ATCC Medium: 5080 Dryl’s Solution
Stock Solution A………………………………..10.0 ml
Stock Solution B………………………………..10.0 ml
Stock Solution C………………………………..20.0 ml
Stock Solution D………………………………..15.0 ml
DI Water……..…………………………………...945.0 ml
Aseptically add each stock solution to sterile distilled water in order listed to avoid
precipitation. Dispense as required.
Stock Solutions A
NaH2PO4 x H2O………………………………….1.380 g
DI Water…….…………………………………….100.0 ml
Stock Solutions B
Na2HPO4………………………………………….1.42 g
DI Water…………………………………………...100.0 ml
Stock Solutions C
Na3C6H5O7 x 2H2O (Sodium citrate)…………….2.941 g
DI Water……………………………………………100.0 ml
Stock Solutions D
CaCl2 x 2H2O……………………………………….1.470 g
DI Water……………………………………………..100.0 ml
Autoclave Solutions A through D separately at 121 C.