PRODUCT INFORMATION
Myeloperoxidase (mouse) Polyclonal Antibody
Item No. 20493
Overview and Properties
Contents:
This vial contains 500 µl of protein A-purified IgG antibody.
Synonym:MPO
Immunogen:
Full length recombinant mouse MPO
Species Reactivity:(+) Mouse MPO
Uniprot No.:P11247
Form:Liquid
Storage:
-20°C (as supplied)
Stability:
As supplied, 1 year from the QC date provided on the Certificate of Analysis, when
stored properly
Storage Buffer:
TBS, pH 7.4, containing 50% glycerol, 0.1% BSA, and 0.02% sodium azide
Host:Rabbit
Applications:
ELISA and Western blot (WB); the recommended starting dilution for ELISA is 1:500
and 1:200 for WB. Other applications were not tested, therefore optimal working
concentration/dilution should be determined empirically.
Image
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· · · · · · · 80 kDa
Lane 1: MPO (mouse recombinant) (10 ng)
Lane 2: MPO (mouse recombinant) (25 ng)
Lane 3: MPO (mouse recombinant) (50 ng)
Lane 4: MPO (mouse recombinant) (100 ng)
WARNING
THIS PRODUCT IS FOR RESEARCH ONLY - NOT FOR HUMAN OR VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.
SAFETY DATA
This material should be considered hazardous until further information becomes available. Do not ingest, inhale, get in eyes, on skin, or on clothing. Wash thoroughly after
handling. Before use, the user must review the complete Safety Data Sheet, which has been sent via email to your institution.
WARRANTY AND LIMITATION OF REMEDY
Buyer agrees to purchase the material subject to Cayman’s Terms and Conditions. Complete Terms and Conditions including Warranty and Limitation of Liability information
can be found on our website.
Copyright Cayman Chemical Company, 10/27/2016
CAYMAN CHEMICAL
1180 EAST ELLSWORTH RD
ANN ARBOR, MI 48108 · USA
PHONE: [800] 364-9897
[734] 971-3335
FAX: [734] 971-3640
[email protected]
WWW.CAYMANCHEM.COM
PRODUCT INFORMATION
Description
Myeloperoxidase (MPO) is one of the most abundant proteins in neutrophils comprising 5% of the dry
weight of the cell. 1 MPO is stored in the azurophilic granules and released when neutrophils are stimulated.
MPO catalyzes the oxidation of chloride and other halide ions in the presence of hydrogen peroxide
to generate hypochlorous acid and other antimicrobial products.2,3 MPO is required for the neutrophil
extracellular trap (NETs) response.4 Activated neutrophils die and release NETs which are composed
of decondensed chromatin and anti-microbial proteins including MPO.5,6 The predicted size of MPO is
81.2 kDa and Cayman’s MPO (mouse) Polyclonal Antibody detects a band at approximately 80 kDa by
western blot.
References
1. Schultz, J. and Kaminker, K. Myeloperoxidase of the leucocyte of normal human blood.1 I. Content and
localization. Arch. Biochem. Biophys. 96, 465-467 (1962).
2. Harrison, J. E. and Schultz, J. Studies on the chlorinating activity of myeloperoxidase. J. Biol. Chem. 251(5),
1371-1374 (1976).
3. Klebanoff, S. J. Myeloperoxidase: Friend and foe. J. Leukoc. Biol. 77(5), 598-625 (2005).
4. Metzler, K. D., Fuchs, T. A., Nauseef, W. M., et al. Myeloperoxidase is required for neutrophil extracellular
trap formation: Implications for innate immunity. Blood 117(3), 953-959 (2011).
5. Fuchs, T. A., Abed, U., Goosman, C., et al. Novel cell death program leads to neutrophil extracellular traps.
J. Cell Biol. 176(2), 231-241 (2007).
6. Brinkmann, V., Reichard, U., Goosmann, C., et al. Neutrophil extracellular traps kill bacteria. Science
303(5663), 1532-1535 (2004).
CAYMAN CHEMICAL
1180 EAST ELLSWORTH RD
ANN ARBOR, MI 48108 · USA
PHONE: [800] 364-9897
[734] 971-3335
FAX: [734] 971-3640
[email protected]
WWW.CAYMANCHEM.COM