© 7990 Oxford University Press
Nucleic Acids Research, Vol. 18, No. 15 4603
Isolation of two different c-ets-2 proto-oncogenes in
Xenopus laevis
C.-M.Wolff, P.Stiegler, M.Baltzinger, D.Meyer, J.Ghysdael1, D.Stehelin1, N.Befort and P.Remy
Laboratoire de Biochimie, Institut de Biologie Moleculaire et Cellulaire du CNRS, 15 rue Rene
Descartes, 67084 Strasbourg Cedex and 1INSERM U186/CNRS URA1160, Institut Pasteur, 1 rue
Calmette, 59019 Lille, France
EMBL accession nos. X51826, X52635
Submitted June 27, 1990
The c-ets proto-oncogene has been identified in two forms, cets-1 and c-ets-2, in several species. By screening a Xenopus
laevis cDNA library prepared from stages l/II oocyte mRNAs
with a chicken c-ets-2 cDNA probe, two different c-ets-2 cDNAs
have been identified. These cDNAs of 1.1 kb and 1.3 kb, named
Xl-ets-2a and Xl-ets-2b respectively, share 91.3% similarity.
They most likely arise from two different Xl-ets-2 genes rather
than from different alleles. When compared with c-ets-2 genes
from humans, mouse and chicken, both Xenopus genes show
similarities ranging from 66.5% to 72.7%.
The two Xenopus c-ets-2 cDNA partial sequences encode
polypeptides of 352 amino acids which share 95.2% identical
residues; if conservative amino acid changes are considered, the
1
GAA TTC TCC TTG GAG
C
S
F
E
L
E
E
F
s L Q
91
Xl-ets-2b GCA CCT GAC TTC GTT
T
Xl-ets-2a
T
P
A
154
V
F
D
P
A
V
F
D
181
Xl-ets-2b CAT TCT AAC CGG GAC
XI-ets-2a
A
S
H
184
R
D
N
S
H
D
N
Q
271
Xl-ets-2b AAT TAC CCT AAA AAT
Xl-ets-2a
Y
N
214
P
K
N
N
Y
P
N
K
361
Xl-ets-2b CCC AGC TCT TGC CTT
Xl-ets-2a
P
S
244
S
L
C
P
S
S C
L
451
Xl-ets-2b CTA AAT TCT GGC AAA
Xl-ets-2a
G
C
274
N
L
S
G
K
L
N
S
G
K
541
Xl-ets-2b AGC CAA TCT TCT CTT
G
Xl-ets-2a
Q
S
304
S
S
L
5
Q
s s L
Xl-ets-2b
Xl-ets-2a
124
sequence similarity is 97.7%. The Xl-ets-2 polypeptides are
highly conserved with 68 to 70% of their amino acids identical
to those of the human, mouse, and chicken ets-2 proteins. The
most conserved region maps in the C-terminus of the protein and
contains both the DNA binding site and the nuclear translocation
signal.
Northern blot analysis revealed that the Xl-ets-2 genes are
transcribed during oogenesis and embryonic development into
a major 3.2 kb RNA and a minor 1.7 kb transcript. The same
transcripts were also detected in a variety of adult tissues (brain,
eye, liver, stomach, spleen, heart, kidney, muscle, ovary and
testis).
90
AAC GTG AAT TTT CAG AAG TTT CTC ATG AAT GGA CAC GAG CTA TGC AGC CTC GGG AAG GAG AGG TTT TTG GCT CTG
A
G
A
L
V
N
N
L
F
R
E
L
K
F
K
N
G
E
H
L
G
S
C
M
F
L
Q
V
N
N
C
F
L
A
L
F
R
E
K
F
K
N
G
E
H
L
G
S
M
L
L
Q
180
GGG GAC ATA CTT TGG GAA CAT CTG GAA GAA ATG ATG AAA GAG TAT CAA GAA AAG GCC CAG GAG CCA TAT ATT GAC
C
G
C
A
G
A C
I
I
D
Y
L
W
D
G
Y
E
P
E
H
E
E
M
K
M
E
K A
E
L
Q
Q
I
I
D
Y
L
W
D
G
P
E
H
E
E M
K
M
E
K A
H
E
E
L
Q
Q
270
TCA CTA AAC CAG TGG ATG AAT GCA GAT TCC TTA AAT TTC ACT GCT GAT CCG TTG CAG TGT GGG GCA CAA GTA CAC
C A
A
T
T
C A
V
H
S
P
N
Q
A
G
C
A
N
M
S
L
L
W
F
N
L Q
D
D
A
T
Q
V
H
A
S
I
W
P
N
Q
A
G
C
N
M
S
L
F
N
L Q
T
D
D
H
T
360
GGA ATG TAT AAT GAT ATG TGC TCA GTG CCC ACT GGT CAG ACT CTC CTC AAC CCC AAA CAA GAG TTC CAA CAA TAC
T
C A
A
T
P
Y
E
K
S
G
M
D
V
Y
N
N
Q
F
C
M
T
P
G
L
L
T
Q
Q
Q
Y
S
P
G
P
M
V
Q
F
E
C
K
M
T
D
G
L
F
N
Q
Q
L
T
Q
Q
450
AAA TCC AGA GCA GTA AAC TAC CCG CCC GCC AGC CAG GAC TTT GCA AGG AGC CAT ATG AAC GTG TTG CTC AAC TCT
T
T A
A
G
T CA C
S
L
N
V
H M
K
V
S
R
p A
A
S
R
P
L
N
Y
N
A
5
F
D
Q
S
L
N
N M
K
V
S
R
p A
S
R
S
L
A
N
Y
N
S
A
D
F
A
Q
540
TTG AGA GAC TAT GAC TCA GGA GAC AGC GGC ACA GAA AGC TTC GAG AGC ACC GAA TCA CTA CTG CAT TCT TGG ACG
T
G
A
T
G
T
T
R
Y
T
S
W
S
S
L
T
D
D
H
L
L
G
S
E
S
D
G
T
E
F
S
E
T
S
W
S
S
S
L
R
Y
T
D
D
L
L
G
S
E
D
G
T
E
F
E
S
Q
630
GTC GAT ATG CAA CGA GTT CCT TCA TAT GAC AGC TTT GAG GAA GAT GGC AAT CAA ACG TTG TGT CTA AAT AAG CAA
C
C
C
A
G G
C
G
GC
T
V
N
K
P
V
R
D
G
N
S
M
Q
L
C
L
T
Y
D
S
F
D
Q
Q
E
E
P
N
K
P
R
V
D
M
C
L
A
V
Y
D
G
S
G
S
L
Q
F
D
E
E
Q
* To whom correspondence should be addressed
4604 Nucleic Acids Research, Vol. 18, No. 15
Xl-ets-2b
Xl-ets-2a
334
Xl-ets-2b
Xl-ets-2a
364
Xl-ets-2b
Xl-ets-2a
394
Xl-ets-2b
Xl-ets-2a
424
Xl-ets-2b
Xl-ets-2a
454
Xl-ets-2b
Xl-ets-2a
Xl-ets-2b
631
720
CCA ATG TCC TTC AAA GAT TAT ATC CAG GAC AGG TGT GAG CCA GCA GAA CTA GGC AAA CCT GTT ATT CCC GCA TCT ATA CTT GCT GGC TTC
T
T
C
A
A
G G
A
P
M
S
F
K
D
Y
I
Q
D
R
C
E
P
A
E
L
G
K
P
V
I
P
A
S
I
L
A
G
F
P
M
S
F
K
D
Y
I
Q
D
R
C
E
P
A
E
L
G
K
P
V
I
P
A
S
I
L
A
G
F
721
810
ACA GGC AGT GGA CCG ATC CAA TTG TGG CAG TTC TTG TTG GAA CTT TTA ACG GAT AAG TCA TGT CAG TCC TTT ATC AGC TGG ACT GGG GAT
A
A
GC
A
G
C
T
G
S
G
P
I
Q
L
W
Q
F
L
L
E
L
L
T
D
K
S
C
Q
S
F
I
S
W
T
G
D
T
G
S
G
P
I
Q
L
W
Q
F
L
L
E
L
L
T
D
K
S
C
Q
S
F
I
S
W
T
G
D
811
900
GGA TGG GAG TTC AAG CTG GCT GAC CCA GAT GAG GTG GCC CGG CGG TGG GGT AAA AGG AAA AAC AAA CCT AAA ATG AAC TAC GAG AAG CTA
A
C
C
T
G
W
E
F
K
L
A
D
P
D
E
V
A
R
R
W
G
K
R
K
N
K
P
K
M
N
Y
E
K
L
G
W
E
F
K
L
T
D
P
D
E
V
A
R
R
W
G
K
R
K
N
K
P
K
M
N
Y
E
K
L
901
990
AGT CGA GGA CTA AGG TAT TAT TAT GAC AAG AAC ATC ATC CAC AAG ACA TCA GGG AAG AGA TAC GTG TAC CGA TTT GTT TGT GAC CTT CAC
C
C
T
G G
C
C
S
R
G
L
R
Y
Y
Y
D
K
N
I
I
H
K
T
S
G
K
R
Y
V
Y
R
F
V
C
D
L
H
S
R
G
L
R
Y
Y
Y
D
K
N
I
I
H
K
T
S
G
K
R
Y
V
Y
R
F
V
C
D
L
H
991
1087
AAC TTA TTG GGC TAC ACG CCG GAT GAA TTG CAC GCA ATG CTT GGA GTA CAG CCC GAC ACA GAC GAA TGAGCTGCCGGAGTGTGAACTAAATCATTGG
C
G
T
A
C C
T
C
G
N
L
L
G
Y
T
P
D
E
L
H
A
M
L
G
V
Q
P
D
T
D
E
*
N
L
L
G
Y
T
P
D
E
L
H
A
M
L
G
V
Q
P
D
T
D
E
*
1088
1206
TTTGAAGAGCACATTATTGTACTGTGAAAAGGATCGTTTA
C
C
A 3'-end
1285
TGCTCTGTTTTCTTAGATCGGAGTAAAGATCTGATGGATGGCGCTTATTGTTATACAGTTGCTCACTTGAAAAAAAAAA