Supplementary Figure 1: Correlation between map organization and cortical activation.
Graph showing the correlation between map organization and cortical activation across different conditions
and treatments. Map organization (scatter) is plotted on the Y axis and the cortical activation (ΔR/R) on the
X axis. Each point represents one animal from the experimental groups summarized in the graph legend.
Methods: To quantify visual map organization we measured scatter within maps using custom Matlab
software according to methods previously described (Smith and Trachtenberg, 2007). Briefly, in the
retinotopic representation each pixel corresponds to a value (Cortical Organization Map or COM value)
representing the cortical representation of the bar in the visual field. If the map is ordered, points in close
proximity in the visual field will be close in the cortical representation and will have a similar COM value.
Scatter was calculated for the top 50% activated pixels as the average difference between the COM value
of each pixel and the 24 surrounding pixels; the lower the scatter the more organized the cortical
representation of visual space.
Supplementary Figure 2: Effects of visual experience on the motility of individual spine classes.
The motility index of individual spine classes (mushroom, stubby, thin and filopodia) across different
conditions. No significant differences were observed.
Supplementary Figure 3: Time course of changes in visual responses following brief exposure to
light after dark rearing.
A. Representative images of the cortical intrinsic signal in response to light in individual mice from the
different groups imaged. Red hues indicate strong activation, as per dR/R key at right. Following brief
exposure to light, visually evoked activation is increased as compared to dark reared animals. Scale bar=
0.5 mm.
B. Quantification of the visually evoked optical signal across animals with different visual experience. The
strength of the signal (normalized change in reflectance, dR/R) is low in dark reared animals and increases
in animals exposed to light for 2-4 hours. This effect disappears after longer visual exposures and cortical
activation returns to dark rearing levels after two days of light exposure.
Supplementary Table 1
Motility
(µm/min)
0.020
0.021
0.024
0.025
0.024
0.019
mushroom
(%)
52.1
71.4
66.7
64.3
60.9
60.5
stubby
(%)
34.4
23.8
22.2
28.6
18.8
20.9
thin
(%)
13.5
4.8
0.0
0.0
13.0
18.6
filopodia
(%)
0.0
0.0
11.1
7.1
7.2
0.0
immaturity
index
0.00315
0.00104
0.00301
0.00192
0.00612
0.00432
density
(protrusions/µm)
0.62
0.66
0.57
0.45
0.45
0.53
DR1
DR2
DR3
DR4
DR5
DR6
DR7
0.024
0.023
0.025
0.047
0.031
0.030
0.026
58.2
76.7
54.2
57.9
56.1
50.0
40.9
17.6
0.0
18.8
10.5
17.5
10.0
31.8
19.8
20.0
18.8
21.1
26.3
25.0
18.2
4.4
3.3
8.3
10.5
0.0
15.0
9.1
0.00780
0.00686
0.00927
0.02148
0.01096
0.01998
0.00959
0.51
0.57
0.66
0.41
0.63
0.47
0.50
DR
DR
DR
DR
DR
DR
DR
DR2L1
DR2L2
DR2L3
DR2L4
DR2L5
DR2L6
0.027
0.036
0.029
0.031
0.021
0.034
48.1
61.8
37.3
56.8
38.9
40.0
33.3
2.9
37.3
24.3
33.3
45.0
7.4
20.6
25.5
8.1
25.0
10.0
11.1
14.7
0.0
10.8
2.8
10.0
0.00610
0.01940
0.00995
0.00716
0.00797
0.00806
0.38
0.40
0.43
0.47
0.67
0.28
DR/2dL
DR/2dL
DR/2dL
DR/2dL
DR/2dL
DR/2dL
DR7L1
DR7L2
DR7L3
DR7L4
DR7L5
DR7L6
0.033
0.019
0.023
0.017
0.027
0.028
55.6
73.3
70.0
64.3
46.9
53.8
11.1
13.3
5.0
28.6
28.1
15.4
18.5
10.0
15.0
0.0
25.0
23.1
14.8
3.3
10.0
7.1
0.0
7.7
0.01626
0.00294
0.00755
0.00133
0.00903
0.01255
0.57
0.52
0.66
0.57
0.36
0.38
DR/7dL
DR/7dL
DR/7dL
DR/7dL
DR/7dL
DR/7dL
DR2h1
DR2h2
DR2h3
DR2h4
DR2h5
DR2h6
DR2h7
0.033
0.026
0.033
0.057
0.033
0.034
0.038
58.3
59.5
44.6
44.4
46.6
34.5
33.3
4.2
5.4
25.0
22.2
19.2
13.8
19.0
25.0
21.6
26.8
17.8
21.9
44.8
42.9
12.5
13.5
3.6
15.6
12.3
6.9
4.8
0.01982
0.01428
0.01427
0.02843
0.01725
0.03640
0.03411
0.56
0.54
0.48
0.27
0.51
0.76
0.80
DR/2hL
DR/2hL
DR/2hL
DR/2hL
DR/2hL
DR/2hL
DR/2hL
LCPP1
LCPP2
LCPP3
LCPP4
LCPP5
LCPP6
0.029
0.027
0.027
0.037
0.032
0.028
59.1
36.8
61.5
56.1
29.9
54.2
18.2
36.8
15.4
15.8
34.5
33.3
22.7
26.5
23.1
21.1
33.3
12.5
0.0
0.0
0.0
7.0
2.3
0.0
0.00855
0.00974
0.00824
0.01432
0.01792
0.00395
0.45
0.51
0.57
0.44
0.46
0.35
DR/2hL+CPP
DR/2hL+CPP
DR/2hL+CPP
DR/2hL+CPP
DR/2hL+CPP
DR/2hL+CPP
DRCPP1
0.033
38.1
42.9
14.3
4.8
0.00773
0.43
DR+CPP
CTRL1
CTRL2
CTRL3
CTRL4
CTRL5
CTRL6
condition
control
control
control
control
control
control
DRCPP2
DRCPP3
0.030
0.026
66.7
44.7
6.7
38.3
23.3
17.0
3.3
0.0
0.01100
0.00542
0.48
0.66
DR+CPP
DR+CPP
DIA1
DIA2
DIA3
DIA4
DIA5
DIA6
DIA7
0.036
0.032
0.034
0.021
0.021
0.022
0.021
51.9
30.3
30.4
46.0
48.1
75.0
54.5
22.2
43.9
39.1
28.0
29.6
12.5
18.2
22.2
22.7
21.7
26.0
18.5
12.5
22.7
3.7
3.0
8.7
0.0
3.7
0.0
4.5
0.01248
0.01123
0.01485
0.00744
0.00606
0.00312
0.00773
0.53
0.54
0.52
0.61
0.45
0.38
0.32
DR/7dDIA
DR/7dDIA
DR/7dDIA
DR/7dDIA
DR/7dDIA
DR/7dDIA
DR/7dDIA