AR09297PU-N
OriGene Technologies Inc.
9620 Medical Center Drive, Ste 200
Rockville, MD 20850
UNITED STATES
Phone: +1-888-267-4436
Fax: +1-301-340-8606
[email protected]
OriGene EU
Acris Antibodies GmbH
Schillerstr. 5
32052 Herford
GERMANY
Phone: +49-5221-34606-0
Fax: +49-5221-34606-11
[email protected]
Human Galectin-3 (1-250, His-tag) - Purified
Alternate names:
35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBP, Galactose-specific
lectin 3, IgE-binding protein, L-31, L-34 galactoside-binding lectin, Laminin-binding protein,
Lectin L-29, Lgals3, Mac-2
Catalog No.:
AR09297PU-N
Quantity:
0.1 mg
Concentration:
0.5 mg/ml (determined by Bradford assay)
Background:
Galectin-3 is a member of the family of animal lectins, which selectively binds betagalactoside residues. This protein is secreted from cells by ectocytosis, which is
independent of the classical secretory pathway through the endoplasmic reticulum/Golgi
network. Galectin-3 has been associated with the inhibition of apoptosis and the
progression of cancer. It is normally distributed in epithelia of many organs, in various
inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The
expression of this lectin is up-regulated during inflammation, cell proliferation, cell
differentiation and through trans-activation by viral proteins.
Uniprot ID:
P17931
NCBI:
NP_002297
GeneID:
3958
Species:
Human
Source:
E. coli
Format:
State: Liquid purified protein
Purity: >95% pure by SDS – PAGE
Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 1 mM DTT, 0.1 M
NaCl
Description:
Recombinant Galectin-3 protein, fused to His-tag, was expressed in E.coli and purified by
using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH
PGAYPGQAPP
PLPGGVVPRM
PFESGKPFKI
SSGLVPRGSH
GAYPGAPGAY
LITILGTVKP
QVLVEPDHFK
MADNFSLHDA
PGAPAPGVYP
NANRIALDFQ
VAVNDAHLLQ
LSGSGNPNPQ
GPPSGPGAYP
RGNDVAFHFN
YNHRVKKLNE
GWPGAWGNQP
SSGQPSATGA
PRFNENNRRV
ISKLGISGDI
AGAGGYPGAS YPGAYPGQAP
YPATGPYGAP AGPLIVPYNL
IVCNTKLDNN WGREERQSVF
DLTSASYTMI
Specific Activity: The ED50 for this effect is less or equal to 15 µg/ml. Measured by its
ability to agglutinate Human red blood cells.
Molecular weight: 28.3 kDa (270 aa)
Storage:
Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
For research and in vitro use only. Not for diagnostic or therapeutic work.
Material Safety Datasheets are available at www.acris-antibodies.com or on request.
Acris Antibodies is now part of the OriGene family. Learn more at www.origene.com
MS/20170105
1/2
AR09297PU-N: Human Galectin-3 (1-250, His-tag) - Purified
General Readings:
1. Barondes SH., et al. (1994) J Biol Chem. 269(33):20807-10.
2. Kadrofske MM., et al. (1998) Arch Biochem Biophys. 349(1):7-20.
Pictures:
Recombinant human Galectin-3, (1-250
aa), His-tagged: 15% SDS-PAGE (3 µg)
For research and in vitro use only. Not for diagnostic or therapeutic work.
Material Safety Datasheets are available at www.acris-antibodies.com or on request.
Acris Antibodies is now part of the OriGene family. Learn more at www.origene.com
Powered by TCPDF (www.tcpdf.org)
MS/20170105
2/2