Electronic Supplementary Material (ESI) for Lab on a Chip
This journal is © The Royal Society of Chemistry 2012
Electronic Supplementary Information (ESI) for Lab on a Chip. This Journal is © The Royal Society of Chemistry. 2011 Microreactor with integrated temperature control for the synthesis of
CdSe nanocrystals
Sara Gómez-de Pedro, Cynthia S. Martínez-Cisneros, Mar Puyol and Julián Alonso*
*Corresponding author: Fax: 34 9358 12477 Tel: 34 9358 12533; E-mail: [email protected]. Sensors &
Biosensors Group, Department of Chemistry, Autonomous University of Barcelona, Edifici Cn, 08193 Bellaterra,
Catalonia, Spain.
Electronic Supplementary Information (ESI)
Table of contents
Table of contents ......................................................................................................................... 1
Temperature characterization of the microsystem ....................................................................... 2
Supplementary figures
Fig S3: Correlation peaks with temperature .......................................................................... 3
Fig S4: Stokes shifts.............................................................................................................. 4
1
Electronic Supplementary Material (ESI) for Lab on a Chip
This journal is © The Royal Society of Chemistry 2012
Electronic Supplementary Information (ESI) for Lab on a Chip. This Journal is © The Royal Society of Chemistry. 2011 Temperature characterization of the microsystem
The microsystem was thermally evaluated using an EMC scanner with an IR Probe
(RS321EH, Detectus AB) in order to estimate the temperature distribution.
The isothermal maps of the thermal and fluidic platforms in the xy-axis were recorded,
obtaining a uniform radial distribution in all cases. Since the maps were highly similar, only
one of them is presented in figure S1, corresponding to the distribution of temperature on
the top of the microfluidic platform. The presented isothermal map was obtained by
coupling mechanically the microfluidic platform over the heating module, as in the synthesis
of nanocrystals, and maintaining temperature in the sensor zone at 270 ºC.
Fig. S.1 (A) Isothermal map obtained for the microfluidic platform coupled to the thermal one; (B) image of the
corresponding microfluidic module. The two black circles represents where the microfluidic channels are placed.
MICROFLUIDICS
To determine the thermal gradient in the z-axis, punctual measurements on the area of the
PT100 sensor were performed on both sides of the microsystem (thermal and microfluidic).
Temperature was found to be 270ºC and 255ºC on the bottom of the thermal and on the
top of the microfluidic platforms, respectively.
Considering the number of layers used for its fabrication and the placement regarding both,
the microchannel and the resistor in the microsystem (Figure S.2), a temperature of
268.2ºC can be assumed in the microfluidic channel.
Embedded
Channel
THERMAL
Embedded
Resistor
PT100
Fig. S.2 Schematic diagram of the temperature analysis developed to estimate temperature in the microfluidic
channel.
2
Electronic Supplementary Material (ESI) for Lab on a Chip
This journal is © The Royal Society of Chemistry 2012
Electronic Supplementary Information (ESI) for Lab on a Chip. This Journal is © The Royal Society of Chemistry. 2011 Supplementary figures
Fig S3: Correlation peaks with temperature
300
280
Temperature / ºC
260
240
220
200
180
Em
160
480
500
520
540
560
i ss
io n
wa
ve
le n
460
440
580
gth
420
600
/n
400
m
Ex c
i ta ti
on
e le
wav
480
/n
n g th
m
Temperature / ºC
300
280
260
240
220
200
180
i on
iss
Em
wa
160
480
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520
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580
le
ve
ng
/
th
420
600
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480
460
440
nm
on
itati
Exc
nm
th /
l eng
e
v
wa
300
Temperature / ºC
280
260
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180
ion
iss
Em
le
ve
wa
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480
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520
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560
580
th
ng
600
460
440
420
400
/n
on w
itati
Exc
len
a ve
g th
480
/ nm
m
Fig. S.3 Correlation between fluorescence emission peaks, excitation wavelengths and temperatures during the
synthesis of nanocrystals with a Cd/Se molar ration of 1:10, 1:1 and 1:2 (from top to bottom).
3
Electronic Supplementary Material (ESI) for Lab on a Chip
This journal is © The Royal Society of Chemistry 2012
Electronic Supplementary Information (ESI) for Lab on a Chip. This Journal is © The Royal Society of Chemistry. 2011 Fig S4: Stokes shifts
140
∆ Stokes
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90
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Temperature / ºC
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∆ Stokes
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Temperature / ºC
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∆ Stokes
120
110
100
90
80
160
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220
240
300
Temperature / ºC
Fig. S.4 Stokes shifts for the synthesized quantum dots at different temperatures and molar relationship of reagents
(from top to bottom 1:10, 1:1 and 1:2). The mean value, the standard deviation and the error bars are represented.
4