Unive.sity of Ljubljana
prof. dr. StanislavTrdan
Biotechnical
Chair f or Phytomedicine, Agricultural
fuculty
Engineering, Crop Production,
Pasture and Grassland Management
Jamnikarjeva 101
S!1000 Ljubljana, Slovenia
tel:003851320 32 25
faks:003861256 37 70
e-maili stanislav.trdan@bf .uni-li.si
oatei uth Nov, 2016
Statement Partial rcpolton testing of Mineral .ed wilh essentlal oils
Accordihg to the initiative ofAs an d.o.o. enterprise field testing of Mineral products, mode of action
has been made.
A.
BASIC DATA
ln May 2016, As an d.o.o. (further known as applicant) has shown interest for testine Mineral red
(Mineralxwhich was presented as insecticide and growing stimulator). purpose of testing was
Mineral's mode of action against different insect pests on d iffe rent vegeta ble plants.
Researchers that were working on research are: assist. dr. Tanja Bohing prof. dr. Stanislav Trdan,
laka Rupnik.
We have tested an impact of product )Mineral red( { with essehtial oils of clove, oil rape, lavender,
common juniper and eucalyptus), in 9 % concentration.
All experiments took place at the LaboGtory Field, Department of agronomy, Biotechncial faculty,
Jamnikarjeva 101, Ljubl.iana
B.
FIELD EXPERIMENT
wlfB
LEEK
lAlliun porrun L.)
We have tested the impad of 'Mineral red', with essential oil of clove, lavender, common juniper,
eucalyptus and oil rape. Positive and negative controlwere also part ofthe experiment.
Experiment was divided into 7 treatments. (Mineral red with clove, mineral red with eucalyptus,
mineral red with lavender, mineral red with common.iuniper, mineral red with oil rape,positive and
ne8ative control). Plants were sprayed with Mineral red with different essential oils
in 8
%
concentration. Plants were sprayed on 2oth June, 15th July, 29s August, 56 August, 12th August, 25th
August, 6s September and l5sSeptember. On treatment ,Chemical treatment(, insecticide ,Karate
Zeon 5 C5' was only used twice, on sih August and 6th September at the concentration of 0.15 l/ha.
Damage caused by onion thrips {Ihrlps toboci Lindeman) was evaluated on central rows. We have
evaluated damage caused by thrips tabaci according to evaluation scale described by Riahter et al.
(1999). The damage scale was evaluated as followsr 1= no damage,2= till20 % damaged leafarea, 3=
27-33%;4=34-50% and 5 presents above 50 % damaged leaf area.
Planting distance was 0,30 cm x 0,30 cm,
81. RESULTS
4,5
3.
3,s
93
E
7
Eo
clove
Eolavender
Eo
chemical
common Control(nou
iuniper
Eo
eucalyptus
Eo
oilrap€
reated)
Figure 1: Average index of injuries by thrips on leek (tSE) (upper case letters present statistically
signlf icant differences between treatments)
!:
E.
110
I
120
{
1oo
a
E60
p
conkol(non
ch.mical
Eo
eucatvotus
Eo
oll 6Pe
reated)
(in*clicide,
Figure 2: Average gross mass
treahnents)
of leek plant (iSE) {upp€I
case lefiels present differences between
/
FiBure 3: Field experiment with leek (photo: T. Bohinc)
Figure 4: lnjuries caused by onion thrips (Ihrips toboci Lindeman) on leek (tsE)
n
250
E
:
6
2oo
t1
E
I
E
150
1OO
E
E.o
g
E
g
o
Eolavender tocommon
junaper
Controllnon Chemical
treated)
Eo
eucaltltus
Eo
oilrape
treathenr
(inse.ticide,
FiSure 5: Average net mass od leek plant (tSE) (lowercase letters present statistically significant
diff erences between treatments).
C.
FIELD EXPERIMENT
wlIH
CARROT
We have tested the impad of rMineral red', with essential oil of clove, lavender, common juniper,
eucalyptus . Positive and negative control were also part ofthe experiments
Plants were sprayed on l1th July,25th July and 8th August, and watered on 30th June, 18th July,
25th July, 22nd July, 5-9. and 14.9., On treatment ,Chemical treatment(, insecticide ' Actara 25 WG'
was only used once, on 17th lune, at sowin8.
Figure 6: Car.ot plants, where experimentwas performed (photo:T. Bohinc)
Experiment was performed on variety 'Nantaise amelioree 2'.
C1, RESULTS
EEr,s
'i
f E
cg
$t
E
1
nq
Eo
clove
Eo
lavander
Eo
common
Eo Eucalyptus
juniper
chemical
treatment
Figure 6: Avera8e number of holes c6used by wireworms {tSE) {lower case letters present differences
between treatments)
D.
.
FIEID EXPERIMENT WITH ONION (Arlium cepo L.)
We have tested the impact of 'Mineral red', with essential oil of clove, lavender. Common juniper,
eucalyptus and oil rape. Positive and negative control were also one ofthe treatments.
Experiment was divided ihto 7 treatments. (Mineral red with clove, mineral red with eucalyptus,
mineral red with lavender, mineral red with common junipet mineral red with oil rape,positive and
negative control). Planrs were sprayed with Minereal red with different essential oils in 8 %
concentration.
Plants were sprayed on 2fth Ji.rne, 30th June, 7th July, 10th tuly, 15th July, 2fth July, 27th luly and
10th Au8ust. On treatment )Chemical treatment(, insecticide 'Karate Zeon 5 CS' was only used three
times, on 2othlune, Tth July and 2oth July.
Damage caused by onion thrjps lThrips tdboci Lindeman) was evaluated on central rows, We have
evaluated dama8e caused by thrips tabaci according to evaluation scale described by Richter et al.
(1999), The damage s.ale was evaluated as follows: 1= no damage, 2= till 20 % damaged leafa.ea.3=
27-33 %;4= 34-50 % and 5 presents above 50 % damaged leaf area.
Planting denslty was 0.25 cm * 0.25 cm.
Planting was performed on 196 May, harvestingwas performed on 3Os August 2016.
Dl.
RESUt"ts
?.E,
common Eo
Chemical
juniper eucalyptus treatment
Eo
Eo
clove
Eo
oilrape
Eo
lavender
{insecticides)
Treatment
Figure 7: Average index of injuries by onion thrips on onion plants (lSE) (upper case letters present
diff erences between treaments)
120
E
100
30
a
E
2o
0
Eo
eucalyptus
Chemical
Eooilrape
Eol&ender
treatnent
(insedlcide9
Figure 8: Average mass
treaments).
of onion bulbs {tSE) (upper
case letters present differences between
Figure 9: Field experiment with onion (photo: T. Bohinc)
E.
FIELD EXPERIMENTwlIH CABBAGE
Experiment was performed on cultivar'Sweety F1'. Plants were transplanted into the open field on
23td May,20L6.
Cabbage plants were sprayed
with Mineral .ed with essential oil of oil rape, mineral red with
essential oil of lavender and mineral red with essential oil of common juniper. Positive control
(chemical treatment) was sprayed with Bulldock
EC
25 (5 ml/101 Hro)and Fastac 100 EC (0.75
ml/10I
Hz0).
iormulations (8 % concentration) mentioned above on 15s June, 2oth lune,
3o'h June,7'h.,uly, 11'h July,27hJuly, 10$ August, 17$ August 5s September. The damage due to the
cabbage flea beetles was evaluated using the s-grade European and lvlediterranean Plant Protection
Organization (EPPO) scale {OEPP/EPPO, 2002). The plants were evaluated on a scale from 1 (no
damage) to 5 (more than 25% leaf area eaten), as follows: (2) up to 2% leaf area eaten, (3) between
Plants were sprayed with
3% and 10% leaf area eaten and (4) 11-25% leaf area eaten.
Planting density was 0.30*0,40 cm.
/,
5
a
2
Eooi'doe Lo.ommon.Lnp.r Lolaverder Cl.ef,cal.oltol
FiSure 10: Average index of injuries caused by Phyllotrcto spp. on cabbage (1SE) (upper case letters
present differences between treaments).
500
aE
E
a
i1
E
450
4oo
t00
__-
T
200
E
rso
g
100
lso
l
to
Eooilrape
Eocommon Eolavender Chemical.ontrol
Contol
juniper
j
rl
Treatment
l
Figure 11r Average net mass (tSE) (in gramms/treatment) (upper case letters present differences
between treatments).
F;gure 12: Field experiment with cabbage (photo: T. Bohinc)
F.
EXPERIMENT ON BEAN
Experiment was divided into 7 treatments. (Mineral red with clove, mineral red with eucalyptus,
mineral red with lavender, mineral red with common juniper, mineral red with oil rape,positive and
negative control). Plants were sprayed with )Mineral red< with different essential oils in 8 %
concentration. Number ofaphids was counted in four different dates.
Che m ica I
treatment was treated with Sulldock
EC
We have planted been on 19th May.
FiSure 13: Experiment on bean (photo: T. gohinc)
25, on 3oth June, and 8th July.
F1. RESU LTS
160
G. 140
!
100
E8o
o
z
<40
Eo
Eo
lavender Io common
Eo
oilrape
juniper
Figure
14:
Average number of aphids/plant (15E1 {upper case letters present differences between
treatments).
G.
FINAI. OPINION
Aim of our research was to test impact of 'Mineral red' improved wlth different essential oils (clove,
lavender, eucalyptus, commonjuniper and oll rape).
Research was performed on different vegetables plants.
Based on our results we can conclude that rMineral red'in combination with oil rape's essential oil
and common juniper's essential oil can be effective against onion thrips.
Additional tests should be done on impact of 'Mineral red' with eucalyptus's essential oil.
Common junipeas and clove's essentialoils prooved to be effective also against wireworms.
Oil rape's essential oilwas effective also against injuries caused by Phyl/otreto spp.
Hgad of !.G q[e!r of Phvtomedici,!e
Arricultural Enerneerinp. CroD Produ.tion
e"'r{i"u n.","rd M";;".*,
Prof. dr.SrenhlavTrdan
-"
Vice
d33!
BF
of Asrgr.g.Fr
A-T-i
Prof. dr. Miha Humar