microscopenotes.notebook
September 25, 2015
Thursday, October 1
Bell Work:
a. What organelle is responsible for making proteins?
b. Which organelle is considered the control center of a
cell?
c. Which organelle is responsible for cleaning up old cell
parts, waste, and other materials in the cell?
d. Which organelle that is found in BOTH provides the
rest of the cell with its Energy?
e. Which organelle acts as the storage center for water
and other nutrients?
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Microscope
Compound
Light
1500x
living
preserved
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Electron
100,000x
protein molecules
living specimen
vacuum
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Scanning (SEM)
metal
electrons
3-D
Transmission (TEM)
2D
magnification
colorized
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TEM­ of stomata of a leaf­ original and colorized. Magnification 5000x
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SEM of stomata in a leaf. Magnification 1500x
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When carrying any microscope, always carry it by the __BASE______ and the ___ARM________.
The microscope that we will be using in the lab is the __COMPOUND_____ ___LIGHT_______ microscope which we will be using to see through objects (transparent). Biologists use this microscope to look into cells.
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Compound Light Microscope
Microscope used to see transparent (see through) objects like cells and water.
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• Eyepiece­ the part of the microscope that you look through to view the object.
• Body Tube­ connects the eye piece to the objective lenses
• Arm­ Supports the tube and connects it to the base
• Stage­ The part the microscope slide lays on
• Coarse Adjustment Knob­ Large, located on the side, used for focusing the specimen
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• Fine Adjustment Knob­ Small, located on the side, used to fine­tune focus specimen
• Light Source­ allows light to reflect upward through the diaphragm, the specimen, and the lens
• Revolving nose piece­ allows you to rotate the objectives
• Scanning Lens­ lowest power lens (shortest) used to find objects­ 4x
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• Low­power objective­ magnifying power of 10x
• High­power objective­ magnifying power of 40x
• Diaphragm­ regulates the amount of light entering the microscope
• Base­ the bottom of the microscope, used for support
• Stage Clips­ hold microscope slide in place
• Field of View – the amount of an object you can see while looking into the microscope
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Magnification
Magnification is the measure of how much larger the object appears through the microscope than it actually is. For example if you are looking at leaf using a stereomicroscope with 3x magnification, the parts of the leaf will appear 3 times larger than they actually are. Magnification can be determined by multiplying the magnification of each lens used to see the object.
10x X 4x = 40x
magnification magnification total of eyepiece of objective magnification
Complete the chart below to calculate the total magnification of an object.
4x
1. Which combination of lenses would allow you to see the object with greatest magnification? ____________
2. Which combination of lenses would allow you to see the largest area of the specimen? ________________
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