Exogenous DNA Loading into Extracellular Vesicles via Electroporation is Size-Dependent
and Enables Limited Gene Delivery
Tek N. Lamichhane1, Rahul S. Raiker1 and Steven M. Jay1,2,3*
1
Fischell Department of Bioengineering
2
Program in Oncology, Marlene and Stewart Greenebaum Cancer Center
3
Program in Molecular and Cell Biology
University of Maryland
College Park, MD 20742 USA
Supporting Information
1
Figure S1: EV size before and after electroporation. EV size increases slightly following
electroporation from an average diameter of 101 nm to 135 nm.
2
Figure S2: Evaluation of protein expression following gene transfer. Immunoblotting for green
fluorescent protein (GFP) expression was carried out on HEK293T cell lysates following:
incubation in control media (Control); transfection with GFP plasmid via Lipofectamine 3000
using standard methods (Lipofectamine); and transfection with EVs loaded with GFP-plasmid
via electroporation under the same conditions as reported in figure 7 (EVs). GAPDH was used as
a loading control.
3