New England Biolabs, Inc.
Tel: 800-632-5227 (orders)
Tel: 800-632-7799 (support)
Fax: 978-921-1350
[email protected]
www.neb.com
DNA & RNA Ladders
Conventional Ladders and PCR Marker
TriDye™ DNA Ladders
1 kb DNA Ladder
#N3232S
#N3232L
100 µg . . . . . $58 (USA)
500 µg . . . . $232 (USA)
TriDye 2-Log DNA Ladder
#N3270S 125 gel lanes (1.25 ml) . . . . . $79 (USA)
100 bp DNA Ladder
#N3231S
#N3231L
50 µg . . . . . $58 (USA)
250 µg . . . . $232 (USA)
2-Log DNA Ladder
#N3200S
#N3200L
100 µg . . . . . $58 (USA)
500 µg . . . . $232 (USA)
Quick-Load™ DNA Ladders
50 bp DNA Ladder
#N3236S
#N3236L
100 µg . . . . . $58 (USA)
500 µg . . . . $232 (USA)
Quick-Load 1 kb DNA Ladder
#N0468S 125 gel lanes (1.25 ml) . . . . . $58 (USA)
Low Molecular Weight DNA Ladder
#N3233S
50 µg . . . . . $58 (USA)
#N3233L
250 µg . . . . $232 (USA)
Quick-Load 100 bp DNA Ladder
#N0467S 125 gel lanes (1.25 ml) . . . . . $79 (USA)
PCR Marker
#N3234S
#N3234L
Features
TriDye 1 kb DNA Ladder
#N3272S 125 gel lanes (1.25 ml) . . . . . $58 (USA)
TriDye 100 bp DNA Ladder
#N3271S 125 gel lanes (1.25 ml) . . . . . $79 (USA)
Quick-Load 2-Log DNA Ladder
#N0469S 125 gel lanes (1.25 ml) . . . . . $79 (USA)
30 µg . . . . . $58 (USA)
150 µg . . . . $232 (USA)
❚
Uniform Band Intensities
❚
No Extraneous High Molecular Weight Bands
Value – Load Only 0.5 µg/lane of 1 kb, 100 bp or Low Molecular
Weight DNA Ladders and 0.3 µg/lane of PCR Marker
ssRNA Ladder
#N0362S
25 µg . . . . . $58 (USA)
❚
dsRNA Ladder
#N0363S
25 µg . . . . . $75 (USA)
❚
Ladders are Available in Bulk Quantities
siRNA Marker
#N2101S
❚
Easy-to-identify Reference Bands
4.5 µg . . . . . $58 (USA)
1 kb DNA Ladder
100 bp DNA Ladder
ng
kb
10.0 42
8.0
42
6.0
50
5.0
42
4.0
33
3.0 125
2-Log DNA Ladder
ng
bp
ng
kb
1,517
45
1,350 103
1,200
35
10.0 40
8.0 40
1,000
900
800
700
600
95
27
24
21
18
6.0
5.0
48
40
916
70
4.0
32
766
700
650
600
550
500
450
400
350
58
54
50
46
42
76
34
31
27
300
46
250
57
200
107
3.0 120
500/517 97
2.0
48
1.5
36
1.0
42
400
38
300
29
25
200
48
100
0.5 µg on 1.3%
TAE agarose gel
0.5
0.5 µg on 0.8%
TAE agarose gel
Technical Bulletin
(10/5/05)
42
50 bp DNA Ladder
40
1.5
57
1.2
45
1.0
0.9
0.8
0.7
122
34
31
27
0.6
23
ng
150
46
100
69
dsRNA Ladder
bp
9,000
500 27
500
7,000
350 20
300 33
200 110
bp
ng
766
62
500
40
300
48
150
61
50
89
0.4
49
0.3
37
0.2
32
0.1
61
1.0 µg on 1.0%
TBE agarose gel
300
84
1.0 µg on 1.8%
TBE agarose gel
3,000
2,000
80
1,000
150 33
100 43
50
75
30
58
21
50
63
500
1 µg on 1.0%
TBE agarose gel
siRNA Marker
bp ng
25 15
21 15
25
0.5 µg on 1.8%
TBE agarose gel
xylene
cyanol FF
5,000
150
1.5 µg on 2.0%
TBE agarose gel
50
ssRNA Ladder
bases
250 27
0.5 124
PCR Marker
0.3 µg on 1.8%
TBE agarose gel
2.0
bp
Low Molecular
Weight DNA Ladder
bp ng
766 42
43
17 15
45 ng/lane
20% TBE polyacrylamide gel
TriDye™ Ladders: 1 kb, 100 bp and 2-Log DNA Ladders available in
ready-to-load format containing xylene cyanol FF, bromophenol blue
bromophenol and orange G.
blue
Quick-Load™ Ladders: 1 kb, 100 bp and 2-Log DNA Ladders available
orange G
in ready-to-load format containing bromophenol blue.
(See other side)
DNA & RNA Ladders (continued)
Description:
The 1 kb DNA Ladder has a number of proprietary plasmids
that are digested to completion with appropriate restriction
enzymes to yield 10 bands suitable for use as molecular
weight standards for agarose gel electrophoresis. The
digested DNA includes fragments ranging from 0.5–10.0 kb.
The 3.0 kb fragment has increased intensity to serve as a
reference band.
The dsRNA Ladder is a set of 7 annealed double-stranded RNA
molecules produced by in vitro transcription of 14 linear DNA
templates. The ladder sizes are: 500, 300, 150, 80, 50, 30 and
21 base pairs. All have 2-base, 3´ extensions. The 80 base pair
fragment is at double intensity to serve as a reference band.
This ladder is suitable for use as a size standard in dsRNA and
RNAi analysis on both non-denaturing polyacrylamide and
agarose gels.
The 100 bp DNA Ladder has a number of proprietary
plasmids that are digested to completion with appropriate
restriction enzymes to yield 12 bands suitable for use as
molecular weight standards for agarose gel electrophoresis.
The digested DNA includes fragments ranging from
100–1517 bp. The 500 and 1,000 base pair fragments have
increased intensity to serve as reference points.
The siRNA Marker contains three synthetic annealed siRNA
duplexes that are 17, 21 and 25 base pairs in length. All have 2
base 3´ overhangs.
The 2-Log DNA Ladder has a number of proprietary plasmids
that are digested to completion with appropriate restriction
enzymes to yield 19 bands suitable for use as molecular
weight standards for agarose gel electrophoresis. This
digested DNA includes fragments ranging from 100 base
pairs (bp) to 10 kilobases (kb). The 0.5, 1.0 and 3.0 kb bands
have increased intensity to serve as reference points.
The Quick-Load DNA Ladders are pre-mixed, ready-to-load
molecular weight markers containing bromophenol blue as a
tracking Dye.
The 50 bp DNA Ladder has a number of proprietary plasmids
that are digested to completion with appropriate restriction
enzymes to yield 17 bands suitable for use as molecular
weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 50–1350 bp. The
200 and 500 base pair fragments have increased intensity to
serve as reference points.
The Low Molecular Weight DNA Ladder has a number of
proprietary plasmids that are digested to completion with
appropriate restriction enzymes to yield 11 bands suitable
for use as molecular weight standards for both agarose
and polyacrylamide gel electrophoresis. The digested DNA
includes fragments ranging from 25–766 bp. The 200 base
pair band has increased intensity to serve as reference
points.
The PCR Marker consists of 5 double-stranded DNA bands
that are suitable for use as molecular weight standards
for agarose and acrylamide gel electrophoresis. The PCR
Marker includes fragments ranging from 50–766 bp.
The ssRNA Ladder is a set of 7 RNA molecules produced by
in vitro transcription of a mixture of 7 linear DNA templates. The ladder sizes are: 9000, 7000, 5000, 3000, 2000,
1000 and 500 bases. The 3000 base fragment is at double
intensity to serve as a reference band. This ladder is suitable
for use as an RNA size standard on denaturing or native
agarose gels.
The TriDye DNA Ladders are pre-mixed, ready-to-load molecular weight markers containing 3 dyes which serve as visual
aids to monitor the progress of migration during agarose gel
electrophoresis.
Storage Conditions: DNA Ladders and the ds RNA Ladder are
supplied in 10 mM Tris-HCl (pH 8.0), 1.0 mM EDTA. For long
term storage store at –20°C. All DNA Ladders and PCR Marker
are stable for at least 3 months at 4°C.
ssRNA Ladders are supplied in 20 mM K0Ac (pH 4.5). Store at
–70°C. For short term storage (< 1 week), ladder can be stored
at –20°C.
siRNA Markers are supplied in 3% Ficoll 400, 1 mM EDTA and
0.06% orange. Store at –20°C.
Concentration: 500 µg/ml (1 kb, 100 bp, dsRNA Ladder and
Low Molecular Weight) 1,000 µg/ml (2-Log and 50 bp), 300
µg/ml (PCR Marker), 500 µg/ml (ssRNA Ladder) and 30 ng/µl
(siRNA Marker).
Recommended Load:
1 kb DNA Ladder: 0.5 µg
100 bp DNA Ladder: 0.5 µg
2-Log DNA Ladder: 1.0 µg
50 bp DNA Ladder: 1.0 µg
Low Molecular Weight DNA Ladder: 0.5 µg
PCR Marker: 0.3 µg
siRNA Marker: 45 ng
ssRNA Ladder: 1.0 µg
dsRNA Ladder: 1.5 µg
Reference:
1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual
Manual, (2nd ed.), (pp. 10.51–
10.67). Cold Spring Harbor: Cold Spring Harbor Laboratory
Press.
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