C H A P T E R Chapter Expectations Briefly explain each of the following points. • Metabolism involves the use of chemical energy. (3.1) • Through a series of reactions, photosynthesis transfers the energy in light to energy in chemical bonds. (3.2) • The chemical energy stored through photosynthesis is transferred to a form usable anywhere in the cell during cellular respiration. (3.2) • All eukaryotic and most prokaryotic cells use glycolysis to form ATP. (3.3) • Aerobic respiration efficiently produces 36 or 38 molecules of ATP from one molecule of glucose, but it requires that a cell have access to oxygen. (3.3) • Anaerobic respiration quickly forms two molecules of ATP per molecule of glucose, but produces toxic waste products. (3.3) • The structure of chloroplasts and mitochondria provides evidence that these organelles may once have been free-living micro-organisms. (3.4) 3 R E V I E W • Research into cellular biology has led to practical applications. (3.4) • Some industries harness the ability of bacteria to metabolize organic materials toxic to most organisms. (3.4) Language of Biology Write a sentence using each of the following words or terms. Use any six terms in a concept map to show your understanding of how they are related. • • • • • • • • • • • energy potential energy kinetic energy chemical energy metabolism wave model of light electromagnetic spectrum photon model of light photons pigments chlorophyll • • • • • • • • • • • • photosystems reaction centre ATP Calvin cycle cellular respiration aerobic glycolysis fermentation oxygen debt anaerobic bioremediation radioactive UNDE RSTAN DIN G CON CEPTS 1. What is the source of energy in your body? 2. Explain the terms “potential energy” and “kinetic energy” with respect to a cell. 3. (a) How do your muscle cells use energy? (b) Describe conditions where muscles use an alternative process. (c) Explain one possible reason that muscle cells are the best cells to switch to the alternative process. 4. Describe three metabolic processes in your body powered by energy in your food. 7. Write the simple reaction for photosynthesis. (a) Which molecule listed has the highest potential energy? Justify your response. (b) Which molecule has the lowest potential energy? Justify your response. 8. Describe the features of light energy that are important to the process of photosynthesis. 9. Identify one pigment used by a chloroplast to help with photosynthesis. 10. What colour or colours of light are absorbed by a green leaf? Explain your response. 5. “Energy is used to keep the cell organized.” (a) What does this statement mean? (b) What would happen to a cell that has no energy? 6. Refer to Chapter 1 to find the structural formula of glucose. Glucose is made of carbon, oxygen and hydrogen atoms. Identify the source of each type of atom in the process of photosynthesis. Cells, Energy, and Technology • MHR 107 11. Where in the chloroplast are the photosystems found? Draw a diagram and indicate the location of the photosystems. 12. Briefly describe the role played by each photosystem in the chloroplast. 15. What is the role of cellular respiration in your cells? 16. Draw a diagram of a cell and show where each stage of cellular respiration takes place. 17. How is oxygen important to cellular respiration? 13. Where do the synthesis reactions of photosynthesis take place? 18. Identify three different organisms that use fermentation regularly. 14. (a) List the three stages of photosynthesis. (b) Identify how the energy captured in one step is used to power changes in another step. 19. How are radioactive molecules used to study metabolic processes? IN QU IRY β-gal (µg) 20. Lactose is a sugar that most bacteria can use as a source of glucose. They can do so because they manufacture an enzyme called betagalactosidase (β-gal). This enzyme will break down a molecule of lactose into a molecule of glucose and a molecule of galactose. In an experiment, bacteria were supplied with lactose, and the amount of β-gal was monitored. The results are illustrated in the graph below. (a) What can be interpreted from the graph? (b) Why would it be incorrect to conclude from these results that lactose is acting directly on the transcription of mRNA to produce the β-gal protein? (c) Sketch a graph that would illustrate the relationship between cytoplasmic glucose and β-gal levels after the addition of lactose. 21. Dinitrophenol (DNP) is a chemical that interferes with the production of ATP in both chloroplasts and mitochondria. In an attempt to identify the minimal concentration of DNP required to inhibit ATP production, DNP was applied to separate suspensions of both organelles. Data are presented in the table. (a) Which set of data would represent the chloroplast? (b) Which organelle would be considered the most active? Explain. (c) Which organelle is most susceptible to changing concentrations of DNP? Explain. Concentration of DNP Organelle 5% 15% 25% 35% Volume of CO2 released (mL) 0.50 0.11 0.05 0.01 Volume of O2 released (mL) 0.88 0.04 0.01 n/a 22. Glycolysis takes place in the cytoplasm of eukaryotic cells. This indicates that the process is very ancient, probably older than the development of eukaryotic cells. Explain briefly. Lactose added 23. In single-celled organisms, compare the relative need for kinetic energy in a plant-like cell and an animal-like cell. total bacterial protein (µg) COMMU N ICATIN G 24. On Earth, matter cycles and energy flows. (a) To make this statement clearer, explain how matter is different from energy. (b) Draw a diagram to illustrate the given statement. 108 MHR • Cellular Functions 25. Make a concept map or flowchart that shows what happens to a glucose molecule from the time it enters a plant or animal cell. 26. Make a diagram showing how photosynthesis and aerobic respiration form a cycle of connected reactions. 29. A train has derailed 13 km from your home. Some toxic chemicals spilled onto the ground. Develop a bioremediation plan to share with your town council. Who could you contact for support and help? 30. Energy profiles for photosynthesis and cellular respiration are illustrated above. (a) With reasons to support your choices, identify which energy profile is for cellular respiration and which is for photosynthesis. (b) Compare the energy required to make glucose to the amount of energy required to break it down. B Potential Energy (kJ) 28. Refer to Chapter 2 for information about the chloroplast and the mitochondrion. (a) Draw a diagram of a chloroplast. Label your diagram to show the outer membrane, thylakoids, grana, stroma and lamellae. (b) Draw a diagram of a mitochondrion. Label your diagram to show the outer membrane, inner membrane, cristae and matrix. (c) Compare these two structures. Make a chart to record how the structures are similar and how they are different. A Potential Energy (kJ) 27. Review the process of phagocytosis from Chapter 1. Describe the structure of the vesicle formed. Compare this to the outer membrane of both the chloroplast and the mitochondrion. Time Time 31. Living cells capture a tiny amount of the solar radiation available. This tiny amount supports almost all the life on earth. (a) What would be the consequence if twice as much energy were captured? Explain. (b) What would be the consequence if half as much energy were captured? Explain. 32. Eukaryotic cells have mitochondria for aerobic respiration. Some eukaryotic cells have chloroplasts to capture solar energy in food molecules. Indicate whether you agree or disagree with the following statements: (a) The first cells with chloroplasts were predators. Explain briefly. (b) Cells with chloroplasts are still predators. Explain briefly. (c) Perhaps the biggest physical difference between a plant cell and an animal cell is the presence of a cell wall. Explain briefly. M A KIN G CON N ECTION S 33. Despite many attempts, researchers have been unable to get photosynthesis to proceed outside a chloroplast. Why are researchers still interested in pursuing this goal? What possible practical use could come from attaining the goal? 34. People have made use of fermentation for thousands of years. (a) Identify two ancient uses for this cellular process. (b) Identify one modern use for this cellular process. These people wanted to learn more about the biological processes in their industry. (a) Today, pasteurization is used in the milk industry, but not in the wine industry. Explain briefly. (b) Explain the relationship between the reproducing yeast cells shown here and the process of fermentation. 35. People now turn to bacteria for biological solutions of technological problems. Explain this using one example from the text. 36. In Chapter 1, you learned about the experiments conducted by Louis Pasteur to explain how micro-organisms can spoil a broth. This research was funded by the wine industry. Cells, Energy, and Technology • MHR 109
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