BY2012 Microbiology
Gallery of Candida
Candida Art
Candida albicans
Diagram as a budding yeast
Candida albicans and Oral Thrush
Source: Atlas of Clinical Oral Pathology, 1999
Candida albicans and Oral Thrush
Fungal Dimorphism
Candida albicans
Yeast phase – normal
flora [Gram stain]
Hyphal phase infection
Yeasts growing at 30°C on agar. Filamentous hyphae produced
when growing at 37°C in vivo causing invasion of epithelial tissue.
Candida – Buccal Epithelial Cells
Yeast cells
Epithelial cell
Hyphae
Candida albicans Infection
nucleus
epithelial cell
hypha
blastospores
Gram-stain of vaginal smear showing Candida albicans and
epithelial cells with many Gram-negative rods in background
Candida albicans
Scanning electron micrograph of C. albicans
germ tube penetrating living tissue (arrowed)
Candida albicans
Pseudohypha
Budding yeast
Phase contrast micrographs of C. albicans
Candida albicans
Wet mount - phase contrast
microscopy
Gram stain
Candida albicans
Stained with fluorescein-labelled antibodies – UV microscopy
Candida – Features
Budding yeast – Wet mount
Budding yeast – Gram stain
Germ tube
Chlamydospores
Candida
Pseudohyphae Production
Candida –Adherence to Buccal
(Cheek) Epithelial Cells
Candida – Cell Surface Features
Negative staining
Freeze etching
The outer cell wall layer is composed mainly of mannoproteins
covalently bound to ß-1,6- or ß-1,3-glucan and appears as a
dense network of radially projecting fibrils
Candida Dimorphism
Germ Tubes of Candida albicans
Colourised scanning electron micrograph of
C. albicans yeast cells producing germ tubes
Candida albicans forming
Hyphae on Corn Meal Agar
Yeast cells
Hyphae
Chlamydospores of
Candida albicans (low power)
Chlamydospores of
Candida albicans
Black arrows = Chlamydospores; White arrows= Suspensor cells
Chlamydospores of
Candida albicans
A
B
White arrows = Chlamydospores
Black arrows = Suspensor cells
C
Chlamydospores of
Candida albicans
Hypha
Chlamydospores
Stained with Gram’s stain
Chlamydospores of
Candida dubliniensis
Note multiple chlamydospores on the tips of the suspensor cells (arrowed)
Chlamydospores of
Candida dubliniensis
P = Pseudohypha; S = Suspensor cell
Chlamydospores occur in groups (arrowed)
Candida Identification
BBL CHROM Candida Agar
Candida Identification
BBL CHROM Candida Agar
BBL CHROM Candida Agar
C. dubliniensis
C. tropicalis
C. glabrata
C. albicans
C. parapsilopsis
C. krusei
BBL CHROM Candida Agar
C. albicans
C. krusei
C. tropicalis
C. glabrata
Candida Identification
HARDY CHROM Candida Agar
Candida glabrata
Candida krusei
Candida albicans
Candida tropicalis