DNA
RESEARCH
5, — Supplement, 147-155 (1998)
Complete Sequence and Gene Organization of the Genome of a
Hyper-thermophilic Archaebacterium, Pyrococcus horikoshii
OT3 (Supplement)
Yutaka KAWARABAYASI,1'2'* Mituhiro SAWADA, 1 Hiroshi HORIKAWA, 1 Yuji HAIKAWA, 1 Yumi HlNO,1
Saori YAMAMOTO, 1 Mitsuo SEKINE, 1 Sin-Ichi BABA, 1 Hiroki KOSUGI, 1 Akira HOSOYAMA, 1
Yoshimi NAGAI, 1 Mari SAKAI, 1 Keiko O G U R A , 1 Rie OTSUKA, 1 Hidekazu NAKAZAWA, 1 Minako TAKAMIYA, 1
Yuhko OHFUKU, 1 Tomomichi FUNAHASHI, 1 Toshihiro TANAKA, 1 Yutaka KUDOH, 1 Jun YAMAZAKI, 1
Norihiro KUSHIDA, 1 Akio OGUCHI,1 Ken-ichi AOKI, 1 Takio YOSHIZAWA, 1 Yoshinobu NAKAMURA, 1
Frank T. R O B B , 3 Koki HORIKOSHI,4 Yaeko MASUCHI, 5 Hiroaki SHIZUYA, 6 and Hisasi KlKUCHl1
National Institute of Technology and Evaluation, 2-49-10 Nishihara, Shibuya, Tokyo 151-0066, Japan,1
National Institute of Bioscience and Human-Technology, 1-1 Higashi, Tsukuba, Ibaraki 305-0046, Japan,2
Center of Marine Biotechnology, Columbus Center, University of Maryland, Biotechnology Institute,
Baltimore, MD, USA,3 Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka,
Kanagawa 237-0061, Japan,4 University of Tokyo, Meguro, Tokyo 153-0041, Japan,5 and California
Institute of Technology, Pasadena, CA, USA6
(Received 2 March 1998)
The circular genome of Pyrococcus horikoshii OT3,
(1,738,505 bp long), was opened at the junction of Srfl-C
and Srf I-E fragments, and is represented by a linear map starting from this site. The nucleotide positions are indicated on the linear map by numerals in
kb. Above and below the physical maps, the potential
protein-coding regions (ORFs) assigned are indicated by
boxes with arrowheads, indicating the directionof transcription. The detailed assignment procedures are described in the main article in this issue (pp. 55-76). The
results of ORF similarity and motif searches are shown
using the following color codes: red, similarities to reported genes with known functions; blue, similarities to
Communicated by Mituru Takanami
To whom correspondence should be addressed. Tel. +81-33481-8951, Fax. +81-3-3481-8424, E-mail: kyutakaOkazusa.
or.jp
hypothetical genes; green, some motifs but without significant similarity to the registered sequences; and no
color, no apparent similarity to any reported genes and
no significant protein motifs. The ORF names defined in the main article are given to each ORF, and
the positions of the rRNA and tRNA genes are indicated by closed box and vertical bars with a "T",
respectively. The sequence data reported here have
been deposited in DDBJ/Genbank/EMBL databases under accession numbers AP000001 (nucleotide positions
1-287,000), AP000002 (287,001-544,000), AP000003
(544,001-777,000),
AP000004
(777,001-994,000),
AP000005 (994,001-1,166,000), AP000006 (1,166,0011,485,000), and AP000007 (1,485,001-1,738,505).
148— Supplement
Complete Genome Sequence of P. horikoshii OT3
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