On the heat stability of amyloid-based biological activity: insights from thermal
degradation of insulin fibrils.
(Thermal degradation of insulin fibrils)
Weronika Surmacz-Chwedoruk, Iwona Malka, Łukasz Bożycki, Hanna Nieznańska,
Wojciech Dzwolak
Supporting Information
Repeated TGA/DSC scans of human insulin amyloid samples: demonstration of the high
degree of reproducibility of the data in Figure 2.
m/z
100
exo
428 ± 2.0
Mass [%]
Tend = 635 ± 0.5 -1
60
-2
503 ± 3.0
40
100
200
300
400
500
Temperature [°C]
600
700
1
(H2O; NH3) 17
≈
(HCNO) 43
6
Ion Current * 10-12 [A]
319 ± 1.5
(H2O) 18
(CH4; NH3) 16
-3
20
(CO2) 44
2
Heat flow [µW/mg]
0
80
Tonset = 285 ± 0.7
Ion Current * 10-11 [A]
3
≈
(H2S) 34
(H2S) 33
4
(NO) 30
(HCN) 27
(HCN) 26
2
(CH4) 15
(C; CO2) 12
100
200
300
400
500
Temperature [° C]
600
700
Figure S1.
Figure S1 shows several overlapping TGA and DSC traces (left panel: black and red lines,
respectively) and corresponding MS profiles of released gases (right panel) for repeated
experiments depicted in Figure 2 of the main article.