Supplementary material S1. Comparison of MASP-­‐1/C1-­‐INH and MASP-­‐1-­‐an=-­‐
thrombin complexes in different plasma types. Error bars indicate SEM of triplicates. MASP-­‐1/AT complexes P<0.0001* Supplementary material S2. MASP-­‐1/an=thrombin complex levels in the HAE pa=ent cohort and the controls. *Non-­‐parametric, two-­‐tailed t-­‐test (Mann-­‐Whitney U-­‐test) Fic3 def serum Normal serum MBL def serum
Fic3 def serum Normal serum 150 kD MBL def serum 250 kD 100 kD 75 kD 50 kD Ficolin 3 37 kD MBL 25 kD 20 kD Supplementary material S3 PRMs represented by MBL and Ficolin-­‐3 with the MASP-­‐1/C1INH complexes. Normal human serum and two individual sera deficient in MBL or Ficolin-­‐3 incubated on the MASP-­‐1/C1-­‐INH protocol and analyzed by reducing SDS-­‐PAGE/immunoblo\ng with monoclonal an=bodies to either MBL or Ficolin-­‐3.