Int. J. Mol. Sci. 2016, 17, 511; doi:10.3390/ijms17040511
S1 of S2
Supplementary Materials: The Effects of Different
Purifying Methods on the Chemical Properties,
in Vitro Anti-Tumor and Immunomodulatory
Activities of Abrus cantoniensis
Polysaccharide Fractions
Shaowei Wu, Xiong Fu, Margaret A. Brennan, Charles S. Brennan and Chen Chun
0.8
5.0
A490
NaCl (mol/L)
4.5
4.0
0.6
AP-AOH30-2
0.5
AP-AOH30-1
3.0
0.4
2.5
0.3
2.0
0.2
1.5
NaCl (mol/L)
3.5
A490
0.7
0.1
1.0
0.0
0.5
-0.1
0.0
0
10
20
30
40
50
60
Fractions
Figure S1. Elution curve of crude polysaccharide AP-AOH30 purified by DEAE-52 cellulose
anion-exchange chromatography.
5.0
4.5
A490
NaCl (mol/L)
AP-AOH80-1
4.0
AP-AOH80-2
3.5
0.7
0.6
0.4
2.5
0.3
2.0
0.2
1.5
NaCl (mol/L)
0.5
3.0
A490
0.8
0.1
1.0
0.0
0.5
-0.1
0.0
0
10
20
30
40
50
60
Fractions
Figure S2. Elution curve of crude polysaccharide AP-AOH80 purified by DEAE-52 cellulose anionexchange chromatography.
Int. J. Mol. Sci. 2016, 17, 511; doi:10.3390/ijms17040511
S2 of S2
0.8
5.0
A490
NaCl (mol/L)
4.5
0.6
AP-ACl-1
AP-ACl-2
3.5
0.5
A490
3.0
0.4
2.5
0.3
2.0
0.2
1.5
NaCl (mol/L)
4.0
0.7
AP-ACl-3
0.1
1.0
0.0
0.5
-0.1
0.0
0
20
40
60
80
100
Fractions
Figure S3. Elution curve of crude polysaccharide AP purified on the DEAE-sepharose fast
flow column.