2 SI
B. L. Dumont and B. A. Payseur
A
Frequency
3000
More WSB-like
More PWD-like
More WSB-like
2000
1000
1000
0
0
-4
C
-2
0
2
4
6
-6
-4
D
CASTxWSB F1
More CAST-like
Frequency
WSBxPWD F1
2000
-6
More WSB-like
More PWD-like
-2
0
2
4
6
WSBxCAST F1
3000
3000
More CAST-like
More WSB-like
2000
2000
1000
1000
0
0
-6
-4
E
Frequency
B
PWDxWSB F1
-2
0
2
4
6
3000
2000
-2
0
2
4
6
PWDxCAST F1
More PWD-like
More CAST-like
-4
F
CASTxPWD F1
3000
-6
More PWD-like
More
CAST-like
2000
1000
1000
0
0
-6
-4
-2
0
2
4
6
-6
-4
-2
0
2
4
6
F1 Mean MLH1 Foci Count - Midparent Mean MLH1 Foci Count
FIGURE S1.—Mean MLH1 foci count in intersubspecific F1 males most closely resembles the paternal parent phenotype. We
used a bootstrapping randomization procedure (see main text) to derive an empirical distribution of differences between the F1
mean MLH1 foci count and the midparent average MLH1 foci count. If F1 animals equally resemble both parents, this
distribution should be centered on 0. In contrast, if F1 animals are more similar to the parent with higher (lower) mean MLH1
foci count, this distribution will be shifted to the right (left). For PWDxWSB (maternal x paternal parent) F1 animals, the
distribution is clearly shifted left, indicating that the F1s more closely resemble the low recombination rate WSB paternal parent
(A). In the reciprocal F1 cross, WSBxPWD F1 animals have mean MLH1 foci counts that more closely resemble those in the high
recombination rate PWD paternal strain (B). The distribution for CASTxWSB F1s is shifted to the right, as predicted if the mean
MLH1 foci count in the F1s is more similar to the WSB paternal parent phenotype (C). The WSBxCAST F1s have a mean
MLH1 foci count that is roughly equidistant between that of the two parents (D). Again, CASTxPWD F1s more closely resemble
the paternal strain (E), whereas the mean MLH1 foci count in PWDxCAST F1s is more similar to that of CAST (F).
B. L. Dumont and B. A. Payseur
A
Frequency
More
PWD-like
More
CZECHI-like
1500
PWDxCZECHI F1
2000
1000
1000
500
500
0
0
C
-2
-1
0
1
2
3
More
PERA-like
More
WSB-like
-3
D
PERAxWSB F1
2000
More
PWD-like
More
CZECHI-like
1500
-3
Frequency
B
PWDxCZECHI F1
2000
3 SI
-2
-1
0
1
2
3
WSBxPERA F1
2000
More
PERA-like
More
WSB-like
1500
1500
1000
1000
500
500
0
0
-3
-2
-1
0
1
2
3
-3
-2
-1
0
1
2
3
F1 Mean MLH1 Foci Count - Midparent Mean MLH1 Foci Count
FIGURE S2.—Mean MLH1 foci count in intrasubspecific F1 males most closely resembles the maternal parent phenotype. We
used a bootstrapping randomization procedure (see main text) to derive an empirical distribution of differences between the F1
mean MLH1 foci count and the midparent average MLH1 foci count. If F1 animals equally resemble both parents, this
distribution should be centered on 0. In contrast, if F1 animals are more similar to the parent with higher (lower) mean MLH1
foci count, this distribution will be shifted to the right (left). For PWDxCZECHI (maternal x paternal parent) F1 animals, the
distribution is shifted to the right, indicating that the F1s more closely resemble the higher recombination rate PWD maternal
parent (A). In the reciprocal F1 cross, CZECHIxPWD F1 animals have mean MLH1 foci counts that are more similar to those in
the lower recombination rate CZECHI maternal strain (B). The distribution for PERAxWSB F1s is shifted to the right, as
predicted if the mean MLH1 foci count in the F1s is more similar to the PERA maternal parent (C). The WSBxPERA F1s have a
mean MLH1 foci count that is roughly equidistant between that of the two parents (D).
4 SI
B. L. Dumont and B. A. Payseur
TABLE S1
NCBI GenBank sequence accession numbers
Species
IRBP Locus
CYTB Locus
Mus musculus castaneus
*
AY057805.1
Mus musculus musculus
**
AY057804.1
Mus musculus domesticus
***
AY057807.1
Mus spicilegus
AB125809.1
AY057809.1
Mus caroli
AB125797.1
AY057812.1
Mus spretus
AJ698883.1
AY057810.1
Rattus norvegicus
AJ429134.1
GU592997.1
Peromyscus maniculatus
AY163630.1
FJ800584.1
Microtus pennsylvanicus
AM919415.1
AF119279.1
Spermophilus tridecemlineatus
AF297278.1
AF157877.1
* Sequence pulled from whole-genome sequence of inbred mouse strain CAST/EiJ
** Sequence pulled from whole-genome sequence of inbred mouse strain PWK/PhJ
*** Sequence pulled from whole-genome sequence of inbred mouse strain WSB/EiJ
Whole genome sequences are available from the Wellcome Trust Sanger Institute at
http://www.sanger.ac.uk/resources/mouse/genomes/.
B. L. Dumont and B. A. Payseur
5 SI
TABLE S2
Synaptonemal complex lengths in murid rodents
Number of Cells
Mean Synaptonemal
Measured
Complex Length (μm)
CAST/EiJ
30
242.79
18.34
CIM
32
234.56
19.16
CZECHI/EiJ
30
283.08
26.40
PWD/PhJ
30
281.40
32.24
PERA/EiJ
255
219.16
23.06
WSB/EiJ
85
224.30
27.12
Mus spicilegus
32
266.23
16.21
Mus caroli
30
299.18
20.94
Rattus norvegicus
29
303.40
18.84
Peromyscus maniculatus
126
244.66
31.57
Microtus pennsylvanicus
44
244.84
24.20
Species
Standard Deviation
Mus musculus castaneus
Mus musculus musculus
Mus musculus domesticus
6 SI
B. L. Dumont and B. A. Payseur
TABLE S3
Synaptonemal complex lengths in F1 house mice
Cross
Number of Cells
Mean Synaptonemal
(Mother x Father)
Measured
Complex Length (μm)
WSB x CAST
30
231.67
21.77
CAST x WSB
30
245.14
20.43
Standard Deviation
Intersubspecific
WSB x PWD
120
241.36
21.34
PWD x WSB
29
232.96
21.07
CAST x PWD
30
275.55
21.80
PWD x CAST
30
251.16
27.54
WSB x PERA
81
228.09
17.07
PERA x WSB
142
227.84
18.64
PWD x CZECHI
30
293.51
27.46
CZECHI x PWD
30
260.56
24.45
Intrasubspecific