DRUGS OF ABUSE
DRI BARBITURATE SERUM TOX (SBRX)
DRI BARBITURATE SERUM TOX (SBRX) FOR THE UniCel DxC SYNCHRON CLINICAL SYSTEMS
The DRI Barbiturate Serum Tox Assay is intended for the qualitative and semi-quantitative determination of
barbiturates in human serum or plasma, using a 1µg/mL cutoff calibrator.
Various barbiturates, such as secobarbital (short-acting) and phenobarbital (long-acting), are subject to
abuse. Barbiturate abuse can lead to respiratory depression or coma in severe cases. Therapeutic serum
concentration and toxic level for each barbiturate are different. In addition, patients who have used
barbiturates habitually, particularly those who are addicted to such agents, may tolerate far larger dosages
than persons who are not habitual users. Because of the wide variations in individual tolerance and variation
in toxic levels associated with each barbiturate, serum tox immunoassays are useful primarily to establish
the presence of the agent. An alternate chemical method should be used to determine the identity and exact
concentration of the specific barbiturate. Being able to determine the type of barbiturate ingested will facilitate
an effective course of treatment for barbiturate intoxication.1 Although the detection of barbiturates in urine
can be used as an indication of barbiturates usage, the serum and plasma and barbiturate assay can be
extremely important in emergency situations where a urine sample may be difficult to obtain.
Many conventional techniques, such as TLC, GC, GLC and HPLC, are available for testing abused drugs
in biological fluids. Immunoassays, based on the specific recognition of antigen (abused drugs) by the
corresponding antibody, are available for high volume screening applications. DRI Barbiturate Serum Tox
Assay is a homogeneous enzyme immunoassay using ready-to-use, liquid reagents.2 The assay uses specific
antibodies which can detect most barbiturates in serum.
FEATURES OF THE DRI BARBITURATE SERUM TOX (SBRX)
› Choice of qualitative or semi-quantitative measuring method
› Serum and plasma as acceptable sample types
› Ready-to-use, liquid reagents
› On-board reagent stability of 60 days
› Calibration stability of 14 days
› Total imprecision of <11% CV
› Cutoff of 1000 ng/mL
LAB FORWARD
DRI BARBITURATE SERUM TOX (SBRX)
PERFORMANCE CHARACTERISTICS
Analytical Sensitivity
DxC
The following tables present the sensitivity results generated from the UniCel DxC systems:
0.063 µg/mL
Typical Precision
A properly operating UniCel DxC system should exhibit precision values less than or comparable to the following:
Total Run Precision (Qualitative)
Within-Run Precision (Qualitative)
DxC
DxC
Mean
(mA/min)
SD
%CV
Mean
(mA/min)
SD
%CV
Serum Tox Cal 1
(0.5 µg/mL)
159.2
1.01
0.63
Serum Tox Cal 1
(0.5 µg/mL)
159.2
1.21
0.76
Cutoff Calibrator
(1.0 µg/mL)
171.8
1.00
1.58
Cutoff Calibrator
(1.0 µg/mL)
171.8
1.18
0.69
Serum Tox Cal 3
(3.0 µg/mL)
199.2
1.12
0.56
Serum Tox Cal 3
(3.0 µg/mL)
199.2
1.72
0.86
Within-Run Precision (Semi-Quantitative)
Total Precision (Semi-Quantitative)
DxC
DxC
Mean
(µg/mL)
SD
%CV
Mean
(µg/mL)
SD
%CV
Serum Tox Cal 1
(0.5 µg/mL)
0.498
0.029
5.88
Serum Tox Cal 1
(0.5 µg/mL)
0.498
0.045
9.03
Cutoff Calibrator
(1.0 µg/mL)
0.997
0.055
5.53
Cutoff Calibrator
(1.0 µg/mL)
0.997
0.088
8.84
Serum Tox Cal 3
(3.0 µg/mL)
2.99
0.123
4.12
Serum Tox Cal 3
(3.0 µg/mL)
2.99
0.285
9.52
BECKMAN COULTER
REFERENCE NUMBER ITEM
A45320DRI Barbiturate Serum Tox Reagent Kit (25 mL / 8 mL)
A45326
DRI Serum Tox Negative Calibrator (10 mL)
A45327
DRI Serum Tox Calibrator 1
A45328
DRI Serum Tox Calibrator 2
A45331
DRI Serum Tox Calibrator 3
A45332
DRI Serum Tox Calibrator 4
442835
User Defined Reagent Cartridges (pkg of 12)*
A45333
Instructions for Use**
QUALITATIVE
SEMI-QUANTITATIVE
Yes
No
Yes
No
Yes
Yes
Yes
Yes
Yes
Yes
*Sold separately
**Ordered separately
1.Hadden J. et al. Acute barbiturate intoxication. 1969;209:893-900.
2.Rubenstein KE, Schneidter RS, EF Ullman. Homogeneous enzyme immunoassay: a new immunochemical technique.
Biochem Biophys Res Commun 1972;47:846-851.
DRI® is a registered trademark of Thermo Fisher Scientific Corporation.
Distributed by: Beckman Coulter, Inc. for UniCel DxC Synchron Clinical Systems
Manufactured by:
Thermo Fisher Scientific
Diagnostic Division, Seradyn Products
7998 Georgetown Road, Suite 100
Indianapolis, IN 46268
U.S.A.
Beckman Coulter, the stylized logo, UniCel and Synchron are trademarks of Beckman Coulter Inc.
and are registered in the USPTO.
For Beckman Coulter’s worldwide office locations and phone numbers, please visit
www.beckmancoulter.com/contact
DS-11627B B2013-14560
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