i
i
0 LD50
-
THE ABILITY OF IMMUNOLOGICAL RESISTANCE OF
WARMBLOODED ANIMALS WITH PRESENCE OF ORGANIC
SUBSTANCES ON THE BASE OF GLYCOLS
E.V. Sirenko
The Medical Postgraduate Academy of Kharkov
SUMMARY
The ability of TG «Rosa» to damage immunological resistance in doses of 1/100 and 1/1000 LD50 was
found in the experiment on the warm-blooded animals. It was revealed the occurring of hole-cell, the
occurring of functional activity splenar – cells, timus and the increase of plasma – cells reaction in the splenar
and lymphatic nodules of animals. The obtained data show the decrease and exhaustion of organism regulatory mechanisms.
KEY WORDS: plasma-cells, immunological resistance, organic substances on the base of glycol’s
Candida
Candida
Staphylococcus s
Candida –
-
in vitro
19
-
4
639
Helicobacter pylori»
U
in vivo
].
-
-
Pseudomonas batumici
Salmonella typhimurium
[16].
-
-
Helicobacter pylori.
–
–
-
–
–
–
-95% [9, 10, 11].
-
-
–
in vitro
H ylori
Staphylococcus aureus ATCC
25923 (F-49), Escherichia coli ATCC 25922 (FCandida
(C.albicans
C.tropicalis
S.aureus
–
S.epidermidis - 4
E.coli C.albicans –
-
20
Generator GENbox microaer (bioMerieux
1:2
2
CL –
2
85% N2.
SCL –
–
–
+ –
10;
–
0
-
-
-
Bauer-Kirbi
-
Microsoft Excel
Biostat-4"
McFarland
Densi-La-Meter (Lachema
Candi-
m
da
m)
n=9)
23,6±1,58*
23,6±1,14
22,6±0,55
19,8±1,48*
23,2±1,48
17,6±0,55
21,6±0,55*
14,2±1,92*
19,8±0,45
26,8±1,45
21,0±1,22
19,4±0,89*
30,8±1,48
24,8±1,92
24,4±1,61
23,0±1,0
25,2±1,48
17,6±0,45
25,4±2,07
17,6±1,14
21,0±1,22
27,5±1,9
22,8±0,84
21,6±1,14
n=7)
23,9±1,45
22,4±0,55*
26,4±0,89*
18,2±1,3*
20,4±1,34
28,8±1,79
29,6±1,52
29,6±1,07*
24,5±1,75
25,8±1,3
28,8±0,84
21,0±1,0
23,0±2,24
30,4±1,52
31,4±1,14
32,8±1,64
*-
–
21
-
4
639
–
–
Candida
Staphylococcus s
–
Candida
m)
14,4±0,55*
18,2±0,48*
22,8±0,84*
16,4±1,44*
20,6±0,55*
26,6±1,82*
16,2±0,84
20,0±1,0
27,4±1,22
21,4±2,88
23,8±1,3
30,4±0,44
*-
.
(1:8192 – 1:4096).
E.coli ATCC 2
[21].
E.coli
S.aureus
S.epidermidis
-16
-
22
- 1:2 1:4
S.aureus
ATCC
25923
S.aureus
(n=4)
S.epider
midis
(n=4)
E.coli
ATCC
25922
E.coli
(n=6)
CL
CL
CL
CL
CL
CL
CL
CL
CL
CL
CL
CL
CL
CL
CL
CL
CL
CL
CL
SCL
–
–
CL –
1:8 1:16 1:32 1:64 1:128 1:256 1:512 1:1024 1:2048 1:4096 1:8192 1:16384 1:32768
CL CL CL CL
CL CL CL SCL
CL CL CL CL
CL CL CL SCL
CL CL CL SCL
CL SCL SCL +++
CL CL CL CL
CL SCL SCL +++
CL CL SCL +++
+++ +++ ++ +
SCL –
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SCL
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SCL
SCL
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CL
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SCL
+
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SCL
++
SCL
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SCL
+
+
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–
SCL
++
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+
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2.
-
[17].
1.
3.
-
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Staphylococcus s
Candida
1.
2.
3.
4.
5.
6.
-
- 2001. - 4 (12). –
- 2000. -
-
//
-93.
-
-763.
- 2003. -
23
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-117.
-22.
-
-7.
- 2003. -
-
7.
8.
9.
4
639
2003. -17.
Adamsson I., Nord C.E., Lundquist P. et al. // J. of Antimicrob. Chemother. - 1999. - Vol.44. - P.629-640.
-2
10.
- 1999. - 4. -65.
11. Yoshimura T, Shimoyama T, Tanaka M. et al. // J. Clin. Pathol. - 2000. - Vol. 53. - . 532-536.
12.
.
–
–
13.
Helicobacter pylori
. - 1995. 14.
- 2003. -10.
15.
- 2004. -47.
16. Park
Giard J.-C., Eom J. et al. // J. of Bacteriology. - 1999. - Vol.181. - . 2. - . 689-694.
17.
1992. - 29-59.
18.
- 1984. - 873. - 147-189.
19.
20.
-126.
21.
- 2002. 4. -46.
. -
-
SENSITIVITY OF MICROORGANISMS TO ETIOTROPIC MEDICINES
AFTER MICROAEROPHILIC CULTIVATION’S CONDITIONS
N.I. Sklyar
I.I. Mechnikov Institute of Microbiology and Immunology of AMS of Ukraine, Kharkov
SUMMARY
The effect of microaerophilic conditions of cultivation on sensitivity of microorganisms to etiotropic medicines has been studied. It was shown that antibiotic resistance of bacteria and yeast-like fungi of Candida
genus increased after effect of above-mentioned factor. It was found that yield sensitivity changed to
-like fungi of
C
efined
(30,0±10,0) % of Candida strains in relation to amphotericin. Antibiotic sensitivity of selected objects to
cefalosporins of different generations was shown to be unchanged. It was found that lysis activity of specific
bacteriophages in relation to staphylococci and escherichia coli was 4-32-fold increased.
KEY WORDS: cultivation of microorganisms, microaerophilic conditions, antibiotic sensitivity,
bacteriophages
24