Material Safety Data Sheet
HeLa Lysate Control Kit
1. Chemical Product and Company Identification
Product Identification
Product Name
Other Names
Chem. family/formulae
HeLa Lysate Control Kit
HeLa Lysate Control Kit Bicine/CHAPS p/n. CBS404
HeLa Lysate Control Kit RIPA p/n. CBS400
Proprietary Buffers
Company Identification
Company
Address
Telephone No.
proteinsimple
3040 Oakmead Village Pkwy, Santa Clara, CA 95051
408-510-5500
Emergency No. 408/510-5500
2. Composition/Information on Ingredients
Chemical Name
DMSO Inhibitor Mix
Component 1:
CAS Number
Proprietary Mixture
Hazard Class
Irritant
Chemical Name
Aqueous Inhibitor Mix
Component 2:
CAS Number
Proprietary Mixture
Hazard Class
Toxic
Component 3:
Chemical Name
Lysis Buffer (RIPA)
CAS Number
Proprietary Mixture
Hazard Class
irritant
Component 4:
Chemical Name
Lysis Buffer (Bicene/CHAPS)
CAS Number
Proprietary Mixture
Hazard Class
irritant
Component 5:
Chemical Name
High & Low phospho HeLa
Lysate
CAS Number
HeLa cultured cells from ATCC
(no CAS #)
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Hazard Class
Possible pathogen
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HeLa Lysate Control Kit MSDS
3. Hazards Identification
The heart, skeleton, circulatory system, central nervous system, and kidneys are target
organs of some ingredients in the HeLa Lysate Control Kit. Some ingredients can
cause irritation to skin, eyes and respiratory tract.
Primary entry routes are exposure of mucous membranes to droplets/aerosols, possible
parenteral inoculation, inhalation, eye and skin contact.
Some of the ingredients in the HeLa Lysate Control Kit are still being investigated as
human carcinogens.
Potential Health Effects
Acute and Chronic:
Some ingredients in the Aqueous inhibitor are classified as toxic. Certain ingredients in
the DMSO inhibitor and Lysis Buffers, are classified as corrosive or irritant chemicals.
Avoid breathing dust or fumes.
The High and Low Phospho HeLa Lysates have a slight possibility of pathogenicity
4. First Aid Measures
Swallowed
Wash out mouth with water. Remove any dentures. Move victim to
fresh air. Keep warm and at rest.
If victim is conscious, give small quantities of water; stop if victim
feels sick; vomiting may be dangerous. Do not induce vomiting
unless directed to do so by medical personnel. If vomiting occurs, the
head should be kept low so that vomit does not enter the lungs.
A physician must treat chemical burns promptly. Never give
anything by mouth to an unconscious person. If unconscious, get
medical attention immediately.
Maintain an open airway. Loosen tight clothing such as a collar, tie,
belt or waistband. Do not induce vomiting if victim is conscious.
If the buffer swallowed is the Aqueous Inhibitor, administer milk,
chewable calcium carbonate tablets or milk of magnesia. GET
IMMEDIATE MEDICAL ATTENTION.
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Eye
HeLa Lysate Control Kit MSDS
• Immediately flush eyes with plenty of water for at least 15
minutes, occasionally lifting the upper and lower eyelids.
• Check for, and remove, any contact lenses.
• A physician must treat chemical burns promptly.
• GET IMMEDIATE MEDICAL ATTENTION..
•
Skin
•
•
•
•
•
•
•
Inhaled
•
•
•
•
•
For Rescuers
•
First Aid
Facilities
Flush skin with plenty of water. Continue to rinse for at least 10
minutes.
Remove contaminated clothing and shoes.
Wash thoroughly with water before removing, or wear gloves.
GET IMMEDIATE MEDICAL ATTENTION.
A physician must treat chemical burns promptly.
Wash clothing before reuse.
Clean shoes thoroughly before reuse.
Move victim to fresh air. If fumes could still be present,
rescuer(s) should wear appropriate mask or self-contained
breathing apparatus.
Keep victim warm and at rest. If not breathing, or if breathing
is irregular or if respiratory arrest occurs, a trained person
should administer artificial respiration, or oxygen.
It may be dangerous to give mouth-to mouth resuscitation. If
victim is unconscious, GET IMMEDIATE MEDICAL
ATTENTION. The victim may need medical surveillance for
48 hours
Maintain an open airway. Loosen tight clothing such as a
collar, tie, belt or waistband.
In case of inhalation of decomposition products in a fire,
symptoms may be delayed.
No action should be taken involving any personal risk, or without
suitable training.
If it is suspected that fumes are still present, rescuers should wear
appropriate mask or self-contained breathing apparatus.
It may be dangerous to give mouth-to-mouth resuscitation. Wash
contaminated clothing thoroughly with water before removing or
wear gloves.
• Plumbed eye wash
• Plumbed safety shower
• Chemical Fume hood
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Advice to
Physician
HeLa Lysate Control Kit MSDS
Treatment for overexposure should be directed at control of
symptoms and clinical conditions
5. Fire Fighting Measures
Flash Point/Method
Flammability Limits in Air
General Hazard
No information
No information
Flammable if exposed to fire or flame
Extinguishing Media
Do not use water. Use CO2.or dry chemical media for
small fires. Apply alcohol-type or all-purpose foams for
large fires.
Special Fire Fighting
Procedures
Fire fighters should use self-contained breathing
apparatus and body covering protective clothing.
Unusual Fire & Explosion
Hazards
At decomposition point, toxic fumes are released.
6. Accidental Release Measures
Spills and
Disposal
Wear safety goggles, appropriate gloves and lab coat or apron. Sweep up
or vacuum with a designated device. Avoid generating dust. Soak up
liquid with inert absorbent material. Wipe up liquid with appropriate
materials
Transfer contaminated material to suitable containers for disposal.
Affected surfaces should be decontaminated (see “Decontamination
Solution” below), then rinsed thoroughly with clean water. DO NOT add
decontamination solution to waste container. DO NOT use cotton waste,
sawdust or other combustible substances as absorbent materials, as these
may ignite when dried out.
Decontamination Solution; prepare a fresh solution of 5% sodium bicarbonate and 5%
sodium hypochlorite in water. Use at a ratio of 10 volumes decontamination solution per
estimated volume of residual spill
Dispose per local, state and Federal regulations
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HeLa Lysate Control Kit MSDS
7. Storage And Handling
 Keep all containers cool and dry. Keep away from heat and strong oxidizers.
 Avoid repeated or prolonged exposure.
 Avoid skin and eye contact.
 Use all safety precautions for handling hazardous chemicals. Observe safe
manufacturing and handling procedures
 Wash thoroughly after handling.
 Observe precautions as in the handling of any chemical substances.
8. Exposure Controls/Personnel Protection
Engineering
Controls
Use in a chemical fume hood, with air supplied by an
independent system. Use Biosafety Level 2 practices and
containment.
Personal
Protection
Safety glasses with side shields or safety goggles. If there is
risk of inhaling dust or powders, wear a NIOSH/OSHA
approved respirator).
To protect skin, wear PVC coated gloves and lab coat or
apron; gauntlets are suggested. Wash hands before eating ,
drinking, smoking, and/or applying cosmetics or other
substances.
Exposure
Standards
There are no OSHA PEL’s, ACGIH TLV’s or STEL’s for any of the
components of the Buffers.
PEL: Permissible Exposure Limits
STEL: Short Term Exposure Limit
TLV: Threshold Limit Value (the exposure standard term used by the American
Conference of Governmental Industrial Hygienists - ACGIH)
mg/m3: milligrams per cubic meter
9. Physical and Chemical Properties
Substance
DMSO
Inhibitor Mix
Aqueous
Inhibitor Mix
Lysis Buffers
High & Low
phospho
HeLa Lysate
Form
Boil Pt.
Mol. Wt.
Evp. Rt
Sp. Gr.
Soluble
In H20
pH
Volatility
Clear Liquid No info
No info
No info
No info
No info
No info No info
No info
No info
No info
No info
No info
No info
No info No info
No info
No info
No info
No info
No info
No data
No info No info
No info
No info
No info
No info
No info
No info
No info No info
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HeLa Lysate Control Kit MSDS
10. Stability and Reactivity
Stability
Stable
Chemical Stability
Hazardous polymerization will not occur
Heat, strong oxidizers. Heating in the presence of air
(oxygen) to temperatures above 212˚ C will result in
decomposition.
Incompatibilities
Products of Decomposition
Burning can produce oxides of carbon, nitrogen, sulfur and
mercury
11. Toxicological information
Some components, in the HeLa Lysate Control Kit, the DMSO Inhibitor mix and the
Antibody Diluent specifically, at full strength, would be regulated under Japan’s
Poisonous Substances Control Law.
12. Ecological Information
No information available
13. Disposal Considerations
Disposal must be in accordance with Federal, State and local regulations.
If approved, waste product may be transported to an approved disposal site.
Do not dispose of product down drains.
CERCLA (USA) No reportable quantities
14. Transport Information
Not regulated as a hazardous material
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HeLa Lysate Control Kit MSDS
15. Regulatory Information
US Federal Regulations
TSCA Inventory
Acute
Chronic
Fire
Reactivity
Pressure
SARA EHS List
CERCLA Hazardous Material
Sara Toxic Chemical List
Certain ingredients in the Buffers are included in
the inventory
Yes
Yes
Flammable in the presence of fire or flame
Slight
No
No
List
No
16. Other Information
Prepared By: proteinsimple
Disclaimer: Judgments as to the suitability of information herein for the purchaser’s
purposes are necessarily the purchaser’s responsibility.
Although reasonable care has been taken in the preparation of such information,
proteinsimple extends no warranties, makes no representation, and assumes no
responsibility as to the accuracy or suitability of such information or application to
the purchaser’s intended purpose or for consequences of its use.
Revision Number
Original final draft
Date of issue
July 2011
Replaces
Copyright © July 2011 proteinsimple. Any commercial use or reproduction without the publisher’s
permission is prohibited.
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