Datasheet GeneTex, Inc : Toll Free 1-877-GeneTex (1-877-436-3839) Fax:1-949-309-2888 [email protected] GeneTex International Corporation : Tel:886-3-6208988 Fax:886-3-6208989 [email protected] Date : 2016/4/12 Catalog Number Product Name GTX84003 Package:100 μl NRBP1 antibody [6C11] Full Name nuclear receptor binding protein 1 Synonyms NRBP, NRBP1, MUDPNP, BCON3, MADM Product Description Specificity Background Host Clonality Mouse monoclonal antibody [6C11] to NRBP1 GTX84003 is specific for human NRBP1. May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rhotype GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication. Mouse Monoclonal Clone Name 6C11 Isotype IgG2a Target NRBP1 Immunogen Antigen Species Species Reactivity Applications Application Note Protein expressed in 293T cell transfected with human NRBP1 expression vector Human Human, Monkey FACS, IHC, IHC-P, WB Recommended Starting Dilutions: WB 1:2000 IHC 1:50 Optimal working dilutions should be determined experimentally by the end user. Cellular Localization Form Supplied Purification Storage Buffer Storage Instruction Notes Colocalizes with activated RAC3 to endomembranes and at the cell periphery in lamellipodia. Liquid Purified from mouse ascites fluids by Protein A/G affinity chromatography PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. GTX84003 IHC-P Image Immunohistochemical staining of paraffin-embedded breast tissue using anti-NRBP1 mouse monoclonal antibody. (GTX84003, Dilution 1:50) GTX84003 IHC-P Image Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-NRBP1mouse monoclonal antibody. (GTX84003, Dilution 1:50) GTX84003 WB Image HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRBP1. GTX84003 WB Image Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-anti-NRBP1monoclonal antibody. GTX84003 WB Image Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NRBP1 monoclonal antibody. GTX84003 FACS Image HEK293T cells transfected with either NRBP1 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NRBP1 antibody (GTX84003), and then analyzed by flow cytometry.
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