AVANCE III HD
Fluorine-19 NMR Options
F NMR has seen an amazing revival in the recent past. The Bruker
Biospin Avance III HD console and probes are ideally equipped to
give you the highest sensitivity results on your fluorine containing
samples.
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Fig. 5 Clean spectra even in automation with 19F decoupled 1H spectra and vice versa.
No artefacts from environmental RF sources visible.
Fig.1 Examples of Probes with fluorine capability, QCI Cryoprobe left, BBFO Smart Probe
center, BBO Prodigy right
More Probes with 19F option
Bruker offers a wide variety of cryogenically cooled and
conventional probes that include the capability to observe Fluorine
spectra. Some probes contain a dedicated channel for fluorine,
in some probes the tuning range of the multinuclear channel
reaches 19F and in some probes the proton channel can be tuned
all the way to fluorine. For more complex experiments triple
resonance conventional probes or four channel CryoProbes are
also available.
Probe
BBFO Smartprobe
QXI (HFCN) Cryoprobe
Field (MHz)
400 – 700
500 – 800
Benefits
Applications
F{1H}, 1H{19F} and X{1H}, 1H{X} VT Range -150
to +180° C
Small Molecules and Polymers
F{1H}, 1H{19F} and full range of HFCN 4 channel experiments
Proteins, Nucleic Acids, Drug
Discovery
19
19
H/F/X and F/C/H RT probes
400 – 800
F{1H}, 1H{19F} and X{1H,19F}, 1H{19F,X} VT Range Small Molecules, Polymers,
-150 to +180°C
Proteins, Drug Discovery
TCI Cryoprobe
400 - 1000
1
Cryoprobe Prodigy
400 - 700
1
Applications:
Small molecules containing 19F
If small organic molecules containing fluorine are often found
amongst the samples you look at, but good heteronuclear
sensitivity is important then the BBFO SmartProbe is your best
choice. This probe will allow you to collect 19F spectra with proton
decoupling and vice versa but also any combination of proton
and another nucleus in the range of 31P – 109Ag.
Fluorinated polymers
For polymer work an extended high temperature range is
important. Cryoprobes are optionally available with a range of up
to 135°C, BBFO cryoprobe or HFCN QCI would be applicable
here. All room temperature probes can be brought up to 180°C.
For polymer applications the SmartProbe would again be the
ideal choice amongst the conventional probes
19
H Channel tunable to 19F (no 1H decoupling)*
Proteins, Nucleic Acids with
broad 19F signals
H Channel tunable to 19F (no 1H decoupling)*
Proteins, Nucleic Acids with
broad 19F signals
For simpler double resonance 1H/19F applications the high
frequency amplifier can be shared between protons and fluorine
with our high speed switching technology. For more complex
multichannel applications the use of dedicated amplifiers for each
of the nuclei is recommended.
Specialized preamplifiers allow optimal results with either
dedicated 19F channels and also with probes where the proton
channel is tunable to fluorine.
Drug Discovery
For this application there are two main approaches. One uses
small molecules that contain 19F, the other puts the fluorine on
the target. The first case usually requires 1H decoupling as the
signals of the small molecule are narrow. This requires a probe
with this capability. When fluorine is attached to a large molecule
the lines are broad and decoupling is not required, thus a probe
with the 1H channel tunable to 19F will be sufficient.
Summary
„„Largest
Fig.2 Modern HPLNA preamplifier technology with state of the art human interface and
well shielded NMR RF electronics. Insight of 1H - 19F HPLNA preamplifier.
portfolio of probes for 19F experiments
„„Highest sensitivity with 19F enabled cryoprobes
„„Advanced preamplifier and filter technologies for artefact
free spectra