HTS Grade ATP ␥-33P
+
[␥33P]-ATP
Kinase
and
33
[␥- P]-ATP
Step 1. The kinase substrate is captured on the FlashPlate
surface by streptavidin or another capture agent.
Step 2. The kinase and
isotopically-labeled ATP
([␥-33P]-ATP) are added
to the FlashPlate well.
Benefits
Step 3. The labeled 33P is incorporated into the captured
substrate during the kinase reaction. This brings the
labeled 33P into close proximity to the scintillant coating
on the FlashPlate. The resulting signal is detected on a
microplate scintillation counter.
Features
• EasyTides ATP ␥- P HTS Grade
• EasyTides ATP, ␥-33P HTS Grade
• Optimized for use in Scintillation Proximity Kinase Assays
• 3000 Ci (111 TBq)/mmol
• High Specific Activity
• 10 mCi/mL (370 MBq/mL)
• Ready-to-Use; Shipped Ambient for Storage at 2-8 ºC
or Below
• Packaged in 10 mM Tricine Buffer, Stabilizer
and Amber Dye
®
33
• Shipped Ambient, Store at 2-8 ºC or Below
• Fresh Every Friday
Product number
• NEG602K250UC 250 µCi (9.25 MBq)
• NEG602K001MC 1 mCi (37 MBq)
Kinase Assay Solutions
Radiochemicals
Description
Product Name
Quantity
Product No.
High specific activity ATP, [γ-33P]-,
especially for kinase assays
Adenosine 5'-triphosphate, [γ-33P]-, (EasyTides),
3000 Ci (111 TBq)/mmol
250 µCi (9.25 MBq)
1 mCi (37 MBq)
NEG602K
FlashPlate and Image FlashPlate Microplates
Description
Product Name
Quantity
Product No.
FlashPlate® Plus Products
Myelin Basic Protein FlashPlate PLUS, 96-well
5 plates
SMP106001PK
FlashPlate microplates
Nickel Chelate FlashPlate PLUS, 96-well
5 plates
SMP107001PK
pre-coated with secondary
antibodies or other proteins.
Nickel Chelate FlashPlate HTS PLUS, 384-well
2 plates
10 plates
SMP412001PK
SMP412A001PK
Phospholipid FlashPlate PLUS, 96-well
5 plates
20 plates
SMP108001PK
SMP108A001PK
Phospholipid FlashPlate HTS PLUS, 384-well
2 plates
10 plates
100 plates
SMP414001PK
SMP414A001PK
SMP414J001PK
Phospholipid FlashPlate coating buffer, 10X concentrate*
1 x 50 mL
1 x 200 mL
SMP900001EA
SMP900A001EA
Streptavidin FlashPlate PLUS, High Capacity, 96-well
5 plates
20 plates
100 plates
200 plates
SMP103001PK
SMP103A001PK
SMP103J001PK
SMP103B001PK
Streptavidin FlashPlate HTS PLUS, High Capacity, 384-well
2 plates
10 plates
100 plates
SMP410001PK
SMP410A001PK
SMP410J001PK
Streptavidin-coated Image FlashPlate-384 Plus
5 plates
40 plates
RMP151E001PK
RMP151001PK
Phospholipid-coated Image FlashPlate
5 plates
40 plates
100 plates
RMP153E001PK
RMP153001PK
RMP153J001PK
Image FlashPlate Products
Pre-coated Image FlashPlate microplates.
* Buffer without IBMX
Please inquire about our custom coatings and bar coding services.
Complementary Instrumentation
PerkinElmer's high throughput and ultra-high throughput readers and imagers plus our advanced assay technologies equal maximum screening productivity
and cost-effectiveness for all your kinase assays.
®
• LumiLux Cellular Screening Platform
• VICTOR™3 Multilabel Plate Readers
®
• ViewLux ultraHTS Microplate Imager
®
®
• TopCount and MicroBeta Microplate Scintillation and Luminescence
®
• EnVision Multilabel Plate Readers
PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
Phone: (800) 762-4000 or
(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/lasoffices
©2007 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks of PerkinElmer, Inc. VICTOR is a trademark and EasyTides, EnVision, LumiLux,
MicroBeta, ViewLux and TopCount are registered trademarks of PerkinElmer, Inc. or its subsidiaries, in the United States and other countries.All other trademarks not owned by
PerkinElmer, Inc. or its subsidiaries that are depicted herein are the property of their respective owners. PerkinElmer reserves the right to change this document at any time without notice
and disclaims liability for editorial, pictorial or typographical errors.
007850_01
Printed in USA