Version: 1
Revision Date: 11.30.2012
Purified anti-cdc2 (p34) Antibody
Catalog# / Size
626901 / 25 µg
Clone
POH-1 (See other available formats)
Other Names
Cell division cycle 2, G1 to S and G2 to M; cyclin
dependent kinase 1, p34 kinase, CDK1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Description
Cdc2 (p34), also known as cell division cycle 2, G1 to S and G2 to M; cyclin
dependent kinase 1, p34 kinase, and CDK1, is serine/threonine kinase that is
ubiquitously expressed in eukaryotes. Cdc2 is found as both a cytoplasmic
and nuclear protein. cdc2 is a catalytic subunit of a protein kinase called the
M-phase promoting factor that induces entry into mitosis universally in
eukaryotes. The cdc2 protein is highly phosphorylated on threonine, serine,
and tyrosine; tyrosine phosphorylation is subject to cell cycle regulation.
Cdc2 has been shown to interact with numerous proteins including cyclin
A1, cyclin A2, cyclin B1, cyclin B2, BRCA1, dynamin 2, Fyn, Lyn, and p53.
The POH-1 antibody recognizes human, monkey and bovine cdc2 and is
useful for Western blotting. The POH-1 antibody has also been reported to
be useful for immunoprecipitation, immunohistochemistry (formalin-fixed,
paraffin embedded), and immunofluorescence.
Hela extract was resolved by electrophoresis,
transferred to nitrocellulose and probed with
monoclonal antibody against cdc2 (p34).
Proteins were visualized using a goat antimouse secondary conjugated to HRP and a
chemiluminescence detection system.
Reactivity
Hela cells stained with purified mouse
monoclonal antibody against Cdc2(p34)
(clone POH-1), followed by Rhodamine RedX conjugated goat anti-mouse IgG and DAPI
Human, Monkey, Cattle (Bovine). Does not react with Mouse, rat, Xenopus, Drosophila
Immunogen
Recombinant human cdc2 protein
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.4, containing 0.09% sodium
azide at 0.5 mg/ml.
Preparation
This antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application
WB - Quality tested
IP, IHC , IF - Reported in the literature
Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting,
suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For
immunofluorescence microscopy: Use a dilution range of 1-4 µg/ml. It is recommended that the
reagent be titrated for optimal performance for each application.
Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry1 of
formalin-fixed, paraffin-embeded tissue sections, immunoprecipitation, and immunofluoresence
microscopy.
Application References
1. Lukas J, et al. 1992. Eur. J. Biochem. 207:169.
2. Liu Y, et al. 2014. J Control Release. 192:114. PubMed
(PubMed link indicates
BioLegend citation)
Publication Library
RRID
AB_2074779 (BioLegend Cat. No. 626901)
Antigen Details
Structure
Serine/threonine kinase; molecular weight approximately 34 kD
Distribution
Ubiquitous expression in eukaryotes, cytoplasmic and nuclear.
Function
cdc2 is a catalytic subunit of a protein kinase called the M-phase promoting factor that induces
entry into mitosis universally among eukaryotes.
Interaction
Interacts with numerous proteins including cyclin A1, cyclin A2, cyclin B1, cyclin B2, BRCA1,
dynamin 2, Fyn, Lyn, p53
Modification
Highly phosphorylated (threonine, serine, tyrosine); tyrosine phosphorylation subject to cell cycle
regulation
Antigen References
1. Draetta G, et al. 1988. Nature 336:738.
Gene ID
34411
UniProt
View information about cdc2 (p34) on UniProt.org
Documentation
Technical Data Sheet (pdf)
Certificate of Analysis
Safety Data Sheet
Related Protocols
Western Blotting Protocol
Related FAQs
There are no FAQs for this product.
Related Categories
Antibodies and More > Cell Biology > Protein Kinase/Phosphatase > Cdc2 (p34) >
Antibodies and More > Cell Biology > Antigens A-Z > cdc2 (p34) >
Antibodies and More > Cell Biology > Cell Cycle/DNA Replication > cdc2 (p34) >
Antibodies and More > Cell Biology > Signaling/Signaling Intermediates > cdc2 (p34) >
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