Control spoilage bacteria Description BactilessTM is a 100% natural non-GMO and non-allergenic biopolymer from fungal Aspergillus niger origin which helps to control the bacteria population in wines. BactilessTM formula helps to lower the viable acetic and lactic bacteria population allowing easy removal. Despite its effectiveness towards a wide spectrum of bacteria, BactilessTM does not affect yeast population. Its antibacterial effect can be enhanced with the use of SO2, but it does not replace it, as BactilessTM doesn’t have an antioxidant and antifungal effect. However, BactilessTM can help to reduce the amount of SO2 needed to control lactic and acetic bacteria population. BactilessTM helps to avoid the negative sensory impact caused by spoilage bacteria such as acetic acid and biogenic amines. Application and results • Microbiological stabilization action against : Lactic bacteria: BactilessTM can be used to drastically reduce bacteria population and to prevent bacteria growth in wines especially after malolactic fermentation offering an interesting alternative to lysozyme treatment and/or significant amounts of SO2. BactilessTM helps to protect wines from spoilage lactic bacteria and reduces their production of metabolites such as biogenic amines. Lactic bacteria management in red wines in Winery-scale trials. Lactic bacteria management in a red wine Spoilage bacteria contamination occurred during a stuck alcoholic fermentation (Malbec, Argentina, 2015) Lactic bacteria population (cfu/mL) 1.E+07 1.E+06 1.E+05 L. brevis, L. plantarum, L. paracasei, O. oeni Control 20 g/hL Lactobacillus 1.E+04 O. oeni 1,0E+05 1,0E+04 O. oeni 1,0E+03 1.E+03 1,0E+02 1.E+02 1.E+01 1.E+00 1,0E+01 Argentina Red wine – Stuck AF Spain – Merlot 2014 pH 3.84 – Free SO2 34 Total SO2 99 Spain – Syrah 2015 pH 3.81 – Free SO2 3 Total SO2 6 Spain – 2015 pH 3.57 – Total SO2 7 1,0E+00 Lactic bacteria Control 20 g/hL Lysozyme 20 g/hL Acetic bacteria management in red wines in Winery-scale trials. Acetic bacteria: BactilessTM is also effective against acetic bacteria helping to lower viable population and prevent their growth. This application can help to control volatile acidity levels. Acetic bacteria population (cfu/mL) 1.E+05 1.E+04 1.E+03 1.E+02 1.E+01 1.E+00 Argentina red wine 2015 Spain - merlot 2014 Spain - syrah 2015 Spain 2015 Control 20 g/hL France (Bordeaux) merlot 2015 Trial in a Chardonnay wine (pH = 3.4) in collaboration with IFV: Comparison of different microbial stabilization tools and kinetics of malic acid degradation in the case of a lactic acid bacteria contaminated wine. Malolactic fermentation control Malic acid degradation - chardonnay 2014 - pH 3.4 4,5 4,0 Malic acid (g/l) 3,5 Control 3,0 20 g/hL 2,5 SO2 - 4 g/hL Lysozyme 40 g/hL 2,0 1,5 1,0 0,5 0,0 0 10 20 30 40 50 60 70 80 90 In white and rosé wines, BactilessTM can help to delay or inhibit malolactic fermentation when it’s not desired. In red wines, BactilessTM can be used to delay the malolactic fermentation after treatment followed by racking. Time 100 (days) BactilessTM can avoid malolactic fermentation in white wines. Dosage and instructions for use • Recommanded average dosage from 20 g/hL up to 50 g/hL in case of high level contamination. • Suspend BactilessTM in water or wine before adding to the wine, then mix thoroughly the whole volume of tank. • Minimum contact time is 10 days. Then rack the wine and separate from its lees. Packaging and storage •1 0 x 500 g jars. • Store in a dry environment below 25° C. The information herein is true and accurate to the best of our knowledge however this data sheet is not to be considered as a guarantee expressed or implied or as a condition of sale of this product. May 2016 1480 Cader Ln. | Petaluma, CA 94954 ph. 707-765-6666 | fax 707-765-6674 [email protected] www.scottlab.com
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