Global® for Fertilization
Product
Description:
Designed for standard IVF (coincubation of sperm and oocytes)
followed by culture of the
embryos in global® from Day 1
onward.
Catalogue
Number(s)
Unit Size
LGGF-020
20 ml
LGGF-050
50 ml
Intended Use
Why choose?
Composition
Storage Instructions
Shelf Life
Recommendations
Quality Control Specifications
Intended Use
Why would you choose
global For Fertilization is designed for
holding human cumulus-oocyte complexes
prior to conventional fertilization or ICSI,
and for conventional in-vitro fertilization.
global® for For fertilization to occur, a single sperm
Fertilization? must pass through the cumulus cells,
interact with the zona pellucida, fuse with
the oolemma and decondense inside the
ooplasm. The fertilization medium must
meet the metabolic requirements of the
sperm, cumulus cells, and oocyte.
Composition
Sodium
Chloride
Potassium
Chloride
Calcium
Chloride
Potassium
Phosphate
Magnesium
Sulfate
Sodium
Bicarbonate
Glucose
Lactate Na
Salt
Sodium
Pyruvate
Amino
Acids
EDTA
Gentamicin
Phenol Red
Storage Instructions Store at 2-8°C and protected from light.
Shelf Life No more than 8 weeks from the date of
release when stored unopened at 2-8ºC and
protected from light. For best results, use
within four weeks.
Recommendations for Use For a full and detailed recommended
protocol of use for global® medium
please contact us at
[email protected].
Quality Control Specifications
Specification
Common
Results
• pH (with
proper CO2
equilibration)
7.2-7.4
7.3
• Osmolality
260-270
265
Physiochemical
tests:
Biological
Tests
• Lal Endotoxin <0.50 EU/ml
<0.03
EU/ml
• Sterility Test,
membrane
Negative
filtration
Negative
1-cell Mouse
Embryo Assay
(% blastocysts >80%
at 96h of
culture)
90-100%
Precautions and Warnings
1. Not to be used for injection
2. Caution: Federal Law (USA)
restricts this device to sale by or on
the order of a physician (or
properly licensed practitioner).
3. Do not use the product if:
• the product packaging appears
damaged or if the seal is broken
• the expiry date has been exceeded
• the product becomes discolored,
cloudy, or shows evidence of
particulate matter
4. Record on the label: Date Received
and Date Opened
5. To avoid problems with
contamination, practice aseptic
techniques and discard minimal
amounts of excess medium
remaining in the bottle.
6. Once the bottle is opened, store the
product tightly capped at 2-8ºC and
protected from light.