Version: 2
Revision Date: 02/04/2016
Purified anti-Clathrin Heavy Chain (CLTCL2) Antibody
(Previously Covance catalog# MMS-427P)
Catalog# / Size
813901 / 100 µl
Clone
TD.1 (See other available formats)
Other Names
Clathrin heavy chain 1, clathrin, heavy polypeptide-like
2, clathrin heavy chain on chromosome 17
Previously
Covance Catalog# MMS-427P
Isotype
Mouse IgG1
Ave. Rating
★★★★★ 0 reviews
Description
Clathrin consists of three heavy chains (192 kD) and three light chains (LCa
or LCb, 25-29 kD) and self assembles into a polyhedral cytoskeletal element.
The self assembly facilitates clathrin's central role in membrane sorting and
selective transport within the cell.
Reactivity
Human, Bovine
MeOH fixed HeLa cells double stained with
Clathrin, Clone TD.1, and Gaintin (Cat. No.
924302). Cells were counterstained with
DAPI.
Immunogen
This monoclonal antibody was raised using the globular N-terminal domain of clathrin heavy
chain.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the
storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You
can also check your vial or your CoA to find the most accurate storage condition for this
antibody.
Application
WB, IF
Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1:250*
• IF: 1:200**
Application Notes
This antibody is effective in immunoblotting (WB) and immunofluorescence (IF)**.
*Predicted MW = 190 kD
**Recommend using methanol fixation for IF.
This antibody reacts with clathrin heavy chains (CHC). The antibody is useful for
immunoblotting of clathrin heavy chains from cow CHC and immunofluorescence. It does not
react with native clathrin in immunoprecipitation.
Application References
(PubMed link indicates
BioLegend citation)
1. Hood FE, Royle SJ. Functional equivalence of the clathrin heavy chains CHC17 and CHC22
in endocytosis and mitosis. J. Cell Sci. 122:2185-2190, Jul 2009. [IF] PubMed
2. Jiang R, Gao B, Prasad K, Greene LE, Eisenberg E. Hsc70 chaperones clathrin and primes it
to interact with vesicle membranes. J Biol Chem 275(12):8439-8447, 2000.
3. Nathke IS, Heuser J, Lupas A, Stock J, Turk CW, Brodsky FM. Folding and trimerization of
clathrin subunits at the triskelion hub. Cell 68:899-910, 1992.
4. Jackson AP, Seow H-F, Holmes N, Drickamer K, Parham P. Clathrin light chains contain brainspecific insertion sequences and a region of homology with intermediate filaments. Nature
326:154-159, 1987.
Publication Library
RRID
AB_2564779 (BioLegend Cat. No. 813901)
Antigen Details
Gene ID
1213
UniProt
View information about Clathrin Heavy Chain (CLTC) on UniProt.org
Documentation
Technical data sheet
Certificate of Analysis
Safety Data Sheet
Related FAQs
There are no FAQs for this product.
Related Categories
NeuroscienceProducts > Protein Trafficking and Clearance > Protein Clearance Antibodies >
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