BBSG-503 Spring Semester,
2008
SECTION 9: SIGNALING
SECTION DIRECTOR: JJ Baldassare, Ph.D.
LOCATION: LRC Classrooms 105 A&B
LECTURE 1
Monday, February 25, 2008 9:00-10:00AM
LECTURE TITLE: Cell Communication
Lecturer: Dr. JJ Baldassare
Reading: Signal Transduction, Gomberts et. al. pages 20 to 32
Overview of cell signaling
Cell communication
Ligands- first messengers
Receptors
LECTURE 2
Tuesday, February 26, 2008 9:00-10:00AM
LECTURE TITLE: Extracellular Matrix
Lecturer: Dr. Jan Ryerse
Reading: Molecular Cell Biology, Lodish et al, 4th edition, (2000), pages 976-993
Topics to be covered:
collagens
hyaluronan and proteoglycans
glycosaminoglycans
laminin and fibronectin
ECM in signaling processes
LECTURE 3
Wednesday, February 27, 2008
9:00-10:00AM
LECTURE TITLE: Cell Adhesion Molecules: Selectins, Cams I
Lecturer: Dr. Jan Ryerse
Reading: Molecular Cell Biology, Lodish et al, 4th edition, (2000), pages 968-973
Topics to be covered:
classes of matrix-cell and cell-cell adhesion molecules
structure/function of cadherins and integrins
LECTURE 4
Thursday, February 28, 2008
9:00-10:00AM
LECTURE TITLE: Cell Adhesion Molecules: Selectins, Cams II
Lecturer: Dr. Jan Ryerse
Reading: Molecular Cell Biology, Lodish et al, 4th edition, (2000), pages 968-973
Topics to be covered:
classes of matrix-cell and cell-cell adhesion molecules
structure/function of cadherins and integrins
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LECTURE 5
Friday, February 29, 2008 9:00-10:00AM
LECTURE TITLE: Cell Adhesion Molecules: Selectins, Cams
Lecturer: Dr. Jan Ryerse
Reading: Molecular Cell Biology, Lodish et al, 4th edition, (2000), pages 968-973
Topics to be covered:
Structure/function of selectins
CAMs in leukocyte extravasation
CADs in signaling cascades
cadherin and cancer
LECTURE 6
Monday, March 3, 2008
9:00-10:00AM
LECTURE TITLE: Ligand Receptor
Interactions
Lecturer: Dr. JJ Baldassare
Receptor binding fundamentals
Schatchard Plots- Linear, nonlinear
Log Plots
LECTURE 7
Tuesday, March 4, 2008
9:00-10:00AM
LECTURE TITLE: Tyrosine Kinase Receptors
I
Lecturer: Dr. JJ Baldassare
Reading: Signal Transduction, Gomberts et. al.
Chapter 11
Growth factor receptors-Receptor structure
Activation-dimerization
Tyrosine motifs and their role in signaling
Effectors/adaptors-Ras, PI 3-Kinase, PI PLCγ
LECTURE 9
Wednesday, March 5, 2008 9:00-10:00AM
LECTURE TITLE: G-protein Receptors I
Lecturer: Dr. JJ BaldassareReading: Signal Transduction,
Gomberts et. al. Chapter 4 and Molecular Cell Biology, Lodish et. al. Chapter 15 pages 852870
Overview of family
Structure
Heterotrimeric G-protein structure
GTP cycle
Signaling mechanisms & downstream
effectors
Adenylate cyclase and cAMP
PI PLC cycle
PI PLC and PIP2 hydrolysis & release of IP3
Calcium signaling
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LECTURE 10
Thursday, March 6, 2008
9:00-10:00AM
LECTURE TITLE: G-protein Receptors II
Lecturer: Dr. JJ BaldassareReading: Signal Transduction, Gomberts et. al. Chapter 4 and
Molecular Cell Biology, Lodish et. al. Chapter 15 pages 852-870
Nature of binding to receptors
Specificity in Gβγs coupling
Desensitization, down regulation
LECTURE 11
Friday, March 7, 2008
9:00-10:00AM
LECTURE TITLE: Tyrosine Kinase Receptors II Cross Talk between G-protein & Tyrosine
Kinase Receptors
Lecturer: Dr. JJ Baldassare
Readings: Trends in Endocrinology and Metabolism
Volume 16, Issue 1 , January-February 2005, Pages 26-33
MPP independent
MPP dependent
Adams proteins
EGFR transactivation
FGFR transactivation
LECTURE 12
Monday, March 10, 2008
9:00-10:00AM
LECTURE TITLE: Lipid Signaling
Lecturer: Dr. J McHowat
Reading: The membrane and lipids as integral
participants in signal transduction: lipid signal
transduction for the non-lipid biochemist, K.M.
Eyster, Advances in Physiology Education, 2007, vol
31, pp 5-16
Topics to be covered:
Phospholipases involved in intracellular signaling
Lipid precursors and the second
messengers
generated from them
LECTURE 13
Tuesday, March 11, 2008
9:00-10:00AM
LECTURE TITLE: Calcium Signaling I
Lecturer: Dr. J McHowat
Reading: Controlling Calcium Entry, C.W. Taylor, Cell 2002, vol 111, pp 767-769
Topics to be covered:
Changes in cytosolic calcium concentration
Measurement of intracellular calcium
Effects of elevated calcium
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LECTURE 14
Wednesday, March 12, 2008
9:00-10:00AM
LECTURE TITLE: MAP Kinases
Lecturer: Dr. JJ Baldassare
Readings: Widmann et al “Mitogen-Activated Protein Kinase:Conservation of a Three
Module from Yeast to Human” only pages 156-167
Linear signaling pathways signaling
Map kinases -linear signaling pathways, targets
Precessive vs Distributive
STUDY DAY
Thursday, March 13, 2008
SECTION 9 EXAM
Friday, March 14, 2008
No class
9:00 - noon
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