LESSON PLAN Course: Basic Breath Test Operator Class Title: Principles and Theory Effective Date: April 21, 2014 1 COVER PAGE TIME: COURSE : CLASS TITLE: CLASS LEVEL: OBJECTIVES: 90 Minutes Basic Breath Test Operator Principles and Theory Basic Recruit See page 3 METHOD OF INSTRUCTION: METHOD OF TESTING: TRAINING AIDS: Lecture Written Computer, Proxima, Screen, IR Light Sources, Sample Chambers, Filter Wheel, Detector Block, Student Manual REFERENCES: See page 4 COMPLETED BY: DATE: REVISIONS BY: DATE OF REVISIONS: Thomas G. Blankenship October 2010 Danny Dailey April 21, 2014 APPROVED BY (signature): DATE APPROVED: 2 OBJECTIVES: AT THE END OF THE SESSION EACH LEARNER WILL BE ABLE TO: 1. Without reference, as discussed in class explain how an Intoxilyzer Breath Analysis Instrument quantifies and qualifies a breath sample through the use of the infrared (IR) absorption principle. 3 REFERENCES: 1. Ultraviolet-Visible Absorption Spectroscopy Booklet Memphis State Univ.; 2. Tests for BAC in Highway Safety and Expert Testimony Course Notebook 3. Center for Law in Action Indiana Univ. 1996; 4. Intoxilyzer 5000EN and 8000 Operators Manuals 5. NASA Observation Education-Reference Module Web Site 6. Journal of Motor Vehicle Law, 1994. 4 LEC: INTRO The purpose of this block of instruction is to explain in simple terms the principle on how the instrument collects a breath sample and determines an alcohol concentration level. The intent of this instruction is not to qualify you as an instrument technician but rather give you a basis for understanding of how the instrument provides a breath analysis. This is your initial instruction on how the instrument performs that will establish the foundation for further training you will receive this week in the operation of the Intoxilyzer 5000EN and the 8000. 5 NTF Instructors should begin each presentation with a listing of all objectives for the block of instruction. LEC Without reference, the learner will be able to explain how an Intoxilyzer Model 5000EN and the 8000 Breath Analysis Instrument quantitatively measures a breath sample through the use of the Infrared (IR) Absorption Principle, as discussed in class. 6 LEC Specifies that operators will receive 40 hours of training. Part of the training is specified as, Physiological effect of alcohol (Alcohol and the Human Body); Operation of approved instruments (Introduction and Initial Operation of the BT Instruments); General instrument theory. You will see during moot court, at the end of the week, how to use some of this theory to improve your testimony. Operation of the instrument will be tested on Thursday, you will have run a minimum of 10 tests prior to Thursday. 7 LEC The Intoxilyzer uses infrared light to measure the amount of alcohol molecules in a breath sample after the subject provides an adequate sample. 8 LEC In 1971 instruments begin using the infrared absorption principle to determine breath alcohol results. The Intoxilyzer 5000EN & 8000 uses a law of chemistry and physics known as the Lambert-Beer Law to determine the alcohol concentration in a breath sample. This law states that the amount of energy absorbed by a particular substance is proportional to the number of absorbing molecules in the sample. The amount of infrared energy absorbed in a breath sample is proportional to the amount of alcohol present in a breath sample introduced into the instrument sample chamber. The Intoxilyzer reports the measured alcohol concentration in grams of alcohol per 210 liters of breath. 9 Electromagnetic Spectrum W A VEL EN G T H T Y P E O F R A D A T IO N EN ER G Y WA VEL EN GTH LON G W AV E LE N GT H T Y PE OF R A D A T ION RADI O W AV E S MI CRO W AV E S I NF RARE D LI GH T EN ER G Y L OW E NE R GY V IS I BLE LI GH T RE REDD OR OR ANGE ANGE YYEELLLLOW OW GRE GREEE NN BL BLUE UE IND IND IG IGO O S HOR T W AV E LE N GTH LO N G W A V E LE N G T H ____ ____ __VVIIOLE OLE TT__ ____ _____ ___ U L TRAV I OLE T X -RAY GAM MA RAY S HI GH E NE R GY RADI O W AVES MI C R O W A V E S I N F R A R E D LI G H T L OW E NE R GY V IS I B LE LI G H T RREEDD OORR AANNGGEE YYEELL LL OOW W GGRREEEE NN BBLL UUEE IN INDD IG IG OO S H O R T W A V E LE N G TH __ ____ ____VVI I OOLE LE TT ____ ____ ______ U L TR A V I O LE T X -R A Y G A M MA R A Y S WAVEL ENGTH LON G WAVE LEN GT H SHOR T WAVE LEN GTH T Y PE OF RADAT ION RADI O WAVES MI CRO WAVES I NF RARED LI GH T VISI BLE LI GH T RED RED OR ORANGE ANGE YEL YELLLOW OW GREE GREE NN BL BLUE UE IND INDIG IGO O ____ ____ __VI VIOLE OLE TT__ ____ _____ ___ U L TRAVI OLE T X-RAY GAM MA RAYS ENERG Y L OW ENER GY HI GH ENE R GY HI GH E NE R GY 9 LEC Infrared is a specific form of energy in the spectrum of electromagnetic radiation. "Infrared" (IR) means "below red“ and is a region of the electromagnetic spectrum with longer wavelength (lower energy) than visible red light, and thus is invisible to the human eye. Wavelengths of electromagnetic radiation between 0.7 and 1,000 micrometers are considered infrared. Light visible to the human eye occurs at wavelengths between about 0.7 and 0.4 micrometers. A micrometer is a unit of length which is one-millionth of it meter and is commonly referred to as a micron. 10 INFRARED ABSORPTION ETOH C2 H5 OH 12 LEC A molecule such as ethyl alcohol has a unique structure consisting of carbon, hydrogen and oxygen atoms. Alcohol molecules when exposed to a particular IR light wave length will begin to resonate/vibrate/flex at the carbon & hydrogen bonds. Alcohol was discovered to occur significantly at the IR wavelength of 3.48 micro farads. To bend/flex in such a manner requires energy. The energy to do this comes from the IR light. The result is that the amount of IR light is reduced due to the absorption. This is the Infrared Absorption Principle. 11 LEC The instrument takes a measurement of light before alcohol is introduced into the sample chamber. This happens on the 5000EN when the “….” are going across the digital display. On the 8000 you will see solid square boxes going right to left on the screen. Alcohol molecules are then exposed to the infrared light. This is the only place in the instruments where ETOH and IR light meet. Alcohol molecule bonds start to bend & conform to specific IR wavelength, approximately 3.48 microfarads, using energy from the IR light. Decrease in infrared light energy is detected. The decrease is converted to BrAC. 12 LEC The instrument must establish initial readings from each filter or detector. The reference reading. On the 5000EN when the operator sees …………. displayed across the digital display the instrument is validating its operational settings and establishing a reference using the clear sample chamber. On the 8000 when the operator sees displayed across the display the instrument is validating its operational settings and establishing a reference using the clear sample chamber. NTF This is the reading the instrument uses to compare to the reading after the Air Blanks, Calibration Check, and Subjects Test. 13 OPTICAL BENCH IR to •Converts electrical energy Quartz-Iodide Lamp Sample Chamber Filter Wheel 81.4cc or ml at 46 +/- 1 Detector Ethanol Acetone/ETOH 2nd Lens 1st Lens Toluene/ETOH Methanol/ETOH Interrupter H2O 14 LEC Optical Bench of the 5000EN Quartz-Iodide Lamp – This is the infrared light source. It projects IR and visible light. NTF LEC NTF Show the class the IR Light Source Sample Chamber – This is the location where the breath sample comes in contact with the IR light to be analyzed. The size of the sample chamber is 81.4 ml or cc. The temperature is 46˚ C +/- 1˚ C. Show the class the Sample Chamber. 14 LEC Lens – There are two lens, one at each end of the sample chamber. The first lens (closest to the IR Source) disperse the light throughout the chamber. The second lens pinpoints the remaining light and directs it to the filter wheel. NTF Point out the lens, they do unscrew to make it easier to see. LEC NTF LEC We will discuss the filter wheel in length on the next slide. Note where it is located, again the remaining IR light is directed through the filter wheel and that light is measured by the detector. Show the filter wheel mounted to the detector board. The copper looking disc located between the filter wheel and the board is the detector. The black U shaped piece that goes around the filter wheel is the Interrupter. The Interrupter identifies which filter the detector is reading. The filter wheel spins at 2800 RPMS. The Detector converts the IR light to electrical energy which is converted to a BrAC. 15 FILTER WHEEL Ethanol Acetone/ETOH Water Vapor Methanol/ETOH Toluene/ETOH 15 Filter Wheel LEC The instrument is not specific to ethanol. It can detect other substance, which can give the appearance of alcohol intoxication. Acetone/ethanol filter –Diabetics experiencing a medial emergency may appear to be intoxicated. A subject in a severe high blood sugar diabetic emergency will express high levels of the substance acetone in their breath. Acetone is a by-product of the body’s attempt to reduce high levels of sugar building up in the blood. While the subject is blowing into the instrument, it is comparing the light energy detected from the ethanol filter against the light energy detect from the acetone/ethanol filter. 16 LEC Methanol/ethanol and the Toluene/Ethanol Filter – This filter helps detect substances that are usually found in inhalant type products. Although these filters are labeled as methanol and toluene there is other impairing inhalants that are similar in molecular structure that will also absorb IR energy at these specific wavelengths. While the subject is blowing into the instrument, it is comparing the light energy detected from the ethanol filter against the light energy detected from the methanol/ethanol filter and the toluene/ethanol filter. When analyzing the breath sample and the comparison is made if there is a significant difference in the electrical readings at the detector, the instrument will respond not with a alcohol reading but with an “Interferent Detected” card. 17 LEC There are no moving parts such as the filter wheel, in the 8000, it has two preheat chambers and one chamber that measurements are taken. The infrared light source is pulsed, this essentially takes the place of the filter wheel in 5000EN. A series of check valves is used to direct air, breath, wet bath, or compressed gas through the instrument. The sample chamber size is 29ml/cc and the temperature is 47 degrees Celsius. NTF Show the sample chamber, light source, and detector block. 18 Ethanol Infrared Spectra with notation for 8000 IR filters 100% 95% 90% Percent of Absorption Detected 85% 80% 75% 70% 65% 60% 55% 50% 45% 40% 35% 3.4 micron detector 30% 25% Interferents 20% 15% 9.4 micron detector 10% 5% 0% 3.0 4.0 5.0 6.0 7.0 8.0 9.0 ETOH 10.0 11.0 Wavelength (microns) LEC What you are seeing in this slide is the IR light remaining at that particular micron range. The 100% at the top of the graph would be the reference point. If there was a significant difference between these two detectors the 8000 would flag the test as an interferent detected. The big difference between the 8000 measurement and the 5000EN measurement is the 8000 measurement is taken at the bond between particular atoms in the ETOH molecule. 19 LEC We do not know what specific number is an indicator of a “Significant Difference” but for the sake of demonstration we will use the value of .010. Significant Difference Example: Ethanol Filter = .100BrAC Acetone/ethanol Filter = Difference between filters = .110BrAC .010BrAC When comparing filter results, and there is an insignificant difference between the ethanol filter and the other filters, the instrument will disregard what it sees in the other filters and give the operator the true ethanol filter reading. 20 LEC Insignificant Difference Example: Ethanol Filter = .100 BrAC Acetone/ethanol Filter = Difference between filters = .107BrAC .007 BrAC Impairing products that may contain methanol or toluene can cause the absorption of infrared light. These are inhalants such as paint (gold/silver), glue, gasoline, butane, duster, nail polish remover, and such. QTC What are you hearing in your area that people are huffing? AR Responses will vary. LEC When an Interferent is detected, the instrument will print a card indicating that an Interferent was detected, but it will not identify nor specify which filter detected it. Your actions as an operator will be discussed in a later lecture. 21 LEC For the operator to get a completed test the subject must meet these parameters during the “Please Blow” Mode. Time – The subject must blow continuously for a “minimum” of four seconds. If the instrument detects alcohol on the sample it will require more than four seconds. Pressure – the subject must blow hard enough so that the flow sensor detects a greater than a .15 liters or 150 milliliters per second of steady flow. 22 LEC Volume – The subject must provide a minimum of 1.1 liters or 1100 milliliters, remember you want the deep lung air to get an accurate reading of the BrAC. Slope – The slope parameter is required to determine if the reading is from residual mouth alcohol. The subject must provide a homogenous concentration of alcohol for the instrument to read the BrAC. The alcohol level must plateau. NTF Remember the subject must meet all of these parameters to satisfy the requirement for a completed breath test. If the subject’s pressure drops below the requirement all of the parameters will restart from the point when the pressure is back to the minimum requirement. 23 LEC Deficient Sample If the subject fails to meet the parameters of time volume, or pressure in the three (3) minute please blow mode the instrument will produce a deficient sample evidence card. 24 Normal Breath Test BrAC .100 Plateau .050 .000 0 1 2 3 4 Time (Sec) 23 LEC 21 You know you are getting a good sample when the subject is blowing you hear a constant/steady tone and the words “Please Blow” are constantly on the screen (not flashing). At the please blow mode the operator has three (3) minutes to gather a sufficient sample for analysis. If the subject fails to meet all the parameters the tone will stop and the words “Please Blow” will flash. The operator should instruct the subject that they need to continue to blow until you tell them to stop. 25 Slope parameter is activated Drop is due to detection of Residual Mouth Alcohol BrAC .100 .050 INVALID SAMPLE .XXX .020 .000 0 1 2 3 4 5 6 Time (10ths of a Second) LEC 7 8 24 Slope Parameter Activated If residual mouth alcohol is present while the subject is blowing the slope parameter will detect a drop of alcohol in a given time. The instrument will print a card indicating an “Invalid Sample”, next to subjects test will be .XXX. NTF The operators response will be discussed in later lecture. 26 Final Review QTC What is the operating principle of both the Intoxilyzer 5000EN and the 8000? AR Infrared Absorption Principle QTC What is the principle of the Lambert-Beer Law? AR IR energy absorbed in the breath sample is proportional to the amount of alcohol introduced into the sample chamber. QTC What is the size of the sample chamber for the 5000EN and the 8000? AR 5000EN/81.4ml………8000/29ml 27 Final Review (cont) QTC What is the operating temperature of the 5000EN and the 8000? AR 5000EN/46+or-1 degrees C….8000/47 degrees C QTC These instruments are specific to alcohol only. AR False QTC What other substances does the 5000EN/8000 detect? AR Methanol, Toluene, Acetone, Water QTC What are the parameters for the 5000EN/8000? AR Time: minimum 4 seconds Pressure: constant rate of at least 150 ml per second Volume: total of 1100 ml of air passing through Slope: reaches a plateau or homogeneous plane QTC AR QTC AR What will be the result if all parameters are not met? Deficient sample (with or without alcohol) What is the result should the slope parameter be activated? Invalid sample.XXX (Residual Mouth Alcohol) TIME: 88 MINUTES 28 Concluding Statement: LEC You have now received training as to the basic operating principles of the Intoxilyzer 5000EN and the Intoxilyzer 8000. This information will help you understand the inner workings and processes by which breath alcohol concentration levels are calculated. You will be better prepared for courtroom testimony as to those operating principles. It will also prepare you for your next block of training involving the actual operation of each instrument. AFQ TIME: 90 MINUTES 29
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