BD Transduction Laboratories™
Technical Data Sheet
Purified Mouse Anti-KIF3A
Product Information
Material Number:
611508
Size:
50 µg
Concentration:
250 µg/ml
Clone:
28/KIF3A
Immunogen:
Human KIF3A aa. 563-671
Isotype:
Mouse IgG1
Reactivity:
QC Testing: Rat
Tested in Development: Human, Mouse
80/85 kDa
Target MW:
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium
azide.
Storage Buffer:
Description
The ability of the kinesin superfamily of motor proteins to hydrolyze ATP as they move progressively along microtubules is important for
organelle transport and cell division. Kinesins are grouped according to the location of the motor domain in the N-terminal, middle, or
C-terminal region of the protein. A family of N-terminal motor domain kinesin proteins includes KIF3A/3B, KRP85/95, and Klp68d/64.
These proteins form heterodimeric two-headed motors that interact with kinesin associated protein (KAP). The KIF3A/3B/KAP3
heterotrimeric motor has plus-end directed microtubule sliding activity. This heterotrimeric motor is expressed ubiquitously and is used for
anterograde transport of membranous organelles. KIF3A- and KIF3B-deficient mice have defects in the formation of the cilia located in the
embryonic node. In addition, KIF3A localizes to the basal body of the connecting cilium axoneme and to vesicles at the pre-synaptic ribbon in
photoreceptor cells. Thus, KIF3 motors are involved in microtubule-based formation and movement of cilia and the transport of synaptic
vesicles.
This antibody is routinely tested by the Western blot analysis. Other applications were tested at BD Biosciences Pharmingen during antibody
development only or reported in the literature.
Western blot analysis of KIF3A on rat brain lysate.
Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of
KIF3A.
Rat Neurons
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Store undiluted at -20° C.
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Application Notes
Application
Western blot
Routinely Tested
Immunofluorescence
Tested During Development
Product Notices
1.
2.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3.
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
4.
Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
References
Hirokawa N. Kinesin and dynein superfamily proteins and the mechanism of organelle transport. Science. 1998; 279(5350):519-526.(Biology)
Marszalek JR, Ruiz-Lozano P, Roberts E, Chien KR, Goldstein LS. Situs inversus and embryonic ciliary morphogenesis defects in mouse mutants lacking the
KIF3A subunit of kinesin-II. Proc Natl Acad Sci U S A. 1999; 96(9):5043-5048.(Biology)
Muresan V, Lyass A, Schnapp BJ. The kinesin motor KIF3A is a component of the presynaptic ribbon in vertebrate photoreceptors. Neuroscience. 1999;
19(3):1027-1037.(Biology)
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