A
kDa
D44A Crg1
er
dd
La
MW
#2
#5
#4
#3
w
t
(c Cr
om g1
bi
n
E1 ed
0
(c 5 fra
om A_ ct
bi D1 ion
ne 0 s
d 8A 2,3
fra C ,4
ct rg )
io 1
ns
2,
3,
4)
Figure S4
~70
~55
-Crg1
~35
~27
B
C
100000
30000
Complete reaction
No substrate control
No SAM Control
No enzyme control
cantharidin
(m/z=197.0814)
Counts per second
Counts per second
120000
80000
60000
40000
20000
0
5
10
D
16000
Counts per second
15
20
Minutes
25
30
20000
15000
10000
0
35
0
5
10
E
Complete reaction
No substrate control
No SAM Control
No enzyme control
Methyl cantharidin
(m/z=211.0970)
14000
12000
10000
8000
6000
4000
2500
15
20
Minutes
30
Complete reaction
No substrate control
No SAM Control
No enzyme control
Hydrated methyl
cantharidin
(m/z=229.1076)
2000
25
1500
1000
500
2000
0
25000
5000
Counts per second
0
Complete reaction
No substrate control
No SAM Control
No enzyme control
Hydrated cantharidin
(m/z=215.0919)
0
5
10
15
20
Minutes
25
30
35
0
0
5
10
15
20
Minutes
25
30