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A KEEN INSTINCT FOR WBC ABNORMALITIES:
Superior analytic performance and flagging efficiency decreases turnaround time
and provides guidance for diagnosis and therapy monitoring
The smear rate under
laboratory management’s control
Admission to hospital to clarify
an abnormal blood report
The parameter IG (immature granulocytes) already
enables many of our customers to significantly reduce
the number of smears – depending on the individual
threshold values.
The 3-dimensional DIFF flagging by the XN-Series
detects white blood cell abnormalities with great sensitivity – without compromises regarding specificity –
thanks to the special shape recognition of the subpopulation clusters, and delivers additional diagnostic
information, e.g. for infections.
10 DIFF PARAMETERS
INCLUDING IG%, #
3-DIMENSIONAL,
HIGHLY SENSITIVE FLAGGING
SPECIAL ‘LOW WBC’ MODE
FOR CRITICALLY LOW CELL COUNTS
Centralised
entralised QC
YOUR BENEFITS IN DAILY ROUTINE
Reru
erun
n & Refl
Reflex
ex
■
Reduced numbers of smears and therefore shortened turnaround times
enabled through the 3-dimensional flagging, while sensitivity and specificity
remain at the highest level.
■
Specifically during infections, the flagging message ‘atypical lymphocytes’ –
if triggered alone – can be matched directly with the proportion of
plasma cells present – this is helpful additional diagnostic information
for the treating physician.
NEUT %, NEUT #, LYMPH %, LYMPH #, MONO %, MONO #,
EO %, EO #, BASO %, BASO #, IG %, IG #
■
Fluorescence flow cytometry
The specially developed lysis reagent initially perforates the cell
membranes while leaving the cells largely native. The fluorescence marker labels the intracellular DNA and RNA in the second
step. The improved recipe of these two new reagent components
effects an even milder reaction with the blood cells, so that
almost all of the blood cell structure remains intact. Thus, even
better separation is achieved, particularly of the lymphocytes
and monocytes.
■
Flagging
In the scattergram, the cells are differentiated according to their
fluorescence signal and their internal structure. The intensity of
the fluorescence signal is directly proportional to the nucleic acid
content of the cell. Some of the strongest fluorescence signals
are shown by immature and activated cells, due to their high RNA
content, so that these are successfully detected and can even be
counted.
■
Adaptive cluster analysis system
(ACAS)
The flexible gating algorithm does not use rigid gating areas.
Instead it takes the biological variance into consideration when
evaluating the measured signals. Therefore, the results are
assessed individually, for example independently of the ethnic
origin or other characteristics of the patient.
■
Shape recognition analysis of the
WBC sub-population clusters
The very high sensitivity of this 3-dimensional WBC flagging is
based on the shape recognition analysis of the WBC clusters
in the scattergram. Consequently, abnormal shapes in the cell
clusters are now recognised precisely, in addition to the detection
of abnormal cells in the respective areas of the scattergram.
Especially in severe cases, IG supports the quick detection
of inflammations and infections as well as the monitoring
of later treatment.
Research parameters
High-fluorescence lymphocyte count = HFLC#, %,
counting antibody-secreting B-lymphocytes/plasma cells
(if haematological systemic diseases can be excluded)
Technology of WBC differentiation
SFL
IG
MONO
MONO
LYMPH
LYMPH
NEUT
IG
NEUT + BASO
EO
EO
SSC
FSC
Measurement modes
BASO
■
Special mode for low
white blood cell values
NRBC
BASO
NRBC
WBC
Ghost
PLT Clumps
SFL
For references to independent publications, please visit www.sysmex-europe.com/xn/literature or contact your local Sysmex representative.
Further specifications
■ Sample stability
A differential result can also be achieved in the pre-diluted mode,
in addition to the standard mode using whole blood.
Where necessary, samples with low WBC concentrations
(< 1,000 cells/µL) will be re-measured in the specific ‘Low WBC
mode’ as an automatic reflex test. With an increased counting
volume, the reliability of the WBC differentiation results
increases for all parameters.
up to 48 hours for the differential
www.sysmex-europe.com/xn
ZE000411.EN.N.09/12
Diagnostic parameters