Inertsil ODS-EP Technical Information
Advantages of Inertsil ODS-EP
● The selectivity is completely different from those of conventional
columns such as ODS column due to its specific polar group in the
stationary phase.
● Durability can be obtained by the high density bonding.
● Superior peak shapes of both acids and bases can be obtained because of
neutral bonded phase and low residual silanol groups.
● Silica gel of high-purity is used as the support material, which
minimizes effects of any remaining metal ions.
● Specifications of Inertsil ODS-EP
Support Silica Gel
Partical
Shape
diameter
Inertsil ODS-EP
5μｍ
Spherical
Chemical Modification
Specific
surface area
Pore
Purity
diameter
450ｍ ２ /g
100Å
99.999
Bonded group
Embededpolar
Octadecyl group
ODS Carbon
content
９%
(1) Naphthalene sample
~ for measurement retention and peak shape ~
Conditions
Column dimensions:
4.6 mm I.D x 150 mm length
Mobile phase:
Acetonitrile : Water =65 : 35
Flow rate: 1 ml / min
Column temperature: 40℃
Detection: UV 254 nm
1
2
4
3
0
2
4
Time (min)
Inertsil ODS-EP
6
8
Peak identification
1. Acetophenone
2. Benzene
3. Toluene
4. Naphthalene
Comparison of retention between Inertsil ODS-EP and commercial
embedded columns
0
2
4
6
0
2
Time (min)
Inertsil ODS-EP
0
2
4
6
0
2
4
6
Time (min)
Symmetry Shield RP18
Zorbax Bonus-RP
0
2
6
Discovery RP Amide C16
Time (min)
0
4
Time (min)
4
6
2
4
6
Time (min)
Time (min)
Xterra RP18
Hypersil HyPURITY Advance
Comparison of retention between Inertsil ODS-EP and other type
commercial columns
0
2
4
6
Time (min)
Inertsil ODS-EP
0
2
4
Time (min)
6
8
0
2
2
4
6
Inertsil C8-3
Inertsil ODS-3
0
4
Time (min)
6
Time (min)
Aqua C18(polar endcapping)
0
2
4
Time (min)
Inertsil Ph-3
6
(2) Selectivity test sample
~ for measurement selectivity ~
Conditions
Column dimensions:
4.6 mm I.D x 150 mm length
Mobile phase:
Acetonitrile : Water = 70 : 30
Flow rate: 1 ml / min
Column temperature: 40℃
Detection: UV 265 nm
Peak identification
1. Ethylbenzene
2
1
3
4
C2H5
2. Naphthalene
5
0
2
4
6
Time (min)
Inertsil ODS-EP
8
10
3. n-Propylbenzene
( CH2) 2 CH3
4. n-Butylbenzene
( CH2) 3 CH3
5. Anthracene
Comparison of selectivity between Inertsil ODS-EP and commercial
embedded columns
n-Butylbenzene
n-Butylbenzene
+ Anthracene
Anthracene
0
2
4
6
8
10
Time (min)
0
2
4
Time (min)
6
8
Discovery RP Amide C16
Inertsil ODS-EP
n-Butylbenzene
+ Anthracene
n-Butylbenzene
Anthracene
0
2
4
Time (min)
6
8
0
2
Symmetry Shield RP18
4
Time (min)
n-Butylbenzene
Anthracene
2
4
Time (min)
6
Xterra RP18
8
Zorbax Bonus-RP
n-Butylbenzene
0
6
Anthracene
8
0
2
4
Time (min)
6
8
Hypersil HyPURITY Advance
Comparison of selectivity between Inertsil ODS-EP and other type
commercial columns
n-Butylbenzene
Anthracene
0
2
4
6
8
10
Time (min)
Inertsil ODS-EP
Anthracene
n-Butylbenzene
Anthracene
n-Butylbenzene
0
0
2
4
6
Time (min)
8
10
2
4
Time (min)
12
Anthracene
n-Butylbenzene
n-Butylbenzene
Anthracene
0
2
4
6
Time (min)
8
8
Inertsil C8-3
Inertsil ODS-3
0
6
10
Aqua C18(polar endcapping)
12
2
4
Time (min)
6
Inertsil Ph-3
8
k' (n-Butylbenzene)
Comparison of retention and selectivity between Inertsil ODS-EP
and commercial embedded columns
10.00
9.00
8.00
7.00
6.00
5.00
4.00
3.00
2.00
1.00
0.00
0.80
Inertsil ODS-EP
Inertsil ODS-EP
Symmetry Shield RP18
Xterra RP18
Discovery RP Amide C16
Zorbax Bonus-RP
Hypersil HyPURITY Advance
Inertsil ODS-3
Inertsil C8-3
Inertsil Ph-3
Aqua C18
1.00
1.20
1.40
α（Anthracene/Butylbenzene）
The selectivity of Inertsil ODS-EP is completely different from
those of conventional columns such as ODS column due to its
specific polar group in the bonded phase.
(3) Tanaka-test sample
~ for measurement selectivity, hydrogen bonding capacity
and hydrophobicity ~
1
2
4
3
0
5
7
6
10
20
Time (min)
Inertsil ODS-EP
30
Conditions
Column dimensions:
4.6 mm I.D x 150 mm length
Mobile phase:
Methanol : Water = 80 : 20
Flow rate: 1 ml / min
Column temperature: 40℃
Detection: UV 254 nm
Peak identification
1. Uracil
2. Caffeine
3. Phenol
4. n-Butylbenzene
5. n-Amylbenzene
6. o-Terphenyl
7. Triphenylene
Comparison of selectivity between Inertsil ODS-EP and commercial
embedded columns
１
１
２
４ ５
３
0
７
７
６
10
20
Time (min)
２
３
４６５
30
0
2
4
6
8
10
Time (min)
Discovery RP Amide C16
Inertsil ODS-EP
１
１
２
３
７
４ ６
７
５
0
２
３
10
Time (min)
20
0
2
４６５
4
6
8
10
Time (min)
Symmetry Shield RP18
Zorbax Bonus-RP
１
１＋２
７
５６
２ ４
３
0
2
4
6
７
8
５+６
４
３
10
Time (min)
0
2
4
6
8
10
Time (min)
Xterra RP18
Hypersil HyPURITY Advance
Comparison of selectivity between Inertsil ODS-EP and other type
commercial columns
１
２
３
４ ５
0
10
７
６
20
Time (min)
30
Inertsil ODS-EP
１
１
７
２
４
３
２
６ ５
10
Time (min)
４
2
4
6
8
Time (min)
12
１
６+７
７
２
４
３
4
6
8
Time (min)
４５
３ ２
６５
10
14
Inertsil C8-3
１
2
10
20
Inertsil ODS-3
0
７
３
0
0
５+６
12
14
Aqua C18(polar endcapping)
0
2
4
6
8
10
Time (min)
Inertsil Ph-3
Characterization of Inertsil ODS-EP and commercial embedded
columns by Tanaka test
Hydrogen bonding capacity
α（caffeine/phenol）
0.50
0.40
0.30
0.20
0.10
(1)
0.00
Inertsil ODS-EP
Symmetry Shield
RP18
Xterra RP18
Discovery RP
Amide C16
Conditions
Column dimensions:
4.6 mm I.D x 150 mm length
Mobile phase:
Methanol : Water = 80 : 20
Flow rate: 1 ml / min
Column temperature: 40℃
Detection: UV 254 nm
Zorbax Bonus-RP Hypersil HyPURITY
Advance
Ｏ　Ｃ Ｈ３
(1) Caffeine shows no retention
Ｎ
Ｏ　Ｎ
Hydrophobicity
α（amylbenzene/butylbenzene）
ＯＨ
CH3　Ｎ
N
CH3
1.50
Caffeine
Phenol
1.00
0.50
（ Ｃ Ｈ２）
0.00
Inertsil ODS-EP
Symmetry Shield
RP18
Xterra RP18
Discovery RP
Amide C16
Zorbax Bonus-RP
Hypersil
HyPURITY
Advance
３
ＣＨ３
n-Butylbenzene
（ Ｃ Ｈ２）
４
CＨ ３
n-Amylbenzene
α（triphenylene/o-terphenyl）
Steric selectivity
3.00
2.50
o-Terphenyl
2.00
1.50
1.00
0.50
0.00
Inertsil ODS-EP
Symmetry Shield
RP18
Xterra RP18
Discovery RP
Amide C16
Zorbax Bonus-RP
Hypersil
HyPURITY
Advance
Triphenylene
(4) Pyridine-Phenol sample
~ for measurement of inertness to basic compounds ~
Conditions
Column dimensions:
4.6 mm I.D x 150 mm length
Mobile phase:
Methanol : 10mM Phosphate buffer(pH6.8)
=30 : 70
Flow rate: 1 ml / min
Column temperature: 40℃
Detection: UV 254 nm
2
3
1
0
2
4
6
8
Time (min)
10
Inertsil ODS-EP
12
14
Peak identification
1. Uracil
2. Pyridine
3. Phenol
Comparison of inertness to pyridine between Inertsil ODS-EP and
commercial embedded columns
Pyridine
Pyridine
Phenol
Phenol
0
2
4
6
8
Time (min)
10
12
14
0
0
2
4
8
10
0
2
12
6
Time (min)
8
10
12
0
2
4
6
Time (min)
8
10
12
Zorbax Bonus-RP
Pyridine
Phenol
4
8
Phenol
Symmetry Shield RP18
Pyridine
6
Time (min)
Pyridine
Phenol
6
Time (min)
4
Discovery RP Amide C16
Inertsil ODS-EP
Pyridine
2
Phenol
10
Xterra RP18
12
0
2
4
6
Time (min)
8
10
12
Hypersil HyPURITY Advance
(5) Acid test sample
~ for measurement of inertness to acidic compounds ~
Conditions
Column dimensions:
4.6 mm I.D x 150 mm length
Mobile phase:
0.1% Phosphoric acid(pH2.2)
Flow rate: 1 ml / min
Column temperature: 40℃
Detection: UV 210 nm
1
2
1.0
2.0
3.0
Time (min)
Inertsil ODS-EP
4.0
5.0
Peak identification
1. Formic acid
2. Acetic acid
Comparison of inertness to acids between Inertsil ODS-EP and
commercial embedded columns
Formic acid
Formic acid
Acetic acid
1.0
2.0
3.0
Time (min)
Acetic acid
4.0
1.0
5.0
2.0
3.0
Time (min)
4.0
5.0
Discovery RP Amide C16
Inertsil ODS-EP
Acetic acid
Acetic acid
Formic acid
1.0
2.0
3.0
Time (min)
4.0
5.0
1.0
Symmetry Shield RP18
2.0
3.0
Time (min)
4.0
5.0
Zorbax Bonus-RP
Acetic acid
Formic acid
+Acetic acid
Formic acid
1.0
1.0
2.0
3.0
Time (min)
Xterra RP18
4.0
5.0
2.0
3.0
Time (min)
4.0
5.0
Hypersil HyPURITY Advance
(6) Oxine-copper sample
~ for measurement of inertness to chelating compounds ~
Conditions
Column dimensions:
4.6 mm I.D x 150 mm length
Mobile phase:
Acetonitrile : 0.1% Phosphoric acid(pH2.2)
=10 : 90
Flow rate: 1 ml / min
Column temperature: 40℃
Detection: UV 254 nm
1
Peak identification
1. Oxine-copper
1.0
2.0
Time (min)
Inertsil ODS-EP
3.0
Comparison of inertness to oxine-copper between Inertsil ODS-EP and
commercial embedded columns
1.0
2.0
Time (min)
3.0
Inertsil ODS-EP
1.0
2.0
Time (min)
3.0
Symmetry Shield RP18
1.0
2.0
Time (min)
Xterra RP18
3.0
1.0
2.0
Time (min)
3.0
Discovery RP Amide C16
1.0
2.0
Time (min)
3.0
Zorbax Bonus-RP
1.0
2.0
Time (min)
Hypersil HyPURITY Advance
Relative retention time of
naphthalene（％）
Comparison of durability among Inertsil ODS-EP, ODS column
and Amide column
100
80
60
40
Inertsil ODS-EP
ODS test column
20
Amide test column
0
0
50
100
Flow time of 0.1% Phosphoric acid (pH2) ( hours )
Test condition
Column: 4.6 mm I.D. x 150 mm
Flow rate: 1 ml / min
Column temperature: 30℃
Inertsil ODS-EP
● The selectivity is completely different from those of conventional
columns such as ODS column, but the retention is close to conventional
columns.
Inertsil ODS-EP is useful for the samples which are not separated by
conventional columns.
● High durability can be obtained even if strong acid was used as mobile
phase.
Inertsil ODS-EP is useful for the LC/MS analysis.
● Superior peak shapes of both acids and bases can be obtained because of
neutral bonded phase and low residual silanol groups.
● Silica gel of high-purity is used as the support material, which
minimizes effects of any remaining metal ions.