ATP6V0A2 Antibody
CATALOG NUMBER: 25-762
Antibody used in IHC on human kidney at
Antibody used in IHC on Human small
Antibody used in IHC on Human kidney at
5 ug/ml.
intestine at 5 ug/ml.
5.0 ug/ml.
Antibody used in WB on Human HeLa at
Antibody used in WB on Human Hela at:
Antibody used in IHC on Human Hela at
1:300.
1:300.
1:250.
Specifications
SPECIES REACTIVITY:
Human, Mouse, Rat
TESTED APPLICATIONS:
ELISA, WB
APPLICATIONS:
ATP6V0A2 antibody can be used for detection of ATP6V0A2 by ELISA at 1:312500. ATP6V0A2 antibody can be
used for detection of ATP6V0A2 by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be
diluted 1:50,000 - 100,000.
USER NOTE:
Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:
1) Cat. No. 1201 - HeLa Cell Lysate
PREDICTED MOLECULAR
98 kDa
WEIGHT:
IMMUNOGEN:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP6V0A2.
HOST SPECIES:
Rabbit
Properties
PURIFICATION:
Antibody is purified by peptide affinity chromatography method.
PHYSICAL STATE:
Lyophilized
BUFFER:
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 uL of distilled water. Final antibody concentration is
1 mg/mL.
CONCENTRATION:
1 mg/ml
STORAGE CONDITIONS:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATP6V0A2 antibody at 20˚C. As with any antibody avoid repeat freeze-thaw cycles.
CLONALITY:
Polyclonal
CONJUGATE:
Unconjugated
Additional Info
ALTERNATE NAMES:
ATP6V0A2, ATP6N1D, ATP6a2, J6B7, Stv1, TJ6, TJ6M, TJ6s, Vph1, a2, A2, RTF, WSS, ARCL, STV1, TJ6S,
VPH1, ARCL2A, ATP6A2
ACCESSION NO.:
NP_036595
PROTEIN GI NO.:
42741679
OFFICIAL SYMBOL:
ATP6V0A2
GENE ID:
23545
Background
BACKGROUND:
The multisubunit vacuolar-type proton pump (H (+)-ATPase or V-ATPase) is essential for acidification of diverse
cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and
chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H (+)ATPases are comprised of a peripheral V (1) domain and an integral membrane V (0) domain; ATP6V0A2 is a
component of the V (0) domain.The multisubunit vacuolar-type proton pump (H (+)-ATPase or V-ATPase) is
essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated
vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of
certain specialized cells. H (+)-ATPases are comprised of a peripheral V (1) domain and an integral membrane V
(0) domain; ATP6V0A2 is a component of the V (0) domain (Smith et al., 2003 [PubMed 14580332]).
REFERENCES:
1) Kornak, U., (2008) Nat. Genet. 40 (1), 32-34.
FOR RESEARCH USE ONLY
December 12, 2016