2010 American Control Conference Marriott Waterfront, Baltimore, MD, USA June 30-July 02, 2010 FrC08.5 Apply Tapping Mode Atomic Force Microscope with CD/DVD Pickup Head in Fluid Shih-Hsun Yen, Jim-Wei Wu, and Li-Chen Fu Abstract—This paper proposes a tapping mode scanning sample type Atomic Force Microscope (AFM) equipped with a CD/DVD pick-up-head (PUH) used to measure the deflection of the cantilever beam of the probe in the liquid. To start with, we build an adaptive Quality-Factor-controller (Q-controller) to modulate the interaction force between the tip and the sample. To implement the above systems, we have designed a novel AFM mechanism and proposed an adaptive sliding-mode controller for it. For testing the system capability and analyzing the biomorphic change of the sample in liquid, we have conducted a series of experiments, and the results can help us to understand more about the mechanism of the sample in liquid. I. INTRODUCTION Biological samples can be imaged with AFM in either of the two imaging modes: contact mode or tapping mode [1][2]. For AFM studies, biological materials are often both delicate and tenuously immobilized on a surface, even more so in fluid than in air. As a result, the vertical and shear forces exerted on the sample via the tip in contact mode can damage the sample by compressing, tearing, or removing it from the surface[3]. Applications of fluid tapping mode AFM [4] [5] in biology are constantly growing and the data obtained with this technique are improving, especially in terms of resolution. Even dynamic processes can be observed almost as they would occur in vivo [6]. So far, in the literature there exists an AFM which is developed using CD/DVD PUH [7] [8] [9]. A PUH is light enough to be carried for rapid scan so that the sample can remain fully stationary. Besides, a cost-effective, and lightweight system with good scanning performance will be needed for scientific development and is valuable in industrial applications. The goal of this research is to design an AFM with the aforementioned features. Utilizing an optical pickup device as the measuring system and developing an advanced feedback controller are the appropriate methods to realize such a system [10][11]. For the former, there still exists difficulty in employing CD/DVD PUH in liquid, namely, the measuring system will encounter refraction problem when the light has to go through different media, and hence a novel mechanical design has been hereby designed to solve this tough situation. As for the latter, this research build an adaptive Q control, for adjusting the Q S. H. Yen and J. W. Wu are with the Department of Electrical Engineering, National Taiwan University, Taipei, Taiwan, ROC. (e-mail:[email protected]) L. C. Fu is with the Department of Electrical Engineering and Department of Computer Science and Information Engineering, National Taiwan University, Taipei, Taiwan, ROC. (e-mail:[email protected]) This work was supported by National Science of Council under the grant NSC 98-2218-E-002-014. 978-1-4244-7425-7/10/$26.00 ©2010 AACC factor of a piezo-actuated bimorph probe dynamically during scanning when necessary [12][13][14]. Specifically, in our approach the Q factor of the probe is modified adaptively depending on the profile of the surface being scanned. There are four sections in this paper. Section II describes the system design from the viewpoint of hardware/software. Then, in section III adaptive Q control and adaptive sliding- mode control are proposed and analyzed to assure satisfactory operating performance of the designed AFM. Numerical simulation and extensive experiments are also provided in this chapter to validate our design. In the final section, we will make a conclusion summarizing the hereby obtained achievements. II. SYSTEM DESIGN A schematic diagram of the experimental setup of an AFM driven in the amplitude modulation mode (tapping mode) is shown in Fig. 1. The cantilever is driven at a fixed frequency by a constant sinusoidal signal originating from the lock-in amplifier, and the resulting oscillating amplitude is also detected by the lock-in amplifier. The PC-based controller we have designed will use that signal to adjust the z-scanner. The piezoelectric tube scanner is driven by a homemade high voltage amplifier, whose operation range is -200 V to +200 V. The scanning range is 35 µm × 35 µm × 9 µm in x-direction, y-direction, and z-direction, respectively. Figure 1. Schematic drawing of the experimental setup of the AFM system using the constant excitation mode. A PC-based controller is used to compensate the xy-trajectory and to reduce the tracking error in z-axis. Unlike other commercial AFM, we use CD/DVD PUH to detect the cantilever’s oscillation amplitude in this work. It is worthy to note that positions of all elements of the entire AFM setup may change due to heat or other factors, and therefore we should properly adjust our system to make sure the laser beam focuses on the tip. When we 6549 operate the AFM in liquid, the laser beam can be refracted or scattered and it’s hard to focus it at a focal point. As a result, we construct a mechanism to ensure the laser beam does focus on the tip without suffering from refraction or scattering. Besides, this mechanism can prevent movement of the laser spot when the scanner is moved upward and downward. The kernel element of the designed AFM system is the pickup head (as shown in Fig. 4), which is used to measure the cantilever deflection. As mentioned earlier, the relative positions of different components may change due to temperature variation or other factors, but the laser beam always needs to focus on the tip exactly. Besides, the probe may be worn away by scanning [15], thereby a precision tuning mechanism (see Fig. 2) needs to be incorporated to solve this problem. The mechanism includes a precision miniature linear stage, a piezoelectric element (bimorph), a magnet, and a magnetic mount. The piezoelectric element [16][17][18] is to oscillate the cantilever in tapping mode operation; the magnetic mount is to fix the probe; the rest are to enable precise repositioning of the probe which is just newly installed, replacing the previous obsolete one. In normal situation, the cantilever should reveal high Q-factor property in the frequency response so that a slight shift in the driving frequency may induce significant decrease in oscillation amplitude. To prevent this from happening, a function generator based on direct digital synthesized (DDS) technology is incorporated in the system. During the calibration process, the focusing error (FE) signal is monitored to confirm at the middle the linear region of operation. The precision miniature linear stage is to establish the ability to reposition the probe whenever the probe is shifted, and resolution and weight of the stage are 0.01 mm and 0.1 kg, respectively. The above arrangement implies that the repositioning accuracy is improved significantly and the total weight of the AFM platform is still kept light. Overall speaking, the precision miniature linear stage can offer more robust and steadier performance while reducing operational inconvenience. (see Fig. 3(b)). By resorting to water capillary phenomenon, a good environment for liquid scanning has been created. Such a novel design can fit the liquid environment well since we can prevent the probe while scanning the sample from back-and-forth motion between liquid and air media causing excessive disturbance to the path of the laser beam shed on the back of the tip. Another advantageous feature of this design is that we can alleviate the problem with variation of the focal point of the laser beam due to refraction caused by immersing of the probe in liquid medium. The last but not the least, such design also makes the process of calibrating the probe’s light path quite easily in liquid experiment. (a) (b) Figure 3. (a) The view of reduced refraction design, (b) the views of the enlarged kernel parts of the AFM system. Figure 4. The view of the DVD PUH. III. TAPPING MODE TYPE AFM Figure 2. The view of the precision tuning mechanism. In order to scan the sample immersed in the water, one special novel mechanism has been designed (see Fig. 3(a)), so that a cover slip can be inserted between the probe and the CD/DVD PUH lens through the guiding chamfers located at the two inner edges of the above mechanism A. Operation Scheme Tapping mode is a very useful technique. The cantilever of the probe is oscillated vertically by a small piezoelectric element near it is resonance frequency. The amplitude and phase of the cantilever during the scanning process is usually measured by a lock-in amplifier, and the topography image is obtained by monitoring these changes. Because of the short intermittent contact, tapping mode AFM greatly reduces irreversible destructions on sample surfaces, so that it has been widely used for non-destructive imaging of soft and fragile materials such 6550 as polymers and biological samples. The lateral force is also greatly reduced in contrast with the case of contact mode, but the resolution of the imaging is still limited by the tip radius. With the help of the adaptive Q-Control module [19], it is possible to reduce the damping of the dynamic system, i.e. to increase the effective quality factor of the oscillating cantilever and thereby to enlarge the regime of net-attractive interaction forces. Therefore, delicate and highly sensitive surface structures that originally can not be scanned with a standard scanning force microscope can now be characterized with high resolution after applying adaptive Q-Control. As a result, the effective quality (Q) factor of the probe increases momentarily which in turn increases the oscillation amplitude of the probe. This causes a sudden increase in the magnitude of the error signal and the control signal sent to the vertical direction actuator adjusting the position of the sample with respect to the probe tip. The scanning system responds faster to the downward steps, leading to scan speeds faster than the conventional PI controller with or without standard Q control. B. Adaptive Sliding-Mode Controller In order to design a controller which can properly handle piezoelectric nonlinearity and system uncertainty, and meanwhile can gain high robustness and self-tuning property, we propose an adaptive sliding-mode controller for the tapping mode type AFM operation[20] [21]. To start with, a linear second order model can be used to represent the actuator’s nominal dynamics: ɺɺ z + a1 zɺ + a0 z = bu , (3) Nevertheless, we have made some assumptions to simplify the model of the plant. Those assumptions may result in some inaccuracies of the plant model. Thus, two additional disturbance terms need to be added into eq. (3) yielding a model more closer to the real plant, which can be expressed as: ɺɺ e = ɺɺ zd + a1 zɺ + a0 z − bu + wc + wv satisfies || wc (4) + wv ||≦wmax, where wmax is a constant. Then, a sliding surface variable, s, is chosen as: s = eɺ + λ e (5) where λ is positive parameter to be designed. The sliding surface variable is designed such that the system is exponentially stable when the system state constantly lie on the sliding surface. Therefore, the problem is reduced to guaranteeing that the state of the system can reach the sliding surface, or in the case of bounded tracking some region around the surface. The purpose is to force the tracking error to zero or to some very small residual set. Then, taking the time derivative of eq. (5), we have, i.e., sɺ = eɺɺ + λ eɺ = ( ɺɺ zd + a1 zɺ + a0 z − bu + wc + wv ) + λ eɺ (6) Based on eq. (6), the control law is designed as: u AS = bˆ−1 (aˆ1 zɺ + aˆ0 z + ɺɺ zd + wˆ c + λ eɺ + κ s + η sat ( s)) (7) where κ > 0 and η > |wmax|. Moreover, b̂ and ŵc are the estimated values of b and wc, respectively, and sat(.) is the saturation function with boundary layer width ε defined as: 1 s sat ( s ) ≡ ε −1 (1) where z stands for the displacement of the vertical actuator moving the sample, b is the forcing coefficient of the control input u, a1 and a0 represent the damping and the stiffness of the system, respectively. The parameters a1, a0 and b can be well estimated via an off-line identification test. We assume that zd is a desired constant height between tip and sample, and the control goal is to change z to maintain the tip-sample distance at a desired value zd. The tracking error can then be defined as: e ≡ zd − z (2) Substituting eq. (1) into eq. (2), we have : eɺɺ = ɺɺ zd − ( − a1 zɺ − a0 z + bu ) = ɺɺ zd + a1 zɺ + a0 z − bu where wc represents a constant system uncertainty, and wv represents a varying system uncertainty. Note that we here assume that the varying uncertainty term is bounded and s >ε if −ε ≤ s ≤ ε , (8) s < −ε Substituting eq. (7) into eq. (4), we can obtain ɺɺ e = aɶ1 zɺ + aɶ 0 z + wɶ c + w v − bɶ u A S − λ eɺ − κ s − η sa t ( s ) (9) where the estimation errors are defined as: aɶ1 = a1 − aˆ1 , aɶ0 = a0 − aˆ0 bɶ = b − bˆ , wɶ c = wc − wˆ c (10) By applying appropriate gains κ, η, and λ, we can accelerate the convergence and force the error to a small residual error set in a shorter period of time. In the following stability analysis, the adaptation law has to be proposed in order to eliminate the estimation errors as much as possible. Based on adaptive control theory, we define a Lyapunov function candidate V, which is a positive definite function: 1 1 1 1 1 (11) V = s2 + aɶ12τ1−1 + aɶ02τ0−1 + bɶ2τ2−1 + wɶc2τ3−1 2 2 2 2 2 where τ0, τ1, τ2, and τ3, are positive constants. In the 6551 next step, differentiating the Lyapunov function candidate, we obtain: ɶ ɶɺτ −1 + wɶ wɶɺ τ −1 Vɺ = ssɺ + aɶ1aɶɺ1τ 1−1 + aɶ0 aɶɺτ 0 −1 + bb 2 c c 3 (12) Substituting eqs. (6) and (9) into eq. (12), we can derive the following: −1 Vɺ = −s [ηsat (s) − wv ] − κ s2 + aɶ1 (aɺɶ1τ1 + szɺ) ɺ −1 −1 −1 + aɶ0 (aɶɺ0τ 0 + sz) + bɶ(bɶτ 2 − suAS ) + wɶ c (wɶɺ cτ 3 + s) By the latent purpose to make adaptation law as (13) Vɺ ≤ 0 , we design the aɺˆ1 = − aɺɶ1 = τ 1szɺ − τ 1σ 1aˆ1 aˆɺ0 = − aɺɶ0 = τ 0 sz − τ 0σ 0 aˆ0 ɺ ɺ bˆ = −bɶ = −τ 2 su AS − τ 2σ 2bˆ ɺ wˆ c = − wɺɶ c = τ 3 s − τ 3σ 3 wˆ c ɶ ˆσ Vɺ = − s [η sat ( s ) − wv ] − κ s 2 + aɶ1a?1σ 1 + aɶ 0 a0σ 0 + bb 2 + wɶ c wˆ cσ 3 C. Numerical simulation Referring to sweep-sine identification experiment, the parameters of the plant are listed below: a0 = 1.02 × 108 , 2 ≤ −σ 1 aɶ1 + σ 1 aɶ1 a1 2 2 [ aɶ1 − a1 ] Substituting inequalities (16) into (14), we can derive: σ σ 2 2 2 2 Vɺ ≤ − s [η sat ( s ) − wv ] − 1 [ aɶ1 − a1 ] − 0 [ aɶ 0 − a0 ] 2 2 − σ2 ɶ 2 σ 2 2 2 [ b − b ] − 3 [ wɶ c − wc ] 2 2 (17) If 0 < α < min {2κ , σ 0 , σ 1 , σ 2 , σ 3 } is chosen, then we focus on the following two cases: Case 1. From the above discussions in Case 1 and Case 2, we can conclude that for: 1 w2 ε V ≥ V0 = σ 1a12 + σ 0 a02 + σ 2b 2 + σ 3 wc2 + max ,Vɺ ≤ 0 2 4η ɺ which implies that V and V ∈ L∞ . Finally, we can (16) 2 2 (19) further show that the tracking error will converge to a residual set. The plant with uncertainties and the bounded disturbance can be controlled by this adaptive sliding-mode controller stably. According to the control theory, the tracking error will converge to a residual set in order of σ i , i = 0.3 and ε . σ 1aɶ1aˆ1 = σ 1aɶ1 (a1 − aɶ1 ) = −σ 1 aɶ1 + σ 1aɶ1a1 σ1 1 w2 ε ≤ −αV + σ1a12 +σ0a02 +σ2b2 +σ3wc2 + max 2 4η (15) From eq. (19), the range of each term is derived from: 1 1 2 1 2 = −σ 1[ ( aɶ1 − a1 )2 + aɶ1 − a1 ] 2 2 2 s ≤ ε, 1 Vɺ ≤ −s[ηsat(s) − wv ] −αV + σ1a12 +σ0a02 +σ2b2 +σ3wc2 2 s 1 = − s η − wv −αV + σ1a12 +σ0a02 +σ2b2 +σ3wc2 2 ε 1 2η = − s − swv −αV + σ1a12 +σ0a02 +σ2b2 +σ3wc2 ε 2 1 2η ≤ − s + s wmax −αV + σ1a12 +σ0a02 +σ2b2 +σ3wc2 ε 2 2 2 wmax ε wmax ε 1 2η + −αV + σ1a12 +σ0a02 +σ2b2 +σ3wc2 = − s + wmax s − ε 4η 4η 2 (14) Substituting eq. (14) and the inequality η > |wmax| into eq. (13), we can then derive: ≤− Case 2. s >ε , 1 Vɺ ≤ − s [η sat ( s ) − wv ] − αV + σ 1a12 + σ 0 a02 + σ 2b 2 + σ 3 wc2 2 s 1 = − s η − wv − αV + σ 1a12 + σ 0 a02 + σ 2b 2 + σ 3 wc2 2 s 1 = − η s + swv − αV + σ 1a12 + σ 0 a02 + σ 2b 2 + σ 3 wc2 2 1 ≤ −η s + s wmax − αV + σ 1a12 + σ 0 a02 + σ 2b 2 + σ 3 wc2 2 1 = − s (η − wmsx ) − αV + σ 1a12 + σ 0 a02 + σ 2b 2 + σ 3 wc2 2 1 2 2 ≤ −αV + σ 1a1 + σ 0 a0 + σ 2b 2 + σ 3 wc2 (18) 2 a1 = 5.25 × 102 , and b = 2.38 . The parameters of the adaptive sliding-mode controller are listed as follows: λ = 50, κ = 10, η = 1, τ 0 = 10, τ1 = 5, τ 2 = 1, τ 3 = 50 The sliding-mode controller can reduce the tracking error quickly. On the other hand, the adaptive law enables the controller to be equipped with self-correcting capability that may improve performance significantly. As seen in Fig. 5, the controlled z position approaches the desired value, and after 1.4 seconds the error is suppressed within 2 nm. The frequency response can give an overview of the oscillation properties of the system. The resonance curve of the cantilever of the AFM probe will be changed in shape while changing the Q factor. As shown in Fig. 6, o with the phase shift set to 90 , Gset = 50, G0 = 10, and the effective Q factor, Qeff, increased to 300, the resonance peak of the amplitude curve is significantly enhanced. 6552 the liquid and be exempt from a significant force due to the surface tension. This cover slip is 0.12 mm thick, which is thin enough that the laser beam will not be refracted too much. The fine layer of water between the cover slip and the cantilever beam tends to make the laser beam focus on different positions. But the novel design which can clip cover slip can completely solve this problem. Figure 7. The enlarged picture of the core parts of the AFM system. Figure 5. Numerical simulation of z-scanner tracking a standard grating. The reference (blue line) step height is 200 nm. (a) is the simulation result and (b) is the enlargement. Figure 8. Standard grating and depth: 107.5 nm, pitch: 3 µm. B. Scanning results in liquid Figure 6. and G set Amplitude vs. frequency curve with adaptive Q control = 50,G 0 = 10 IV. Experiments A. Hardware Setup The core parts of the experimental setup is illustrated in Fig. 7. As shown in Fig. 7, the CD/DVD PUH is fixed on the frame and relatively above the probe mount, and furthermore the cover slip which are attached to the precision tuning mechanism and cover slip holder. The sample used in the following experiment is a test grating (Calibration grating set TGS1 is intended for z-axis calibration of the scanning probe microscope and nonlinearity measurements, and is formed on the layer of SiO2. The vertical depth is 107.5 nm, and the horizontal pitch is 3 µm, NT-MDT Inc.) with three-dimensional array of small squares on the sample surface as shown in Fig. 8. This calibration grating is a silicon step height standard mounted on 15 mm puck. To ensure the cantilever beam is totally immersed in the liquid, a cover slip is placed over the probe. The liquid will be absorbed between this cover slip and the sample, and hence we can avoid operating the probe at the surface of There are some resonant peaks appearing at different frequencies while the tip is oscillating in liquid. Through the amplitude-distance curve experiment, we can figure out which one is the real resonant frequency. After these pre-works, the scan experiment in liquid can be started. Although the fine layer of water between the cover slip and the cantilever beam will change the focal spot of the laser beam. But the cover slip will be clipped by our novel design during the sample approaching process, and then the thickness of the water layer will not be altered so that the light path calibration can be done before scanning. In other words, the cantilever beam does oscillate at the focal point exactly. Therefore, the signal read from the opto-electric integrated circuit (OEIC) can represent the real bending information of the cantilever, and the corresponding height information can characterize the real topography. The scanning results and the reconstructed 3D topography images in liquid are shown in Figs. 9 and 10. The morphology is clear and identifiable. The scanning ranges are 12 µm × 12 µm and 4.5 µm × 4.5 µm, respectively. The scan speed is 8 seconds per line. The expected result is seen in this figure. Through the above experiments, this proposed system performs satisfactory efficiency while scanning in liquid. Moreover, the implemented mechanism and controllers realize most of the design concept and solve previously encountered problems. In the liquid experiment results, Fig. 10 shows that the original standard rectangular forms become curving. All of the observed discrepancies can be explained as that the 6553 laser beam of the CD/DVD pickup head is refracting the light more or less due to its motion between liquid and air media. But high scanning quality cannot be denied by these discrepancies. In addition, the holistic topography is clear and stable which means this type AFM utilizing DVD pickup head can be employed in the life science field and may lead to significant contribution. [4] [5] [6] [7] [8] [9] [10] Fig. 9. Scanning topography and the virtual 3D topography image in liquid. The total scanning range is 12 µm×12 µm. [11] [12] [13] [14] Fig. 10. Scanning topography and the virtual 3D topography image in liquid. The total scanning range is 4.5 µm×4.5µm. [15] V. Conclusion [16] A novel tapping mode atomic force microscopy (AFM) applying a CD/DVD pickup head (PUH) operating in liquid has been proposed in this paper. 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