ISO9001
For research use only
ISO9001
For research use only
Ava III
Ava III
Product
Quantity
Cat. No.
Remarks
Ava III
1,000 unit
EBR-1023
10 unit/ml
Product
Quantity
Cat. No.
Remarks
Ava III
1,000 unit
EBR-1023
10 unit/ml
Recognition site
Recognition site
5′…A T G C A T…3′
3′…T A C G T A…5′
5′…A T G C A T…3′
3′…T A C G T A…5′
Source
Source
Anabaena variabilis.
Anabaena variabilis.
Reaction Conditions
Reaction Conditions
Buffer 4. 20 mM Tris-acetate (pH 7.9), 50 mM potassium acetate, 10 mM magnesium acetate,
Buffer 4. 20 mM Tris-acetate (pH 7.9), 50 mM potassium acetate, 10 mM magnesium acetate,
1 mM DTT. Incubate at 37℃.
1 mM DTT. Incubate at 37℃.
Concentration & Storage Condition
Concentration & Storage Condition
Store at -20℃. 10 unit/ml in 10 mM Tris-HCl (pH 7.5), 200 mM KCl, 1 mM DTT, 1 mM EDTA,
Store at -20℃. 10 unit/ml in 10 mM Tris-HCl (pH 7.5), 200 mM KCl, 1 mM DTT, 1 mM EDTA,
200 mg/ml BSA, 50% (v/v) glycerol.
200 mg/ml BSA, 50% (v/v) glycerol.
Heat Inactivation Condition
Heat Inactivation Condition
65℃ for 20min
65℃ for 20min
Enzyme dilution
Enzyme dilution
Dilute with Storage Buffer.
Dilute with Storage Buffer.
QC Tests
QC Tests
Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity,
Activity, ligation and recutting, exo and endonuclease activity test, SDS-PAGE purity,
performance tests.
performance tests.
Methylase Sensitivity
Methylase Sensitivity
Not sensitive to dam, dcm or CpG methylation.
Not sensitive to dam, dcm or CpG methylation.
Buf4
BUFFER
37℃
REACTION
65℃
INACTIVATION METHYL BLOCK
BLUE/WHITE
Buffer
1
2
3
4
Buf4
Activity
0
50
25
100
BUFFER
37℃
REACTION
65℃
INACTIVATION METHYL BLOCK
BLUE/WHITE
Buffer
Activity
1
0
2
50
3
25
4
100
㈜ 엘피스바이오텍
[302-854] 123-12 Chunglim-Dong, Seo-Gu, Taejeon, Korea
㈜ 엘피스바이오텍
[302-854] 123-12 Chunglim-Dong, Seo-Gu, Taejeon, Korea
www.elpisbio.com
Tel: +82-42-581-8448. Fax: +82-42-581-8449
www.elpisbio.com
Tel: +82-42-581-8448. Fax: +82-42-581-8449