MASTER CLASS
ATP HYGIENE MONITORING & RAPID TESTS FOR
COLIFORM/E.COLI/TOTAL COUNT
9TH FOOD SAFETY AND & QUALITY SUMMIT
NEW DELHI 2&3 DECEMBER 2014
ATP Hygiene Monitoring
What is it?
•A test method to verify good cleaning wherever you want it,
whenever you want it
•Portable, easy and ready for use
•Tests can be done at line, giving instant feedback on status of
cleanliness
Ideal for industries with a need for rapid cleaning control and
who want to improve their hygiene and quality standards
Key advantage is speed and ease-of-use
PURPOSE OF CLEANING
• Primary purpose is to remove organic soiling i.e.
– Environmental Cleaning (Stage 1)
– Also reduced microbial bioburden
– Ideal test is a direct, instant test for organic residue e.g. ATP
• Only after good cleaning can disinfectants can be applied with
confidence
– Disinfection Cleaning ( Stage 2)
• Hygiene monitoring is an assessment of the cleaning regime
over time i.e. supervise and inspect
– Are standards being maintained? Can we improve it?
PROVEN TECHNOLOGY
• ATP tests have been around since late 80’s and are approved
as rapid cleaning verification test
• British Retail Consortium have developed a global standard
for food safety and quality, involving ATP testing. Edition 6,
page 40 advises the use of ATP for cleaning verification.
• Compliant with HACCP
• Value for money
ATP HYGIENE MONITORING SYSTEM
Two part system composed of
1. Hardware = Detection instrument
• supported by data analysis software
2. Swab device for sample collection and testing
Designed for:
Simplicity ( easy of use)
Convenience
Robustness
Affordability
Sustainability
Multi-functional
COMPARISON WITH OTHER METHODS
What is clean?
Visual
Microbiology
ATP
Fast?
++
---
++
Sensitive?
---
++
++
Detection of product residues?
+
--
++
Simple?
++
--
++
WHAT IS ATP?
ATP stands for Adenosine-Tri-Phosphate, which is the energy source of all living
organisms and food.
If ATP is detected on a surface, it means it has not been cleaned properly.
Sources of ATP:
Food
Microorganisms
People
MEASUREMENT PRINCIPLE
Detection of ATP by bioluminescence reaction
ATP reacts with luciferine/luciferase enzyme complex to generate a light
reaction, which is measured in a sensitive and dedicated luminometer
The ATP test is the most common used rapid test in the world to determine
cleaning efficiency.
ATP VS CLEANING RESULT
high
ATP (expressed in RLU)
low
Cleaning result
excellent
very poor
The more food residues or microorganisms, the more ATP is
detected, the higher the light output.
TWO FORMATS
Ultrasnap & Supersnap
• For surface hygiene monitoring
• For flat as well as for irregular surfaces
• Immediate result
Aquasnap
• For water samples (like rinse water after Cleaning in Place, CIP)
• Dip Aquasnap into the water
• Immediate result
PROCEDURE
2
Swab
3
Snap
4
Squeeze
Read
SIMPLE INTERPRETATION
Interpretation is done by pass/fail limits, which are
already pre-set in the instrument.
For the most common food contact surface (stainless
steel), these pass/fail limits are:
Pass: <10 RLU
(√)
Caution: 11-30 RLU
(!)
Fail: >30 RLU
(X)
BENEFITS OF ATP SYSTEM
• Simple and easy to use by anyone , anywhere, any time
• Providing instant objective quantifiable data
– Permits immediate corrective action
• Identify hot spots and potential risk areas
• Optimises cleaning processes
• Demonstrates and reinforces Best Practice
• Provide real data for Quality monitoring ,
– evidence of due diligence,
– value for money
• Supports training and awareness
• Enables team building through more effective direct communication
• Restores pride, ownership and commitment of cleaning staff
• Encourages behaviour change
Clean it …… Prove It!
WELL KNOWN HYGIENA USERS
MICROSNAP
RAPID DETECTION OF
COLIFORMS/E.COLI & TOTAL COUNT
MICROSNAP RANGE
These are applications that require sample preparation and
handling and therefore cannot be done at line. But
nevertheless very simple and reliable.
• Microsnap Coliform/E.coli – a very rapid and low cost
screening test for Coliforms and E.coli. Total test time 6-8
hours.
• MicroSnap Total – a rapid low cost screening test for total
viable counts. Total test time 7 hours.
MICROSNAP COLIFORM/E.COLI
SPECIFIC DETECTION
Sensitive luminometer
Microbial detection
Specific enzyme detection
AOAC approved
SAMPLE TYPES
•
•
•
•
Surface swabs
Raw material and finished product
Solids , liquids and filterable products
Food & beverage; dairy; cosmetics & pharma
and water
TEST PROCEDURE
After incubation,
transfer 100µl from
the enrichment
device (A) and
transfer to the
detection device (B).
100µl
Activate detection
device (B) with a
snap and a
squeeze.
Read RLU in luminometer
Interpret RLU results
Incubate for
10 minutes at
37°C
INCUBATION TIME
Incubation Time (37C)
(h)
Lowest Inoculum Detected
with 95% certainty
1
<500000
2
<100000
3
<10000
4
<1000
5
<100
6
<10
7
<5
8
1
VIDEO
MICROSNAP TOTAL
• Sensitive luminometer
• Two-step procedure
• 7 Hours incubation (Step 1), followed by
immediate measurement (Step 2)
• AOAC approval pending
SAMPLE TYPES
•
•
•
•
Surface swabs
Raw material and finished product
Solid and liquid samples
Food & beverage; dairy; cosmetics & pharma
and water
TEST PROCEDURE
100µl
After incubation,
transfer 100µl from
the enrichment
device (A) and
transfer to the
detection device (B).
Activate detection
device (B) with a
snap and a
squeeze.
Read result in
luminometer
Interpret RLU results
INTERPRETATION OF RESULTS
Correlation between CFU/ mL and average RLU After 7 hour
Incubation at 30°C
CFU per ml/100cm2
< 100
< 200
< 300
< 500
< 1,000
< 10,000
< 100,000
VIDEO
EnSURE RLU
< 10
< 20
< 30
< 50
< 100
< 1,000
< 9,999
THANK YOU