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Thermostable Inorganic Pyrophosphatase
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Catalog #
M0296S
Size
250 units
Concentration
2,000 units/ml
Price
$70.00
Qty
M0296L
1,250 units
2,000 units/ml
$280.00
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1
Categories: Phosphatase & Sulfurylase Products, RNA Modification Products
Applications: In vitro Synthesis & Processing
Product
Information
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Tech Tips
Protocols &
Manuals
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Description
Related Products
Quality &
Safety
Legal
Information
Properties and Usage
References
Description
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate:
P2O7-4 + H2O -> 2HPO4-2.
Highlights
Retains 100% activity after incubation at 100°C for 4 hours
Product Source
An E. coli strain carrying a plasmid encoding a pyrophosphatase from the extreme thermophile Thermococcus litoralis.
Properties and Usage
Unit Definition
One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction
conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).
Storage Temperature
-20°C
Storage Conditions
20 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8.0 @ 25°C
Heat Inactivation
No
Related Products
Companion Products
Monarch® Plasmid Miniprep Kit
Monarch® DNA Gel Extraction Kit
Monarch® PCR & DNA Cleanup Kit (5 μg)
References
1. Heinonen, J.K. and Lahti, R.J. (1981). Analytical Biochemistry. 113, 313-317.
FAQs
FAQs
1. What is the activity of TIPP at 37°C?
Datacards
Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and
quality controls. The following file naming structure is used to name the majority of these document files: [Catalog
Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the
Technical Support Form for appropriate document.
M0296Datasheet-Lot0091204
M0296Datasheet-Lot0091208
M0296Datasheet-Lot0091303
M0296Datasheet-Lot0091212
M0296Datasheet-Lot0091305
M0296Datasheet-Lot0091310
M0296Datasheet-Lot0091406
M0296Datasheet-Lot0091409
M0296Datasheet-Lot0091411
M0296Datasheet-Lot0091501
M0296Datasheet-Lot0091504
M0296Datasheet-Lot0091512
M0296Datasheet-Lot0091609
M0296Datasheet-Lot0091612
Interactive Tools
Interactive Tools
NEBioCalculator
Quality Control
Safety Data Sheet
Datacards
Quality Control
Quality Control Assays
The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product.
Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting
Documents section of this page. Further information regarding NEB product quality can be found here.
dNTPase Activity:
The Pyrophosphatase is tested in a reaction containing a mix of dNTP's. After incubation the amount of inorganic phosphatase
released is determined by comparison to a standard curve of known phosphate concentrations.
Endonuclease Activity (Nicking):
The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to
the nicked form is determined by agarose gel electrophoresis.
Exonuclease Activity (Radioactivity Release):
The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4
hours the exonuclease activity is determined by the % release of radioactive nucleotides.
Non-Specific DNase Activity (16 hour):
The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16
hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
Phosphatase Activity (PNPP):
The product is tested in a reaction containing a p-nitrophenyl phosphate (PNPP), a chromogenic substrate for most
phosphatases. After incubation the percent degradation is determined by spectrophotometric analysis of released p-nitrophenol
at 405 nm.
Safety Data Sheet
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
Thermostable Inorganic Pyrophosphatase
Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and
quality controls. The following file naming structure is used to name the majority of these document files: [Catalog
Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the
Technical Support Form for appropriate document.
M0296Datasheet-Lot0091204
M0296Datasheet-Lot0091208
M0296Datasheet-Lot0091303
M0296Datasheet-Lot0091212
M0296Datasheet-Lot0091305
M0296Datasheet-Lot0091310
M0296Datasheet-Lot0091406
M0296Datasheet-Lot0091409
M0296Datasheet-Lot0091411
M0296Datasheet-Lot0091501
M0296Datasheet-Lot0091504
M0296Datasheet-Lot0091512
M0296Datasheet-Lot0091609
M0296Datasheet-Lot0091612
Legal and Disclaimers
Legal and Disclaimers
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc
(NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain
additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes
in humans or animals.