Program Director/Principal Investigator (Last, First, Middle) : Gietz, Roman D.H.
BIOGRAPHICAL SKETCH
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NAME
POSITION TITLE
Roman Daniel H. Gietz
Associate Professor
eRA COMMONS USER NAME (credential, e.g., agency login)
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
DEGREE
(if applicable)
YEAR(s)
University of Guelph, Guelph, Ontario
B.Sc.
1978
Genetics
University of Alberta, Edmonton, Alberta
Ph.D.
1984
Genetics
INSTITUTION AND LOCATION
A.
FIELD OF STUDY
Personal Statement
I was trained in Genetics for both my degrees. I began my career studying Drosophila melanogaster,
however ended up moving to work in Saccharomyces cerevisisae, a very good genetic model for
studying eukaryotic mechanisms. My first years working in yeast made me realize that many of the tools
available at the time needed upgrading. This lead to my production of 9 unique Yeast: E. coli shuttle
vectors that allowed the utilization of the pUC18 multicloning site in an integrating, single copy and multi
copy cloning vehicle (Gietz RD and A. Sugino (1988) Gene 74:527-534). These vectors were requested
and sent to many labs worldwide and the paper describing them has been cited 2579 times in 4/16.
Continuing my study in yeast, I cloned and sequenced the yeast RAD4 DNA repair gene (Gietz RD and
S. Prakash (1988) Gene 74:535-541), however I also discovered and published the most efficient method
of DNA transformation of the time (Schiestl, R.H. and RD Gietz (1989) Curr Genetics 16:339-346). This
publication has been cited 1773 times in 4/16, however numerous of my subsequent publications on the
subject are also cited numerous time e.g. a) Gietz, D., A. St. Jean, R.A. Woods, and R.H. Schiestl (1992)
Nucl Acids Res 20:1425, has been cited 2652 times in 4/16, b) Gietz RD, R.H. Schiestl, A. Willems, and
R.A. Woods (1995) Yeast 11:355-360, has been cited 1379 times in 4/16, c) Gietz RD and R.A. Woods.
(2002) Methods in Enzymology. 350: 87-96, has been cited 1184 times in 4/16, d) Gietz RD and R.H.
Schiestl (1991) Yeast 7:253-263, has been cited 589 times in 4/16, e) Gietz RD, Schiestl RH. 2007. Nat
Protoc. 2(1):31-4, has been cited 408 times in 4/16.
With my expertise in transformation it made sense to apply this technique to other yeast methods
including the yeast two hybrid system (Y2H). Stan Fields, the father of the Y2H system, once told me
that the “TRAFO method made it possible to do screens with the Y2H system”. This lead to my
dabbling in Y2H screening and numerous collaborations between many different laboratories. In
addition, I published a number of “how to” papers for yeast two hybrid screening, a) Gietz RD,
B. Triggs-Raine, A. Robbins, K.C. Graham, and R.A. Woods (1997) Mol Cell Biochem 172:67-79, has
127 citations in 4/16. Finally, the last focus of my research interest has been to understand the
mechanism of DNA transformation in yeast. A mutant screen was initiated using a 96 well plate format
which uncovered numerous mutants that affected DNA transformation. In addition, the release of the
yeast knock out library, representing a collection of strains that contained amorphic mutants of every
viable open reading frame, made this approach more direct and further allowed the identification of
additional mutants that directly affect DNA transformation using the TRAFO method. Work is continuing
on this collection of mutants in an attempt to fully understand the complexities of how DNA is taken up
and established in the yeast cell.
0925-0001/0002 (Rev. 08/12)
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Biographical Sketch Format Page
Program Director/Principal Investigator (Last, First, Middle) : Gietz, Roman D.H.
B.
Positions and Honors
Positions and Employment
1984-1986
AHFMR Fellow, University of Alberta (with J.M. Tyler)
1986-1989
Consultant, Prairie Biological Research, Edmonton, Alberta
1986-1987
Visiting Fellow, NIH-NIEHS (with A. Sugino)
1987-1993
Scientist Consultant, GeneBioMed Inc.
1987-1989
Research Associate, University of Rochester (with S. Prakash)
1989-1996
Assistant Professor, Department of Human Genetics, University of Manitoba
1997-2003
Vice President Research, Molecular Research Reagents Inc.
1996-present Associate Professor, Department of Pediatrics and Child Health, University of Manitoba
1996-2000
Associate Professor, Department of Human Genetics, University of Manitoba
2000-present Associate Professor, Department of Biochemistry and Medical Genetics, University of Manitoba
2002-present Manitoba Medical Services Foundation Board of Directors
Awards and Honors
1978
Graduation with Honors in Genetics, University of Guelph
1980-1982
Alberta Heritage Foundation Medical Research Studentship
1983-1984
University of Alberta Dissertation Fellowship
1984-1986
Alberta Heritage Foundation Medical Research Postdoctoral Fellowship
1986-1987
Fogerty Foundation Visiting Fellowship NIH
1990-1993
Manitoba Health Research Council Scholar
1997-2000
Manitoba Medical Services Foundation Basic Science Scholarship
2002-2003
University of Manitoba Teaching Award (Faculty of Medicine)
C.
Selected Peer-reviewed Publications
1. Kraminsky, G.P., W.C. Clark, M.A. Estelle, RD Gietz, B.A. Sage, J.D. O'Connor, and R.B. Hodgetts (1980)
Induction of translatable mRNA for dopa decarboxylase in Drosophila: An early response to ecdysone.
PNAS 77:4175-4179.
2. Gietz RD and R.B. Hodgetts (1985) An analysis of dopa decarboxylase expression during
embryogenesis in Drosophila melanogaster. Dev Biol 107:142-155.
3. Hodgetts, R.B., W.C. Clark, D.D. Eveleth Jr., RD Gietz, C.A. Spencer, and J.L. Marsh (1986) Hormonal
aspects of the regulation of the dopa decarboxylase gene in Drosophila melanogaster. In: New
Discoveries and Techniques in Developmental Biology, Alan Liss, New York, Part A, pp. 221-234.
4. Spencer, C.A., RD Gietz and R.B. Hodgetts (1986) Developmental expression of the transcription unit
adjacent to the 3' end to the DDC gene in Drosophila melanogaster. Dev Biol 114:260-264.
5. Spencer, C.A., RD Gietz and R.B. Hodgetts (1986) Overlapping transcription units in the dopa
decarboxylase region of Drosophila. Nature 322:279-281.
6. Eveleth, D.D., RD Gietz, C.A. Spencer, F.E. Nargang, R.B. Hodgetts, and J.L. Marsh (1986) The
sequence and structure of the dopa decarboxylase gene of Drosophila: Evidence for novel RNA
splicing variants. EMBO J 5:2663-2672.
7. Gietz RD and A. Sugino (1988) New yeast - Escherichia coli shuttle vectors constructed with in vitro
mutagenized yeast genes lacking six-base-pair restriction sites. Gene 74:527-534.
8. Gietz RD and S. Prakash (1988) Cloning and nucleotide sequence of the Saccharomyces cerevisiae
RAD4 gene required for excision repair of UV damaged DNA. Gene 74:535-541.
9. Schiestl, R.H., RD Gietz RD Mehta, and P.J. Hastings (1989) Detection of carcinogens with the DEL
system in yeast. Carcinogenesis 10:1044-1053.
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Program Director/Principal Investigator (Last, First, Middle) : Gietz, Roman D.H.
10. Schiestl, R.H., W.S. Chan, RD Gietz RD Mehta, and P.J. Hastings (1989) Safrole, eugenol and
methyleugenol, reputed non-mutagenic carcinogens induce intrachromosomal recombination in yeast.
Mutation Research 224:427-436.
11. Schiestl, R.H. and RD Gietz (1989) High efficiency transformation of intact yeast cells using single
stranded nucleic acid as carrier. Curr Genetics 16:339-346.
12. Schiestl, R.H., RD Gietz, P.J. Hastings, and U. Wintersberger (1990) Interchromosomal and
intrachromosomal recombination in rad18 mutants of Saccharomyces cerevisiae. Mol Gen Genet
222:25-32.
13. Gietz RD and R.H. Schiestl (1991) Applications of high efficiency transformation of intact yeast cells
using single stranded nucleic acids as carrier. Yeast 7:253-263.
14. Kang, X., F. Yadao, RD Gietz, and B.A. Kunz (1992) Elimination of the yeast RAD6 ubiquitin conjugase
enhances base pair transitions and G:C-> T:A transversions as well as transpositions of the Ty
element: Implications for the control of spontaneous mutagenesis. Genetics 130:285-294.
15. Gietz, D., A. St. Jean, R.A. Woods, and R.H. Schiestl (1992) Improved method for high efficiency
transformation of intact yeast cells. Nucl Acids Res 20:1425.
16. Schiestl, R.H., P. Manivasakam, R.A. Woods, and RD Gietz (1993) Introducing DNA into yeast by
transformation. In: Methods: A Companion to Methods in Enzymology, eds. M. Johnston and S.
Fields, Academic Press, Inc., 5:79-85.
17. Roche, H., RD Gietz and B.A. Kunz (1994) Specificity of the yeast rev3∆ antimutator and REV3
dependency of the mutator resulting from a defect (rad1∆) in nucleotide excision repair. Genetics
137:637-646.
18. Gietz RD and R.A. Woods (1994) High efficiency transformation in yeast. Invited Book Chapter in:
Molecular Genetics of Yeast: Practical Approaches, ed. J.A. Johnston, Oxford University Press, pp.
121-134.
19. Paetkau. D.W., J.A. Riese, W.S. MacMorran, R.A. Woods, and RD Gietz (1994) Interaction between
the yeast Rad7 and Sir3 proteins: Implications for DNA repair and chromatin structure. Genes & Dev
8:2035-2045.
20. Gietz RD, R.H. Schiestl, A. Willems, and R.A. Woods (1995) Studies on the mechanism of high
efficiency transformation of intact yeast cells. Yeast 11:355-360.
21. Roche, H., RD Gietz and B.A. Kunz (1995) Mutator phenotypes conferred by inactivation of the
Saccharomyces cerevisiae RAD6, RAD18 and RAD52 genes exhibit different degrees of dependence
on the REV3 gene product, a putative nonessential DNA polymerase. Genetics 140:443-456.
22. Gietz RD, K.C. Graham and D.W. Litchfield (1995) Interactions between the subunits of casein kinase
II. J Biol Chem 270:13017-13021.
23. Gietz RD and R.H. Schiestl (1995) Transforming yeast with DNA. Meth Mol Cell Biol 5:255-269.
24. Litchfield, D.W., E. Slominski, S. Lewenza, M. Narvey, D.G. Bosc, and R. D. Gietz (1996) Analysis of
interactions between the subunits of protein kinase CK2. Biochemistry and Cell Biology 74:541-547.
25. Kalchman, M.A., R.K. Graham, G. Xia, H.B. Koide, J.G. Hodgson, K.C. Graham, Y.P. Goldberg, RD
Gietz, C.M. Pickart, and H.R. Hayden (1996) Huntingtin is ubiquitinated and interacts with a specific
ubiquitin conjugating enzyme. J Biol Chem 271:19385-19394.
26. Gietz RD, B. Triggs-Raine, A. Robbins, K.C. Graham, and R.A. Woods (1997) Identification of proteins
that interact with a protein of interest: Applications of the yeast two-hybrid system. Mol Cell Biochem
172:67-79.
27. Jerome, C.A., S.W. Scherer, L.-C. Tsui, RD Gietz, and B. Triggs-Raine (1997) Assignment of growth
factor receptor-bound protein 10 (GRB10) to human chromosome 7p11.2-p12. Genomics 40:215-216.
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Program Director/Principal Investigator (Last, First, Middle) : Gietz, Roman D.H.
28. Kalchman, M.A., H.B. Koide, K. McCutcheon, R.K. Graham, K. Nichol, K. Nishiyama, F.C. Lynn, P.
Kazemi-Esfarjani, M. Metzler, Y.P. Goldberg, I. Kanazawa, RD Gietz, and H.R. Hayden (1997)
Huntingtin and HIP1: A human homologue of S. cerevisiae Sla2p membrane associated partners in the
human brain. Nature Genetics 16:44-53.
29. Chen G., R. Ray, D. Dubik, L. Shi, J. Cizeau, R.C. Bleackley, S. Saxena, RD Gietz, and A.H.
Greenberg (1997) The E1B 19K/Bcl-2-binding protein Nip3 is a dimeric mitochondrial protein that
activates apoptosis. J Exp Med 186:1975-1983.
30. Meltzer, J.C., V. Sanders, P.C. Grimm, E. Stern, C. Rivier, S. Lee, S.L. Rennie, RD Gietz, A.K. Hole,
P.H. Watson, A.H. Greenberg, and D.M. Nance (1998) Production of digoxigenin- labeled RNA probes
and the detection of cytokine mRNA in rat spleen and brain by in situ hybridization. Brain Res Protoc
4:339-351.
31. Gietz RD and R.A Woods (1998) Transformation of yeast by the lithium acetate/single-stranded carrier
DNA/PEG method. In: Methods in Microbiology. Eds. A.J.P Brown and M.F. Tuite. Academic Press,
New York.
32. Gietz RD, R.A. Woods, P. Manivasakam, and R.H. Schiestl (1998) Growth and Transformation of
Saccharomyces cerevisiae. In Cells: A Laboratory Manual, Volume I. Culture and Biochemical
Analysis of Cells. Eds. D. Spector, R. Goldman and L. Leinwand. Cold Spring Harbour Press, Cold
Spring Harbour, New York.
33. Agatep, R., R.D. Kirkpatrick, D.L. Parchaliuk, R.A. Woods, and RD Gietz (1998) Transformation of
Saccharomyces cerevisiae by the lithium acetate/single-stranded carrier DNA/polyethylene glycol
(LiAc/ss-DNA/PEG) protocol. Technical Tips Online 1:51:P01525 (http://tto.trends.com).
34. Parchaliuk, D.L., R.D. Kirkpatrick, R. Agatep, S.L. Simon and RD Gietz (1999) Yeast two-hybrid
system: Part C-characterizing positives Technical Tips Online 1:69:P01714 (http://tto.trends.com).
35. Parchaliuk, D.L., R.D. Kirkpatrick, R. Agatep, S.L. Simon and RD Gietz (1999) Yeast two-hybrid system
screening: Part B-screening procedures. Technical Tips Online 1:68:P01713 (http://tto.trends.com).
36. Parchaliuk, D.L., R.D. Kirkpatrick, R. Agatep, S.L. Simon and RD Gietz (1999) Yeast two-hybrid system
screening: Part A-screen preparation. Technical Tips Online 1:66:P01616 (http://tto.trends.com).
37. Ray, R., G. Chen, C. Vande Velde, J. Cizeau, J.H. Park, J.C. Reed, RD Gietz, A.H. Greenberg (2000)
BNIP3 heterodimerizes with Bcl-2/Bcl-X(L) and induces cell death independent of a Bcl-2 homology 3
(BH3) domain at both mitochondrial and nonmitochondrial sites. J Biol Chem 275(2):1439-1448.
38. Bosc D. G., K.C. Graham, R.B. Saulnier, D. Prober, RD Gietz, and D.W. Litchfield (2000) Identification and
characterization of CKIP-1, a novel pleckstrin homology domain – containing protein that interacts with
protein kinase CK2; evidence for isoform-specific interactions with CK2. J Biol Chem 275(19):14295-306
39. Brown Gladden, J.G., R. Ray, RD Gietz, and M.R.A. Mowat (2000) Rapid screening of plasmid DNA by
direct sequencing from bacterial colonies. Biotechniques 29: 436-438.
40. Cizeau, J., R. Ray, G. Chen, RD Gietz, and A.H. Greenberg (2000) The C. elegans orthologue ceBNIP3
interacts with CED-9 and CED-3 but kills through a BH3 and caspase-independent mechanism. Oncogene.
19:5453-63.
41. Woods, R.A. and RD Gietz, (2000) Yeast Transformation. In: Introducing DNA into Living Cells and
Organisms. Eds; L. Steel and Norton, Eaton publishing, Natick, MA, Chapter 3 pp 25-43.
42. Hemming, R., R. Agatep, S. Kelly, K. Badiani, K. Wyant, G. Arthur, RD Gietz, and B. Triggs-Raine
(2001) Interaction of the human Grb14 adapter protein with the activated insulin Receptor. Biochem.
Cell Biol. 79:21-32.
43. Daly-Norquay, L., Y. Jin, R.M. Surabhi, RD Gietz, N. Tanese and P.A. Cattini, (2001) A member of the
nuclear factor-1 family is involved in the pituitary repression of the human placental growth hormone
genes. Biochem J. 354:387-395.
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Program Director/Principal Investigator (Last, First, Middle) : Gietz, Roman D.H.
44. Woods, R.A. and RD Gietz, (2001) High Efficiency Transformation. In: Methods in Molecular Biology:
Volume 177 Two hybrid systems: Methods and Protocols. Ed P. Macdonald. Humana Press, NJ. pp 8598.
45. Gietz RD and R.A. Woods. (2001) Genetic transformation of Yeast: BioTechniques 30:816-831.
46. Gietz RD and R.A. Woods. (2001) Screening for Protein-Protein interaction in the Yeast two hybrid
system. In: Embryonic Stem Cells: Methods and Protocols. Ed K. Turksen. Humana Press, Totowa NJ.
pp 471-486.
47. Gietz RD, and Woods RA. (2002) Screening for protein-protein interactions in the yeast two-hybrid
system. Methods Mol Biol: 185:471-86.
48. Gietz RD and R.A. Woods. (2002) TRANSFORMATION OF YEAST BY THE Liac/SS CARRIER
DNA/PEG METHOD. Methods in Enzymology. 350: 87-96.
49. Emberley ED, Gietz RD, Campbell JD, HayGlass KT, Murphy LC, and Watson PH. (2002) RanBPM
interacts with psoriasin in vitro and their expression correlates with specific clinical features in vivo in
breast cancer. BMC Cancer 2002 Nov 6;2(1):28
50. Emberley ED, Niu Y, Leygue E, Tomes L, Gietz RD, Murphy LC, Watson PH. (2003) Psoriasin interacts
with Jab1 and influences breast cancer progression. Cancer Res. 63(8):1954-61.
51. Malachi Griffith and RD Gietz. 2003. Escherichia coli endA deletion strain for use in two-hybrid shuttle
vector selection. BioTechniques 35:272-278.
52. Gietz RD, Woods RA. 2006. Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method. Methods Mol
Biol. 313:107-20.
53. Gietz RD 2006. Yeast two-hybrid system screening. Methods Mol Biol.313:345-72.
54. Truong D, Gietz, RD. 2007. Yeast transformation. Methods in Microbiology. 36:45-54.
55. Gietz RD, Schiestl RH. 2007. Frozen competent yeast cells that can be transformed with high efficiency
using the LiAc/SS carrier DNA/PEG method. Nat Protoc. 2(1):1-4.
56. Gietz RD, Schiestl RH. 2007. Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method.
Nat Protoc. 2(1):5-8.
57. Gietz RD, Schiestl RH. 2007. High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG
method. Nat Protoc. 2(1):31-4.
58. Gietz RD, Schiestl RH. 2007. Quick and easy yeast transformation using the LiAc/SS carrier DNA/PEG
method. Nat Protoc. 2(1):35-7.
59. Gietz RD, Schiestl RH. 2007. Large-scale high-efficiency yeast transformation using the LiAc/SS carrier
DNA/PEG method. Nat Protoc. 2(1):38-41.
60. G-L. Chee, B. Bhattrai, RD Gietz, S. Alrushaid, J.L. Nitiss, B.B. Hasinoff. 2012. Chemical reactivity and
microbicidal action of bethoxazin. Bioorganic and medical Chemistry Volume 20, Issue 4, 15 February
2012, Pages 1494-1501.
61. Gietz RD. 2014. Yeast transformation by the LiAc/SS carrier DNA/PEG method. Methods Mol Biol.
2014;1163:33-44. doi: 10.1007/978-1-4939-0799-1_4.
62. Donnard E, Queiroz EM, Ortega JM, Gietz RD. 2014. Yeast two-hybrid liquid screening. Methods Mol Biol.
2014;1163:97-107. doi: 10.1007/978-1-4939-0799-1_7.
63. Gietz RD. 2014. Yeast Transfomation by the LiAc/SS Carrier DNA/ PEG Method. Methods Mol Biol.
2014;1205;1-12. doi: 10.1007/987-1-4939-1363-3_1.
64. Day J, Gietz R D, Rampitsch C. 2015 Proteome changes induced by Pyrenophora tritici-repentis ToxA in
both insensitive and sensitive wheat indicate senescence-like signalling. Proteome Science 2015, 13:3
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Program Director/Principal Investigator (Last, First, Middle) : Gietz, Roman D.H.
D.
Research Support
There is no current funding at this time.
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