for four
years, is working
full time and has no complaints
other than a tendency
to bruise
easily.
.
.
Case 2
J.
A.,
May
was
well
process
short
for
until
1941,
right,
months
and
increase
in
The
no
the
mouth
findings
distress
and
was
normal,
bilateral
auscultation.
hut the
The
liver
spleen
The
was
was
was
six
enlarged
palpable
there
heart
were
was
8 cm.
Army
chest
was
fever,
and
was
film
gastric
of a well
were
or
loss
infiltrative
following
were
a
for
disclosed
nine
a further
Otisville.
of
in
patienst
During
weight
always
all
were
ex-
negative
for
advice.
developed,
bilateral
an
Sanitorium
to
against
those
showed
film
washings
There
hospital
The
infiltration
Otisville
anorexia,
discharged
Genseral
significant.
a repeat
ill.
below
not
readmitted
were
palpable
Queens
further
at
later,
well
pterygia
nourished,
and
white
pingueculea,
small
tiontender
posterior
cervical
lymph
noted.
The lungs were clear to percussion
was
entirely
below
revealed
patient
adenopathy
to 5 cm.
was
months
sputa
appearing
axillary
to
history
hospitalized
the
patient
oms admission
not
x-ray
chills,
of
the
admitted
preinduction
and
studies
1946,
past
repeat
hemoptysis,
In
was
The
process
coughing,
Minimal
male,
a routine
infiltrative
physical
nodes.
when
organisms.
in
Italian
tuberculosis.
Bacteriologic
fast
male
and
then
the
no
perienced.
white
upper lobe. A
bed rest. The patient
discharged.
About
of
time,
acid
old
pulmonary
in the
period
this
year
a 26
1949,
normal.
the
the
costal
costa!
The
margin
margin
abdomen
and
and
and
nontender
was
was
smooth
soft
and
nontender.
was
smooth
and
niontender.
From www.bloodjournal.org by guest on June 18, 2017. For personal use only.
A.
I,
I
G .
\vi t hu blue-st
‘l’li(
ext
a ,‘ea
of 3 tue right
The
Manut
were
ictssit
ics
tiat
alkahitse
tcst
was
muegative
ions
of
Lmphs
4).
the
laboratory
nosle
biopsy
Sarcoid-hike
IL
:iSl)i
uat
lobe
.
.
(‘best
flat
for
blood
(‘(I giaiuules
x-ray
acid-fast
t
he
rv
were
also
data
is sunismarizes!
Repeated
in
was
‘1’. ISLE
auldl
nuoted.
Sternum
May
urcs
was
1)100(1
(\t
oplasrui
13(551 al)i(’al
ospleniouusegmt
anusl gust
ive.
titus,
nc
rates
were
linut
ic lesion
(xaminuat
wit
ion,
ansi
nmornsal.
lu (euut
ral
(hue
hone
of
‘Flue
sascat
Sternum
May
‘49
J. A.
Ilium
June
49
3.()
0.5
1.0
17.0
15.0
uuon-segnsented
25.()
34.0
23.0
w
segmetited
20.()
ISo
19.5
23.0
tnvelocvtes
Losinophils
3.()
2.0
Basophils
1 .0
11 .0
L\nuph0c\tes
Reticulum
Plasma
Ervthroid!
cells
cells
eleusse,uts
Cellularity
?slegakaryocyt
1 .5
es
9.0
16.3
Sternum
June
‘so
20.3
1.3
.3
0.3
13.0
12.0
15.7
2.5
2.3
1 .0
2 .0
1 .7
21 .0
19.0)
16.0
20.7
2.0
I .0)
1St)
0.7
23.3
.7
Norussal
Nornsal
Presenut
Present
ion
nuiarrow
.1 spi r(Itlon
2.0
19.()
Neutro.
(It’-
iIIP
(Percent)
Blasts
lv.
rugs
washui
phud)sl)hiorus,
Patient
‘50
‘Fh,e
i 11 1 hl(’
uuegat
calci
at ionu
S. D.
June
.
hepat
ouuly
cult
test
The
hyperplast
.‘lIu,rou’
Sternum
55)
uni
st ai n
:111(1 shi:upes
1.
Re1)eated
2.-Bone
Patient
-
979
i V(’ lesiouu
shuo\ved!
sedinietit
table
a productive
disclosed
arranugenuuenut
I uufilt rat
Mazziuui
ruon’tssal
llYM.N
nig .si zc
c(l sI)ut
The
were
tiormal.
all
13.
) -%Vnighut
va ,\i
abdonienu
repeat
bacilli.
sluetnist
of
revcale(l
of
I)late
1: 1(XX) but
at
J.
AND
I ( oi I -insuuse,’sisnu
ionu , (aSP
.‘-\.
positive
tlYM.X
A.
ai iui tug pigluienul
luottusal
phosphatase
retisainuinig
(fig.
\cle
upper
oux
always
cmii
flialI’O\\.
-ld)IiP
.---
is packe(l
S.WI’I’SKY,
1 .7
1 .7
17.0
From www.bloodjournal.org by guest on June 18, 2017. For personal use only.
lt1TI(UL(i)ENDOTtIELI.L
980
Fl
(
idenut
.
2
. -.
ifs-
anitI
Boric
.
l:u mge
uuiarmov
h3oiue
marrow,
en(Iot
s were
helial
in
-like
ret iculoenuslot
Splenuic
lie
oplasumsic
2 (oil
ptmnst
(fig.
tire,
c(!
case
,
cells
)
it
AND
MARRO\V
on )-\Vu’i
Ii (‘cectut
wit
SPLEEN
gist
t’is’ally
st ai n
1)lstcedl
‘flue
.
si nugle
a mmows
nucleus
hu blue
pigniemit
I uopsy
sells
:ist
ccl
ni muua mt’o
gmauiules
and
hot Ii of whichi are
oplasnii,
hueli :d
I a huvpempl
0 huese eletsient we me many
st :u Iii ng
(vt
st :ui
2) shuowe
among
t lie Si lvemunaui
net isuloetudot
-Wriglut
st ai nu (fig.
i oui ant!
wit hi a foansv
usiuug
BONE
2 (oi 1-insnsemsi
-Ii ke
inuniemsionu
1 propomt
tel Is ptucked
cell
ioti
IX
gu’auuules.
hi ‘uS’might
wit
muomnsa
hielial
1)lue-)igunenut
tat
belial
(‘usc
fn’ouiu si en’nuumuu anud i 1iunu
eleussent
aspi
met isuloensiot
st ai uui rug syt
1)lue
13.
-.
t V()
(‘ELL
t echoic,
nee!le
I:uito
sinai
revealed
in
Tlue
fommuie
I
ret iculo-
type
amsother
described
seetu
those
.
Ia rge
of
below.
ams abuns!ance
thue marrow
of
smear’s
3).
The
cli ruical
charged
conudi
to
t
t
he
course
out
hiltsreniai
ion
iii
-pat
tuesi
t lie
ienut
goosh
huost)it
s!epa
,
:il
arud
ti0
i’nut i mclv
was
mt nsient
.
lien
For
the
svnspt
Ml.:’l’itODs
unusvemit
past
OniS
OF
t
bree
om’ fins!i
fu!
aiud
ears,
usgs
t lie
t lue
have
patient
patient
was
‘s
(!is-
general
appeared.
STUDY
Morp/wlojj
‘j’\vo
abtsormisal
cell
tVI)eS
\v(ue
lu()te(l
itt
the 1)Omme
fl5IStTOW’
staitued with
Wright’s
stain
A lam’ge
ret iculoemidotlselial-like
cell
wit
Is
a
simsgle muimcleims usually
es’cemi-
From www.bloodjournal.org by guest on June 18, 2017. For personal use only.
A.
FIG
.
‘uVuighut
3.-Spleuiic
st ailu
trically
punct
Diffuse
.
ret i(’uloenudot
placed.
pla.smic
9acked
whose
(figs.
1,
needle
nuomnial
t
arehuit
o 0 huosc
seer,
(‘Isrotnatmns
mote
HYMAN
biopsy
(‘it
i
B.
nu t lie
I onue
re.sensl)le(l
obscured
b’
umucleoli
vere
fruemstly
that
the
-
ed
azure
blue
scat
temed
tPt
(‘ytoplasmis
sizes
to
ttlm(l shapes
(leep
a
i(’ttlo(‘vto-
Flse
of variable
light
of
sul)et’inspose(l
oI)serve(l.
grausules
a very
l)htue-)ignu(nut
tis:i r’ron
arrausgenseust
pigmeumte(I
)
(oi 1 -insnsersiouu
en’s of
dust
was
from
9S1
tecbtuic
by
tire
detail
blue-staimsing
varied
J.
AND
2-Silvertssauu
msuclear
or
HYM.-\.N
(‘a
blu(’
3).
2,
the
Otie
imstemssitv
A large,
2.
like
fine
.1.
of
nuclear
hut-
w’ith
stain
case
ccl ls si nsi lam
‘flie
gramsimles.
was
ure,
-li ke
(‘ells
G.
rel)lacensenut
huehial
essdothelial
SA\VITSKY,
foamisy
first
mcticuloemt(lothelial
(‘(‘11 described,
ttmscoinmomsly
noted
its
this
the
cell
appeared
Ione
vitls
to
be
(lark
t nt-
gn’aunmlcs.
a macrophage
of
the
type
umot
inarrow.
Ilistochernisiry
Films
of house
studies.
1.
The
Snsears
pinkish
marm’ov
staimsed
cells.
with
the
2.
The
nuclei
Smears
tious
3.
a pale
plasmic
would
blue.
to
iusdicate
that
blue5
granules
of the
a \‘ery
staiused
iso change
The
and
di(l
failimre
suggested
asl)irate
ivere
cytoplasnsic
pale
piusk
react
gramsules
color.
its the
for
used
were oht.aimse 1:
t lie periodic
aci(l
Sclsiff
of mucopolysaccharides
exposed
Toluidiume
muss
stainitsg
preseusce
times revealed
react
itt ilizimig
Isomogeneous
fled
pam’ti(’les
fol lowimsg
This
tlse
ion
of the
i
Iiistochensical
‘
result ed
unmidenut
was
couusisteuut
reactioum
granules.
staimsing
with
staitsimsg
capacity
of
the
were usot glycogemi.
The nuclear
clsromatini
meactioni
to occur
by this technic
its
ahseisce
of msucleoprotei
us nsaterial.
(‘ytoplasmic
miot staius
of
ausy
the
the
periodic
the
gramsules.
acid
SchifT techThis
oI)serva-
before
its the
diastase
itt
large
iuiclusioums
stained
cytoplasns.
the
cvto-
a
i-
From www.bloodjournal.org by guest on June 18, 2017. For personal use only.
9S2
ttu:rl(’uuAoI:XD()’I’ulm:LI.-\.L
F’u ; . 4
hon’den’
VlL5
lint
LvIuiI)hu
.
of cells
4. Bouuc
ive
r)
.
1. se of
uuilikely
tIsat
1hsis
(u
tiOt((l
5(
of
of
(lesoxvmil)ose
I’
mnauiv
of
spleeum
of
mmoted
tIme
‘suspe(’t
acid
t hiese
vas
of
hut
tells
shoavi
rug a
\Vn’ighut
si aitu
115(1 huodfit
msssu Ic it
\vlui(’hu
I)y
suggeste(l
the
Feulgems
the
results
tecisiii(.ui
uio stainsinig
placed
shsoved
did
tcchmmics
of
thse
Imu this
(‘01 oplasiss
or
thuat
imsto
(listille(l
water
the
granules
were
umot disclose
thse
pmeseuuce
of
air-
then
usot
water
iron
iii
any
present
rep
)rtel
younmg
)scussmox
the
its
heniatologic
two
I
lie
1)1(5(9
3(11111
of
tisese
cases
findinugs,
ice
male
of
sinii
wills
cells
iii
su(’hs
asynsptoniatit
as
ammd beisigim
tlsev
Ftmrt hemmore,
svuudrome.
mesemishle
course
I
lam 1)1 ste pigmeu
itiiexplaiuied
a niaruow
lie
pos-
lIe has
a patient
from
dei mi I lie
nsacro)hsages
spleniomegaly.
filmis ohtaiuied
a
Moschslin’
case
ml ed
hepato-
imsdicate
sluice
with
(‘huon mit’ nsalauia.’’
In aus attempt
were
.
pueseust.
aspiuate
teclsnics
staiuuimug
nornsal
occurrence
ed
node
is present
ase.
t o st aiuu
vete
staiuse(l,
maumow
Wright
sinsilauities
a
fai led
mo(lium(’atious
‘as
l)oume
viths
iroum
sihle
semi
nu . lAvniiphi
ion
(11tP5ti011.
spleisonsegalv,
melated
h)(M 1. II e
uu :111(1 eosi
u’:tl case:tt
‘(lit
l)lsoSI)hsat
miil(’lei(
a
I
‘rise
(
ulote(I.
was
smeats
(1
I ,
acids
fatty
material
gu’aimules
)luhle.
8. The usual
the cells its
.
SPLEEN
obtainable.
riot
I I I
or
staiume(l
amud stained
splcetu
ioum of’ alkalimme
fats
, ttIl(’lettu
(‘ytoplasmic
7. Thick
‘t’e
)ol-su(!ami
mietitual
smears
(‘Xl)(uit’fl(umt
dried
i(-canh
It(’(t
ions
‘isualizat
for
abseisce
viths
Se(’t
in
.Nt)
stiicats l)u’(l)an((lI’ a mno(!ificat i()ti ()f I he ( omisoui
ti5Utt0\\
negat
\‘(tP
1(1)11(0
hot ed
MAIIItOW
BONE
IN
2-- -huenmsat oxvli
s’tse
,
l:uu’ t o t hose
arid
Iiia(Ie
I (lousY
nuo(Ie
siuni
(‘I:IAuA
miumdet’taken
to
(letemmimme
m. Lu ut eupuet
thse
nature
at itum i of
hist
of
these
ocheniical
granules,
fiuidiuugs
histochiemical
has
mssany
studies
pit falls.
mm
From www.bloodjournal.org by guest on June 18, 2017. For personal use only.
A.
SAWITSKY,
G.
A.
TABLE3.-Comparisofl
of
tIYMAN
Tissue
AND
.‘tfast
Mast
J.
B.
983
HYMAN
Cellsu’ith
A uthors’
Cells
cells
Authors’
cells
C’toplasm
A.
Wright’s
stain
Intense
Cytoplasmic
Shape
membrane
of granules
Size
of
1)lue
(!efinsite
Uniiformly
gransules
Stainsed
violet
Not
round
toluidinme
blue
Not
slefiniite
Small
1)eep
to
blue
greenu
Varied
I’rsiforns
with
Sea
blue
to
large
Unstained
blue
Nucleus
B.
l4Ocat
ion
Houtmsl
Nucleoli
C.
oval
or
cent
ceust ral
or
ec
Sanse
-
nc
Xonue
(1arrow
cellularity
However,
Usually
both
iii
t 0 contain
Frequent
cases
studied
hsypoplastic
al)ove,
Nortssally
the
grausimles
seem
active
idemstical
amsd appear
mucopolysaccharides.
Its case
2, both
infiltratioms
by
distribution
of
opinion
that
the
the
boise
cell
this
cell
these
marrow
type
cells
ammd the
its question.
throimghout.
differ
the
from
splemsic
This
aspirat
jots
the
possibility
raised
revealed
reticimloendothelial
amsy previously
marked
of
system
described
the
‘ide
It.
is
our
reticuloemmdothelial
cell, macrophage,
or mast
(CII. After
reviewimsg
our slides,
Moschlius’2
stated
that
he believed
them
to he similar
to those
he had pmeviously
sects its splemsic aspirates.
Because
certaims
similarities
to the mast
(,elPi, 14 might
be postumlated,
table
3
is included
for comparisoms.
The tahle
shovs
that
the laboratory
flmsdimsgs differ
between
the
two types
of cells. There
is also a marked
differeusce
its the chiusical
the patiemits
comscermsed.
The
patiemsts
Fademt5
studied
usually
had
prognoses,
whereas
our cases
were pract ically
sympt om-free
mdi ‘idual.s.
pictures
of
grave
Both
of our
clinically
From
the
origin
such
patiemsts
have
msov
morphologic
followed
vealed
in
the
granules.
mamsy
Simice
l)oIie
presemsce
The
st.aimsing
appearamsce
indicated.
seems
cells
for
of
latter
three
amid
al)msom’mal
(‘eli,
somiasis
while
the
granulomatous
The
a half
and
‘eaus
are
secousd
and
amid
are
had
first
tissue
biopsy.
1)0th
sparse
gramsularity
butt
ausd
umsually
iemst was
of
a
are
similam
pat ieust s were
(‘hni(’al
of havimsg
schisto-
“gramiumlomatoums”
uso (‘ommous
umusderlying
suspecte(1
reactious
disease
of
re-
similar
umo (‘ommous
suspected
for
preseumt
‘s st au m. Tlsese
Wright
patiemsts
searched
examiumatious
morphologically
evideusce
Thus,
our
pat
have
this
with
disorder
our
we
Occasiomsally,
vi t h
hematologic
that
a reticulocusdothelial
cases,
dells
showum
examimied
two
has
havimsg
a
beens
(‘hroumic
process.
fat.her
aspiratiolss
aspirates
have
whets
its a cervical
lymph
usode
revealed
to date,
although
these
ueticuloemsdothelial
cells
properties
of the
observimsg
marrow
fouuid to have mso primary
syndrome.
It is of imiterest
We
I)eeis
svell
uisable
sister
of the
complete
to
postulate
patiemmt
physical
in case
examiusatiomms
thse
fumsction
2 w(9
of
examiused.
these
Or sigusificausce
subjects
of these
Bomse
marrow
were
miormal.
cells
at
this
From www.bloodjournal.org by guest on June 18, 2017. For personal use only.
984
IIETICULOENDOTHELIAL
time
We hope
described
above
and
its possible
(‘ELL
IN
BONE
that
other
investigators
may
amsd help clarify
the frequency
relationship
to a symsdrome
MARROW
.ND
SPLEEN
compare
their
flisdings
with
those
and meaniusg
of the cell described
iiscludimsg
obscure
hepatospleno-
megaly.
SUMMARY
I
A cell
.
is described
its the
bone
not previously
been
fully
described.
filled
with
blue-staimsilsg
grausules.
marrow
films
2. These
and
were
from
were
(‘ompared
The
3.
aspirated
cells
case
also
to
sternum
reported.
5 The
sigmsificatsce
from
striking
iliac
of two
patients
which
has
a case
amsd fuusctiomi
from
omse of the
summarized
by
similarities
of the
graisular
of these
two
This
material
unusual
cells
patients
M#{246}schlin.
its the
individuals.
symptom-free
stusdies
cell
bone
crest.
aspiratioms
clinical
relatively
a commos
syndrome
is preselst.
4. I)ifferential
histochemical
are
and
its a spletsic
cells
show
in
aspirates
This
is a large
reticuloendothelial-hike
This
cell has been
foutsd
in successive
foumsd
similar
reports
hepatosplenomegaly
marrow
patietsts,
in the
its the
i.e.
suggests
that
cytoplasm
marrow
is un-
known.
SUMMARIO
( 1 ) Es describite
in
detahio.
Illo
pat.ientes
Ihlo
que
frottis
(2)
grande
cellula
previemente
osseo-medullar
de apparentia
non
de
esseva
duo
reportate
differente
reticuloendothelial
e plenate
prende
colorantes
blau.
Iste typo
de cellula
esseva
trovate
in
de medulla
ossee aspirate
ab le sterno
e le cresta
iliac.
Specimens
del mesme
typo
de cellula
esseva
et.iam
trovat.e
in un aspirasplenie
ab un del patientes
Illos esseva
comparate
con simile
cellulas
trovate
un
(3)
in
es un
INTERLINGUA
typo
de cellula
que
trovate
in aspiratos
esseva
de granulos
successive
tion
un
IN
caso
summarisate
Le
Mflschhin
per
reportos
de
nostre
duo
casos
Ins ambes
hepatosplenomegahia
es presente
es relat.ivemente
libere
de sympt.omas.
drome
(4)
commun.
Es reportate
cytoplasma
(5) Le
stumdios
signification
e
exhibi
Isto
histochimic
Ic fuunct ion
frappante
durante
suggere
differential
de
iste
similarit.at.es
cellulas
que
ii
del
materia
se
inusumal
in
clinic.
Ic
qume alteremente
individusos
de
tracta
urn syn-
granular
Ic medulla
in
non
Ic
es
cognoscite.
1EFERlNCI’S
S.:
I)ie
1
MOESCnILIN,
2
Ergebnisse,
Gnmcx, D.:
l3asel,
Techniques
LILLIE,
I).:
R.
WmsLocmu,
G.
acid-Schiff
4:
-,
562,
Histopathuologic
0.,
Techsuiik,
ion:
Bennso
Schwabe,
of Histoand
iii
J. J.,
various
Khinmischis!agnostischse
1947.
Cytochemistry,
Technique,
RHEmNG0LD,
reaction
AND
miormal
New
Philadelphia,
E.
DEMPSEY,
cells
of
the
umn(l hetssatologisc.hse
\ork,
Insterscieuice,
Blakistoni,
W.:
The
blood
occurrence
arud
1949.
1948.
coninmective
of the
tissue.
periodic
Blood
1949.
BUNTING,
relationsship
\Iilzpuuskt
11.,
to
AND
unucopoly
DESIPSEY,
saccharides.
E.
\V.:
Metachnomasia
Atu.
J.
Anat.
in
81:
tisaninnaliaui
1, 1947.
tissues
and
its
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6
G.: Distribution
GOMORI,
-:
Physiol.
17:
Distribution
71,
phosphatase
poort
D.:
Fat
Bact.
56:
262,
1944.
Blood
J. J.
i2
MoEscHL!N,
‘
MICHELS,
3: 641,
York,
reference
FADEM,
S.
New York,
MOESCHLIN,
19:
by
organs
under
985
HYMAN
and tissues.
normal
A histochensical
169,
J. Cell.
& Comp.
and
under
pathological
study
of “acid”
and
G
“alkaline”
5: 580,
1949.
Onderste-
1944.
Sudaus
WIsLocKI,
dyes
B.:
suspended
ins
Histochemical
watery
methods
media.
applied
J.
to
Path.
&
hematology.
AND
J.:
cells,
1938,
MONTAGNA,
Bone
1951.
communication,
mast
Hoeber,
to alkaline
R. S.: Tissue
16 LEITNER,
‘
The
B.
C. H.
14 NOBACK,
AND
tissues
in
D.:
Ind.
staining
Personal
A.:
Paul
B.
1948.
S.:
N.
ims normal
J.
in normal human
bone marrow
smears.
Blood
method
of staining
lipids,
acetic-carbol-Sudans.
Animal
A.
RHEINGOLD,
‘
Sci.
GOVAN,
#{176}
AND
1941.
ANDREUCCI,
AND
An improved
J. Vet.
HYMAN
of phosphatase
distribution
C.:
A.
1941.
M.
Rnmxovn’rcui,
JACKSON,
G.
of acid phosphatase
Arch. Paths. 32: 189,
conditions.
10
SAWITSKY,
in Downey,
vol.
1.
W:
Some
Hal
hlansdbook
(e!.):
histochemical
aspects
Marrow
Biopsy.
Haematology
ins the
Ilensatology,
of mast
phosphatase
and cytochrome
oxidase.
Anat.
mast cells in human bone marrow.
Blood 6:
Grune & Stratton,
S.: Spleen
Puncture,
of
Light
cells
Rec.
614,
93:
New
with
special
279,
1946.
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of Sternal
Puncture,
1949.
New York,
Grune
&
Stratton,
1951,
pp.
18,
29.
From www.bloodjournal.org by guest on June 18, 2017. For personal use only.
1954 9: 977-985
An Unidentified Reticuloendothelial Cell in Bone Marrow and Spleen:
Report of Two Cases with Histochemical Studies
ARTHUR SAWITSKY, GEORGE A. HYMAN and JULIAN B. HYMAN
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