Microscope Notes
Types of Microscopes
1. Compound Light Microscope: allows _____light__ to pass through an image and
use two lenses to ____magnify____ object
2. Electron Microscope: Uses beams of ___electrons___ to form an image
- ____Transmission_______ Electron Microscope (TEM)
- ____Scanning_____ Electron Microscope (SEM)
a. TEM – Shines a beam of light through a thin species to form image
b. SEM – Shines a narrow beam of electrons on the surface to form image
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__Eyepiece____: contains a lens capable of 10x magnification
Revolving Nosepiece: ____turns___ to change from one power to another
___Low ___ power objective: attached to the revolving nosepiece and contains a
lens capable of 4x magnification
Medium power objective: attached to the revolving nosepiece and contains a lens
capable of 10x magnification
High power objective: attached to the revolving nosepiece and contains a lens
capable of 43x magnification
____Coarse ____ Adjustment: brings objects into rapid but coarse focus
___Fine _____ Adjustment: brings objects slowly into fine focus
_Mirror/lamp__: directs light into the scope adjustment
Stage clips: __holds__ glass slide and specimen in place
Stage Opening: allows __light___ to pass through the stage
___Diaphragm____: regulates the amount of light entering the scope
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Stage: supports slide
To calculate the power of magnification, _____multiply______ the ____power_ of
the ___ocular lens__ by the ___power___ of the objective lens
We can see better _____detail____ with higher powers of magnification, but we cannot
see as much of the image.
When using the microscope…
1 – Turn on the microscope and then rotate the nosepiece to click the red-banded
objective into place.
2 – Place a slide on the stage and secure it using the __stage clips______. Use the coarse
adjustment knob (____large__ knob) to get it the image into view and then use the fine
adjustment knob (_____small____ knob) to make it clearer.
3 – Once you have the image in view, rotate the nosepiece to view it under different
powers. Draw what you see on your worksheet!
Be careful with the largest objective! Sometimes there is not enough room and you
will not be able to use it!
4 – When you are done, turn off the microscope and put up the slides you used.
How to make a wet-mount slide…
1 – Get a clean slide and _____coverslip_____ from your teacher.
2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water
will run off the edge and make a mess!
3 – Place the edge of the cover slip on one side of the water drop.
4 - Slowly lower the _________coverslip______ on top of the drop.
5 – Place the slide on the stage and view it first with the red-banded objective. Once you
see the image, you can rotate the nosepiece to view the slide with the different objectives.