AGRICULTURE AND BIOLOGY JOURNAL OF NORTH AMERICA ISSN Print: 2151-7517, ISSN Online: 2151-7525, doi:10.5251/abjna.2014.5.1.33.39 © 2014, ScienceHuβ, http://www.scihub.org/ABJNA Quality evaluation of composite millet-wheat Chinchin Adegunwa, M. O1*., Ganiyu, A. A2., Bakare, H.A1 and Adebowale, A.A2 1 Department of Hospitality and Tourism, Federal University of Agriculture, Abeokuta, Nigeria. Department of Food Science and Technology, Federal University of Agriculture, Abeokuta, Nigeria. * Corresponding author: ([email protected]; +2348033581392) 2 ABSTRACT This study was conducted to evaluate the quality of chinchin made from millet-wheat composite blend. Millet grain was processed into flour and used to substitute wheat flour at different portion of (0, 10, 30, 50, 70, 90 and 100). The functional and pasting properties of the different millet-wheat flour blends were determined. The composite flour was thereafter, processed into chinchin and the proximate composition and sensory evaluation of the chinchin were determined. There were significant differences (P<0.05) in the functional properties of composite flour in term of bulk density, dispersibility and water absorption index (WAI) ranging from 0.76-0.83g/ml,73.50-78.00 % and 0.77-1.22g/g respectively. Similarly significant differences (P>0.05) were also observed in term of swelling index, swelling capacity and water absorption capacities (WAC) of functional properties with values ranging from 1.47-1.79V/v, 0.47-0.79V/v and 5-10g/g respectively. There were significant differences (P<0.05) in the pasting profile of the wheat-millet flour blends except for pasting temperature where there was no significant differences (P>0.05). There were significant differences (P<0.05) in the proximate composition of the chinchin samples with values ranging from 3.98-5.04%, 12.63-19.99% ,7.56-8.13%, 4.94-6.10%, 4.56-5.23% and 57.41-63.35% for moisture, protein, fat, ash, crude fibre and carbohydrate contents respectively. There were also significant difference (P<0.05) in sensory attributes of the chinchin samples parameter measured. The study concluded that chinchin of acceptable quality comparable to the 100% wheat flour chinchin are obtainable from millet-wheat composite flour up to 30% millet substitution. Keywords: Millet, Wheat, Chinchin, proximate, pasting properties INTRODUCTION The rapid urbanization and increase in population in recent years have resulted in an increase in the consumption of wheat based product especially chinchin in sub-Saharan Africa especially in Nigeria. This has spurred pragmatic research on compositing flour from locally cultivated cereals to reduce wheat importation. Millets, like sorghum, are predominantly starchy. The protein content in millet is very close to that of wheat, both provide about 11% protein by weight, on a dry matter basis (USDA, 2012). Millets are also rich in B vitamins (especially niacin, B6 and folic acid), calcium, iron, potassium, magnesium, and zinc (USDA, 2012). Millets contain no gluten, as none of the millets are closely related to wheat; they are therefore appropriate foods for those with celiac disease or other forms of allergies /intolerance of wheat. Therefore processing into flour or use as composite will help accruing this benefits for human use. Deep fried foods have a satisfying crunchy texture and a distinctive taste which are widely enjoyed. Many dishes cooked by this method are quite inexpensive and so popular in low budget outlet. Also most snacks are products from deep frying process. In Nigeria and throughout the world, snacks foods are widely spread and eaten among kids and adults because of their convenience both in preparation and circulation and also because of their distinctive taste. Matz (1993) defined snack foods as being something consumed primarily for pleasure rather than for social or nutritive purpose and not ordinarily used in a regular meal. Chinchin is a traditional Nigerian snack prepared using wheat flour, butter, milk and eggs from which a stiff paste is made which is then deep fried until golden brown and crisp. It is quite popular across Nigeria and most part of western Africa. It is sweet to taste, slightly hard and may be equated to a harder version of a doughnut occasionally, the chinchin might even contain cowpeas and at times it may be prepared by baking instead of frying (Akubor, 2004). Agric. Biol. J. N. Am., 2014, 5(1): 33-39 o Groundnut Meg might be employed as a flavouring agent along with the other usual customary baking materials used in its preparation. fryer reached 180 C, the dough cubes were placed in the hot oil and the chinchin was deep fried for 8minutes until golden brown. The fried chinchin was removed and drain off excess oil before serving (Akubor, 2004) The normal practice involves the dough making process with the ingredient combined and kneaded together to form firm dough. In the next step the dough is uniformly flattered out and small square shaped pieces of about ¼ inch thickness are cut out (Mepba et al, 2007). These are then deep fried to form the famous chinchin. As such chinchin is easily one of the most favoured food item, a much relished African pastry which could serve as a dessert, snack and also as a popular street food. Infact it enjoys a very special place in the heart as well as stomachs of West African population (Mepba et al, 2007). The chinchin is one food item, that invites a great deal of flexibility in terms of the ingredient used and method of preparation involved, while some like to eat it hard and crunchy, other prefer a softer easier to chew version. The purpose of this research is to evaluate the quality of chinchin produced from composite Wheat-Millet flour. Analytical Determination Proximate composition: protein, fat, minerals, crude fibre, moisture and carbohydrates contents were determined using AOAC 2004 method. Functional properties Water Absorption Capacity and Water Absorption Index was determined using the method of Wang and Kinsella (1976). Bulk density was determined using the method of Wang and Kinsella (1976). Swelling capacity and Swelling Index was determined using the method of Ukpabi and Ndimele (1990). Dispersibility was determined using Kulkarni et al (1991). Pasting property was determined by using rapid visco-analyzer (IITA, 2001). Sensory evaluation: The sensory attributes of the chinchin was obtained by using simple hedonic tests as described by Larmond (1991). This was done using a 20 member panel comprising of students of the department, who were familiar with the sensory attributes of chinchin. Each panelist was asked to score each attribute on a 9 point hedonic scale where 1 and 9 represent dislike extremely and like extremely respectively. The attributes that were evaluated includes colour, taste, flavour, texture, appearance, crispness and overall acceptability. MATERIALS AND METHODS The materials used were purchased from Osiele market in Abeokuta, Ogun state, Nigeria and they includes Millet grains (pearl millet), Wheat flour, Sugar, Margarine, Baking powder, Eggs, Milk and Vegetable oil. Production of Millet and Composite flour: The millet grain was free of dirt, dust, stones and other extraneous materials in order to ensure wholesomeness. The grain was then grounded into powder using hammer mill, the flour was passed through a 250 µm mesh sieve to obtain fine millet flour (Oloyo, 1999). Statistical analysis: All data collected were subjected to analysis of variance (anova) using SPSS (version 15, 2007). Duncan multiple range test was used to separate the differences in the mean scores. RESULTS AND DISCUSSION Composite flour was prepared by substituting millet flour for wheat flour (i.e. millet- wheat flour) in the percentage proportion of 0:100, 10:90,30:70, 50:50, 70:30,90:10 and 100:0% respectively. Functional properties of Millet-Wheat composite flour: Table 1 present the functional properties of millet-wheat composite flour. The functional properties are those parameters that determine the application and end use of food materials for various food products. The bulk density of the flour sample ranged between 0.76-0.83g/ml. There were significant difference (P<0.05) in the bulk density. Sample (100M0W) had the least value with 0.76g/ml, while samples (90M10W), (70M30W) and (50M50W) had the same value of 0.83g/ml. The bulk density is generally affected by particle size and the density of flour or flour blend and it is very important in Preparation of chinchin: The composite flour, sugar, butter, egg, baking powder, water, and milk were mixed together at appropriate rate in a large bowl. The dough was placed on a floured surface and kneaded until smooth and elastic. The kneaded dough was rolled out to approximately 2cm thickness and then cut into small squares 2cm by 2cm in size. The oil was put inside a deep fryer MC 1800 model and allowed to be hot enough until the temperature of 34 Agric. Biol. J. N. Am., 2014, 5(1): 33-39 determining the packaging requirement, raw materials handling and application in wet processing in food industry (Adebowale et al; 2008; 2012; Ajanaku et al;2012). The dispersibility of 100% wheat and millet flours was lower than the millet-wheat composite flour samples. However, the values of dispersibility are relatively high for all the composite flour samples. Hence, they will easily reconstitute to give fine constituent dough during mixing (Adebowale et al; 2008; 2012). The water absorption capacity range from 5-10g/g, there is a significant differences (P>0.05). Samples (100M0W), (90M10W) and (70M30W) had the highest values, these values reduces gradually with decrease in substituting millet then remain stable. This could be indicative of the fact that addition of millet at higher rate confers high water binding capacity to wheat flour, which in turns improves the reconstitution ability (Ajanaku et al., 2012; Adebowale et al., 2012). High water absorption capacity is attributed to loose structure of starch polymers while low values indicate the compactness of the structure (Adebowale et al., 2005; 2012.). The water absorption index range from 0.77-1.22g/g there is a significant differences (P<0.05). 100% millet had the highest value while sample (10M90W) had the lowest value; it was observed that there is an increase in value of water absorption index as the amount of millet substitution increase in wheat flour. The swelling index and swelling capacity range from 1.47-1.79 and 0.47-0.79 respectively, there is a significant differences (P>0.05), both had lower values for 100% millet and relatively high value for composite flour. Moorthy and Ramanujan (1986) reported that the swelling power of flour granule is an indication of the extent of associative forces within the granules. Swelling capacity can also be related to the water absorption index of the starch-based flour during heating. Table 1: Functional Properties of Millet-Wheat Composite Flour Sample Bulk density Dispersibility (%) Swelling index Swelling capacity (V/v) (V/v) (g/ml) bc 73.50 d 1.60 0.83 a 78.00 a 1.75 30M70W 0.79 b 75.00 c 1.79 50M50W 0.83 a 76.00 b 1.74 70M30W 0.83 a 75.00 c 1.71 90M10W 0.83 a 75.00 c 1.57 100M0W 0.76 c 73.50 d 1.47 0M100W 0.77 10M90W bcd 0.60 ab 0.75 a ab WAC WAI (g/g) (g/g) bcd 7.50 ab 0.92 d ab 5.00 b 0.77 e 0.79 a 5.00 b 0.92 d 0.81 a 5.00 b 1.09 abc 10.00 a 1.14 b cd 10.00 a 1.15 b d 10.00 a 1.22 a abc 0.71 cd 0.57 d 0.47 c WAC-Water Absorption Capacity, WAI-Water Absorption Index Values are means of two replicates. Means in the same column with different superscript are significantly different (P<0.05). M (millet) and W(wheat) Pasting properties of Millet- Wheat composite flour: Table 2 shows the pasting properties of the different composite millet-wheat flour. The pasting property is one of the most important properties that influence quality and aesthetic consideration in the food industry since they affect texture and digestibility as well as the end use of starch based food commodities (Onweluzo and Nnamuchi, 2009 ). property as proportion of millet increases. The value ranged from 68-197RVU. There was significant differences (P<0.05) in peak viscosity of the composite flour. High peak viscosity indicates high starch content and this could explain why 100% wheat flour sample had highest peak viscosity while100% millet had the lowest peak viscosity. Significant decrease was also observed in the final viscosity as the amount of millet substitution increases. The final viscosity is a measure of stability of the granules, the value ranged from 115-280RVU. 100% millet flour had the least value while 100% Peak viscosity is an index of the ability of starchbased food to swell freely before their physical breakdown (Sanni et al., 2006; Adebowale et al., 2008). There was decrease in the values of this 35 Agric. Biol. J. N. Am., 2014, 5(1): 33-39 wheat flour had the highest value, it was observed that the value of final viscosity decreases as the rate of substitution of millet flour increases. The low viscosities of 68RVU (peak viscosity) and115RVU (final viscosity) observed in 100% millet is nutritionally beneficial in infant formulas (Fasasi et al., 2005; Osundahunsi and Aworh, 2002).100% wheat flour with highest final viscosity value of 280RVU will be more stable under certain conditions than other composite sample (Fasasi, 2009). Trough is the minimum viscosity value in the constant temperature phase of the RVA pasting profile and it measures the ability of the paste to withstand breakdown during cooling. This property also decreases with increase in millet substitution. The value ranged between 46133RVU with sample (50W50M) composite flour having the lowest value and 100% wheat flour having the highest value. There was significant increase (P<0.05) in breakdown value as rate of substitution of wheat flour in millet flour increases, 100% wheat flour (64RVU) had the highest value of breakdown while 100% millet (17RVU) had the lowest value of breakdown. The setback viscosity ranged from 64159RVU. There was significant differences (P<0.05) in setback viscosity of composite flour samples. Sample (10M90W) had the highest value of setback. The higher the setback, the lower the retrogradation of the flour paste during cooling and the lower the stalling rate of the product made from the flour (Adeyemi and Idowu, 1990). Peak time which is a measure of the cooking time ranged between 5.536.16 minutes with 100% wheat flour having the highest value of 6.16 minutes. There were significant difference (P<0.05) in the peak time among samples. o The pasting temperature ranged between 78.07 C o and 84.37 C. It was observed that there was no significant differences (P>0.05) in pasting temperature among all the samples. The pasting temperature provides an indication of minimum temperature required for cooking the samples, the pasting temperature obtained for the composite flours were quite close. A higher pasting temperature indicates higher water binding capacity, higher gelatinization tendency and lower swelling properties of starch based flour due to high degree of association between starch granules (Adebowale et al; 2008; 2012). Proximate composition of Millet-Wheat composite Chinchin: Table 3 shows the proximate composition of millet-wheat composite chinchin. The moisture content ranged from 3.98-5.04% with 30M70W chinchin having the highest value while sample (10M90W) chinchin had the lowest value. Generally there was reduction in moisture contents as the rate of millet substitution is increase. The values were within the range reported to have no adverse effect on quality attribute of the product (Mepha et al, 2007). Sanni et al (2006) reported that the lower the moisture content of a product to be stored the better the shelf stability of such products. Hence, low moisture ensures higher shelf stability of dried product. The moisture content of a food is indicative of the dry matter in that food however, low residual moisture content in confectionaries is advantageous in that microbial proliferation is reduced and storage life may be prolonged if stored inside appropriate packaging materials under good environment condition. There were significant differences (P<0.05) in protein content of composite chinchin, 100 % millet chinchin had the highest value of protein content of 19.99% while chinchin sample (90M10W) had the lowest value of 12.63%. It was observed that there was decrease in protein content as level of millet substitution was increase. The high value protein content noticed in 100% millet may be as a result of the various processes in which whole wheat grain undergone before being finally processed to all purpose white flour which might in turn had reducing effect on protein content of 100% wheat flour, as millet for this research work was not subjected to such treatment. 36 Agric. Biol. J. N. Am., 2014, 5(1): 33-39 Table 2: pasting properties of millet- wheat composite flour Sample Peak viscosity RVU Trough RVU Breakdown RVU Final Setback RVU Viscosity RVU Peak Time Min Pasting o temperature C 0M100W 197.00 a 133.00 a 64.00 a 280.00 a 146.00 b 6.16 a 78.70 a 10M90W 161.00 b 100.20 b 61.50 a 259.00 b 159.00 a 6.00 b 84.37 a 30M70W 104.50 c 57.00 c 46.30 b 202.00 c 145.50 b 5.63 c 79.85 a 50M50W 82.50 d 36.30 c 166.80 d 121.00 c 5.53 c 78.07 a 70M30W d 5.53 c 78.62 a e 5.63 c 79.55 a f 5.53 c 79.50 a d 46.00 75.00 de 48.00 cd 27.00 d 148.50 e 101.00 90M10W 74.00 de 55.00 cd 20.00 e 139.00 e 84.00 100M0W 68.00 e 51.50 cd 17.00 e 115.00 f 64.00 M (millet) and W(wheat) Values are means of two replicates. Means in the same column with different superscript are significantly different (P<0.05). The fat content ranged from 7.56-8.13%, sample (10M90W) recorded the lowest fat content of 7.56% while sample (30M70W) had the highest fat content. Fasasi (2009) reported that low fat content in a dry product will help in increasing the shelf life of the sample by decreasing the chances of rancidity and also contribute to low energy value of the food product while high fat content product will have high energy value and promotes lipid oxidation. The ash content ranged from 4.94-6.10%, increase in ash content was observed as the proportion of millet substitution was increase except for sample 50M50W which had overall least value. The ash content indicate a rough estimation of the mineral content of product, sample (90M10W) had the largest ash content of 6.10% while sample (50M50W) had the lowest ash content. There were significant differences (P<0.05) in crude fibre and carbohydrate content of chinchin samples of composite flour , the crude fibre content ranged from 4.56-5.23%, sample (30M70W) had the lowest value of crude fibre of 4.56% while sample (90M10W) had the highest value with 5.23%. Similarly the carbohydrate content ranged from 57.41-63.35%, sample (0M100W) chinchin had the lowest value of 57.41% of carbohydrate while sample (90M10W) had the highest carbohydrate content of 63.35%. The high value of crude fibre and carbohydrate observed in chinchin of sample (90M10W) may be as a result of the sample having the largest millet substitution in wheat flour at 90% millet substitution. Table 3: proximate composition of millet-wheat composite chinchin Sample Protein% 0M100W 19.50 10M90W 18.56 30M70W 16.38 50M50W 15.63 70M30W Moisture% b 4.90 c 3.98 e Fat% Ash% b 8.02 b 4.97 f 7.56 e 5.34 5.04 a 8.13 a 5.58 f 4.57 e 7.81 d 17.62 d 4.71 d 8.02 90M10W 12.63 g 4.72 d 7.96 100M0W 19.99 a 4.81 c 8.03 Crude fibre% f 5.19 d 4.84 c 4.56 4.94 g 5.13 b 5.76 b c 6.10 b 5.29 Carbohydrate% b 57.41 f f 59.70 g 60.30 c 61.91 b 4.90 e 58.97 e a 5.23 a 63.35 a e 5.11 d 56.76 g d c M (millet) and W(wheat). Values are means of two replicates. Means in the same column with different superscript are significantly different (P<0.05). 37 Agric. Biol. J. N. Am., 2014, 5(1): 33-39 Sensory evaluation of millet-wheat composite chinchin: Table 4 shows the result of the sensory evaluation of millet-wheat chinchin samples. There were significant differences (P<0.05) in all the attributes measured. The values of appearance and colour attributes ranged from 4.50-8.30 and 4.60-8.40 respectively. Generally decreases in the values of these attributes were observed as millet substitution increases, in both cases sample (0M100W) had the highest value of 8.30 and 8.40 respectively while sample (100M0W) had the lowest values of 4.50 and 4.60 respectively. The high value observed from 100% wheat chinchin may be as a result of changes in appearance and colour of chinchin as a result of addition of millet which had impact on both appearance and colour as a result of greenish colour of millet flour compared with whitish colour of all purpose wheat flour. It was also observed that likeness in terms of colour and appearance decreases as millet substitution increases and thus accounted for low value of likeness obtained from 100% millet chinchin. There were significant differences (P<0.05) in both flavour and taste attributes. Sample (0M100W) had the highest likeness in terms of taste and flavour with values of 7.40 and 7.55 respectively, while sample (100M0W) had the lowest values of 5.45 and 5.75 in term of taste and flavour respectively. Similarly it was also observed that there were no significant differences in taste and flavour among samples. The texture value ranged from 5.55-7.80, 100 %Wheat had the highest value of likeness of 7.80 while 100% millet sample had the lowest value of likeness in terms of texture. There was significant differences (P<0.05) in crispness of chinchin samples. 00% wheat chinchin had the highest value of crispness of 7.30 while100 % millet chinchin had the lowest value of 5.00. It was observed that there were no significant differences in likeness in term of crispness among samples. Significant differences (P<0.05) were observed among samples of chinchin in terms of overall acceptability.100% wheat chinchin had the highest value of likeness with 8.10,while 100% millet had the lowest value of 5.60. It was observed that likeness of chinchin sample decreases with increase in millet substitution. Similarly, there were no significant difference in overall acceptability among samples (0M100W), (10M90W) and (30M70W). This shows that the panellist still preferred millet substitution of up to 30% in term of overall acceptability. Table 4: sensory evaluation of millet-wheat composite chinchin Sample Appearance Colour Texture Flavour Crispness Taste Overall acceptability 0M100W 8.30 a 8.40 a 7.80 a 7.55 a 7.30 a 7.40 a 8.10 10M90W 7.70 a 7.70 a 7.00 30M70W 6.55 b 6.40 b 6.75 50M50W 6.20 b 5.95 b 6.15 70M30W 6.05 b 5.85 b 90M10W 4.90 c 4.90 100M0W 4.50 c 4.60 a ab 7.45 a 7.10 a 7.35 a 7.35 ab bc 6.70 ab 6.55 ab 6.60 ab 7.30 ab bcd 6.55 ab 6.45 ab 6.45 ab 6.65 5.80 cd 6.30 b 5.75 bc 6.35 ab 6.50 c 5.85 cd 5.90 b 5.35 c 5.85 b 5.80 c 5.55 d 5.75 b 5.00 c 5.45 b 5.60 bc c cd d M(millet) and W(wheat).Values are means of two replicates. Means in the same column with different superscript are significantly different (P<0.05). CONCLUSION developing countries where the crops has not been optimally utilized. 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