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Supporting Information
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Salicylimine-based Fluorescent Chemosensor for Aluminum
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Ions and Application to Bioimaging
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Soojin Kim,1 Jin Young Noh,2 Ka Young Kim,1 Jin Hoon Kim,2 Hee Kyung Kang,1 Seong-Won
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Nam,1, So Hyun Kim,1 Sungsu Park,1,3* Cheal Kim,2* Jinheung Kim1*
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Department of Chemistry and Nano Science,
Ewha Womans University, Seoul, 120-750, Korea
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Department of Fine Chemistry,
Seoul National University of Science and Technology, Seoul, Korea
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Mechanobiology Institute, National University of Singapore, Singapore 117411
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[∗∗]
This work is supported by the National Research Foundation of Korea Grant funded by the
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Korea Government (2010-0009525), Korea Science & Engineering Foundation (2009-0074066),
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Converging Research Center Program through the National Research Foundation of Korea
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(NRF) funded by the Ministry of Education, Science and Technology (2011K000675), and BK21
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(to S.L., K.Y.K. and H.K.K.).
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150
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50
ppm
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Figure S1. 13C NMR spectrum of PSI in methanol-d4.
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0
-50
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(A)
0.6
Absorbance
0.5
0.4
0.3
0.2
0.1
0.0
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300
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500
wavelength (nm)
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(b)
0.6
Absorbance
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0.3
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0.1
0.0
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wavelength (nm)
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(c)
0.6
Absorbance
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0.2
0.1
0.0
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wavelength (nm)
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Figure S2. UV-visible spectral changes of 50 µM PSI after the addition of aluminum ions (0, 1,
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2, 3, 4, 5, and 6 equiv) in 10 mM phosphate buffer and CH3OH (98:2): (a) pH 5.0, (b) 6.0, and
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(c) 7.0.
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(a)
800
pH 6
pH 5
Intensity
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pH 7
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wavelength (nm)
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(b)
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Intensity
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pH
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Figure S3. (a) Fluorescence spectra of 10 µM PSI after the addition of 10 equiv aluminum ions
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in 10 mM phosphate buffer and CH3OH (98:2). (b) Plot of the fluorescence intensity of PSI at
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510 nm against pH upon addition of 10 equiv Al3+.
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9.0
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Figure S4. 1H NMR spectra of PSI with Al(ClO4)3 in methanol-d4: (a) PSI upon addition of 2
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equiv of Al3+ (bottom); (b) PSI upon addition of 4 equiv of Al3+ (top).
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Relative abundance
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250 260 270 280 290 300 310 320 330 340 350
m/z
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Figure S5. Positive ion electrospray ionization mass spectrum of PSI upon addition of Al(NO3)3
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(2 equiv) in CH3OH.
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